Click here to switch to the map view.

The map label for this gene is gsiD [H]

Identifier: 86747356

GI number: 86747356

Start: 262969

End: 263847

Strand: Reverse

Name: gsiD [H]

Synonym: RPB_0230

Alternate gene names: 86747356

Gene position: 263847-262969 (Counterclockwise)

Preceding gene: 86747357

Following gene: 86747355

Centisome position: 4.95

GC content: 68.03

Gene sequence:

>879_bases
ATGACCGATGTGGCGATGACGGCAATGGCGCATCGGCCGCGACGGATGCGGCGTTTCGTGCGGTGGTTCGTCTCCGACAT
CCGCGGCGCGGTCAGTCTGACGTTCCTGCTGGTGCTGCTGGTGATCTCTCTGCTCGCGCCGTGGATCTCGCCCTACGATC
CGGTCGCGCAGGATGTCGACGCGGCGCTGGCGCCGATGTCGGCGGCGCATTGGCTCGGCGCCGACGATCTCGGCCGCGAC
ACGCTGAGCCGCCTGATCTACGGCGGGATGGCGTCGCTGTATGCGAGCTTCCTCGCGGTCGCGATCGCAGTGCTGATCGG
CGTGCCGATCGGCGTGCTCGCCGGCTATCTCGGCGGCTGGGTCGATACCATGATCAGCCGTGTCATCGACAGCTTCCTGT
CGTTCCCCGCCATCGTGCTGGCGATCGCCGTCACCGGCGCGCTCGGCATCGGACTCACCAACGGCATGATCTCGGTCGGC
ATCGTGTTCGCGCCGCAACTCGCGCGGCTGGTCCGCGCGCGCACGCTGGTGGTTCGCCACGAACTCTATGTCGACGCCGC
GCGCTGTTTCGGCGGCTCCACCGGCCGCATCCTGTGGCGTCACGTGCTGCCCAACGCGATCCAGCCGGTGATCGTGCAGG
TGACATTGCTGCTGGCGGCGGCGCTGCTCGCCGAAGCCAGCCTCAGCTTCCTTGGCCTCGGCATTCAGCCGCCCGATCCG
AGCTGGGGCGCGATGCTGGCGCGGGCCTATCAGAACATGGAGATCGCGCCGGAGCAGATGTATCCGCCGGGGCTCGCCAT
CCTGTTCACCGCGCTCGCCTACAACACGCTCGGCGAATCGCTGCGCGTCGCGCTCGACCCGACCATGAAGCGCAACTGA

Upstream 100 bases:

>100_bases
CGGTGGTGCAGGGCGTGGTGTTCACCATGGTGCTGATCGTGGTGTCGCTCAATCTCTTGACCGACATCCTCTACAGCGTT
CTCGATCCGAGGATCGCGGC

Downstream 100 bases:

>100_bases
CAAGACCAAGAACAACAAGGACAAGACCGATGAGCGCTGCCGCCAAGGAAGCCGACTATCTCGCAACGCTGCACGGGCTG
TGGGCCGAGTCCTGGCCCAA

Product: binding-protein dependent transport system inner membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTDVAMTAMAHRPRRMRRFVRWFVSDIRGAVSLTFLLVLLVISLLAPWISPYDPVAQDVDAALAPMSAAHWLGADDLGRD
TLSRLIYGGMASLYASFLAVAIAVLIGVPIGVLAGYLGGWVDTMISRVIDSFLSFPAIVLAIAVTGALGIGLTNGMISVG
IVFAPQLARLVRARTLVVRHELYVDAARCFGGSTGRILWRHVLPNAIQPVIVQVTLLLAAALLAEASLSFLGLGIQPPDP
SWGAMLARAYQNMEIAPEQMYPPGLAILFTALAYNTLGESLRVALDPTMKRN

Sequences:

>Translated_292_residues
MTDVAMTAMAHRPRRMRRFVRWFVSDIRGAVSLTFLLVLLVISLLAPWISPYDPVAQDVDAALAPMSAAHWLGADDLGRD
TLSRLIYGGMASLYASFLAVAIAVLIGVPIGVLAGYLGGWVDTMISRVIDSFLSFPAIVLAIAVTGALGIGLTNGMISVG
IVFAPQLARLVRARTLVVRHELYVDAARCFGGSTGRILWRHVLPNAIQPVIVQVTLLLAAALLAEASLSFLGLGIQPPDP
SWGAMLARAYQNMEIAPEQMYPPGLAILFTALAYNTLGESLRVALDPTMKRN
>Mature_291_residues
TDVAMTAMAHRPRRMRRFVRWFVSDIRGAVSLTFLLVLLVISLLAPWISPYDPVAQDVDAALAPMSAAHWLGADDLGRDT
LSRLIYGGMASLYASFLAVAIAVLIGVPIGVLAGYLGGWVDTMISRVIDSFLSFPAIVLAIAVTGALGIGLTNGMISVGI
VFAPQLARLVRARTLVVRHELYVDAARCFGGSTGRILWRHVLPNAIQPVIVQVTLLLAAALLAEASLSFLGLGIQPPDPS
WGAMLARAYQNMEIAPEQMYPPGLAILFTALAYNTLGESLRVALDPTMKRN

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787054, Length=284, Percent_Identity=42.9577464788732, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1787760, Length=284, Percent_Identity=40.4929577464789, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789964, Length=282, Percent_Identity=40.7801418439716, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI1789889, Length=244, Percent_Identity=37.2950819672131, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI1787549, Length=282, Percent_Identity=35.4609929078014, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1787498, Length=272, Percent_Identity=35.6617647058824, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1788505, Length=228, Percent_Identity=31.5789473684211, Blast_Score=94, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31365; Mature: 31233

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVAMTAMAHRPRRMRRFVRWFVSDIRGAVSLTFLLVLLVISLLAPWISPYDPVAQDVD
CCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AALAPMSAAHWLGADDLGRDTLSRLIYGGMASLYASFLAVAIAVLIGVPIGVLAGYLGGW
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
VDTMISRVIDSFLSFPAIVLAIAVTGALGIGLTNGMISVGIVFAPQLARLVRARTLVVRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELYVDAARCFGGSTGRILWRHVLPNAIQPVIVQVTLLLAAALLAEASLSFLGLGIQPPDP
HHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SWGAMLARAYQNMEIAPEQMYPPGLAILFTALAYNTLGESLRVALDPTMKRN
CHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDVAMTAMAHRPRRMRRFVRWFVSDIRGAVSLTFLLVLLVISLLAPWISPYDPVAQDVD
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
AALAPMSAAHWLGADDLGRDTLSRLIYGGMASLYASFLAVAIAVLIGVPIGVLAGYLGGW
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
VDTMISRVIDSFLSFPAIVLAIAVTGALGIGLTNGMISVGIVFAPQLARLVRARTLVVRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELYVDAARCFGGSTGRILWRHVLPNAIQPVIVQVTLLLAAALLAEASLSFLGLGIQPPDP
HHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
SWGAMLARAYQNMEIAPEQMYPPGLAILFTALAYNTLGESLRVALDPTMKRN
CHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]