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The map label for this gene is rpoH [H]

Identifier: 86747581

GI number: 86747581

Start: 502023

End: 502922

Strand: Direct

Name: rpoH [H]

Synonym: RPB_0455

Alternate gene names: 86747581

Gene position: 502023-502922 (Clockwise)

Preceding gene: 86747580

Following gene: 86747584

Centisome position: 9.42

GC content: 65.11

Gene sequence:

>900_bases
ATGGCCCGTGCAGCTACGCTACCCGTTCTCAACGGTGAATCCGGACTCGCTCGGTATCTCGCGGAAATCCGCAAGTTCCC
GATGCTCGAGCCGCAACAGGAATACATGTTCGCCAAGCGCTGGCGCGAGCACGATGATCGCGACGCCGCGCATCACCTCG
TCACCAGCCATCTGCGGCTCGTCGCCAAGATCGCCATGGGCTATCGCGGCTACGGCCTGCCGATCTCCGAGGTCGTCTCG
GAAGGCAATGTCGGCCTGATGCAGGCGGTGAAGCGGTTCGAGCCGGACAAAGGCTTCCGCCTCGCCACCTACGCGATGTG
GTGGATCAAGGCGTCGATTCAAGAATACATCCTGCGTTCGTGGTCGCTCGTGAAGATGGGCACCACCGCGAACCAGAAGA
AGCTGTTCTTCAATCTGCGCAAGGCGAAGAGCAAGATCTCGGCGCTGGACGAGGGTGATATGCACCCCGACCAGGTCAAG
CTGATCGCCAAGCGGCTCGGCGTCACCGAGCAGGACGTGATCGACATGAATCGCCGCCTCGGTGGCGACGCGTCGCTCAA
CGCCCCGATCCGCGACGACGGCGAGCCCGGCGAATGGCAGGACTGGCTGGTCGACCAGTCGCCGAATCAGGAAGCCGTGA
TGGCCGAGCACGAGGAGCTCGATCATCGCCGCGCCGCGCTGAACGGTGCGATCGGCGTGCTCAACCCGCGCGAACGGCGG
ATCTTCGAGGCGCGCCGCCTCGCCGACGAGCCGATGACGCTGGAAGACCTCGCCGCCGAGTTCGGCGTCTCGCGCGAGCG
CGTCCGCCAGATCGAGGTGCGTGCCTTCGAGAAGGTGCAGAGCGCCGTCAAGGGCACCATCGCGCGTCAGGAACAGGCGG
CGCTCGAAGCCGCCCACTGA

Upstream 100 bases:

>100_bases
ATGAGCCAACGCGGAACATCGCAGCCCGGTCGGCTTTAGCAGAGCGACCTGTCTGCCAGGCCGCCCGATGGCGGGGGCTT
GAAACCACTGGAGGGCGCTG

Downstream 100 bases:

>100_bases
CGCCGCCGCGCCAAGCGCCCTGCAACGACAAACCCCGCCGCATCCCGGCGGGGTTTTTGTTTGTCTGATGGAGCTTCGTC
GTCGCGGCGCGTTCAGCGCC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MARAATLPVLNGESGLARYLAEIRKFPMLEPQQEYMFAKRWREHDDRDAAHHLVTSHLRLVAKIAMGYRGYGLPISEVVS
EGNVGLMQAVKRFEPDKGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKKLFFNLRKAKSKISALDEGDMHPDQVK
LIAKRLGVTEQDVIDMNRRLGGDASLNAPIRDDGEPGEWQDWLVDQSPNQEAVMAEHEELDHRRAALNGAIGVLNPRERR
IFEARRLADEPMTLEDLAAEFGVSRERVRQIEVRAFEKVQSAVKGTIARQEQAALEAAH

Sequences:

>Translated_299_residues
MARAATLPVLNGESGLARYLAEIRKFPMLEPQQEYMFAKRWREHDDRDAAHHLVTSHLRLVAKIAMGYRGYGLPISEVVS
EGNVGLMQAVKRFEPDKGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKKLFFNLRKAKSKISALDEGDMHPDQVK
LIAKRLGVTEQDVIDMNRRLGGDASLNAPIRDDGEPGEWQDWLVDQSPNQEAVMAEHEELDHRRAALNGAIGVLNPRERR
IFEARRLADEPMTLEDLAAEFGVSRERVRQIEVRAFEKVQSAVKGTIARQEQAALEAAH
>Mature_298_residues
ARAATLPVLNGESGLARYLAEIRKFPMLEPQQEYMFAKRWREHDDRDAAHHLVTSHLRLVAKIAMGYRGYGLPISEVVSE
GNVGLMQAVKRFEPDKGFRLATYAMWWIKASIQEYILRSWSLVKMGTTANQKKLFFNLRKAKSKISALDEGDMHPDQVKL
IAKRLGVTEQDVIDMNRRLGGDASLNAPIRDDGEPGEWQDWLVDQSPNQEAVMAEHEELDHRRAALNGAIGVLNPRERRI
FEARRLADEPMTLEDLAAEFGVSRERVRQIEVRAFEKVQSAVKGTIARQEQAALEAAH

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=289, Percent_Identity=39.1003460207612, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789098, Length=287, Percent_Identity=31.7073170731707, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789448, Length=249, Percent_Identity=31.3253012048193, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33955; Mature: 33824

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARAATLPVLNGESGLARYLAEIRKFPMLEPQQEYMFAKRWREHDDRDAAHHLVTSHLRL
CCCCCCCCEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VAKIAMGYRGYGLPISEVVSEGNVGLMQAVKRFEPDKGFRLATYAMWWIKASIQEYILRS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTANQKKLFFNLRKAKSKISALDEGDMHPDQVKLIAKRLGVTEQDVIDMNRRL
CCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHC
GGDASLNAPIRDDGEPGEWQDWLVDQSPNQEAVMAEHEELDHRRAALNGAIGVLNPRERR
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
IFEARRLADEPMTLEDLAAEFGVSRERVRQIEVRAFEKVQSAVKGTIARQEQAALEAAH
HHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARAATLPVLNGESGLARYLAEIRKFPMLEPQQEYMFAKRWREHDDRDAAHHLVTSHLRL
CCCCCCCEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VAKIAMGYRGYGLPISEVVSEGNVGLMQAVKRFEPDKGFRLATYAMWWIKASIQEYILRS
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHC
WSLVKMGTTANQKKLFFNLRKAKSKISALDEGDMHPDQVKLIAKRLGVTEQDVIDMNRRL
CCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHC
GGDASLNAPIRDDGEPGEWQDWLVDQSPNQEAVMAEHEELDHRRAALNGAIGVLNPRERR
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
IFEARRLADEPMTLEDLAAEFGVSRERVRQIEVRAFEKVQSAVKGTIARQEQAALEAAH
HHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]