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The map label for this gene is gacS [H]

Identifier: 86747539

GI number: 86747539

Start: 456237

End: 458186

Strand: Reverse

Name: gacS [H]

Synonym: RPB_0413

Alternate gene names: 86747539

Gene position: 458186-456237 (Counterclockwise)

Preceding gene: 86747540

Following gene: 86747538

Centisome position: 8.59

GC content: 70.1

Gene sequence:

>1950_bases
ATGAAGCGGTGGTTGCGATTCAGATCGAGACGGCGCGCGTTCGCCGGACGGTTTCCGCGGCTTGTGTTCGCGCTGCGCTG
GAGCATGATCTTTCTGGCGGCGTTCGGTGGCGCCTATGGGTTCATCATCGGCAGCCGCGCCGAATCCTCCGGCTACAACC
CGCATACTTTCGCGATCGGTGCGAGCTTCCTGTTCGCGCTCGCCTGCCTCGGCCTCGCCGTCCAGAGCATACGACTGCGC
TGGTTGCGCCGGCGCCTACAGGCGTTGGAGCGGCACAACGAGACGCTGGCGGACCGCAACTGGGAGCTGAGGGATGCCGA
GGAACGCGCGCGCGTCCTCCACCAAGCTCGCGACGAAGCGGACGCGCCACGCCGCGACGAAGACGCAACGCGGCGGAAAT
CGCGGCTGCTGGCGATGGCGTCACACGAGATCCGCACGCCGCTGAACGGCATCATCGGCATGAGCGGCCTGCTGCTCGAC
ACCGCGCTGACGCCCGAGCAGGCGACCTACGCCCGCGCGGTGAAGACTTCGGGCGACGCGCTGGCGACGCTGATCGACGA
GCTGCTCGACTATTCCCGGATCGAGGCCGGCAGGTTCGAACTCGACAGCCGGCCGTTCGCGCTGACCGCCCTGATCGAGG
ACATCGTCGAGCTGCTGGCGCCGCGGGCGCAGGCGGGCGCGCTGGAGATCGCGGCGGATATCGATGACCGGGTACCGTCG
CGGGTGATCGGCGACGCGGCGCGCTTGCGCCAGGTGCTGCTCAATCTCGCCGGCAACGCCATCAAGTTCACGTCCGTCGG
CGGCGTCGTGCTGATCGTCCAGCCGGGCGAGCGCGACGGCGAGATCAGCTTCGCGGTGCGCGACACCGGCATCGGCATCG
CCCGTGAGGCGCAGACGCGGATCTTCGGCGAATTCGAACAGGCCGACGACGGCATCGCCCGCAGCTTCGGTGGCACCGGG
CTGGGGCTGAGTATCAGCCAGCGGATCGTCGAGCGGATGGGCGGTCGGATCGCACTCGACAGCCAGCCGGGACGAGGCTC
CACCTTCACGGCCTCGATCCCGCTCACAGCGGCGGACGATTCCGGCGCCTCCCCCATTGTCCAGCCGCCCGACCTCACCG
GTCAGTCGATCATGATCGCGGCGCCGCAGACCATCGAGGCGACGCTGGTCGCCCAGCGGCTGCAGCGCTGGGGCGCGCAC
GTGCTGGCGTTGTCCGAACTCTCCGCCGCGCGCACGGCGCTGCCCGAACGCGCCTGGTCGGCCATCCTGATCGATGCCGG
CTTCGGCGCCGATGCGGCCGAGGCGCTGGCGCGGAGTGCCCGACCGCATGCGGCGCAACGCGTCGTCATGCTGACGCCTG
CCGCTCGCTCCGAACTATTGCCGGATCTGCCACCTGCATTCACCGGCTATCTCGTCAAGCCGCTGCGGGCGGCCTCGCTG
TCGGCGCGGCTGGCAGGGTCGTTGATCGACACCGTCGCGCCGGGGATCGCGGACGGCGATCCGCCGGAGACCGCGGCAAC
GGATGCGACGGCGCCAGGCGGCCGGCGGCTCTCCGTGCTGGTCGCAGAAGACAACGAGATCAACGCGCTGCTGATCCGCT
CGCTGCTGGCTCGGCTCGGTCATCGCGTGGCTGTCGCCGGCGATGGCGAGCAGGCGCTGCAAAGCTGGCGCGCGGCCGCG
GCCGACGGCGCGCCCTTCGATCTGGTGCTGATGGACGTGCAGATGCCGACCAGCGACGGCATCGCCGCCAGCCGGCAGAT
CCGCGCGCAGGAGGCCGTCGGCGACGGACGCCGCACGCCGATTCTGGCGCTAACGGCGAATGCGCTCGCAGAAGATCGTG
AGGCCTGCTTCGCGGCGGGTATGGACGGCTTTCTGGTCAAGCCGCTCGATCGCGACAAGCTGATGACGGCGCTCGACCGG
GTCGCGGCGGCGAGGCCGATGGTCGCGTAG

Upstream 100 bases:

>100_bases
CCCTGTCGGCAGAGCGCATCGCAACCATCGCGCGCTTCAAGCTGTTTGCAATGTCGAGTGCCGATGCTGGCGACTCCGGC
ATGGTCGATCGGATTGGCGA

Downstream 100 bases:

>100_bases
TTGTGGCATGCCGTTACCGGGTTTCGACCCGGACTACAAACTTCGAGCCGCTGTCACGCGGCGGCAATCATCGTGTCACA
TCGCTGTTGAAACGTCGTGA

Product: periplasmic sensor hybrid histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MKRWLRFRSRRRAFAGRFPRLVFALRWSMIFLAAFGGAYGFIIGSRAESSGYNPHTFAIGASFLFALACLGLAVQSIRLR
WLRRRLQALERHNETLADRNWELRDAEERARVLHQARDEADAPRRDEDATRRKSRLLAMASHEIRTPLNGIIGMSGLLLD
TALTPEQATYARAVKTSGDALATLIDELLDYSRIEAGRFELDSRPFALTALIEDIVELLAPRAQAGALEIAADIDDRVPS
RVIGDAARLRQVLLNLAGNAIKFTSVGGVVLIVQPGERDGEISFAVRDTGIGIAREAQTRIFGEFEQADDGIARSFGGTG
LGLSISQRIVERMGGRIALDSQPGRGSTFTASIPLTAADDSGASPIVQPPDLTGQSIMIAAPQTIEATLVAQRLQRWGAH
VLALSELSAARTALPERAWSAILIDAGFGADAAEALARSARPHAAQRVVMLTPAARSELLPDLPPAFTGYLVKPLRAASL
SARLAGSLIDTVAPGIADGDPPETAATDATAPGGRRLSVLVAEDNEINALLIRSLLARLGHRVAVAGDGEQALQSWRAAA
ADGAPFDLVLMDVQMPTSDGIAASRQIRAQEAVGDGRRTPILALTANALAEDREACFAAGMDGFLVKPLDRDKLMTALDR
VAAARPMVA

Sequences:

>Translated_649_residues
MKRWLRFRSRRRAFAGRFPRLVFALRWSMIFLAAFGGAYGFIIGSRAESSGYNPHTFAIGASFLFALACLGLAVQSIRLR
WLRRRLQALERHNETLADRNWELRDAEERARVLHQARDEADAPRRDEDATRRKSRLLAMASHEIRTPLNGIIGMSGLLLD
TALTPEQATYARAVKTSGDALATLIDELLDYSRIEAGRFELDSRPFALTALIEDIVELLAPRAQAGALEIAADIDDRVPS
RVIGDAARLRQVLLNLAGNAIKFTSVGGVVLIVQPGERDGEISFAVRDTGIGIAREAQTRIFGEFEQADDGIARSFGGTG
LGLSISQRIVERMGGRIALDSQPGRGSTFTASIPLTAADDSGASPIVQPPDLTGQSIMIAAPQTIEATLVAQRLQRWGAH
VLALSELSAARTALPERAWSAILIDAGFGADAAEALARSARPHAAQRVVMLTPAARSELLPDLPPAFTGYLVKPLRAASL
SARLAGSLIDTVAPGIADGDPPETAATDATAPGGRRLSVLVAEDNEINALLIRSLLARLGHRVAVAGDGEQALQSWRAAA
ADGAPFDLVLMDVQMPTSDGIAASRQIRAQEAVGDGRRTPILALTANALAEDREACFAAGMDGFLVKPLDRDKLMTALDR
VAAARPMVA
>Mature_649_residues
MKRWLRFRSRRRAFAGRFPRLVFALRWSMIFLAAFGGAYGFIIGSRAESSGYNPHTFAIGASFLFALACLGLAVQSIRLR
WLRRRLQALERHNETLADRNWELRDAEERARVLHQARDEADAPRRDEDATRRKSRLLAMASHEIRTPLNGIIGMSGLLLD
TALTPEQATYARAVKTSGDALATLIDELLDYSRIEAGRFELDSRPFALTALIEDIVELLAPRAQAGALEIAADIDDRVPS
RVIGDAARLRQVLLNLAGNAIKFTSVGGVVLIVQPGERDGEISFAVRDTGIGIAREAQTRIFGEFEQADDGIARSFGGTG
LGLSISQRIVERMGGRIALDSQPGRGSTFTASIPLTAADDSGASPIVQPPDLTGQSIMIAAPQTIEATLVAQRLQRWGAH
VLALSELSAARTALPERAWSAILIDAGFGADAAEALARSARPHAAQRVVMLTPAARSELLPDLPPAFTGYLVKPLRAASL
SARLAGSLIDTVAPGIADGDPPETAATDATAPGGRRLSVLVAEDNEINALLIRSLLARLGHRVAVAGDGEQALQSWRAAA
ADGAPFDLVLMDVQMPTSDGIAASRQIRAQEAVGDGRRTPILALTANALAEDREACFAAGMDGFLVKPLDRDKLMTALDR
VAAARPMVA

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=547, Percent_Identity=33.6380255941499, Blast_Score=275, Evalue=8e-75,
Organism=Escherichia coli, GI145693157, Length=549, Percent_Identity=31.1475409836066, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI48994928, Length=390, Percent_Identity=31.7948717948718, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI87081816, Length=350, Percent_Identity=34.5714285714286, Blast_Score=160, Evalue=3e-40,
Organism=Escherichia coli, GI1788713, Length=264, Percent_Identity=33.7121212121212, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788549, Length=221, Percent_Identity=30.316742081448, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=31.2236286919831, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI1788393, Length=231, Percent_Identity=28.5714285714286, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87082128, Length=227, Percent_Identity=28.6343612334802, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786600, Length=228, Percent_Identity=28.5087719298246, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1786912, Length=236, Percent_Identity=29.2372881355932, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1787894, Length=235, Percent_Identity=31.063829787234, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786783, Length=233, Percent_Identity=25.7510729613734, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1790437, Length=128, Percent_Identity=33.59375, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790346, Length=230, Percent_Identity=27.3913043478261, Blast_Score=66, Evalue=9e-12,
Organism=Escherichia coli, GI1789808, Length=236, Percent_Identity=26.6949152542373, Blast_Score=62, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322044, Length=152, Percent_Identity=34.2105263157895, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322000, Length=168, Percent_Identity=30.3571428571429, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 69519; Mature: 69519

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRWLRFRSRRRAFAGRFPRLVFALRWSMIFLAAFGGAYGFIIGSRAESSGYNPHTFAIG
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHH
ASFLFALACLGLAVQSIRLRWLRRRLQALERHNETLADRNWELRDAEERARVLHQARDEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHC
DAPRRDEDATRRKSRLLAMASHEIRTPLNGIIGMSGLLLDTALTPEQATYARAVKTSGDA
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCCCCHHHHHHHHHCCCHH
LATLIDELLDYSRIEAGRFELDSRPFALTALIEDIVELLAPRAQAGALEIAADIDDRVPS
HHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHCCHH
RVIGDAARLRQVLLNLAGNAIKFTSVGGVVLIVQPGERDGEISFAVRDTGIGIAREAQTR
HHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHH
IFGEFEQADDGIARSFGGTGLGLSISQRIVERMGGRIALDSQPGRGSTFTASIPLTAADD
HHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEEEEECCC
SGASPIVQPPDLTGQSIMIAAPQTIEATLVAQRLQRWGAHVLALSELSAARTALPERAWS
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCE
AILIDAGFGADAAEALARSARPHAAQRVVMLTPAARSELLPDLPPAFTGYLVKPLRAASL
EEEEECCCCCHHHHHHHHCCCCHHHCEEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHH
SARLAGSLIDTVAPGIADGDPPETAATDATAPGGRRLSVLVAEDNEINALLIRSLLARLG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCC
HRVAVAGDGEQALQSWRAAAADGAPFDLVLMDVQMPTSDGIAASRQIRAQEAVGDGRRTP
CEEEEECCCHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
ILALTANALAEDREACFAAGMDGFLVKPLDRDKLMTALDRVAAARPMVA
EEEEEHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKRWLRFRSRRRAFAGRFPRLVFALRWSMIFLAAFGGAYGFIIGSRAESSGYNPHTFAIG
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHH
ASFLFALACLGLAVQSIRLRWLRRRLQALERHNETLADRNWELRDAEERARVLHQARDEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHC
DAPRRDEDATRRKSRLLAMASHEIRTPLNGIIGMSGLLLDTALTPEQATYARAVKTSGDA
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHCCCCCCHHHHHHHHHCCCHH
LATLIDELLDYSRIEAGRFELDSRPFALTALIEDIVELLAPRAQAGALEIAADIDDRVPS
HHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHCCHH
RVIGDAARLRQVLLNLAGNAIKFTSVGGVVLIVQPGERDGEISFAVRDTGIGIAREAQTR
HHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCEEEEEEECCCCCHHHHHHH
IFGEFEQADDGIARSFGGTGLGLSISQRIVERMGGRIALDSQPGRGSTFTASIPLTAADD
HHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCEEEEEEEEEECCC
SGASPIVQPPDLTGQSIMIAAPQTIEATLVAQRLQRWGAHVLALSELSAARTALPERAWS
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHCE
AILIDAGFGADAAEALARSARPHAAQRVVMLTPAARSELLPDLPPAFTGYLVKPLRAASL
EEEEECCCCCHHHHHHHHCCCCHHHCEEEEECCCHHHHCCCCCCCHHHHHHHHHHHHHHH
SARLAGSLIDTVAPGIADGDPPETAATDATAPGGRRLSVLVAEDNEINALLIRSLLARLG
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCC
HRVAVAGDGEQALQSWRAAAADGAPFDLVLMDVQMPTSDGIAASRQIRAQEAVGDGRRTP
CEEEEECCCHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
ILALTANALAEDREACFAAGMDGFLVKPLDRDKLMTALDRVAAARPMVA
EEEEEHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]