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The map label for this gene is nth [H]

Identifier: 86747565

GI number: 86747565

Start: 482003

End: 482767

Strand: Direct

Name: nth [H]

Synonym: RPB_0439

Alternate gene names: 86747565

Gene position: 482003-482767 (Clockwise)

Preceding gene: 86747561

Following gene: 86747571

Centisome position: 9.04

GC content: 65.88

Gene sequence:

>765_bases
ATGTCGAAAATCACCCGCCGCGTGGCCGCTCCCGCCGCCCCGAGAACCGCCAAGACTACCCCGAAAAGCCTTGCGGCAAA
CCCGGGGCAAACCGGAGGCAAACCTGCGAAGCCGCCAAAACGCCCCCGCCGCTGGACTCCAGACGAGGTTCGCGAGGCTT
TCACCCGCTTCGCCCGCGCCAACCCGGAGCCGAAGGGTGAGCTCGAACATCTCAACCCGTTCACCCTGCTGGTCGCGGTC
GTGTTGTCGGCGCAGGCGACCGATGCCGGCGTCAACAAGGCGACTCGGAGTTTGTTCGCGATCGCCGATACGCCGGCCAA
GATGCTGGCGCTCGGCGAGGAGCGGGTGCGGGAGCATATCAAGACCATCGGCCTGTTTCGCACCAAGGCGAAAAACGTGA
TTGCGCTGTCGCAAAAACTGCTCTCCGACTTTGGTGGGCAAGTCCCAAGCACGCGCGCCGAGCTGGAGACGTTGCCGGGG
GCGGGCCGCAAGACCGCCAATGTGGTGCTCAACATGGCGTTCGGTCAGCCCACCATGGCGGTCGATACCCATGTGTTTCG
CGTCGGCAACCGCACCGGGCTCGCGCCCGGCGACACGCCGCTCGCGGTCGAACTCGGGCTCGAGAAGGCGATCCCGCCGG
AGTTCATGCAGCACGCGCACCATTGGCTGATCCTGCACGGCCGCTACACCTGCCTGGCCCGCAAGCCGCGCTGCGAGGTC
TGCCTGATCGTCGATCTGTGCAGGTGGCCGGAGAAAAGTGTGTAG

Upstream 100 bases:

>100_bases
GGCGCGATGCGGCGTCAGCAGCGCCGAAAACAGTTTCGGCTCGGCCCGATCCGGATCAAGATCGCTGTCTGTCGTCATTG
CGGAATATTATACCTCGCCC

Downstream 100 bases:

>100_bases
TTCCGTCTCCCGACGAGTAGCAACGTCATCCTGAGGTGCGCGCTCTTTCTAGCGCGCCTCGAAGGATGGGCCGCTTGTGT
CTGCGTCCGCATCCTTCGAG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSKITRRVAAPAAPRTAKTTPKSLAANPGQTGGKPAKPPKRPRRWTPDEVREAFTRFARANPEPKGELEHLNPFTLLVAV
VLSAQATDAGVNKATRSLFAIADTPAKMLALGEERVREHIKTIGLFRTKAKNVIALSQKLLSDFGGQVPSTRAELETLPG
AGRKTANVVLNMAFGQPTMAVDTHVFRVGNRTGLAPGDTPLAVELGLEKAIPPEFMQHAHHWLILHGRYTCLARKPRCEV
CLIVDLCRWPEKSV

Sequences:

>Translated_254_residues
MSKITRRVAAPAAPRTAKTTPKSLAANPGQTGGKPAKPPKRPRRWTPDEVREAFTRFARANPEPKGELEHLNPFTLLVAV
VLSAQATDAGVNKATRSLFAIADTPAKMLALGEERVREHIKTIGLFRTKAKNVIALSQKLLSDFGGQVPSTRAELETLPG
AGRKTANVVLNMAFGQPTMAVDTHVFRVGNRTGLAPGDTPLAVELGLEKAIPPEFMQHAHHWLILHGRYTCLARKPRCEV
CLIVDLCRWPEKSV
>Mature_253_residues
SKITRRVAAPAAPRTAKTTPKSLAANPGQTGGKPAKPPKRPRRWTPDEVREAFTRFARANPEPKGELEHLNPFTLLVAVV
LSAQATDAGVNKATRSLFAIADTPAKMLALGEERVREHIKTIGLFRTKAKNVIALSQKLLSDFGGQVPSTRAELETLPGA
GRKTANVVLNMAFGQPTMAVDTHVFRVGNRTGLAPGDTPLAVELGLEKAIPPEFMQHAHHWLILHGRYTCLARKPRCEVC
LIVDLCRWPEKSV

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=232, Percent_Identity=28.8793103448276, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1787920, Length=201, Percent_Identity=59.7014925373134, Blast_Score=246, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=32.2033898305085, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=29.9435028248588, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 27696; Mature: 27564

Theoretical pI: Translated: 10.92; Mature: 10.92

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKITRRVAAPAAPRTAKTTPKSLAANPGQTGGKPAKPPKRPRRWTPDEVREAFTRFARA
CCHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
NPEPKGELEHLNPFTLLVAVVLSAQATDAGVNKATRSLFAIADTPAKMLALGEERVREHI
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KTIGLFRTKAKNVIALSQKLLSDFGGQVPSTRAELETLPGAGRKTANVVLNMAFGQPTMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCCCHH
VDTHVFRVGNRTGLAPGDTPLAVELGLEKAIPPEFMQHAHHWLILHGRYTCLARKPRCEV
HHHEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCEEEEEECCEEEEECCCCCCE
CLIVDLCRWPEKSV
EEEEHHHCCCCCCC
>Mature Secondary Structure 
SKITRRVAAPAAPRTAKTTPKSLAANPGQTGGKPAKPPKRPRRWTPDEVREAFTRFARA
CHHHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
NPEPKGELEHLNPFTLLVAVVLSAQATDAGVNKATRSLFAIADTPAKMLALGEERVREHI
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KTIGLFRTKAKNVIALSQKLLSDFGGQVPSTRAELETLPGAGRKTANVVLNMAFGQPTMA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCEEEEEEECCCCCCHH
VDTHVFRVGNRTGLAPGDTPLAVELGLEKAIPPEFMQHAHHWLILHGRYTCLARKPRCEV
HHHEEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHCCCEEEEEECCEEEEECCCCCCE
CLIVDLCRWPEKSV
EEEEHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]