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The map label for this gene is yfgF [C]

Identifier: 86747637

GI number: 86747637

Start: 572512

End: 574263

Strand: Reverse

Name: yfgF [C]

Synonym: RPB_0511

Alternate gene names: 86747637

Gene position: 574263-572512 (Counterclockwise)

Preceding gene: 86747639

Following gene: 86747635

Centisome position: 10.77

GC content: 68.84

Gene sequence:

>1752_bases
GTGTCGCCAGTCGATCCTACGGCCGCCCAGCCGATCGCGCCGACCGAGTCTCGCGGTCCCGATCCGGCGGAAATTCTGGC
CGCGCTCGGGCAGGCGGCGTTCGTCTGGGAGCTCGCGACCGACGCTCTCGTCTGGACCGGCCCCGCAGCAGGGCTGTTTC
AGGACATTCCCGCCGGAACGCTGGCGACCGGCGCCGGCTACGCCTCGCTGCTCGAGCCGTCGCGCGCGATCCGCGCCGAC
GCGCTGCTCAACGCCTCAGCCGCGGCCGGCGCGCATTGCGTGCCGTACCGGATCGAATACGGCGTCCGCGCCGCGACCTC
GGCGCCGATCATCTGGATCGAGGAAAGCGGCCGCTGGCATCCGGGGCCCGACGGCCGGCCCGCGCGGGTCGAGGGCATCG
TCCGCGTCAACAACGAGCGTCATGCCCGCGACCAGCAATTGCTGAAGCTGTCGCAGAACGATCCGCTGACCGGCGAACTC
AACCGCACCAACCTGGTCGCGGCGCTCGCCGAGGCGATCGAGGAAGCCTCGCGCTTCCGCAACAGCCTCTGCTTCATGCT
GATCGGCATCGACCATCTGTCCCGGATCAACGATTCGTTCGGCTTCGACGTCGCCGACGAAGTGATCTACACCCTTTCGC
AGCGGATCCGCGCACGGCTGCGCGCCGGCGACGAGCTCGGCCGCTTCTCCGGCAACAAGTTCGGCGTGGTGCTGAAGGCC
TGCACCATCGACGACGCCCATGTGGCGGCGGAGCGTTTCCTCGCCGCGATCCGCGACGAGATCGTGCCGACCCGGTCGGG
CCCGGTATCGATCACGGCGTCGATCGGCGCGGTCAGTTCGCCGCGCCACGCCCGTACGGCCGAAGAGGCGATCAACCGCG
CCCAGGAGGCGCTGGCCGCCGCGAAGCATCGGCGCTGCGGCGCGTTTTCGATGTGGCGGCCGAATGTCGAGCGCGACGCC
CAGCGCCGGGTGAATATCCGCGTCACCGACGAGATCGTCACCGCATTGAACGAGCGCCGGATCGTGCTGGCCTACGAGCC
GGTGGTCGACGTCGCGACCCGTGCGCCGGCGTTTCACGAATGCCTGGTCCGGATGCGGCAGAACGACGGCCAGGTGCTGC
TGGCGCCCGACATCGTGCCGGTCGCGGAGCGGCTCGGCCTGATCCGGCTGGTCGATCATCGCGTGCTCGAACTCGTCGTC
GCCGAACTCGCCGCCGCGCCGCAGATGACGCTCAGCCTCAACATCTCACCCGACACCACGATGGACGCCGACTGGTGGGC
CGGGATCGAATCGCTAATGCTGGCGCACCCCGGCGTCGCCGAACGGCTGATCGTCGAGATCACCGAGACGGTCGCGATCC
AGGACATCGAGGAGGTGCGCGGTTTCGTCACGCGGCTGAAAAAATTCGGCGCGAAAATCGCGATCGACGATTTCGGCGCC
GGCTACACCTCGTTCCGCAACCTGCGCAATCTCGGCGTCGATATCGTGAAGATCGACGGCGCCTTCGTGCAGAACATCGC
GCGCTCGGCCGACGACCGCGCCTTCGTCCAGACCCTGATCGATCTGGCGCGCAGGCTCGGTATCAAGACGGTGGCGGAAT
GGGTTCAGGACGACGAGTCGGCGCTGATGCTGCGCGACTGGGGTTGCGATTACATCCAGGGCCGGCTGATCGGGCTGGCG
TCGCAGCACCGCCCATGGGACGGGCCGACCGCGGCGGAGACCGCCGCGGTGCGGGAGCGCGCGGCGAACTAA

Upstream 100 bases:

>100_bases
GGTTTCCAGATTGTTAACGATCTCGCCCTAGCTTGAACAAGGTGCAGCGCTCTATTCTGCGGTGGAGAATCATCTGATCC
CGTCCCGAAACGAGGCAGTT

Downstream 100 bases:

>100_bases
TCGCCGACTACTCTTCCTTCTTCGGTTGATCGAGCGACATCCGGTCGAGCCGCTCCTGCATGTCCCGCATCTGCCGACGC
AGATCGTCGATATTGTCGGC

Product: diguanylate cyclase/phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MSPVDPTAAQPIAPTESRGPDPAEILAALGQAAFVWELATDALVWTGPAAGLFQDIPAGTLATGAGYASLLEPSRAIRAD
ALLNASAAAGAHCVPYRIEYGVRAATSAPIIWIEESGRWHPGPDGRPARVEGIVRVNNERHARDQQLLKLSQNDPLTGEL
NRTNLVAALAEAIEEASRFRNSLCFMLIGIDHLSRINDSFGFDVADEVIYTLSQRIRARLRAGDELGRFSGNKFGVVLKA
CTIDDAHVAAERFLAAIRDEIVPTRSGPVSITASIGAVSSPRHARTAEEAINRAQEALAAAKHRRCGAFSMWRPNVERDA
QRRVNIRVTDEIVTALNERRIVLAYEPVVDVATRAPAFHECLVRMRQNDGQVLLAPDIVPVAERLGLIRLVDHRVLELVV
AELAAAPQMTLSLNISPDTTMDADWWAGIESLMLAHPGVAERLIVEITETVAIQDIEEVRGFVTRLKKFGAKIAIDDFGA
GYTSFRNLRNLGVDIVKIDGAFVQNIARSADDRAFVQTLIDLARRLGIKTVAEWVQDDESALMLRDWGCDYIQGRLIGLA
SQHRPWDGPTAAETAAVRERAAN

Sequences:

>Translated_583_residues
MSPVDPTAAQPIAPTESRGPDPAEILAALGQAAFVWELATDALVWTGPAAGLFQDIPAGTLATGAGYASLLEPSRAIRAD
ALLNASAAAGAHCVPYRIEYGVRAATSAPIIWIEESGRWHPGPDGRPARVEGIVRVNNERHARDQQLLKLSQNDPLTGEL
NRTNLVAALAEAIEEASRFRNSLCFMLIGIDHLSRINDSFGFDVADEVIYTLSQRIRARLRAGDELGRFSGNKFGVVLKA
CTIDDAHVAAERFLAAIRDEIVPTRSGPVSITASIGAVSSPRHARTAEEAINRAQEALAAAKHRRCGAFSMWRPNVERDA
QRRVNIRVTDEIVTALNERRIVLAYEPVVDVATRAPAFHECLVRMRQNDGQVLLAPDIVPVAERLGLIRLVDHRVLELVV
AELAAAPQMTLSLNISPDTTMDADWWAGIESLMLAHPGVAERLIVEITETVAIQDIEEVRGFVTRLKKFGAKIAIDDFGA
GYTSFRNLRNLGVDIVKIDGAFVQNIARSADDRAFVQTLIDLARRLGIKTVAEWVQDDESALMLRDWGCDYIQGRLIGLA
SQHRPWDGPTAAETAAVRERAAN
>Mature_582_residues
SPVDPTAAQPIAPTESRGPDPAEILAALGQAAFVWELATDALVWTGPAAGLFQDIPAGTLATGAGYASLLEPSRAIRADA
LLNASAAAGAHCVPYRIEYGVRAATSAPIIWIEESGRWHPGPDGRPARVEGIVRVNNERHARDQQLLKLSQNDPLTGELN
RTNLVAALAEAIEEASRFRNSLCFMLIGIDHLSRINDSFGFDVADEVIYTLSQRIRARLRAGDELGRFSGNKFGVVLKAC
TIDDAHVAAERFLAAIRDEIVPTRSGPVSITASIGAVSSPRHARTAEEAINRAQEALAAAKHRRCGAFSMWRPNVERDAQ
RRVNIRVTDEIVTALNERRIVLAYEPVVDVATRAPAFHECLVRMRQNDGQVLLAPDIVPVAERLGLIRLVDHRVLELVVA
ELAAAPQMTLSLNISPDTTMDADWWAGIESLMLAHPGVAERLIVEITETVAIQDIEEVRGFVTRLKKFGAKIAIDDFGAG
YTSFRNLRNLGVDIVKIDGAFVQNIARSADDRAFVQTLIDLARRLGIKTVAEWVQDDESALMLRDWGCDYIQGRLIGLAS
QHRPWDGPTAAETAAVRERAAN

Specific function: Unknown

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 MHYT domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=428, Percent_Identity=28.0373831775701, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1787541, Length=429, Percent_Identity=24.7086247086247, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI87081921, Length=434, Percent_Identity=24.4239631336406, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1790496, Length=230, Percent_Identity=30, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI87082096, Length=245, Percent_Identity=31.0204081632653, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI226510982, Length=403, Percent_Identity=25.8064516129032, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788502, Length=242, Percent_Identity=29.7520661157025, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1787055, Length=427, Percent_Identity=22.4824355971897, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87081743, Length=235, Percent_Identity=31.9148936170213, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87081980, Length=238, Percent_Identity=29.8319327731092, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1786507, Length=249, Percent_Identity=26.1044176706827, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787262, Length=160, Percent_Identity=33.125, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788381, Length=423, Percent_Identity=22.4586288416076, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI87081845, Length=225, Percent_Identity=27.1111111111111, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1786584, Length=162, Percent_Identity=29.0123456790123, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787410, Length=223, Percent_Identity=26.9058295964126, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI1789650, Length=408, Percent_Identity=19.8529411764706, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI145693134, Length=164, Percent_Identity=28.6585365853659, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR005330 [H]

Pfam domain/function: PF00563 EAL; PF00990 GGDEF; PF03707 MHYT [H]

EC number: NA

Molecular weight: Translated: 63509; Mature: 63378

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVDPTAAQPIAPTESRGPDPAEILAALGQAAFVWELATDALVWTGPAAGLFQDIPAGT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCEEECCCCCHHHHHCCCCC
LATGAGYASLLEPSRAIRADALLNASAAAGAHCVPYRIEYGVRAATSAPIIWIEESGRWH
HHCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCEECCCCCEEEEECCCCCC
PGPDGRPARVEGIVRVNNERHARDQQLLKLSQNDPLTGELNRTNLVAALAEAIEEASRFR
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NSLCFMLIGIDHLSRINDSFGFDVADEVIYTLSQRIRARLRAGDELGRFSGNKFGVVLKA
HHHEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEEE
CTIDDAHVAAERFLAAIRDEIVPTRSGPVSITASIGAVSSPRHARTAEEAINRAQEALAA
EECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
AKHRRCGAFSMWRPNVERDAQRRVNIRVTDEIVTALNERRIVLAYEPVVDVATRAPAFHE
HHHCCCCCCCCCCCCCHHHHHHEEEEEEHHHHHHHHCCCEEEEEECCHHHHHHCCCHHHH
CLVRMRQNDGQVLLAPDIVPVAERLGLIRLVDHRVLELVVAELAAAPQMTLSLNISPDTT
HHHHHHCCCCCEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
MDADWWAGIESLMLAHPGVAERLIVEITETVAIQDIEEVRGFVTRLKKFGAKIAIDDFGA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
GYTSFRNLRNLGVDIVKIDGAFVQNIARSADDRAFVQTLIDLARRLGIKTVAEWVQDDES
CHHHHHHHHHCCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
ALMLRDWGCDYIQGRLIGLASQHRPWDGPTAAETAAVRERAAN
EEEEECCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SPVDPTAAQPIAPTESRGPDPAEILAALGQAAFVWELATDALVWTGPAAGLFQDIPAGT
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHCCEEECCCCCHHHHHCCCCC
LATGAGYASLLEPSRAIRADALLNASAAAGAHCVPYRIEYGVRAATSAPIIWIEESGRWH
HHCCCCHHHHHCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCEECCCCCEEEEECCCCCC
PGPDGRPARVEGIVRVNNERHARDQQLLKLSQNDPLTGELNRTNLVAALAEAIEEASRFR
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
NSLCFMLIGIDHLSRINDSFGFDVADEVIYTLSQRIRARLRAGDELGRFSGNKFGVVLKA
HHHEEEEECHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEEE
CTIDDAHVAAERFLAAIRDEIVPTRSGPVSITASIGAVSSPRHARTAEEAINRAQEALAA
EECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
AKHRRCGAFSMWRPNVERDAQRRVNIRVTDEIVTALNERRIVLAYEPVVDVATRAPAFHE
HHHCCCCCCCCCCCCCHHHHHHEEEEEEHHHHHHHHCCCEEEEEECCHHHHHHCCCHHHH
CLVRMRQNDGQVLLAPDIVPVAERLGLIRLVDHRVLELVVAELAAAPQMTLSLNISPDTT
HHHHHHCCCCCEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
MDADWWAGIESLMLAHPGVAERLIVEITETVAIQDIEEVRGFVTRLKKFGAKIAIDDFGA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
GYTSFRNLRNLGVDIVKIDGAFVQNIARSADDRAFVQTLIDLARRLGIKTVAEWVQDDES
CHHHHHHHHHCCCEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCC
ALMLRDWGCDYIQGRLIGLASQHRPWDGPTAAETAAVRERAAN
EEEEECCCCHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]