Click here to switch to the map view.

The map label for this gene is sppA [C]

Identifier: 86747765

GI number: 86747765

Start: 723235

End: 724200

Strand: Reverse

Name: sppA [C]

Synonym: RPB_0639

Alternate gene names: 86747765

Gene position: 724200-723235 (Counterclockwise)

Preceding gene: 86747766

Following gene: 86747764

Centisome position: 13.58

GC content: 67.08

Gene sequence:

>966_bases
ATGTCGCTTGATTCCGATGTGATCGTCGATCGCCGCCGCATTCGTCGCAAGCTGACGTTCTGGCGGGTCGCCGCGGTTCT
GGTCGCGATCGGCGCCGTGATCGCGGTCGGCGCGATGGTGTCGCCGGCGGGCCCCGCGCTCACGACCTCCGGTTCGATCG
CGCGGATCAAGATCGACGGACTGATCCGCAGCAACTCCGAACGGGTCGAGGCGCTGGAGCGGCTGGAGAAGTCGAACAGC
GCCGCGGTGATCGTCCACATCAATTCGCCGGGCGGCACCACCGCCGGCTCGGAAGAACTCTACACCGCGCTGATGCGGCT
GAAGGCCAAGAAGCCGATGGTGGTCGTGGTCGAAGGCCTGGCGGCGTCGGGCGGCTACATCGCCGCGCTCGCCTCCGATC
ACATCATCGCCCAGCAGAGTTCGCTGGTCGGCTCGATCGGCGTGCTGTTTCAGTATCCGAACGTCTCCGAACTGATGAAG
ACGGTCGGCGTCAAGGTCGAGGAGGTGAAGTCGTCGCCGCTGAAGGCCGCGCCGAACGGCTTCGAGCCGACCAGCCCCGA
GGCGCGCGCCGCGCTGGAATCGCTGGTGCGGGATTCCTACGCGTGGTTTCGCGATCTGGTGAAGCAGCGCCGCGCCATGG
ACGACGATCAGCTCGGCATCGTCGCCGACGGCCGCGTCTTCACCGGCCGACAGGCGGTGGGTCTGAAGCTGATCGATCAG
CTCGGCGACGAGAAGACCGCGGTGGCGTGGCTCGAAACCGAGAAGCGGGTCAAGAGCGGCCTGCCGGTGCGCGACTTCAA
GCTGTCGCCGCAATTCGGCGACCTGACATTTCTGCGGGCCGCAGCGTCGATCACGCTCGATGCGCTCGGCCTCGGCGTGA
TCGCCCGACAGGTGGAGCAGTCCGGCGCGATGCATCTGGGCCTCGACGGCATGCTCGCTTTGTGGGTGCCGGGTAGCACC
CGCTGA

Upstream 100 bases:

>100_bases
CTTGTTTTCTTCAAATTGGGGCGCAATTGATGTATTCCCGTCACGGGAAATCAACGATCCCCTTTGAAAACCGTGAATAG
TCTTCCGCCGGAGAATCTCC

Downstream 100 bases:

>100_bases
CGACGATCGGTGGCCGGCGTCGCACGGCTTCGACCCCGGCGGATGAGCGCGAGCCGCATCGTGCGAGCACCGCCGGACGA
CTTTGTCCAGCCCGCCTCAT

Product: signal peptide peptidase SppA, 36K type

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MSLDSDVIVDRRRIRRKLTFWRVAAVLVAIGAVIAVGAMVSPAGPALTTSGSIARIKIDGLIRSNSERVEALERLEKSNS
AAVIVHINSPGGTTAGSEELYTALMRLKAKKPMVVVVEGLAASGGYIAALASDHIIAQQSSLVGSIGVLFQYPNVSELMK
TVGVKVEEVKSSPLKAAPNGFEPTSPEARAALESLVRDSYAWFRDLVKQRRAMDDDQLGIVADGRVFTGRQAVGLKLIDQ
LGDEKTAVAWLETEKRVKSGLPVRDFKLSPQFGDLTFLRAAASITLDALGLGVIARQVEQSGAMHLGLDGMLALWVPGST
R

Sequences:

>Translated_321_residues
MSLDSDVIVDRRRIRRKLTFWRVAAVLVAIGAVIAVGAMVSPAGPALTTSGSIARIKIDGLIRSNSERVEALERLEKSNS
AAVIVHINSPGGTTAGSEELYTALMRLKAKKPMVVVVEGLAASGGYIAALASDHIIAQQSSLVGSIGVLFQYPNVSELMK
TVGVKVEEVKSSPLKAAPNGFEPTSPEARAALESLVRDSYAWFRDLVKQRRAMDDDQLGIVADGRVFTGRQAVGLKLIDQ
LGDEKTAVAWLETEKRVKSGLPVRDFKLSPQFGDLTFLRAAASITLDALGLGVIARQVEQSGAMHLGLDGMLALWVPGST
R
>Mature_320_residues
SLDSDVIVDRRRIRRKLTFWRVAAVLVAIGAVIAVGAMVSPAGPALTTSGSIARIKIDGLIRSNSERVEALERLEKSNSA
AVIVHINSPGGTTAGSEELYTALMRLKAKKPMVVVVEGLAASGGYIAALASDHIIAQQSSLVGSIGVLFQYPNVSELMKT
VGVKVEEVKSSPLKAAPNGFEPTSPEARAALESLVRDSYAWFRDLVKQRRAMDDDQLGIVADGRVFTGRQAVGLKLIDQL
GDEKTAVAWLETEKRVKSGLPVRDFKLSPQFGDLTFLRAAASITLDALGLGVIARQVEQSGAMHLGLDGMLALWVPGSTR

Specific function: Digestion Of The Cleaved Signal Peptides. This Activity Is Necessary To Maintain Proper Secretion Of Mature Proteins Across The Membrane. [C]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=175, Percent_Identity=34.8571428571429, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787527, Length=162, Percent_Identity=32.0987654320988, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 34271; Mature: 34140

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDSDVIVDRRRIRRKLTFWRVAAVLVAIGAVIAVGAMVSPAGPALTTSGSIARIKIDG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEEC
LIRSNSERVEALERLEKSNSAAVIVHINSPGGTTAGSEELYTALMRLKAKKPMVVVVEGL
EECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECC
AASGGYIAALASDHIIAQQSSLVGSIGVLFQYPNVSELMKTVGVKVEEVKSSPLKAAPNG
CCCCCEEEEECCCCHHHHHHHHHHCCHHEEECCCHHHHHHHHCCCHHHHCCCCCCCCCCC
FEPTSPEARAALESLVRDSYAWFRDLVKQRRAMDDDQLGIVADGRVFTGRQAVGLKLIDQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEECCCHHHHHHHHHH
LGDEKTAVAWLETEKRVKSGLPVRDFKLSPQFGDLTFLRAAASITLDALGLGVIARQVEQ
CCCCCCEEEHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMHLGLDGMLALWVPGSTR
CCCEEECCCCEEEEECCCCCC
>Mature Secondary Structure 
SLDSDVIVDRRRIRRKLTFWRVAAVLVAIGAVIAVGAMVSPAGPALTTSGSIARIKIDG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEEEEEEC
LIRSNSERVEALERLEKSNSAAVIVHINSPGGTTAGSEELYTALMRLKAKKPMVVVVEGL
EECCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECC
AASGGYIAALASDHIIAQQSSLVGSIGVLFQYPNVSELMKTVGVKVEEVKSSPLKAAPNG
CCCCCEEEEECCCCHHHHHHHHHHCCHHEEECCCHHHHHHHHCCCHHHHCCCCCCCCCCC
FEPTSPEARAALESLVRDSYAWFRDLVKQRRAMDDDQLGIVADGRVFTGRQAVGLKLIDQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCEEECCCHHHHHHHHHH
LGDEKTAVAWLETEKRVKSGLPVRDFKLSPQFGDLTFLRAAASITLDALGLGVIARQVEQ
CCCCCCEEEHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAMHLGLDGMLALWVPGSTR
CCCEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]