Click here to switch to the map view.

The map label for this gene is trpF

Identifier: 86747762

GI number: 86747762

Start: 721615

End: 722271

Strand: Reverse

Name: trpF

Synonym: RPB_0636

Alternate gene names: 86747762

Gene position: 722271-721615 (Counterclockwise)

Preceding gene: 86747763

Following gene: 86747761

Centisome position: 13.55

GC content: 70.78

Gene sequence:

>657_bases
ATGTCCGTCGTCGTCAAAATATGCGGCCTGTCGACCCGCGACACGCTCGAAGCGGCGGTCGCGGCCGGCGCCGACATGGT
CGGCTTCGTGTTCTTTCCGGCCTCGCCGCGTCATGTCGGGTTCGACCTCGCGCGCGCACTCGGCGACCAGGTCGGCAGCC
GCGCCGCGAAAGTCGCGCTGACGGTCGACGCCAGCGACGCGCTGCTGCGCGACGTCGTCGACGCGCTCGCGCCGGATCTG
CTGCAACTTCACGGCAAGGAAAGCCCCGAGCGGGTTCGCGCGATCAGGCAGACATTCGGGCTGCCGGTGATGAAGGCCGT
GGCGGTGGCCACCGCTGATGATCTGGCGGCGCTGCCCGCTTACGCCGCCGCAGCCGATCGCATCCTGTTCGACGCGCGGC
CGCCGAAGGACGCGCTCCGCCCCGGCGGTCTCGGCGTGCCGTTCGACTGGGCGCTGCTGAGCGGCCTCGCACTGCCGGTC
CCGTACATGGTGTCCGGCGGCATCACGCCCGGCAATGTCGCCGAGGCGCTGCGGGTGACGCGCGCCGGCGGCATCGACGT
GTCCTCCGGCGTCGAGACGGCGCCCGGCGTCAAAGATTCCGAATTGATCCGCAGCTTCATCCGCGCCACGCGCGCCAGCG
AAGAGACGATGACCTGA

Upstream 100 bases:

>100_bases
GTTCCGCCTCCGGCAGGCGCGACAAGGCCAGCGCGGCGCTGTAGAACACCGTCGTGTTGCCGGCTCCCCTCGTCGCCGGC
CGAACCCCGATCGCGAACCC

Downstream 100 bases:

>100_bases
CATGAACCAGATCCTGCCGAACTCGTTTCGATCCGGTCCCGACGAGCGCGGGCATTTCGGCATCTTCGGCGGCCGCTTCG
TCGCCGAGACGCTGATGCCG

Product: N-(5'-phosphoribosyl)anthranilate isomerase

Products: NA

Alternate protein names: PRAI

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MSVVVKICGLSTRDTLEAAVAAGADMVGFVFFPASPRHVGFDLARALGDQVGSRAAKVALTVDASDALLRDVVDALAPDL
LQLHGKESPERVRAIRQTFGLPVMKAVAVATADDLAALPAYAAAADRILFDARPPKDALRPGGLGVPFDWALLSGLALPV
PYMVSGGITPGNVAEALRVTRAGGIDVSSGVETAPGVKDSELIRSFIRATRASEETMT

Sequences:

>Translated_218_residues
MSVVVKICGLSTRDTLEAAVAAGADMVGFVFFPASPRHVGFDLARALGDQVGSRAAKVALTVDASDALLRDVVDALAPDL
LQLHGKESPERVRAIRQTFGLPVMKAVAVATADDLAALPAYAAAADRILFDARPPKDALRPGGLGVPFDWALLSGLALPV
PYMVSGGITPGNVAEALRVTRAGGIDVSSGVETAPGVKDSELIRSFIRATRASEETMT
>Mature_217_residues
SVVVKICGLSTRDTLEAAVAAGADMVGFVFFPASPRHVGFDLARALGDQVGSRAAKVALTVDASDALLRDVVDALAPDLL
QLHGKESPERVRAIRQTFGLPVMKAVAVATADDLAALPAYAAAADRILFDARPPKDALRPGGLGVPFDWALLSGLALPVP
YMVSGGITPGNVAEALRVTRAGGIDVSSGVETAPGVKDSELIRSFIRATRASEETMT

Specific function: Bifunctional Enzyme That Catalyzes Two Sequential Steps Of Tryptophan Biosynthetic Pathway. The First Reaction Is Catalyzed By The Isomerase, Coded By The Trpf Domain; The Second Reaction Is Catalyzed By The Synthase, Coded By The Trpc Domain. [C]

COG id: COG0135

COG function: function code E; Phosphoribosylanthranilate isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpF family

Homologues:

Organism=Escherichia coli, GI87081863, Length=210, Percent_Identity=30.952380952381, Blast_Score=78, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320210, Length=222, Percent_Identity=29.2792792792793, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRPF_RHOP2 (Q2J2G3)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484258.1
- ProteinModelPortal:   Q2J2G3
- SMR:   Q2J2G3
- STRING:   Q2J2G3
- GeneID:   3908329
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0636
- eggNOG:   COG0135
- HOGENOM:   HBG554803
- OMA:   SKICGIT
- ProtClustDB:   PRK01222
- BioCyc:   RPAL316058:RPB_0636-MONOMER
- HAMAP:   MF_00135_B
- InterPro:   IPR013785
- InterPro:   IPR001240
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF00697 PRAI; SSF51366 RibP_bind_barrel

EC number: =5.3.1.24

Molecular weight: Translated: 22589; Mature: 22458

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVVKICGLSTRDTLEAAVAAGADMVGFVFFPASPRHVGFDLARALGDQVGSRAAKVAL
CCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHEEEEE
TVDASDALLRDVVDALAPDLLQLHGKESPERVRAIRQTFGLPVMKAVAVATADDLAALPA
EECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
YAAAADRILFDARPPKDALRPGGLGVPFDWALLSGLALPVPYMVSGGITPGNVAEALRVT
HHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHCCHHCCCCHHHCCCCCCHHHHHHHHHH
RAGGIDVSSGVETAPGVKDSELIRSFIRATRASEETMT
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCC
>Mature Secondary Structure 
SVVVKICGLSTRDTLEAAVAAGADMVGFVFFPASPRHVGFDLARALGDQVGSRAAKVAL
CEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCHHEEEEE
TVDASDALLRDVVDALAPDLLQLHGKESPERVRAIRQTFGLPVMKAVAVATADDLAALPA
EECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
YAAAADRILFDARPPKDALRPGGLGVPFDWALLSGLALPVPYMVSGGITPGNVAEALRVT
HHHHHHHHHCCCCCCHHHCCCCCCCCCHHHHHHCCHHCCCCHHHCCCCCCHHHHHHHHHH
RAGGIDVSSGVETAPGVKDSELIRSFIRATRASEETMT
HCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA