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The map label for this gene is polA [H]

Identifier: 86747785

GI number: 86747785

Start: 743528

End: 744142

Strand: Reverse

Name: polA [H]

Synonym: RPB_0659

Alternate gene names: 86747785

Gene position: 744142-743528 (Counterclockwise)

Preceding gene: 86747787

Following gene: 86747784

Centisome position: 13.96

GC content: 64.88

Gene sequence:

>615_bases
ATGACGATCCGCCTGCATCGCGGCGATCTGCCGGATCTCACCCGCTACAATGATTCCGTGGCGATCGACACCGAGACGAT
GGGGCTGCATCCGCATCGCGACCGATTGTGCGTGGTGCAATTATCGAACGGCGACGGCAGCGCTGACGTGGTGCAGATCC
CGCAGGGGCACAGCGATGCGCCGAACCTGAAGAAGCTGCTCGGCGACCCGAATATCTTGAAGATCTTCCACTTCGCGCGG
TTCGATCTCGCCGCGCTCTCCAATGCGCTCGGGGTGATGCCGCAACCGGTGTACTGCACCAAAATCGCCTCGCGGCTGAC
CCGGACCTACACCGACCGGCACGGCCTGAAGGACCTGGTGCGCGAGCTGCTCAACGTCGATCTGTCGAAGCAGCAGCAAT
CCAGCGACTGGGGCGCCGACACGCTGAGCGACGCCCAGCTCGCTTACGCCGCCTCCGACGTGCTTCATCTCCACGCGCTG
CGCGACAAGCTCGACGCCATGCTGGCGCGGGAGAACCGAACCGAGCTGGCGCAGGCCTGCTTCGGATTCCTGCCGCACCG
CGCCCGACTCGACCTCGGCGGCTGGAGCGAAGAGGACATCTTCGCGCATTCCTGA

Upstream 100 bases:

>100_bases
CGCCTCACGCCGGTCGCGGCGATAGGTTGAGCAGGCGATTCGAGGCCGGTTCCGGCCCCTCCTTCAACGCCCGATTTCAA
TTCCGAGGATTTGCCCCACC

Downstream 100 bases:

>100_bases
GGAAATCGGGCCTTCGCCGACGGGCAAGGCCCGCCCCGTTTCGGTCATACTCCTCGTCCTGATGGAGTTTTGATCGACCG
GACGCCGGTGTCCCCTCGCC

Product: 3'-5' exonuclease

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MTIRLHRGDLPDLTRYNDSVAIDTETMGLHPHRDRLCVVQLSNGDGSADVVQIPQGHSDAPNLKKLLGDPNILKIFHFAR
FDLAALSNALGVMPQPVYCTKIASRLTRTYTDRHGLKDLVRELLNVDLSKQQQSSDWGADTLSDAQLAYAASDVLHLHAL
RDKLDAMLARENRTELAQACFGFLPHRARLDLGGWSEEDIFAHS

Sequences:

>Translated_204_residues
MTIRLHRGDLPDLTRYNDSVAIDTETMGLHPHRDRLCVVQLSNGDGSADVVQIPQGHSDAPNLKKLLGDPNILKIFHFAR
FDLAALSNALGVMPQPVYCTKIASRLTRTYTDRHGLKDLVRELLNVDLSKQQQSSDWGADTLSDAQLAYAASDVLHLHAL
RDKLDAMLARENRTELAQACFGFLPHRARLDLGGWSEEDIFAHS
>Mature_203_residues
TIRLHRGDLPDLTRYNDSVAIDTETMGLHPHRDRLCVVQLSNGDGSADVVQIPQGHSDAPNLKKLLGDPNILKIFHFARF
DLAALSNALGVMPQPVYCTKIASRLTRTYTDRHGLKDLVRELLNVDLSKQQQSSDWGADTLSDAQLAYAASDVLHLHALR
DKLDAMLARENRTELAQACFGFLPHRARLDLGGWSEEDIFAHS

Specific function: Cleaves Multimeric tRNA Precursor At The Spacer Region. [C]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 3'-5' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788105, Length=158, Percent_Identity=32.9113924050633, Blast_Score=75, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR002298
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 22720; Mature: 22589

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRLHRGDLPDLTRYNDSVAIDTETMGLHPHRDRLCVVQLSNGDGSADVVQIPQGHSDA
CEEEECCCCCCCHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCC
PNLKKLLGDPNILKIFHFARFDLAALSNALGVMPQPVYCTKIASRLTRTYTDRHGLKDLV
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
RELLNVDLSKQQQSSDWGADTLSDAQLAYAASDVLHLHALRDKLDAMLARENRTELAQAC
HHHHCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FGFLPHRARLDLGGWSEEDIFAHS
HHHCCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TIRLHRGDLPDLTRYNDSVAIDTETMGLHPHRDRLCVVQLSNGDGSADVVQIPQGHSDA
EEEECCCCCCCHHHCCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCC
PNLKKLLGDPNILKIFHFARFDLAALSNALGVMPQPVYCTKIASRLTRTYTDRHGLKDLV
CHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHH
RELLNVDLSKQQQSSDWGADTLSDAQLAYAASDVLHLHALRDKLDAMLARENRTELAQAC
HHHHCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
FGFLPHRARLDLGGWSEEDIFAHS
HHHCCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]