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The map label for this gene is lpd3 [H]

Identifier: 86747400

GI number: 86747400

Start: 308513

End: 309916

Strand: Reverse

Name: lpd3 [H]

Synonym: RPB_0274

Alternate gene names: 86747400

Gene position: 309916-308513 (Counterclockwise)

Preceding gene: 86747401

Following gene: 86747399

Centisome position: 5.81

GC content: 66.24

Gene sequence:

>1404_bases
ATGGCCAACTACGATCTCGTCATCATCGGCACCGGCCCCGGCGGCTATGTCTGTGCGATCCGCGCGGCGCAGCTCGGCCT
CAAGGTCGCGGTGGTCGAAAAGAACCCCACGCTCGGCGGCACCTGCCTCAATGTCGGCTGCATGCCGTCGAAGGCGCTGT
TGCACGCCTCGGAGCTGTTTGAGGAGGCCGGGCACTCCTTCGCCAAGATGGGGATCGGCGTGCCGGCGCCGACGCTCGAT
CTGCCGACGATGATGAATTTCAAGCAGCAGGGCATCGACGGCAACGTCAAGGGCGTCGAGTACCTGATGAAGAAGAACAA
GATCGACGTGCTGGTCGGCCGCGGCAAGGTGCTCGGCACCGGCAAGGTCGAGGTCACCGGCAATGACGGCAAGGCGCAAA
CCGTCGAGACCAAGTCGATCGTGATCGCCACCGGCTCGGACGTGGCGAAGCTCAAGGGCATCGAGATCGACGAGAAGCGC
ATCGTGTCGTCGACCGGCGCGCTGTCGCTCGACAAGGTGCCGGGCAAGCTGATCGTGGTCGGCGCCGGCGTGATCGGGCT
CGAACTCGGCTCGGTGTGGCGCCGGCTCGGCGCCCAGGTCACCGTGGTCGAATTTCTCGACCGCATCCTGCCCGGCATGG
ATGCCGAGATCGTCAAGCAGTTCCAGCGCATCCTCGAGAAGCAGGGTTTTGCCTTCAAGCTCGGCGCCAAGGTCACCGGG
GTCGACAGTTCGGGCAAGCAGCTCAAGGTCAGCGTCGAGGCCGCGGCCGGCGGCAATCCGGAGACGCTCGAAGCCGACGT
CGTGCTGGTCGCGATCGGCCGCGTGCCCTTCACCGAGGGGCTCGGGCTGCAGGAAGCCGGCGTCGCGCTCGACGAGCGCG
GCCGCGTGGCGATCGACGATCACTTCGCCACAAGCGTCCAGGGCGTCTATGCGATCGGCGACGTGGTGAAGGGCCCGATG
CTGGCGCACAAGGCCGAGGACGAGGGCGTCGCGGTCGCGGAAATCATCGCCGGCAAGGCCGGGCACGTGAACTACGACGT
CATTCCGGGCGTGGTCTACACCACGCCGGAAGTCTCATCCGTCGGCAAGACCGAGGAAGACCTCAAGCAGGCCGGCGTCG
CCTACACGGTCGGCAAATTCCCGTTCACCGCCAACGGCCGCTCCAAGGTCAATCAGACCACCGACGGTCTGGTGAAGATC
CTGGCCGACGCCAAGACCGACCGCGTGCTCGGCGTGCACATCGTCGGCCGCGAAGCCGGCGAGATGATCCACGAGGCCGC
AGTGCTGATGGAGTTCGGCGGCTCGGCCGAGGATCTGGCACGGACCTGCCACGCTCACCCCACCCGCTCCGAAGCGGTCA
AGGAAGCCGCGCTTGCGGTCGGAAAACGCGCCATCCATATGTAA

Upstream 100 bases:

>100_bases
GGTCTGGCTGATGTCGGACGAAGCCTCCTACGTCACCAGCGCCATCATCGATGTCTCGGGCGGACGATAGTCCACCCTGC
GCCAACTACAGGACTTCCCT

Downstream 100 bases:

>100_bases
ACGAGTGCCGGCATCGTCCGGAAACCGGACGACTCCATGAAGCGCCGCCTGCTGCAACCGCTGTGGTTCATCCTCGCCGT
CCTCTTTCTGGTCGAGGCAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MANYDLVIIGTGPGGYVCAIRAAQLGLKVAVVEKNPTLGGTCLNVGCMPSKALLHASELFEEAGHSFAKMGIGVPAPTLD
LPTMMNFKQQGIDGNVKGVEYLMKKNKIDVLVGRGKVLGTGKVEVTGNDGKAQTVETKSIVIATGSDVAKLKGIEIDEKR
IVSSTGALSLDKVPGKLIVVGAGVIGLELGSVWRRLGAQVTVVEFLDRILPGMDAEIVKQFQRILEKQGFAFKLGAKVTG
VDSSGKQLKVSVEAAAGGNPETLEADVVLVAIGRVPFTEGLGLQEAGVALDERGRVAIDDHFATSVQGVYAIGDVVKGPM
LAHKAEDEGVAVAEIIAGKAGHVNYDVIPGVVYTTPEVSSVGKTEEDLKQAGVAYTVGKFPFTANGRSKVNQTTDGLVKI
LADAKTDRVLGVHIVGREAGEMIHEAAVLMEFGGSAEDLARTCHAHPTRSEAVKEAALAVGKRAIHM

Sequences:

>Translated_467_residues
MANYDLVIIGTGPGGYVCAIRAAQLGLKVAVVEKNPTLGGTCLNVGCMPSKALLHASELFEEAGHSFAKMGIGVPAPTLD
LPTMMNFKQQGIDGNVKGVEYLMKKNKIDVLVGRGKVLGTGKVEVTGNDGKAQTVETKSIVIATGSDVAKLKGIEIDEKR
IVSSTGALSLDKVPGKLIVVGAGVIGLELGSVWRRLGAQVTVVEFLDRILPGMDAEIVKQFQRILEKQGFAFKLGAKVTG
VDSSGKQLKVSVEAAAGGNPETLEADVVLVAIGRVPFTEGLGLQEAGVALDERGRVAIDDHFATSVQGVYAIGDVVKGPM
LAHKAEDEGVAVAEIIAGKAGHVNYDVIPGVVYTTPEVSSVGKTEEDLKQAGVAYTVGKFPFTANGRSKVNQTTDGLVKI
LADAKTDRVLGVHIVGREAGEMIHEAAVLMEFGGSAEDLARTCHAHPTRSEAVKEAALAVGKRAIHM
>Mature_466_residues
ANYDLVIIGTGPGGYVCAIRAAQLGLKVAVVEKNPTLGGTCLNVGCMPSKALLHASELFEEAGHSFAKMGIGVPAPTLDL
PTMMNFKQQGIDGNVKGVEYLMKKNKIDVLVGRGKVLGTGKVEVTGNDGKAQTVETKSIVIATGSDVAKLKGIEIDEKRI
VSSTGALSLDKVPGKLIVVGAGVIGLELGSVWRRLGAQVTVVEFLDRILPGMDAEIVKQFQRILEKQGFAFKLGAKVTGV
DSSGKQLKVSVEAAAGGNPETLEADVVLVAIGRVPFTEGLGLQEAGVALDERGRVAIDDHFATSVQGVYAIGDVVKGPML
AHKAEDEGVAVAEIIAGKAGHVNYDVIPGVVYTTPEVSSVGKTEEDLKQAGVAYTVGKFPFTANGRSKVNQTTDGLVKIL
ADAKTDRVLGVHIVGREAGEMIHEAAVLMEFGGSAEDLARTCHAHPTRSEAVKEAALAVGKRAIHM

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=56.9868995633188, Blast_Score=514, Evalue=1e-146,
Organism=Homo sapiens, GI50301238, Length=469, Percent_Identity=29.2110874200426, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI22035672, Length=488, Percent_Identity=31.3524590163934, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI33519430, Length=466, Percent_Identity=28.9699570815451, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI33519428, Length=466, Percent_Identity=28.9699570815451, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI33519426, Length=466, Percent_Identity=28.9699570815451, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI148277065, Length=466, Percent_Identity=29.1845493562232, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI148277071, Length=466, Percent_Identity=29.1845493562232, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI291045266, Length=471, Percent_Identity=30.7855626326964, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI291045268, Length=465, Percent_Identity=29.6774193548387, Blast_Score=126, Evalue=4e-29,
Organism=Escherichia coli, GI1786307, Length=453, Percent_Identity=42.1633554083885, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI87082354, Length=474, Percent_Identity=31.0126582278481, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI87081717, Length=460, Percent_Identity=29.7826086956522, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1789915, Length=442, Percent_Identity=28.9592760180996, Blast_Score=158, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI32565766, Length=456, Percent_Identity=56.578947368421, Blast_Score=516, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17557007, Length=494, Percent_Identity=30.9716599190283, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI71982272, Length=484, Percent_Identity=26.4462809917355, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=442, Percent_Identity=26.2443438914027, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=442, Percent_Identity=26.2443438914027, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17559934, Length=233, Percent_Identity=29.6137339055794, Blast_Score=82, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=50.7399577167019, Blast_Score=450, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=33.6864406779661, Blast_Score=250, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6325166, Length=469, Percent_Identity=27.5053304904051, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI21358499, Length=458, Percent_Identity=56.1135371179039, Blast_Score=509, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24640551, Length=491, Percent_Identity=30.142566191446, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24640549, Length=485, Percent_Identity=30.1030927835052, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24640553, Length=485, Percent_Identity=30.1030927835052, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17737741, Length=489, Percent_Identity=27.19836400818, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48760; Mature: 48629

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANYDLVIIGTGPGGYVCAIRAAQLGLKVAVVEKNPTLGGTCLNVGCMPSKALLHASELF
CCCCEEEEEECCCCCEEEEEEEHHCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHH
EEAGHSFAKMGIGVPAPTLDLPTMMNFKQQGIDGNVKGVEYLMKKNKIDVLVGRGKVLGT
HHHCCHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCEEEEC
GKVEVTGNDGKAQTVETKSIVIATGSDVAKLKGIEIDEKRIVSSTGALSLDKVPGKLIVV
CEEEEECCCCCCEEEEEEEEEEECCCCHHHCCCCCCCHHHHHHCCCCEEECCCCCEEEEE
GAGVIGLELGSVWRRLGAQVTVVEFLDRILPGMDAEIVKQFQRILEKQGFAFKLGAKVTG
ECCHHHHHHHHHHHHHCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCEEEC
VDSSGKQLKVSVEAAAGGNPETLEADVVLVAIGRVPFTEGLGLQEAGVALDERGRVAIDD
CCCCCCEEEEEEEECCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCEECCCCCEEEEC
HFATSVQGVYAIGDVVKGPMLAHKAEDEGVAVAEIIAGKAGHVNYDVIPGVVYTTPEVSS
CHHHHHCHHHHHHHHHCCCHHEECCCCCCCEEEHHHCCCCCCCCEEECCCEEEECCCCHH
VGKTEEDLKQAGVAYTVGKFPFTANGRSKVNQTTDGLVKILADAKTDRVLGVHIVGREAG
CCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCHHH
EMIHEAAVLMEFGGSAEDLARTCHAHPTRSEAVKEAALAVGKRAIHM
HHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANYDLVIIGTGPGGYVCAIRAAQLGLKVAVVEKNPTLGGTCLNVGCMPSKALLHASELF
CCCEEEEEECCCCCEEEEEEEHHCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHH
EEAGHSFAKMGIGVPAPTLDLPTMMNFKQQGIDGNVKGVEYLMKKNKIDVLVGRGKVLGT
HHHCCHHHHHCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCEEEEC
GKVEVTGNDGKAQTVETKSIVIATGSDVAKLKGIEIDEKRIVSSTGALSLDKVPGKLIVV
CEEEEECCCCCCEEEEEEEEEEECCCCHHHCCCCCCCHHHHHHCCCCEEECCCCCEEEEE
GAGVIGLELGSVWRRLGAQVTVVEFLDRILPGMDAEIVKQFQRILEKQGFAFKLGAKVTG
ECCHHHHHHHHHHHHHCCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCEEEC
VDSSGKQLKVSVEAAAGGNPETLEADVVLVAIGRVPFTEGLGLQEAGVALDERGRVAIDD
CCCCCCEEEEEEEECCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCEECCCCCEEEEC
HFATSVQGVYAIGDVVKGPMLAHKAEDEGVAVAEIIAGKAGHVNYDVIPGVVYTTPEVSS
CHHHHHCHHHHHHHHHCCCHHEECCCCCCCEEEHHHCCCCCCCCEEECCCEEEECCCCHH
VGKTEEDLKQAGVAYTVGKFPFTANGRSKVNQTTDGLVKILADAKTDRVLGVHIVGREAG
CCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCHHH
EMIHEAAVLMEFGGSAEDLARTCHAHPTRSEAVKEAALAVGKRAIHM
HHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1722146; 2914869 [H]