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The map label for this gene is nuoF2 [H]

Identifier: 86747851

GI number: 86747851

Start: 815043

End: 816599

Strand: Reverse

Name: nuoF2 [H]

Synonym: RPB_0725

Alternate gene names: 86747851

Gene position: 816599-815043 (Counterclockwise)

Preceding gene: 86747852

Following gene: 86747850

Centisome position: 15.32

GC content: 68.66

Gene sequence:

>1557_bases
ATGAGCCTGCGCGTCTTCATCCCGCGCGACTCCGGCGCGCTTGCGGTCGGCGCCGACGAGGCCGCGGCCGAACTCACCCG
CGTCGCCCATGACCGCCACCTGCCGCTCGATCTGGTGCGGCCCGGTTCGCGCGGGCTGTATTGGCTGGAGCCGATGCTCG
AAGTCGATACGCCGGCCGGCCGCGTCGCCTTCGGCCCGACCACAGCGGACGACGTGGCGTCGATCCTCGACGCGATTCTG
TCGGATGGCGAGCATCCGCTGAAGCTCGGCCTCACCGAACAGATCCCCTGGCTGAAGCGTCAGACCCGCCTCACCTTCGT
CCGCTGCGGCATCATCGATCCGCGCTCGATCGACGATTACCGGGCGCATGGCGGCTACAAGGGCCTCGAACGCGCGCTGA
AGATGAGCTCGGACGCGATCATCGACGACATCGTCAAATCCGGCCTGCGCGGCCGCGGCGGCGCCGGCTTCCCGACCGGC
ACCAAATGGCGCACCGTGGCGCAGGCCGGCCGCGGCCAGAAATACATCTGCTGCAACGCCGACGAGGGCGACAGCGGCAC
CTTCGCCGACCGCATGCTGATGGAGGGCGATCCCTTCATGGTGATCGAAGGCATGACGATCGCCGGCATCGCGGTCGGCG
CTACCCGCGGCTACATCTATATCCGCTCGGAATATCCGCATGCGGTCGAAGCCATGAATGCGGCGATCGAGGCGGCGCGC
CGCACCGGCTATCTCGGCGATCACATCTGCCATTCGACCGACTCCTTCGACATCGAAGTGAGGGTCGGCGCCGGCGCCTA
TATCTGCGGCGAGGAGACCTCGATGCTGGAAAGCCTCGAGGGCAAGCGCGGCACCGTCCGCGCCAAGCCGCCGCTGCCGG
CGCACAAGGGCCTGTTCGGCCGGCCCACCGTGATCAACAACGTGCTGACCTTCTCGGCGGTGCCGTACATTCTCGACGGC
GGCGCGCAGGCCTATGCGGATTGCGGCATGGGCCGCTCGCGCGGCACCAAGCCGATCCAGCTCGGCGGCAACATCAGGCA
CGGCGGGCTGTTCGAGGTCGCGTTCGGCATCACGCTCGGCGAACTGATCGACGACATCGGCGGCGGCACGCTGAGCGGCC
GCCCGGTGCGCGCCGTGCAGGTCGGCGGGCCGCTCGGCGCGTACTTCCCGCGTGCGCTGTTCGACACGCCGTTCGACTAC
GAGGCTTTTGCTGCGCGGAGCGGCATGGTCGGCCACGGCGGCGTGGTGGTGTTCGACGACACCGTCGACATGGCCAAGCA
GGCGCGGTTCGCGATGGAGTTCTGCGCGATCGAATCCTGCGGCAAATGCACCCCCTGTCGCATCGGCTCGACCCGCGGCG
CCGAGACCGTCGACAAGATCATCGCCGGCGACAGGCCTGCGGCCAACATCGCCGTGCTCGAAGACCTCTGCAACACCATG
AAGTTCGGCTCGCTGTGCGCGCTGGGCGGACTGGCGCCGATCCCGGTGCTCAGCGCGCTGGCGCACTTCCCGGAAGATTT
CGGCCTCGCGCCGCGCCGCCTGCAGGCTGCGGAATAA

Upstream 100 bases:

>100_bases
TCGGCCTGTGCGCCACGGCGCCGGCGGCGATGCTGAACGAGCGCGTGATCGGCCGGCTCGATGCCGGCAAGCTCGATGCG
CTGCTGACGGAGGCGCAGCC

Downstream 100 bases:

>100_bases
AGGACGCCCGATGGCTCTGGTTCACGAAACCGATTTCGGCACGCCGCGCTCGCCGTCCGAGACGATGGTGACGCTGACGA
TCGACGGCCGCAACGTCAGC

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MSLRVFIPRDSGALAVGADEAAAELTRVAHDRHLPLDLVRPGSRGLYWLEPMLEVDTPAGRVAFGPTTADDVASILDAIL
SDGEHPLKLGLTEQIPWLKRQTRLTFVRCGIIDPRSIDDYRAHGGYKGLERALKMSSDAIIDDIVKSGLRGRGGAGFPTG
TKWRTVAQAGRGQKYICCNADEGDSGTFADRMLMEGDPFMVIEGMTIAGIAVGATRGYIYIRSEYPHAVEAMNAAIEAAR
RTGYLGDHICHSTDSFDIEVRVGAGAYICGEETSMLESLEGKRGTVRAKPPLPAHKGLFGRPTVINNVLTFSAVPYILDG
GAQAYADCGMGRSRGTKPIQLGGNIRHGGLFEVAFGITLGELIDDIGGGTLSGRPVRAVQVGGPLGAYFPRALFDTPFDY
EAFAARSGMVGHGGVVVFDDTVDMAKQARFAMEFCAIESCGKCTPCRIGSTRGAETVDKIIAGDRPAANIAVLEDLCNTM
KFGSLCALGGLAPIPVLSALAHFPEDFGLAPRRLQAAE

Sequences:

>Translated_518_residues
MSLRVFIPRDSGALAVGADEAAAELTRVAHDRHLPLDLVRPGSRGLYWLEPMLEVDTPAGRVAFGPTTADDVASILDAIL
SDGEHPLKLGLTEQIPWLKRQTRLTFVRCGIIDPRSIDDYRAHGGYKGLERALKMSSDAIIDDIVKSGLRGRGGAGFPTG
TKWRTVAQAGRGQKYICCNADEGDSGTFADRMLMEGDPFMVIEGMTIAGIAVGATRGYIYIRSEYPHAVEAMNAAIEAAR
RTGYLGDHICHSTDSFDIEVRVGAGAYICGEETSMLESLEGKRGTVRAKPPLPAHKGLFGRPTVINNVLTFSAVPYILDG
GAQAYADCGMGRSRGTKPIQLGGNIRHGGLFEVAFGITLGELIDDIGGGTLSGRPVRAVQVGGPLGAYFPRALFDTPFDY
EAFAARSGMVGHGGVVVFDDTVDMAKQARFAMEFCAIESCGKCTPCRIGSTRGAETVDKIIAGDRPAANIAVLEDLCNTM
KFGSLCALGGLAPIPVLSALAHFPEDFGLAPRRLQAAE
>Mature_517_residues
SLRVFIPRDSGALAVGADEAAAELTRVAHDRHLPLDLVRPGSRGLYWLEPMLEVDTPAGRVAFGPTTADDVASILDAILS
DGEHPLKLGLTEQIPWLKRQTRLTFVRCGIIDPRSIDDYRAHGGYKGLERALKMSSDAIIDDIVKSGLRGRGGAGFPTGT
KWRTVAQAGRGQKYICCNADEGDSGTFADRMLMEGDPFMVIEGMTIAGIAVGATRGYIYIRSEYPHAVEAMNAAIEAARR
TGYLGDHICHSTDSFDIEVRVGAGAYICGEETSMLESLEGKRGTVRAKPPLPAHKGLFGRPTVINNVLTFSAVPYILDGG
AQAYADCGMGRSRGTKPIQLGGNIRHGGLFEVAFGITLGELIDDIGGGTLSGRPVRAVQVGGPLGAYFPRALFDTPFDYE
AFAARSGMVGHGGVVVFDDTVDMAKQARFAMEFCAIESCGKCTPCRIGSTRGAETVDKIIAGDRPAANIAVLEDLCNTMK
FGSLCALGGLAPIPVLSALAHFPEDFGLAPRRLQAAE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=397, Percent_Identity=39.0428211586902, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI260656005, Length=397, Percent_Identity=39.0428211586902, Blast_Score=253, Evalue=2e-67,
Organism=Escherichia coli, GI1788620, Length=398, Percent_Identity=38.4422110552764, Blast_Score=287, Evalue=9e-79,
Organism=Caenorhabditis elegans, GI17531727, Length=397, Percent_Identity=38.7909319899244, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI19920794, Length=397, Percent_Identity=39.0428211586902, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI45550452, Length=367, Percent_Identity=37.874659400545, Blast_Score=232, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24653819, Length=380, Percent_Identity=30, Blast_Score=171, Evalue=8e-43,
Organism=Drosophila melanogaster, GI24653817, Length=332, Percent_Identity=28.3132530120482, Blast_Score=140, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55109; Mature: 54977

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRVFIPRDSGALAVGADEAAAELTRVAHDRHLPLDLVRPGSRGLYWLEPMLEVDTPAG
CCEEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCCEEEECHHHCCCCCCC
RVAFGPTTADDVASILDAILSDGEHPLKLGLTEQIPWLKRQTRLTFVRCGIIDPRSIDDY
CEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHCEEEEEEECCCCCCCCCHH
RAHGGYKGLERALKMSSDAIIDDIVKSGLRGRGGAGFPTGTKWRTVAQAGRGQKYICCNA
HHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECC
DEGDSGTFADRMLMEGDPFMVIEGMTIAGIAVGATRGYIYIRSEYPHAVEAMNAAIEAAR
CCCCCCCHHHHHHCCCCCEEEEECCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHH
RTGYLGDHICHSTDSFDIEVRVGAGAYICGEETSMLESLEGKRGTVRAKPPLPAHKGLFG
HCCCCCHHHHCCCCCEEEEEEECCCEEEECCHHHHHHHCCCCCCEEECCCCCCCCCCCCC
RPTVINNVLTFSAVPYILDGGAQAYADCGMGRSRGTKPIQLGGNIRHGGLFEVAFGITLG
CCHHHHHHHHHHCCCCEECCCCHHHHHCCCCCCCCCCEEEECCCCCCCCEEEHHHHHHHH
ELIDDIGGGTLSGRPVRAVQVGGPLGAYFPRALFDTPFDYEAFAARSGMVGHGGVVVFDD
HHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEC
TVDMAKQARFAMEFCAIESCGKCTPCRIGSTRGAETVDKIIAGDRPAANIAVLEDLCNTM
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
KFGSLCALGGLAPIPVLSALAHFPEDFGLAPRRLQAAE
HHCCHHHHCCCCHHHHHHHHHHCCHHHCCCCCHHCCCC
>Mature Secondary Structure 
SLRVFIPRDSGALAVGADEAAAELTRVAHDRHLPLDLVRPGSRGLYWLEPMLEVDTPAG
CEEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCCEEEECHHHCCCCCCC
RVAFGPTTADDVASILDAILSDGEHPLKLGLTEQIPWLKRQTRLTFVRCGIIDPRSIDDY
CEEECCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHCEEEEEEECCCCCCCCCHH
RAHGGYKGLERALKMSSDAIIDDIVKSGLRGRGGAGFPTGTKWRTVAQAGRGQKYICCNA
HHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEECC
DEGDSGTFADRMLMEGDPFMVIEGMTIAGIAVGATRGYIYIRSEYPHAVEAMNAAIEAAR
CCCCCCCHHHHHHCCCCCEEEEECCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHH
RTGYLGDHICHSTDSFDIEVRVGAGAYICGEETSMLESLEGKRGTVRAKPPLPAHKGLFG
HCCCCCHHHHCCCCCEEEEEEECCCEEEECCHHHHHHHCCCCCCEEECCCCCCCCCCCCC
RPTVINNVLTFSAVPYILDGGAQAYADCGMGRSRGTKPIQLGGNIRHGGLFEVAFGITLG
CCHHHHHHHHHHCCCCEECCCCHHHHHCCCCCCCCCCEEEECCCCCCCCEEEHHHHHHHH
ELIDDIGGGTLSGRPVRAVQVGGPLGAYFPRALFDTPFDYEAFAARSGMVGHGGVVVFDD
HHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCEEEEEC
TVDMAKQARFAMEFCAIESCGKCTPCRIGSTRGAETVDKIIAGDRPAANIAVLEDLCNTM
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
KFGSLCALGGLAPIPVLSALAHFPEDFGLAPRRLQAAE
HHCCHHHHCCCCHHHHHHHHHHCCHHHCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]