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The map label for this gene is cobT [H]

Identifier: 86747669

GI number: 86747669

Start: 607462

End: 609357

Strand: Direct

Name: cobT [H]

Synonym: RPB_0543

Alternate gene names: 86747669

Gene position: 607462-609357 (Clockwise)

Preceding gene: 86747668

Following gene: 86747670

Centisome position: 11.39

GC content: 68.04

Gene sequence:

>1896_bases
ATGACCACATCCAACACCAAATTCCGCACCGGATCGAAGGAAGCGCCGACCGAGCCGTTCAAGCGGGCGGTGACGTCGTG
CCTGCGGGCGATCGCCAAGACGCCGGAGCTCGAAGTCAGCTTCGCGGCGGAGCGCCCCGGCCTGTCGCCCGGCAAGGCGC
GGCTGCCGGAGCCGGCGCGAAAGATGAGCAAGCGCGACGCCGCGATCGTGCGCGGCCATGCCGATTCGATCGCGCTGAAG
CTCGCTTGCCACGATCCGAAAGTGCATCGCAAGCTGATGCCGGGCAATCCGCAAGCGCGCGGCGTATTCGAAGCCGTCGA
GCAGGCCCGCGTCGAAGCGCTCGGCGCTCGGCGGATGGCGGGGGTCGCCAAGAACCTCACCGCGATGCTCGACGATCACT
TCCACCGCGGTAAGTTCGACGAGATCACCGACCGTGCCGACGCGCCGTTGTCGGACGCGCTGGCGATGCTGGTGCGCGAG
CGCCTCACCGGGCTGGCGCCGCCGGCGGCGGCGAAGAAGCTGGTCGACCTGTGGCGGCCGGTGCTGGAAGACAAGATCGG
GCCGAAGCTCGACGAGCTCGAACGCTTCGCCGAGAACCAGGCGAAGTTCGGCGACGCCCTCCACGACCTGCTCGACGTGC
TCGAACTCGGCGACGATCGCGACGCCGATTCGGACGAGGAAGAGAACCAGGACGACAAGCAGGAAGGCGAGAACGATCAG
TCCGGCGCCGAAGGCACGCCGGAGAGCGAAGCCGCCCAGGAGATGAGCGCCGACCAGGCCGAGGCGACCAGCGACGACCT
CAGCGACAGCGCGATGGAAAGCGCGCAGGCTTCGGCCTCCGATGCCTTCGACGATTCCGAGACCGGCGAGGACGACACGC
CGGGCGAGGCGACGCGGCCGAACAATCGCGGCGCCAACGAGCCGCGCGGGCCGGAATATCACGCCTTCGCGCCGAAATTC
GACGAGGTCGTCGCCGCCGAGGACCTGTGCGACCACGACGAGCTGGAGCGGCTGCGCAGCTATCTCGACAAGCAGCTCGC
GCATCTGCAGGGCATCGTCGCGCGGCTCGCCAACCGGCTGCAGCGCCGCCTGATGGCGCAGCAGAACCGCGCCTGGGATT
TCGACCTCGAGGAAGGCATTCTCGATCCGGCCCGGCTGTCGCGTGTCGTCACCGACCCGTTCCATCCGCTGTCGTTCATG
AGCGAGAAGGAAGCCACCTTCCGCGACACCGTGGTGACGCTGCTGCTGGACAATTCCGGCTCGATGCGCGGCCGCCCGAT
CACCGTGGCGGCGACCTGCGCCGACATTCTGGCGCGGACGCTGGAGCGCTGCGGCGTCAAGGTCGAGATTCTCGGCTTCA
CGACGCGCGCCTGGAAGGGCGGTCAATCGCGTGAGGCGTGGCTCGCGGCCGGCAAGCCGGCGTCGCCGGGCCGGCTCAAC
GATCTGCGCCACATCATCTACAAATCCGCCGACGCGCCGTGGCGCCGGGCCCGCAGGAATCTCGGGCTGATGATGCGCGA
GGGGCTGCTCAAGGAGAACATCGACGGCGAGGCGCTGGACTGGGCGCACAAGCGGCTGCTCGGCAGAAGCGAACAGCGCA
AGATCCTGATGATGATTTCTGACGGCGCGCCGGTCGACGATTCGACGCTGTCGGTCAATCCCGGCAATTATCTCGAGCGG
CATCTGCGCCACATCATCGAGGAGATCGAGACCCGCTCGCCGGTCGAGCTGATCGCGATCGGCATCGGCCACGACGTCAC
CCGCTATTATCGTCGCGCGGTGACCATCGTCGACGCCGAGGAGCTCGGCGGCGCGATCACCGAGAAGCTCGCCGAACTGT
TCAGCGAAACCGCGGACGTGCCCGCGCCGGGTCGCCGTCGTCGGCTGCATTCGTGA

Upstream 100 bases:

>100_bases
AAGCGTCTTCGAGTTGACCTGTCCGTATGATTGGGATGCGATCCAATCTCGTGCCGTTGAGTTTGAAGACATCCGCTCAC
CGGAAGAGTAATTTCTCAGC

Downstream 100 bases:

>100_bases
TCGCGCGCGAAGCGCGATGACACGACCGACCGCGCACTCGGCTGAGCTGAAAGTGCACAGCGCGAGCAAGGGAGCCGGCG
CGCTCCCTCTCCCCTCTCCG

Product: cobalt chelatase, pCobT subunit

Products: NA

Alternate protein names: Hydrogenobyrinic acid a,c-diamide cobaltochelatase subunit CobT [H]

Number of amino acids: Translated: 631; Mature: 630

Protein sequence:

>631_residues
MTTSNTKFRTGSKEAPTEPFKRAVTSCLRAIAKTPELEVSFAAERPGLSPGKARLPEPARKMSKRDAAIVRGHADSIALK
LACHDPKVHRKLMPGNPQARGVFEAVEQARVEALGARRMAGVAKNLTAMLDDHFHRGKFDEITDRADAPLSDALAMLVRE
RLTGLAPPAAAKKLVDLWRPVLEDKIGPKLDELERFAENQAKFGDALHDLLDVLELGDDRDADSDEEENQDDKQEGENDQ
SGAEGTPESEAAQEMSADQAEATSDDLSDSAMESAQASASDAFDDSETGEDDTPGEATRPNNRGANEPRGPEYHAFAPKF
DEVVAAEDLCDHDELERLRSYLDKQLAHLQGIVARLANRLQRRLMAQQNRAWDFDLEEGILDPARLSRVVTDPFHPLSFM
SEKEATFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPASPGRLN
DLRHIIYKSADAPWRRARRNLGLMMREGLLKENIDGEALDWAHKRLLGRSEQRKILMMISDGAPVDDSTLSVNPGNYLER
HLRHIIEEIETRSPVELIAIGIGHDVTRYYRRAVTIVDAEELGGAITEKLAELFSETADVPAPGRRRRLHS

Sequences:

>Translated_631_residues
MTTSNTKFRTGSKEAPTEPFKRAVTSCLRAIAKTPELEVSFAAERPGLSPGKARLPEPARKMSKRDAAIVRGHADSIALK
LACHDPKVHRKLMPGNPQARGVFEAVEQARVEALGARRMAGVAKNLTAMLDDHFHRGKFDEITDRADAPLSDALAMLVRE
RLTGLAPPAAAKKLVDLWRPVLEDKIGPKLDELERFAENQAKFGDALHDLLDVLELGDDRDADSDEEENQDDKQEGENDQ
SGAEGTPESEAAQEMSADQAEATSDDLSDSAMESAQASASDAFDDSETGEDDTPGEATRPNNRGANEPRGPEYHAFAPKF
DEVVAAEDLCDHDELERLRSYLDKQLAHLQGIVARLANRLQRRLMAQQNRAWDFDLEEGILDPARLSRVVTDPFHPLSFM
SEKEATFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPASPGRLN
DLRHIIYKSADAPWRRARRNLGLMMREGLLKENIDGEALDWAHKRLLGRSEQRKILMMISDGAPVDDSTLSVNPGNYLER
HLRHIIEEIETRSPVELIAIGIGHDVTRYYRRAVTIVDAEELGGAITEKLAELFSETADVPAPGRRRRLHS
>Mature_630_residues
TTSNTKFRTGSKEAPTEPFKRAVTSCLRAIAKTPELEVSFAAERPGLSPGKARLPEPARKMSKRDAAIVRGHADSIALKL
ACHDPKVHRKLMPGNPQARGVFEAVEQARVEALGARRMAGVAKNLTAMLDDHFHRGKFDEITDRADAPLSDALAMLVRER
LTGLAPPAAAKKLVDLWRPVLEDKIGPKLDELERFAENQAKFGDALHDLLDVLELGDDRDADSDEEENQDDKQEGENDQS
GAEGTPESEAAQEMSADQAEATSDDLSDSAMESAQASASDAFDDSETGEDDTPGEATRPNNRGANEPRGPEYHAFAPKFD
EVVAAEDLCDHDELERLRSYLDKQLAHLQGIVARLANRLQRRLMAQQNRAWDFDLEEGILDPARLSRVVTDPFHPLSFMS
EKEATFRDTVVTLLLDNSGSMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPASPGRLND
LRHIIYKSADAPWRRARRNLGLMMREGLLKENIDGEALDWAHKRLLGRSEQRKILMMISDGAPVDDSTLSVNPGNYLERH
LRHIIEEIETRSPVELIAIGIGHDVTRYYRRAVTIVDAEELGGAITEKLAELFSETADVPAPGRRRRLHS

Specific function: Catalyzes cobalt insertion in the corrin ring [H]

COG id: COG4547

COG function: function code H; Cobalamin biosynthesis protein CobT (nicotinate-mononucleotide:5, 6-dimethylbenzimidazole phosphoribosyltransferase)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 VWFA domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006538
- InterPro:   IPR002035 [H]

Pfam domain/function: PF06213 CobT [H]

EC number: =6.6.1.2 [H]

Molecular weight: Translated: 69751; Mature: 69620

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50234 VWFA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSNTKFRTGSKEAPTEPFKRAVTSCLRAIAKTPELEVSFAAERPGLSPGKARLPEPAR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHH
KMSKRDAAIVRGHADSIALKLACHDPKVHRKLMPGNPQARGVFEAVEQARVEALGARRMA
HHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GVAKNLTAMLDDHFHRGKFDEITDRADAPLSDALAMLVRERLTGLAPPAAAKKLVDLWRP
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VLEDKIGPKLDELERFAENQAKFGDALHDLLDVLELGDDRDADSDEEENQDDKQEGENDQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCC
SGAEGTPESEAAQEMSADQAEATSDDLSDSAMESAQASASDAFDDSETGEDDTPGEATRP
CCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
NNRGANEPRGPEYHAFAPKFDEVVAAEDLCDHDELERLRSYLDKQLAHLQGIVARLANRL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRRLMAQQNRAWDFDLEEGILDPARLSRVVTDPFHPLSFMSEKEATFRDTVVTLLLDNSG
HHHHHHHHCCCCCCCHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEECCCC
SMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPASPGRLN
CCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCHHEECCCCCCCCHHH
DLRHIIYKSADAPWRRARRNLGLMMREGLLKENIDGEALDWAHKRLLGRSEQRKILMMIS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEE
DGAPVDDSTLSVNPGNYLERHLRHIIEEIETRSPVELIAIGIGHDVTRYYRRAVTIVDAE
CCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHEECHH
ELGGAITEKLAELFSETADVPAPGRRRRLHS
HHCHHHHHHHHHHHHHHCCCCCCCHHCCCCC
>Mature Secondary Structure 
TTSNTKFRTGSKEAPTEPFKRAVTSCLRAIAKTPELEVSFAAERPGLSPGKARLPEPAR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHHH
KMSKRDAAIVRGHADSIALKLACHDPKVHRKLMPGNPQARGVFEAVEQARVEALGARRMA
HHHHHHHHHHCCCCCCEEEEEEECCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
GVAKNLTAMLDDHFHRGKFDEITDRADAPLSDALAMLVRERLTGLAPPAAAKKLVDLWRP
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
VLEDKIGPKLDELERFAENQAKFGDALHDLLDVLELGDDRDADSDEEENQDDKQEGENDQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCCCCC
SGAEGTPESEAAQEMSADQAEATSDDLSDSAMESAQASASDAFDDSETGEDDTPGEATRP
CCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
NNRGANEPRGPEYHAFAPKFDEVVAAEDLCDHDELERLRSYLDKQLAHLQGIVARLANRL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRRLMAQQNRAWDFDLEEGILDPARLSRVVTDPFHPLSFMSEKEATFRDTVVTLLLDNSG
HHHHHHHHCCCCCCCHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEECCCC
SMRGRPITVAATCADILARTLERCGVKVEILGFTTRAWKGGQSREAWLAAGKPASPGRLN
CCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCHHEECCCCCCCCHHH
DLRHIIYKSADAPWRRARRNLGLMMREGLLKENIDGEALDWAHKRLLGRSEQRKILMMIS
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEE
DGAPVDDSTLSVNPGNYLERHLRHIIEEIETRSPVELIAIGIGHDVTRYYRRAVTIVDAE
CCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHEECHH
ELGGAITEKLAELFSETADVPAPGRRRRLHS
HHCHHHHHHHHHHHHHHCCCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1917840 [H]