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The map label for this gene is miaB [H]

Identifier: 86609143

GI number: 86609143

Start: 1758620

End: 1759954

Strand: Reverse

Name: miaB [H]

Synonym: CYB_1683

Alternate gene names: 86609143

Gene position: 1759954-1758620 (Counterclockwise)

Preceding gene: 86609147

Following gene: 86609141

Centisome position: 57.77

GC content: 61.95

Gene sequence:

>1335_bases
ATGGCTGCTCCCACCTATTACACCATCACCTTCGGCTGCCAAATGAACCGCGCCGATACCGAGCGCATGGCCGGGATCCT
GGAGTCCCTGGGGTATGTGGCCACCGAAGATGAGTTGCAGGCGGACTTAGTTCTCTACAACACCTGCACCATCCGCGACA
ATGCTGAGCAAAAGGTCTATTCCTACCTGGGCATCCAGGCGCAGCGCAAGCGCAAAAACCCGGCCATCAAGCTCATTGTG
GCCGGCTGTGTGGCCCAGCAGGAAGGGGAAAAATTGTTGCGCCGCGTCCCTGAGCTGGATCTGGTCATGGGCCCCCAGTA
CGTGAACCGCCTTGGGGATTTGCTGGCGCAGGTGGAAGCCGGAAACCAGGTGGTGGCCACCGATCCGGTTGAGATCCCAG
AAGACATCACCAAGCCCCGTCGCGACAGCCAGGTCACTGCTTGGATCAACGTTATCTACGGTTGCAACGAGCGCTGCACC
TACTGCATCGTGCCGCGGGTGCGAGGGCAAGAGCAGTCCCGCCAGCCCCAGGCCATCCGCGCCGAAATCGAAGACGTGGC
CAGAGCAGGCTATCGAGAGGTGACGTTGCTGGGGCAGAACATCGATGCCTACGGACGGGATCTGGATCCCAAAACCAACC
TGGCCAGCTTGTTGCGCTTCGTCCATTCGGTGGAAGGTATTGAACGGATCCGCTTTGCCACCAGCCACCCGCGCTACTTT
ACCGAGGAGCTGATCACCACCTGCGCCGAGCTGCCCAAAGTCTGCGAGCACTTCCACATTCCCTTCCAGGCGGGCAGCAA
CGAAGTGCTCAAGCGGATGCGGCGCGGCTACACCCACGAGCGCTATCGCCAGATTATCCAGCTCATCCGCCAATACATGC
CCGAAGCCGCCATCAGCGCCGATGCCATTGTCGGCTTCCCGGGAGAGACCGAAGCTCAGTTCCAAGAAACCCTGCAACTG
GTGCAGGACATTGGCTTTGACCAAGTGAACACTGCTGCCTACTCCCCCCGTCCCGGCACCCCTGCCGCCGAGTGGCCCGA
CCAGGTGCCTGAAGAGGAAAAAAGCGACCGCCTGCAACGGCTGAACCGACTGGTGGCCGAGGTGGCCGCCGCCCGCAGCG
CCCGACTCTTAGGCCAGGTGCAAGAGGTGCTGGTGGAAGGCCCCAACCCGAAAAACCCCCGCCAGGCGATGGGGCGTACC
CGCGGCAATCGCTTGGTGTTCTTTGAAGGGGATCCGGAGGAACTGCAGGGATCCCTGGTGCCGGTGCGCATTACCGCCAC
GCGAGCTTTTTCGCTGACCGGAGAGGCAGTTACCGTCCGCGCGAGCGGGCCATAG

Upstream 100 bases:

>100_bases
GGAACTTTTATCTAGACTAACCGTTGCAGTCTCAAACCCCTCAGCCATTTTAAGGATCCGCTAAGCTCGAAGATGGTGGT
TCTTTGTGCTGTTACTTCCC

Downstream 100 bases:

>100_bases
GATGAAGGCTCTCACAAACACAACTTTCCCCAGTTCCCTCTTGTCCGATTGCGCCGGCGGAGCGCCTCTCCAGGGAGCTG
TTTCGCTGAGAAAAACCATG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MAAPTYYTITFGCQMNRADTERMAGILESLGYVATEDELQADLVLYNTCTIRDNAEQKVYSYLGIQAQRKRKNPAIKLIV
AGCVAQQEGEKLLRRVPELDLVMGPQYVNRLGDLLAQVEAGNQVVATDPVEIPEDITKPRRDSQVTAWINVIYGCNERCT
YCIVPRVRGQEQSRQPQAIRAEIEDVARAGYREVTLLGQNIDAYGRDLDPKTNLASLLRFVHSVEGIERIRFATSHPRYF
TEELITTCAELPKVCEHFHIPFQAGSNEVLKRMRRGYTHERYRQIIQLIRQYMPEAAISADAIVGFPGETEAQFQETLQL
VQDIGFDQVNTAAYSPRPGTPAAEWPDQVPEEEKSDRLQRLNRLVAEVAAARSARLLGQVQEVLVEGPNPKNPRQAMGRT
RGNRLVFFEGDPEELQGSLVPVRITATRAFSLTGEAVTVRASGP

Sequences:

>Translated_444_residues
MAAPTYYTITFGCQMNRADTERMAGILESLGYVATEDELQADLVLYNTCTIRDNAEQKVYSYLGIQAQRKRKNPAIKLIV
AGCVAQQEGEKLLRRVPELDLVMGPQYVNRLGDLLAQVEAGNQVVATDPVEIPEDITKPRRDSQVTAWINVIYGCNERCT
YCIVPRVRGQEQSRQPQAIRAEIEDVARAGYREVTLLGQNIDAYGRDLDPKTNLASLLRFVHSVEGIERIRFATSHPRYF
TEELITTCAELPKVCEHFHIPFQAGSNEVLKRMRRGYTHERYRQIIQLIRQYMPEAAISADAIVGFPGETEAQFQETLQL
VQDIGFDQVNTAAYSPRPGTPAAEWPDQVPEEEKSDRLQRLNRLVAEVAAARSARLLGQVQEVLVEGPNPKNPRQAMGRT
RGNRLVFFEGDPEELQGSLVPVRITATRAFSLTGEAVTVRASGP
>Mature_443_residues
AAPTYYTITFGCQMNRADTERMAGILESLGYVATEDELQADLVLYNTCTIRDNAEQKVYSYLGIQAQRKRKNPAIKLIVA
GCVAQQEGEKLLRRVPELDLVMGPQYVNRLGDLLAQVEAGNQVVATDPVEIPEDITKPRRDSQVTAWINVIYGCNERCTY
CIVPRVRGQEQSRQPQAIRAEIEDVARAGYREVTLLGQNIDAYGRDLDPKTNLASLLRFVHSVEGIERIRFATSHPRYFT
EELITTCAELPKVCEHFHIPFQAGSNEVLKRMRRGYTHERYRQIIQLIRQYMPEAAISADAIVGFPGETEAQFQETLQLV
QDIGFDQVNTAAYSPRPGTPAAEWPDQVPEEEKSDRLQRLNRLVAEVAAARSARLLGQVQEVLVEGPNPKNPRQAMGRTR
GNRLVFFEGDPEELQGSLVPVRITATRAFSLTGEAVTVRASGP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=473, Percent_Identity=32.5581395348837, Blast_Score=248, Evalue=9e-66,
Organism=Homo sapiens, GI28872782, Length=473, Percent_Identity=32.5581395348837, Blast_Score=247, Evalue=2e-65,
Organism=Homo sapiens, GI93277076, Length=392, Percent_Identity=30.3571428571429, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1786882, Length=436, Percent_Identity=42.2018348623853, Blast_Score=338, Evalue=5e-94,
Organism=Escherichia coli, GI1787057, Length=454, Percent_Identity=28.8546255506608, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=461, Percent_Identity=32.1041214750542, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI71996771, Length=375, Percent_Identity=26.6666666666667, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356207, Length=428, Percent_Identity=35.981308411215, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI19922432, Length=452, Percent_Identity=28.7610619469027, Blast_Score=169, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49774; Mature: 49643

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPTYYTITFGCQMNRADTERMAGILESLGYVATEDELQADLVLYNTCTIRDNAEQKVY
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCHHHHHH
SYLGIQAQRKRKNPAIKLIVAGCVAQQEGEKLLRRVPELDLVMGPQYVNRLGDLLAQVEA
HHHCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHC
GNQVVATDPVEIPEDITKPRRDSQVTAWINVIYGCNERCTYCIVPRVRGQEQSRQPQAIR
CCEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHH
AEIEDVARAGYREVTLLGQNIDAYGRDLDPKTNLASLLRFVHSVEGIERIRFATSHPRYF
HHHHHHHHCCCCEEEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
TEELITTCAELPKVCEHFHIPFQAGSNEVLKRMRRGYTHERYRQIIQLIRQYMPEAAISA
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHCCC
DAIVGFPGETEAQFQETLQLVQDIGFDQVNTAAYSPRPGTPAAEWPDQVPEEEKSDRLQR
CCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LNRLVAEVAAARSARLLGQVQEVLVEGPNPKNPRQAMGRTRGNRLVFFEGDPEELQGSLV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCHHHHCCCEE
PVRITATRAFSLTGEAVTVRASGP
EEEEEEEEEEEECCCEEEEEECCC
>Mature Secondary Structure 
AAPTYYTITFGCQMNRADTERMAGILESLGYVATEDELQADLVLYNTCTIRDNAEQKVY
CCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCHHHHHH
SYLGIQAQRKRKNPAIKLIVAGCVAQQEGEKLLRRVPELDLVMGPQYVNRLGDLLAQVEA
HHHCCHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHHC
GNQVVATDPVEIPEDITKPRRDSQVTAWINVIYGCNERCTYCIVPRVRGQEQSRQPQAIR
CCEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHH
AEIEDVARAGYREVTLLGQNIDAYGRDLDPKTNLASLLRFVHSVEGIERIRFATSHPRYF
HHHHHHHHCCCCEEEEECCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHH
TEELITTCAELPKVCEHFHIPFQAGSNEVLKRMRRGYTHERYRQIIQLIRQYMPEAAISA
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHCCC
DAIVGFPGETEAQFQETLQLVQDIGFDQVNTAAYSPRPGTPAAEWPDQVPEEEKSDRLQR
CCEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
LNRLVAEVAAARSARLLGQVQEVLVEGPNPKNPRQAMGRTRGNRLVFFEGDPEELQGSLV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEECCCHHHHCCCEE
PVRITATRAFSLTGEAVTVRASGP
EEEEEEEEEEEECCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA