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The map label for this gene is mdeA [H]

Identifier: 86747576

GI number: 86747576

Start: 495742

End: 496941

Strand: Direct

Name: mdeA [H]

Synonym: RPB_0450

Alternate gene names: 86747576

Gene position: 495742-496941 (Clockwise)

Preceding gene: 86747574

Following gene: 86747577

Centisome position: 9.3

GC content: 68.5

Gene sequence:

>1200_bases
ATGAGCGATCTGTCCAACCGCCATCTCCCGAACAGTCTCGGCTTCGCAACCCGCGCCATCCACCATGGCTACGACCCGCA
GGCGCATCTCGGCAGCCTCAACCCGCCGGTGTTCCTGACCTCGACCTATGCGTTCGACAGCGCCGCCGAGGGCGCGGCGC
GGTTCGCCGGCGAGGCCGAGGGCTTCATCTATGGCCGGGTCGGCAACCCCACCGTCGCGGTGCTGGAGCGGCGGCTGGCC
GAGCTGGAAGGCGGCGAAGCGGCGCTCGCCACCTCCTCGGGCGTCGGCGCGCTGACCGCTTTGGTCTGGACGCTGCTCGC
CAGCGGCGACGAGATCGTCGCCGACCAGATGCTGTATGGCTGCACCTTCGGCCTGTTCGAGCATCATCTGCCGCGCTTCG
GCATCCGGGTGCGGTTCGCCGACCTCACCGATCCGGCCGGCCTGGAGGCGGCGCTGAGCCCGGCGACCAGGATGGTGTTC
ACCGAGACGCCGTCGAATCCGAACATGCGGGTGGTCGATATCGCCGCCATCGCGGCGATCACCCGCCGCGCCGGCGCGCT
GTTCGTCGTCGACAACACCTATTGCACGCCGTATCTGCAGCGCCCGCTCGAACTCGGCGCCGATATCGTCACCCATTCGG
CGACCAAATATCTCGGCGGCCATGGCGATCTGCTGGCCGGCGCCGTGGTGGCGTCGCAGGAGATCATCGACCGGCTGCGC
TTCACCGGCATCAAGGAACTCAACGGCGCCTGCATCTCGGCGTTCGACGCCTTTCTGATCCTGCGCGGGCTGAAGACGCT
GACGCTGCGGATGGACCGCCACAGCGCCACCGCCGCAAAACTCGCGCGCGACCTCGAGGCCCATCCGGCGGTGGCGGCTG
TGTACTATCCGGGCCTCGACAGCCATCCGCAGCACGCACTCGCGCAAAGGCAGATGCGCCAGATGGGCGGCATGATCGCG
CTGGAACTGAAAGGCGGACTACCAGCCGGCCGCGCGCTGATGGACAACGTGAAGATCGCGACCCGCGCGGTCAGCCTCGG
CGACGCCGAAACCCTGATCCAGCACCCCGCCAGCATGACGCACGCCACCTACGACCCCGACGAACGCGCCCGGCACGGGT
TCACCGACGGGCTGATCCGGATCTCGGTGGGGCTGGAGGACTACGAGGATTTGCGGGGGGATCTGCTGGGGGCGTTGTGA

Upstream 100 bases:

>100_bases
CAGCAAATTGCCAGATATCGCCAATTTGTAACTCCATAGTAGCATCTTTGGCAACATTGGCAGGCGGACTCACGCCATAC
TGGCGGCAAAGGAGCCTGCC

Downstream 100 bases:

>100_bases
GCGCCGATACACGCAGGCGTTGAAGTATCCGGCTCGGAACCTGCGTCCGGCTTCACCAAAACTATACCACGATGGCGAAC
GCTCCGACCCGGTTGACACT

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSDLSNRHLPNSLGFATRAIHHGYDPQAHLGSLNPPVFLTSTYAFDSAAEGAARFAGEAEGFIYGRVGNPTVAVLERRLA
ELEGGEAALATSSGVGALTALVWTLLASGDEIVADQMLYGCTFGLFEHHLPRFGIRVRFADLTDPAGLEAALSPATRMVF
TETPSNPNMRVVDIAAIAAITRRAGALFVVDNTYCTPYLQRPLELGADIVTHSATKYLGGHGDLLAGAVVASQEIIDRLR
FTGIKELNGACISAFDAFLILRGLKTLTLRMDRHSATAAKLARDLEAHPAVAAVYYPGLDSHPQHALAQRQMRQMGGMIA
LELKGGLPAGRALMDNVKIATRAVSLGDAETLIQHPASMTHATYDPDERARHGFTDGLIRISVGLEDYEDLRGDLLGAL

Sequences:

>Translated_399_residues
MSDLSNRHLPNSLGFATRAIHHGYDPQAHLGSLNPPVFLTSTYAFDSAAEGAARFAGEAEGFIYGRVGNPTVAVLERRLA
ELEGGEAALATSSGVGALTALVWTLLASGDEIVADQMLYGCTFGLFEHHLPRFGIRVRFADLTDPAGLEAALSPATRMVF
TETPSNPNMRVVDIAAIAAITRRAGALFVVDNTYCTPYLQRPLELGADIVTHSATKYLGGHGDLLAGAVVASQEIIDRLR
FTGIKELNGACISAFDAFLILRGLKTLTLRMDRHSATAAKLARDLEAHPAVAAVYYPGLDSHPQHALAQRQMRQMGGMIA
LELKGGLPAGRALMDNVKIATRAVSLGDAETLIQHPASMTHATYDPDERARHGFTDGLIRISVGLEDYEDLRGDLLGAL
>Mature_398_residues
SDLSNRHLPNSLGFATRAIHHGYDPQAHLGSLNPPVFLTSTYAFDSAAEGAARFAGEAEGFIYGRVGNPTVAVLERRLAE
LEGGEAALATSSGVGALTALVWTLLASGDEIVADQMLYGCTFGLFEHHLPRFGIRVRFADLTDPAGLEAALSPATRMVFT
ETPSNPNMRVVDIAAIAAITRRAGALFVVDNTYCTPYLQRPLELGADIVTHSATKYLGGHGDLLAGAVVASQEIIDRLRF
TGIKELNGACISAFDAFLILRGLKTLTLRMDRHSATAAKLARDLEAHPAVAAVYYPGLDSHPQHALAQRQMRQMGGMIAL
ELKGGLPAGRALMDNVKIATRAVSLGDAETLIQHPASMTHATYDPDERARHGFTDGLIRISVGLEDYEDLRGDLLGAL

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=379, Percent_Identity=42.2163588390501, Blast_Score=281, Evalue=5e-76,
Organism=Homo sapiens, GI299473759, Length=379, Percent_Identity=39.0501319261214, Blast_Score=251, Evalue=6e-67,
Organism=Homo sapiens, GI25453487, Length=378, Percent_Identity=37.037037037037, Blast_Score=217, Evalue=2e-56,
Organism=Escherichia coli, GI1790375, Length=381, Percent_Identity=41.2073490813648, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1789383, Length=385, Percent_Identity=30.3896103896104, Blast_Score=161, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17560056, Length=377, Percent_Identity=43.7665782493369, Blast_Score=290, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI17538055, Length=377, Percent_Identity=43.7665782493369, Blast_Score=287, Evalue=7e-78,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=39.3530997304582, Blast_Score=255, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6319307, Length=401, Percent_Identity=38.1546134663342, Blast_Score=232, Evalue=7e-62,
Organism=Saccharomyces cerevisiae, GI6323333, Length=426, Percent_Identity=35.2112676056338, Blast_Score=218, Evalue=1e-57,
Organism=Saccharomyces cerevisiae, GI6321254, Length=409, Percent_Identity=31.2958435207824, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=339, Percent_Identity=29.7935103244838, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6321904, Length=399, Percent_Identity=27.5689223057644, Blast_Score=119, Evalue=7e-28,
Organism=Drosophila melanogaster, GI19922554, Length=400, Percent_Identity=41, Blast_Score=283, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 42651; Mature: 42520

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLSNRHLPNSLGFATRAIHHGYDPQAHLGSLNPPVFLTSTYAFDSAAEGAARFAGEAE
CCCCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCCC
GFIYGRVGNPTVAVLERRLAELEGGEAALATSSGVGALTALVWTLLASGDEIVADQMLYG
CEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
CTFGLFEHHLPRFGIRVRFADLTDPAGLEAALSPATRMVFTETPSNPNMRVVDIAAIAAI
HHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHCCCEEEEEEECCCCCCEEEEHHHHHHHH
TRRAGALFVVDNTYCTPYLQRPLELGADIVTHSATKYLGGHGDLLAGAVVASQEIIDRLR
HHCCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
FTGIKELNGACISAFDAFLILRGLKTLTLRMDRHSATAAKLARDLEAHPAVAAVYYPGLD
HCCHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCC
SHPQHALAQRQMRQMGGMIALELKGGLPAGRALMDNVKIATRAVSLGDAETLIQHPASMT
CCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHCHHHHHHEECCCCHHHHHHCCCHHC
HATYDPDERARHGFTDGLIRISVGLEDYEDLRGDLLGAL
CCCCCCHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SDLSNRHLPNSLGFATRAIHHGYDPQAHLGSLNPPVFLTSTYAFDSAAEGAARFAGEAE
CCCCCCCCCCHHHHHHHHHHCCCCCCHHCCCCCCCEEEEEECCCCCHHHHHHHHCCCCC
GFIYGRVGNPTVAVLERRLAELEGGEAALATSSGVGALTALVWTLLASGDEIVADQMLYG
CEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
CTFGLFEHHLPRFGIRVRFADLTDPAGLEAALSPATRMVFTETPSNPNMRVVDIAAIAAI
HHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHCCCEEEEEEECCCCCCEEEEHHHHHHHH
TRRAGALFVVDNTYCTPYLQRPLELGADIVTHSATKYLGGHGDLLAGAVVASQEIIDRLR
HHCCCEEEEEECCCCCHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
FTGIKELNGACISAFDAFLILRGLKTLTLRMDRHSATAAKLARDLEAHPAVAAVYYPGLD
HCCHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCC
SHPQHALAQRQMRQMGGMIALELKGGLPAGRALMDNVKIATRAVSLGDAETLIQHPASMT
CCHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHCHHHHHHEECCCCHHHHHHCCCHHC
HATYDPDERARHGFTDGLIRISVGLEDYEDLRGDLLGAL
CCCCCCHHHHHCCCCCCEEEEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]