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The map label for this gene is nudG [H]

Identifier: 86747156

GI number: 86747156

Start: 27426

End: 27839

Strand: Reverse

Name: nudG [H]

Synonym: RPB_0029

Alternate gene names: 86747156

Gene position: 27839-27426 (Counterclockwise)

Preceding gene: 86747157

Following gene: 86747153

Centisome position: 0.52

GC content: 66.18

Gene sequence:

>414_bases
ATGACGAAGTCTATCAAGCTCCTCCTCGTCGTCGCCGTCGCGCTGATCGACGCCGACAACCGCGTGCTGATCGCGCAGCG
GCCGAAGCACAAGCAGCTCGGCGGGTTGTGGGAATTTCCCGGCGGCAAGGTCGATCCCGGCGAGCGGCCGGAAGCCGCGC
TGATCCGCGAACTCGATGAGGAACTCGGCATCACGGTGAAAGAAGCCTGCCTGGCGCCGCTGACCTTCGCCAGCCATGCC
TATGAGGACTTTCACCTGCTGATGCCGCTGTATGTCTGCCGGCGCTGGGACGGGCAGGTGATGCCGCGCGAGGGCCAGGA
GCTGGCCTGGGTCCGCGCCAACAAGCTGCGCGACTACCCGATGCCGCCGGCCGATATCCCGCTGATCCCGCCGCTGATCG
AGCTGCTGATGTAG

Upstream 100 bases:

>100_bases
CCACGTCATTCCGGGGCGCGAGCGCAGCTCGCGAACCCGGAATCCATAACCCCTGCAGGGAGTATGGATTCCGGGCCCGC
GCCAAGAGGCGCGTCCCGGA

Downstream 100 bases:

>100_bases
TTCGTCATGCTGAGGCGCTCGCCCGCAGGGCGAGCCTCGAAGCATGCGCCACACGCGCGGCCTATCCTTCGAGGCTCGCT
ACGCTCGCGCCTCAGGATGA

Product: NUDIX hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 136

Protein sequence:

>137_residues
MTKSIKLLLVVAVALIDADNRVLIAQRPKHKQLGGLWEFPGGKVDPGERPEAALIRELDEELGITVKEACLAPLTFASHA
YEDFHLLMPLYVCRRWDGQVMPREGQELAWVRANKLRDYPMPPADIPLIPPLIELLM

Sequences:

>Translated_137_residues
MTKSIKLLLVVAVALIDADNRVLIAQRPKHKQLGGLWEFPGGKVDPGERPEAALIRELDEELGITVKEACLAPLTFASHA
YEDFHLLMPLYVCRRWDGQVMPREGQELAWVRANKLRDYPMPPADIPLIPPLIELLM
>Mature_136_residues
TKSIKLLLVVAVALIDADNRVLIAQRPKHKQLGGLWEFPGGKVDPGERPEAALIRELDEELGITVKEACLAPLTFASHAY
EDFHLLMPLYVCRRWDGQVMPREGQELAWVRANKLRDYPMPPADIPLIPPLIELLM

Specific function: Specific for pyrimidine substrates. Acts on 5-methyl- dCTP, CTP and dCTP in decreasing order [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1788056, Length=133, Percent_Identity=40.6015037593985, Blast_Score=86, Evalue=9e-19,
Organism=Escherichia coli, GI1786288, Length=130, Percent_Identity=36.1538461538462, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.-

Molecular weight: Translated: 15460; Mature: 15329

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSIKLLLVVAVALIDADNRVLIAQRPKHKQLGGLWEFPGGKVDPGERPEAALIRELDE
CCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHH
ELGITVKEACLAPLTFASHAYEDFHLLMPLYVCRRWDGQVMPREGQELAWVRANKLRDYP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHCCCCCCC
MPPADIPLIPPLIELLM
CCCCCCCCHHHHHHHHC
>Mature Secondary Structure 
TKSIKLLLVVAVALIDADNRVLIAQRPKHKQLGGLWEFPGGKVDPGERPEAALIRELDE
CCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHH
ELGITVKEACLAPLTFASHAYEDFHLLMPLYVCRRWDGQVMPREGQELAWVRANKLRDYP
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHCCCCCCC
MPPADIPLIPPLIELLM
CCCCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe; Mn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 11053429 [H]