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The map label for this gene is trpA

Identifier: 86747760

GI number: 86747760

Start: 719563

End: 720399

Strand: Reverse

Name: trpA

Synonym: RPB_0634

Alternate gene names: 86747760

Gene position: 720399-719563 (Counterclockwise)

Preceding gene: 86747761

Following gene: 86747759

Centisome position: 13.51

GC content: 69.3

Gene sequence:

>837_bases
ATGACTACGCGTATCGACATCCGCTTCGCCCAGCTCAAGCGCGAAGGCCGCCCGGCCTTCGTCACCTTCGTGATGGCGGG
CGATCCCGACCTCGCCACCTCGCTGCAGCTGCTCAAGGCGCTACCCACGGCCGGCGCGGACATCATCGAGATCGGCATGC
CGTTCACCGACCCGATGGCCGACGGACCGGCGATCCAGGCCGCCGGCTTGCGCGCGCTGCACGGCGGCACCACGCTGCAC
AAGACGCTCGAGCTGGTGGGCGACTTCCGCAAGGACGACGCCACTACGCCAGTGGTGCTGATGGGCTACTACAATCCGAT
CTACATCTACGGCGTCGACGCGTTTCTCGCCGACGCCAAGGCGGCCGGGGTCGACGGGCTGATCATCGTCGATCTGCCGC
CCGAGGAAGACGCCGAGCTGTGCCTGCCGGCGATGAAGGCCGGGCTGAATTTCATCCGGCTGGCGACGCCCACCACCGAC
GAAAAGCGGTTGCCGGCGGTGCTCGCCAACACCTCGGGCTTCGTCTATTACGTGTCGATCACCGGCATCACCGGCTCGGC
TTCGGCGGATTCGGTCGCGGTCGGCGACGCGGTGGCGCGGATCAAGCGCCACACCGACCTGCCGGTCTGCGTCGGCTTCG
GCATCCGCACCCCCGAGGGGGCGCGCGCGATCGCCGCACAGGCCGACGGCGCGGTGGTCGGATCGGCCCTGATCGACGCG
CTGCAGAAGACGCTCGACACCGACAATCGCGCCACTCAGGCGACCGTCGGGGCCGTCGCGGATCTGGTCGCCTCGCTTGC
GGCCGGGGTGCGCGGCGCCCAACAGGCTGCCGAATAG

Upstream 100 bases:

>100_bases
CCCCGCCCCTGCCGAAGGATCATGTGATGGTGCTCAACATGTCGGGCCGCGGCGACAAGGATCTCGCTTCGGTCGCCGAA
CATCTCGGGGGCCAGTTCTG

Downstream 100 bases:

>100_bases
GCAGTCATCAATTCGGGCCATGGTCTGGACGGAGCCGTTGTCCGCCGCCATATCCCGACAGTGCAATGACCCTGGCGACG
CCCTTCCGGGGGCGACGCGG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTTRIDIRFAQLKREGRPAFVTFVMAGDPDLATSLQLLKALPTAGADIIEIGMPFTDPMADGPAIQAAGLRALHGGTTLH
KTLELVGDFRKDDATTPVVLMGYYNPIYIYGVDAFLADAKAAGVDGLIIVDLPPEEDAELCLPAMKAGLNFIRLATPTTD
EKRLPAVLANTSGFVYYVSITGITGSASADSVAVGDAVARIKRHTDLPVCVGFGIRTPEGARAIAAQADGAVVGSALIDA
LQKTLDTDNRATQATVGAVADLVASLAAGVRGAQQAAE

Sequences:

>Translated_278_residues
MTTRIDIRFAQLKREGRPAFVTFVMAGDPDLATSLQLLKALPTAGADIIEIGMPFTDPMADGPAIQAAGLRALHGGTTLH
KTLELVGDFRKDDATTPVVLMGYYNPIYIYGVDAFLADAKAAGVDGLIIVDLPPEEDAELCLPAMKAGLNFIRLATPTTD
EKRLPAVLANTSGFVYYVSITGITGSASADSVAVGDAVARIKRHTDLPVCVGFGIRTPEGARAIAAQADGAVVGSALIDA
LQKTLDTDNRATQATVGAVADLVASLAAGVRGAQQAAE
>Mature_277_residues
TTRIDIRFAQLKREGRPAFVTFVMAGDPDLATSLQLLKALPTAGADIIEIGMPFTDPMADGPAIQAAGLRALHGGTTLHK
TLELVGDFRKDDATTPVVLMGYYNPIYIYGVDAFLADAKAAGVDGLIIVDLPPEEDAELCLPAMKAGLNFIRLATPTTDE
KRLPAVLANTSGFVYYVSITGITGSASADSVAVGDAVARIKRHTDLPVCVGFGIRTPEGARAIAAQADGAVVGSALIDAL
QKTLDTDNRATQATVGAVADLVASLAAGVRGAQQAAE

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=248, Percent_Identity=33.0645161290323, Blast_Score=151, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6321412, Length=239, Percent_Identity=42.6778242677824, Blast_Score=199, Evalue=4e-52,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_RHOP2 (Q2J2G5)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484256.1
- HSSP:   P00929
- ProteinModelPortal:   Q2J2G5
- SMR:   Q2J2G5
- STRING:   Q2J2G5
- GeneID:   3908327
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0634
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   RPAL316058:RPB_0634-MONOMER
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28820; Mature: 28689

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 50-50 ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRIDIRFAQLKREGRPAFVTFVMAGDPDLATSLQLLKALPTAGADIIEIGMPFTDPMA
CCEEEEEEEHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
DGPAIQAAGLRALHGGTTLHKTLELVGDFRKDDATTPVVLMGYYNPIYIYGVDAFLADAK
CCCCCHHCCEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEHHHHHHHHH
AAGVDGLIIVDLPPEEDAELCLPAMKAGLNFIRLATPTTDEKRLPAVLANTSGFVYYVSI
HCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCEEEEEEE
TGITGSASADSVAVGDAVARIKRHTDLPVCVGFGIRTPEGARAIAAQADGAVVGSALIDA
ECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHH
LQKTLDTDNRATQATVGAVADLVASLAAGVRGAQQAAE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
TTRIDIRFAQLKREGRPAFVTFVMAGDPDLATSLQLLKALPTAGADIIEIGMPFTDPMA
CEEEEEEEHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
DGPAIQAAGLRALHGGTTLHKTLELVGDFRKDDATTPVVLMGYYNPIYIYGVDAFLADAK
CCCCCHHCCEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEHHHHHHHHH
AAGVDGLIIVDLPPEEDAELCLPAMKAGLNFIRLATPTTDEKRLPAVLANTSGFVYYVSI
HCCCCCEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEECCCCEEEEEEE
TGITGSASADSVAVGDAVARIKRHTDLPVCVGFGIRTPEGARAIAAQADGAVVGSALIDA
ECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEECCCCHHHHHHHHHH
LQKTLDTDNRATQATVGAVADLVASLAAGVRGAQQAAE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA