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The map label for this gene is paaG [C]

Identifier: 86747238

GI number: 86747238

Start: 121412

End: 122239

Strand: Reverse

Name: paaG [C]

Synonym: RPB_0111

Alternate gene names: 86747238

Gene position: 122239-121412 (Counterclockwise)

Preceding gene: 86747239

Following gene: 86747237

Centisome position: 2.29

GC content: 67.51

Gene sequence:

>828_bases
ATGAGCACCCGGGACATCCTCTATCAGGACATCCTCTATGAGGTCGCCGACAGGATCGCGACGATCACGCTGAACCGGCC
GGACCGGATGAATGCGTGGACGCCGGTGATGGAGCGCGAGGTGCGCGAGGCGATGGAAACAGCCGCAGAAGACGCCGAGG
TCCGCGTCATCGTGCTGACCGGCGCCGGCCGCGGCTTCTGCGCCGGCGCCGACATGCAGGTGCTGCAGACCATCGATCCG
TCCGATGTCCGCCGCGCCTCGAGCCTGCCGCCGTTCGACATGAACCGCCGGGCCGACTGGCAAACCCGCTACGCGTTCTA
TCCGGCGATTCCCAAGCCGATCATCGGCATGCTGAACGGCGCCACCGCCGGAATCGGGCTGGTGCACGCGCTGTATTGCG
ACGTCCGCTTTGCCGCCGACAGCGCGGTGTTCACCACCGCGTTCGCGCGGCGCGGGCTGATCGCCGAGCACGGCATTTCC
TGGATGCTGCCGCGGATCGTCGGCCACGCCAATGCGCTCGACCTGTTGCTGTCGGCGCGGCGGGTCTCGGCCGAGGAGGC
GCTGCGGATTGGTCTCGTCAACCGGCTGGTTCCGTCCGAGCAGCTCCGCGAGCAGACCTACGCCTACGCCCGCGATCTCG
CCGACAATGTTTCGCCGGCGTCGATGCGGGTGATCAAGCGGCAGCTCTACGAGGTGCCGTTCCAGACGCTCGCCGAGGCG
ACCATCGAGGCCAATCGCGAGATGGTCGTCTCGCTCGGCAGCGACGATTTCAAGGAAGGCGTCGCCAGCTTCGTCGAGAA
GCGCCCGCCGCAGTTCCGCAGCGTGTGA

Upstream 100 bases:

>100_bases
TGCGCTTGCTACTCTGCGGCGCAGGCGGCGCGGAAGCGCACTGCCGCAGCGAGAATGCCCGGGAAGGGCGTAACAAAAAG
CAAAAAAGGGAGACACGCCG

Downstream 100 bases:

>100_bases
GGGCGATACGGCTACCACGCGTCATGCCCGGCCTTGTTGCCGGGCATCCACGCCTTGCGGCGGTCGCCTCGAACAAGACG
TGGATGGCCGGGACAAGCCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MSTRDILYQDILYEVADRIATITLNRPDRMNAWTPVMEREVREAMETAAEDAEVRVIVLTGAGRGFCAGADMQVLQTIDP
SDVRRASSLPPFDMNRRADWQTRYAFYPAIPKPIIGMLNGATAGIGLVHALYCDVRFAADSAVFTTAFARRGLIAEHGIS
WMLPRIVGHANALDLLLSARRVSAEEALRIGLVNRLVPSEQLREQTYAYARDLADNVSPASMRVIKRQLYEVPFQTLAEA
TIEANREMVVSLGSDDFKEGVASFVEKRPPQFRSV

Sequences:

>Translated_275_residues
MSTRDILYQDILYEVADRIATITLNRPDRMNAWTPVMEREVREAMETAAEDAEVRVIVLTGAGRGFCAGADMQVLQTIDP
SDVRRASSLPPFDMNRRADWQTRYAFYPAIPKPIIGMLNGATAGIGLVHALYCDVRFAADSAVFTTAFARRGLIAEHGIS
WMLPRIVGHANALDLLLSARRVSAEEALRIGLVNRLVPSEQLREQTYAYARDLADNVSPASMRVIKRQLYEVPFQTLAEA
TIEANREMVVSLGSDDFKEGVASFVEKRPPQFRSV
>Mature_274_residues
STRDILYQDILYEVADRIATITLNRPDRMNAWTPVMEREVREAMETAAEDAEVRVIVLTGAGRGFCAGADMQVLQTIDPS
DVRRASSLPPFDMNRRADWQTRYAFYPAIPKPIIGMLNGATAGIGLVHALYCDVRFAADSAVFTTAFARRGLIAEHGISW
MLPRIVGHANALDLLLSARRVSAEEALRIGLVNRLVPSEQLREQTYAYARDLADNVSPASMRVIKRQLYEVPFQTLAEAT
IEANREMVVSLGSDDFKEGVASFVEKRPPQFRSV

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=257, Percent_Identity=30.7392996108949, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI70995211, Length=229, Percent_Identity=28.82096069869, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI260274832, Length=261, Percent_Identity=24.5210727969349, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI45643119, Length=261, Percent_Identity=24.5210727969349, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI260275230, Length=261, Percent_Identity=24.5210727969349, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI31542718, Length=255, Percent_Identity=27.843137254902, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI4502327, Length=281, Percent_Identity=30.6049822064057, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI221136756, Length=209, Percent_Identity=28.2296650717703, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI221136753, Length=209, Percent_Identity=28.2296650717703, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI37594471, Length=206, Percent_Identity=27.6699029126214, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI37594469, Length=206, Percent_Identity=27.6699029126214, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI221307494, Length=191, Percent_Identity=29.8429319371728, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI68989263, Length=221, Percent_Identity=27.1493212669683, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI116235487, Length=191, Percent_Identity=29.8429319371728, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI4757968, Length=210, Percent_Identity=26.1904761904762, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI49355787, Length=210, Percent_Identity=26.1904761904762, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI25453481, Length=208, Percent_Identity=25.4807692307692, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI51538573, Length=208, Percent_Identity=25.4807692307692, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4757966, Length=208, Percent_Identity=25.4807692307692, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI51538581, Length=208, Percent_Identity=25.4807692307692, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI20127408, Length=228, Percent_Identity=28.5087719298246, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=34.9809885931559, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1788597, Length=269, Percent_Identity=33.0855018587361, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1787659, Length=264, Percent_Identity=31.0606060606061, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=29.7709923664122, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788682, Length=209, Percent_Identity=30.1435406698565, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082183, Length=278, Percent_Identity=26.6187050359712, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790281, Length=210, Percent_Identity=26.1904761904762, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=260, Percent_Identity=32.3076923076923, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=28.7937743190661, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=269, Percent_Identity=28.996282527881, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17536985, Length=221, Percent_Identity=29.8642533936652, Blast_Score=98, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17549921, Length=220, Percent_Identity=30, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=26.6932270916335, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17560910, Length=199, Percent_Identity=28.643216080402, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=269, Percent_Identity=28.996282527881, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=196, Percent_Identity=27.5510204081633, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=199, Percent_Identity=27.1356783919598, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI20129971, Length=279, Percent_Identity=30.1075268817204, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24653477, Length=279, Percent_Identity=30.1075268817204, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24654903, Length=261, Percent_Identity=23.3716475095785, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=29.6577946768061, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21357171, Length=197, Percent_Identity=32.48730964467, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19922422, Length=270, Percent_Identity=26.2962962962963, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=29.0196078431373, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24650670, Length=189, Percent_Identity=32.2751322751323, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 30648; Mature: 30517

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRDILYQDILYEVADRIATITLNRPDRMNAWTPVMEREVREAMETAAEDAEVRVIVLT
CCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEE
GAGRGFCAGADMQVLQTIDPSDVRRASSLPPFDMNRRADWQTRYAFYPAIPKPIIGMLNG
CCCCCCCCCCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCHHHHHHHCC
ATAGIGLVHALYCDVRFAADSAVFTTAFARRGLIAEHGISWMLPRIVGHANALDLLLSAR
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHCCHHHHHHHHHHH
RVSAEEALRIGLVNRLVPSEQLREQTYAYARDLADNVSPASMRVIKRQLYEVPFQTLAEA
HHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
TIEANREMVVSLGSDDFKEGVASFVEKRPPQFRSV
HHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STRDILYQDILYEVADRIATITLNRPDRMNAWTPVMEREVREAMETAAEDAEVRVIVLT
CCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEE
GAGRGFCAGADMQVLQTIDPSDVRRASSLPPFDMNRRADWQTRYAFYPAIPKPIIGMLNG
CCCCCCCCCCCHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHEEECCCCCHHHHHHHCC
ATAGIGLVHALYCDVRFAADSAVFTTAFARRGLIAEHGISWMLPRIVGHANALDLLLSAR
CHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCHHHHHHHHHCCHHHHHHHHHHH
RVSAEEALRIGLVNRLVPSEQLREQTYAYARDLADNVSPASMRVIKRQLYEVPFQTLAEA
HHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
TIEANREMVVSLGSDDFKEGVASFVEKRPPQFRSV
HHHCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA