Click here to switch to the map view.

The map label for this gene is ubiE

Identifier: 86747746

GI number: 86747746

Start: 699169

End: 699930

Strand: Direct

Name: ubiE

Synonym: RPB_0620

Alternate gene names: 86747746

Gene position: 699169-699930 (Clockwise)

Preceding gene: 86747744

Following gene: 86747747

Centisome position: 13.11

GC content: 64.83

Gene sequence:

>762_bases
ATGACCGAGCCGGGCCAGACCACGCATTTCGGCTATCGCGACGTTCCGCTGCAGGACAAGCAGACGCTGGTCAACGACGT
GTTCCACAGCGTCGCCAGCCGCTACGACCTGATGAACGACCTGATGTCGGGCGGCATGCATCGGTTGTGGAAGGACGTGA
TGATCACGACCTTGAACCCGCCGCGCGACGACCAGCCGTTCCGCCTGCTCGACGTCGCCGGCGGCACCGGCGACATCTCG
TTTCGCGCGGCGAAGGCGTCCGGCGCGGGCTTCCAGGCCACCGTCTGCGACATCAACACCGACATGCTCGAGGTCGGCCG
CCAGCGCGCCGTCGAGCGCCATCTCGACGGCCAGGTCGAATTCGTCGAGGGCAATGCCGAGGCGCTGCAGTTCCCCGACA
AGAGCTACGACGCCTATACGATCGCCTTCGGCATCCGCAACGTGCCGCGGATCGATCTGGCGCTGAAGGAAGCCCATCGG
GTGCTGAAGCCCGGCAGCCGCTTCCTGTGCCTCGAATTCTCCAGCGTCGACGTGCCGGGCCTCGCCAAGATCTACGACCT
GTTCTCGTTCAAGGTGATCCCGCAGATTGGCCGCGTCGTCACCGGCGACGCCGAGAGCTATCAATATCTGGTCGAATCGA
TCCGCAAATTTCCCAAGCCCGCCGATTTCGGCGAGATGATGCGCGAGGCCGGCTTCGCGCGCGTCAACTGGCAGGTGTTG
TCCGGCGGCATCGTCGCGCTGCATTCGGGCTGGCGTTTGTGA

Upstream 100 bases:

>100_bases
CTGCCGCCCCGGCGCGCTATATGGTCCGCCGCGGAGCGGCATTTCGTGTTCCGGGCGGCATCGCGCCCTGGAGAACCGTC
CGTATGCAGGAGTTGAAGGC

Downstream 100 bases:

>100_bases
TCGCAGCACTCACTCATGTGGCGCGGCTCGCCCGCGCCGCCTTCGTGTTCGCGCGCGAGGGCGTGTTCGGCGTGGTCGAT
CCGTCGCTGATTCCGCCGGC

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTEPGQTTHFGYRDVPLQDKQTLVNDVFHSVASRYDLMNDLMSGGMHRLWKDVMITTLNPPRDDQPFRLLDVAGGTGDIS
FRAAKASGAGFQATVCDINTDMLEVGRQRAVERHLDGQVEFVEGNAEALQFPDKSYDAYTIAFGIRNVPRIDLALKEAHR
VLKPGSRFLCLEFSSVDVPGLAKIYDLFSFKVIPQIGRVVTGDAESYQYLVESIRKFPKPADFGEMMREAGFARVNWQVL
SGGIVALHSGWRL

Sequences:

>Translated_253_residues
MTEPGQTTHFGYRDVPLQDKQTLVNDVFHSVASRYDLMNDLMSGGMHRLWKDVMITTLNPPRDDQPFRLLDVAGGTGDIS
FRAAKASGAGFQATVCDINTDMLEVGRQRAVERHLDGQVEFVEGNAEALQFPDKSYDAYTIAFGIRNVPRIDLALKEAHR
VLKPGSRFLCLEFSSVDVPGLAKIYDLFSFKVIPQIGRVVTGDAESYQYLVESIRKFPKPADFGEMMREAGFARVNWQVL
SGGIVALHSGWRL
>Mature_252_residues
TEPGQTTHFGYRDVPLQDKQTLVNDVFHSVASRYDLMNDLMSGGMHRLWKDVMITTLNPPRDDQPFRLLDVAGGTGDISF
RAAKASGAGFQATVCDINTDMLEVGRQRAVERHLDGQVEFVEGNAEALQFPDKSYDAYTIAFGIRNVPRIDLALKEAHRV
LKPGSRFLCLEFSSVDVPGLAKIYDLFSFKVIPQIGRVVTGDAESYQYLVESIRKFPKPADFGEMMREAGFARVNWQVLS
GGIVALHSGWRL

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=279, Percent_Identity=47.6702508960573, Blast_Score=259, Evalue=2e-69,
Organism=Escherichia coli, GI48994983, Length=248, Percent_Identity=47.1774193548387, Blast_Score=234, Evalue=5e-63,
Organism=Caenorhabditis elegans, GI17557039, Length=254, Percent_Identity=46.8503937007874, Blast_Score=227, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6323526, Length=264, Percent_Identity=44.6969696969697, Blast_Score=207, Evalue=1e-54,
Organism=Drosophila melanogaster, GI24641702, Length=261, Percent_Identity=46.360153256705, Blast_Score=236, Evalue=9e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_RHOP2 (Q2J2H9)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484242.1
- ProteinModelPortal:   Q2J2H9
- SMR:   Q2J2H9
- STRING:   Q2J2H9
- GeneID:   3908313
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0620
- eggNOG:   COG2226
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- ProtClustDB:   PRK00216
- BioCyc:   RPAL316058:RPB_0620-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 28247; Mature: 28115

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPGQTTHFGYRDVPLQDKQTLVNDVFHSVASRYDLMNDLMSGGMHRLWKDVMITTLNP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEECCC
PRDDQPFRLLDVAGGTGDISFRAAKASGAGFQATVCDINTDMLEVGRQRAVERHLDGQVE
CCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEE
FVEGNAEALQFPDKSYDAYTIAFGIRNVPRIDLALKEAHRVLKPGSRFLCLEFSSVDVPG
EEECCCCEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
LAKIYDLFSFKVIPQIGRVVTGDAESYQYLVESIRKFPKPADFGEMMREAGFARVNWQVL
HHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEE
SGGIVALHSGWRL
CCCEEEECCCCCC
>Mature Secondary Structure 
TEPGQTTHFGYRDVPLQDKQTLVNDVFHSVASRYDLMNDLMSGGMHRLWKDVMITTLNP
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEEECCC
PRDDQPFRLLDVAGGTGDISFRAAKASGAGFQATVCDINTDMLEVGRQRAVERHLDGQVE
CCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEE
FVEGNAEALQFPDKSYDAYTIAFGIRNVPRIDLALKEAHRVLKPGSRFLCLEFSSVDVPG
EEECCCCEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCH
LAKIYDLFSFKVIPQIGRVVTGDAESYQYLVESIRKFPKPADFGEMMREAGFARVNWQVL
HHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEE
SGGIVALHSGWRL
CCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA