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The map label for this gene is pcm1

Identifier: 86747573

GI number: 86747573

Start: 493703

End: 494440

Strand: Reverse

Name: pcm1

Synonym: RPB_0447

Alternate gene names: 86747573

Gene position: 494440-493703 (Counterclockwise)

Preceding gene: 86747575

Following gene: 86747572

Centisome position: 9.27

GC content: 73.04

Gene sequence:

>738_bases
ATGGCGGCTTCTGGCTCCCGGCATCCACCGATCAGCGACGATGCGACCAGCTTCGCCGCGCAACGCGAGCGGATGGTCGC
CCAGCAGATCAGCGCGCGCGGCGTCCGCGACCCGCTGGTGCTGGCGGCGATGCGCCAGGTGCCGCGCGAGGCGTTCCTGC
CGGAGCGGATGCGCGATCTCGCTTACGACGATTCGCCGCTGCCGATCGGCCACGGGCAGACCATCTCGCAGCCCTACATC
GTCGCCGCCATGATCGAGGCGCTGCAATTGAACGGCGGCGAGCGCGTGCTCGAAATCGGCGCCGGCTCCGGCTACGCCGC
CGCGGTGCTGGCGCAGATCGCCGGCGAGGTCACCACGATCGAACGCATCGGCGCGCTCGCCGACAAGGCCGCGGCGGCGC
TGGCGGCGCTCGGCATCGGCAATGTCCAGGTCCGGCAGGGCGACGGATCGCGCGGCTGGCCGCCGGGCGCGCCCTACGAC
GCGATCGTGGTGGCGGCCGGCGGGCCGCATCTGCCGCAATCGCTCAAGACGCAGCTCGCGATCGGCGGCCGGCTGGTGAT
GCCGGTCGGCGCCGATCAGTCCGCCCAGCGGCTCGTCCGGCTGACCCGCACAAGCGTCGACGATGTCCGCTGCGAACAGC
TCGCCGACGTACGTTTCGTGCCGCTGATCGGCGACGAGGGCTGGGCCTCGGTCGCGCCGGAGCCGCGGGCCGACCGCCCC
GCCACGGTGCGCAAATGA

Upstream 100 bases:

>100_bases
GATGCGGATCAATTTGGCCCGGCTCGCCGCTTCTTAGATTTGCCCGGCTCGGGTCGTGGCAGGCGACCAGCGCCAGGGTC
GACGATCAGGGGAGGTTGCG

Downstream 100 bases:

>100_bases
GGCATGCATCAGCGCGACATTGCGGCCCGACCCTTTCGGTCCGCCGCAGTGCTATACAGCCGCATCGGCGCACGGCCGCA
CGGCGGATCGATCGCCGGCG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 1; Protein L-isoaspartyl methyltransferase 1; Protein-beta-aspartate methyltransferase 1; PIMT 1

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MAASGSRHPPISDDATSFAAQRERMVAQQISARGVRDPLVLAAMRQVPREAFLPERMRDLAYDDSPLPIGHGQTISQPYI
VAAMIEALQLNGGERVLEIGAGSGYAAAVLAQIAGEVTTIERIGALADKAAAALAALGIGNVQVRQGDGSRGWPPGAPYD
AIVVAAGGPHLPQSLKTQLAIGGRLVMPVGADQSAQRLVRLTRTSVDDVRCEQLADVRFVPLIGDEGWASVAPEPRADRP
ATVRK

Sequences:

>Translated_245_residues
MAASGSRHPPISDDATSFAAQRERMVAQQISARGVRDPLVLAAMRQVPREAFLPERMRDLAYDDSPLPIGHGQTISQPYI
VAAMIEALQLNGGERVLEIGAGSGYAAAVLAQIAGEVTTIERIGALADKAAAALAALGIGNVQVRQGDGSRGWPPGAPYD
AIVVAAGGPHLPQSLKTQLAIGGRLVMPVGADQSAQRLVRLTRTSVDDVRCEQLADVRFVPLIGDEGWASVAPEPRADRP
ATVRK
>Mature_244_residues
AASGSRHPPISDDATSFAAQRERMVAQQISARGVRDPLVLAAMRQVPREAFLPERMRDLAYDDSPLPIGHGQTISQPYIV
AAMIEALQLNGGERVLEIGAGSGYAAAVLAQIAGEVTTIERIGALADKAAAALAALGIGNVQVRQGDGSRGWPPGAPYDA
IVVAAGGPHLPQSLKTQLAIGGRLVMPVGADQSAQRLVRLTRTSVDDVRCEQLADVRFVPLIGDEGWASVAPEPRADRPA
TVRK

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=175, Percent_Identity=37.7142857142857, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1789100, Length=209, Percent_Identity=44.9760765550239, Blast_Score=167, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI71983477, Length=197, Percent_Identity=32.48730964467, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI193207222, Length=197, Percent_Identity=31.4720812182741, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI17981723, Length=221, Percent_Identity=33.4841628959276, Blast_Score=110, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT1_RHOP2 (Q2J302)

Other databases:

- EMBL:   CP000250
- RefSeq:   YP_484069.1
- ProteinModelPortal:   Q2J302
- STRING:   Q2J302
- GeneID:   3910003
- GenomeReviews:   CP000250_GR
- KEGG:   rpb:RPB_0447
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   AIERIPR
- ProtClustDB:   CLSK538723
- BioCyc:   RPAL316058:RPB_0447-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 25735; Mature: 25603

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 76-76

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASGSRHPPISDDATSFAAQRERMVAQQISARGVRDPLVLAAMRQVPREAFLPERMRDL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCHHHHHHH
AYDDSPLPIGHGQTISQPYIVAAMIEALQLNGGERVLEIGAGSGYAAAVLAQIAGEVTTI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
ERIGALADKAAAALAALGIGNVQVRQGDGSRGWPPGAPYDAIVVAAGGPHLPQSLKTQLA
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHH
IGGRLVMPVGADQSAQRLVRLTRTSVDDVRCEQLADVRFVPLIGDEGWASVAPEPRADRP
CCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCC
ATVRK
CCCCC
>Mature Secondary Structure 
AASGSRHPPISDDATSFAAQRERMVAQQISARGVRDPLVLAAMRQVPREAFLPERMRDL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCHHHHHHH
AYDDSPLPIGHGQTISQPYIVAAMIEALQLNGGERVLEIGAGSGYAAAVLAQIAGEVTTI
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHH
ERIGALADKAAAALAALGIGNVQVRQGDGSRGWPPGAPYDAIVVAAGGPHLPQSLKTQLA
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHH
IGGRLVMPVGADQSAQRLVRLTRTSVDDVRCEQLADVRFVPLIGDEGWASVAPEPRADRP
CCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCCCCC
ATVRK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA