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The map label for this gene is btuB [H]

Identifier: 86608804

GI number: 86608804

Start: 1391212

End: 1393182

Strand: Direct

Name: btuB [H]

Synonym: CYB_1330

Alternate gene names: 86608804

Gene position: 1391212-1393182 (Clockwise)

Preceding gene: 86608803

Following gene: 86608805

Centisome position: 45.66

GC content: 56.47

Gene sequence:

>1971_bases
ATGAATCCCATTCTAGGTCTCAGCCTGGCGCTGGGGGGTCTTGCTTTGGGGATCCCAGCGTGGGCGCAGCCGGCGGCCTC
TTCACCTTCAGCTCAAGAGGTTTTGGAAACCGGTGTTAGCGCCGAGATTTTAAATCAACCCGTCACCACACCTTTTCGCC
GTCCTGGTTTGCTGCGGGATTCTTCCCGCCCGGTTTACGTCATTACCCGCCAGCAAATTCGAGAGCAAGGGGCAAGAACG
GTTCAGGAGGCCCTGCGCTACCTGCCCGGGATCCTCAGCGACGGCACGGCGGGAACCCAGTTGGGAGCCCTTAGCTCCCA
GTTTATGCGGGGAGGACGAACGGCCCAGGTGTTGGTGCTCTTGGATGGCCGCCCCATTAACGACCTGGGCTTTTTTGGCG
GGTTTGATTTGTCCCAGATCACTACAGATGTGGTCGAGCGTATTGAGATCTCTCCAGGTGGGGGCTCCACTTTGTACGGA
TCCGACGCGGTTGGCGGGGTCATGAACATCGTAACCCTGGAGCCAAAGCCGGGATCCGAGACCGTGCTGGAAGCGGCTAT
CGGCAGCTACGGCCTCAACGAGCAAGGGATCCAAAACCGCGGCCAAGCGCAGGATCTGGGCTGGGTGGTCAGCTATCGCC
GTCTGCAGAGCAACAACGACTTTCCTTTTGAAATTAGGCGTTTGGGCAAAGCCGGCAGGCGGGAAAACTCGGCGGTGGAG
TACCACAACCTACAAGCCAAGCTCACCTGGCAATTGGCAGAAGGCCAAGTCCTAACCGGAAATGCCCTTTATCTCAACAA
AGCGTTTCAGGTGCCTGGCAGCACCGAGTTTCCCTCCCCCGATGCAGTGCAAAACACCAACGACCTGCTGTTAGATCTCA
GTTGGCTGTGGGATCAAGAGAGGGGATCCCTGCAAGCCAGGGTATTTTTTGACGACCTCAACTATCGCTTCAGCGCCCCA
CAAAGCCAGGGATCCCGGGATGAGATCCTGCGAACGGGGTGGGGATCCCAACTGCAATACACCTGGAAGGCTGCTGAAAA
CCTGGAGCTTACTCTAGGAGCGGATTACCGATCCATTTCAGCTCGCAACACCACCTTCTCCTTTGCCAGGGGATCCACCA
ACGTCAACTATGACAATACTGTGAATCAGGGGGCATTCTTCGCCCGCCTGGATTACAGGCCACAGCCCAACTTTTATCTC
AACTTAGGTGTTCGCCAAGATTCGAGCAATCTGGTCAACGGTTCGGCCACCTCTCCTGCCGCAGGGATCCGCTGGGTTTT
GGCAGAAGGCACCACCCTGAGGGCCAATTATGCCGGCAGCTTTCGCACCCCCTTGATCTCAAACCTGTTCTTTCGGCTGC
GCGGCTTTTTTGAAGTCGACGGCAACCCCAATTTGCGACCGGAGCGGGGAGAGAGTTTTGACATTGGCCTGGATCAGGCT
TTGGGAGATTTTGGCCTACTGCGCTTGACCTTTGCCCATAACCGCATCCGCGATGGCATTACCTTTGTCAGCACTGGCCC
GCGCAGGGGAACCTACGAAAACATTGAGCTCGTGGAATACACGGGCTTGGAAGCAGCCCTGGATTTGCGATTGGGGGAAA
ACTGGACTCTTTTTGCCAACTACACCCTGAACGATCCCATCATCCGCTCGGCGCTGGATCCCAAAGCGGTTGGCAAACAG
CTAGCCTTCGTGGGAGCAGACAGCTTCAACCTGGGCCTGAGCTACCGCGATGCAGAGGATCGGTATGCCTCAGTTTTCCT
GCATCACATTGGCACTTTCTTTACCAACAACGCCAATACCCAATCTCTGCCCGGCTACACCAACCTGGCTCTGCGGGGAG
GGATCCCAGTAGCAGAAAATCTCGCTCTAGAAGTGGGGGTTGATAACCTGCTGGATGAGCAATATGAGGTTTTCCCCGGC
TACCCCGGAGTGGGTCGCACCGTTCGGCTTAATTTAAGGGGCCAATTTTAG

Upstream 100 bases:

>100_bases
GGGCTTAGGCTAGACCTATGCCCTCAGTCAGGATGCCCGCCTGCGGTTTTGGCTCGCATTTCTTCCGCGTCGCACGGAAT
CAGGAGCTTCAAGAAAGAAA

Downstream 100 bases:

>100_bases
AGCGACTTGAGGCTGACTTTTAGCTGTCTAACTCACTGCTGAATCCTTGGCCAAGACATTGAGATCGTCTTTGCCTCTTC
TCACACCCTTTGCTTGGACA

Product: outer membrane receptor (OMR) family protein

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MNPILGLSLALGGLALGIPAWAQPAASSPSAQEVLETGVSAEILNQPVTTPFRRPGLLRDSSRPVYVITRQQIREQGART
VQEALRYLPGILSDGTAGTQLGALSSQFMRGGRTAQVLVLLDGRPINDLGFFGGFDLSQITTDVVERIEISPGGGSTLYG
SDAVGGVMNIVTLEPKPGSETVLEAAIGSYGLNEQGIQNRGQAQDLGWVVSYRRLQSNNDFPFEIRRLGKAGRRENSAVE
YHNLQAKLTWQLAEGQVLTGNALYLNKAFQVPGSTEFPSPDAVQNTNDLLLDLSWLWDQERGSLQARVFFDDLNYRFSAP
QSQGSRDEILRTGWGSQLQYTWKAAENLELTLGADYRSISARNTTFSFARGSTNVNYDNTVNQGAFFARLDYRPQPNFYL
NLGVRQDSSNLVNGSATSPAAGIRWVLAEGTTLRANYAGSFRTPLISNLFFRLRGFFEVDGNPNLRPERGESFDIGLDQA
LGDFGLLRLTFAHNRIRDGITFVSTGPRRGTYENIELVEYTGLEAALDLRLGENWTLFANYTLNDPIIRSALDPKAVGKQ
LAFVGADSFNLGLSYRDAEDRYASVFLHHIGTFFTNNANTQSLPGYTNLALRGGIPVAENLALEVGVDNLLDEQYEVFPG
YPGVGRTVRLNLRGQF

Sequences:

>Translated_656_residues
MNPILGLSLALGGLALGIPAWAQPAASSPSAQEVLETGVSAEILNQPVTTPFRRPGLLRDSSRPVYVITRQQIREQGART
VQEALRYLPGILSDGTAGTQLGALSSQFMRGGRTAQVLVLLDGRPINDLGFFGGFDLSQITTDVVERIEISPGGGSTLYG
SDAVGGVMNIVTLEPKPGSETVLEAAIGSYGLNEQGIQNRGQAQDLGWVVSYRRLQSNNDFPFEIRRLGKAGRRENSAVE
YHNLQAKLTWQLAEGQVLTGNALYLNKAFQVPGSTEFPSPDAVQNTNDLLLDLSWLWDQERGSLQARVFFDDLNYRFSAP
QSQGSRDEILRTGWGSQLQYTWKAAENLELTLGADYRSISARNTTFSFARGSTNVNYDNTVNQGAFFARLDYRPQPNFYL
NLGVRQDSSNLVNGSATSPAAGIRWVLAEGTTLRANYAGSFRTPLISNLFFRLRGFFEVDGNPNLRPERGESFDIGLDQA
LGDFGLLRLTFAHNRIRDGITFVSTGPRRGTYENIELVEYTGLEAALDLRLGENWTLFANYTLNDPIIRSALDPKAVGKQ
LAFVGADSFNLGLSYRDAEDRYASVFLHHIGTFFTNNANTQSLPGYTNLALRGGIPVAENLALEVGVDNLLDEQYEVFPG
YPGVGRTVRLNLRGQF
>Mature_656_residues
MNPILGLSLALGGLALGIPAWAQPAASSPSAQEVLETGVSAEILNQPVTTPFRRPGLLRDSSRPVYVITRQQIREQGART
VQEALRYLPGILSDGTAGTQLGALSSQFMRGGRTAQVLVLLDGRPINDLGFFGGFDLSQITTDVVERIEISPGGGSTLYG
SDAVGGVMNIVTLEPKPGSETVLEAAIGSYGLNEQGIQNRGQAQDLGWVVSYRRLQSNNDFPFEIRRLGKAGRRENSAVE
YHNLQAKLTWQLAEGQVLTGNALYLNKAFQVPGSTEFPSPDAVQNTNDLLLDLSWLWDQERGSLQARVFFDDLNYRFSAP
QSQGSRDEILRTGWGSQLQYTWKAAENLELTLGADYRSISARNTTFSFARGSTNVNYDNTVNQGAFFARLDYRPQPNFYL
NLGVRQDSSNLVNGSATSPAAGIRWVLAEGTTLRANYAGSFRTPLISNLFFRLRGFFEVDGNPNLRPERGESFDIGLDQA
LGDFGLLRLTFAHNRIRDGITFVSTGPRRGTYENIELVEYTGLEAALDLRLGENWTLFANYTLNDPIIRSALDPKAVGKQ
LAFVGADSFNLGLSYRDAEDRYASVFLHHIGTFFTNNANTQSLPGYTNLALRGGIPVAENLALEVGVDNLLDEQYEVFPG
YPGVGRTVRLNLRGQF

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=675, Percent_Identity=25.037037037037, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1788478, Length=175, Percent_Identity=28, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1787723, Length=222, Percent_Identity=25.6756756756757, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010916
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 71938; Mature: 71938

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPILGLSLALGGLALGIPAWAQPAASSPSAQEVLETGVSAEILNQPVTTPFRRPGLLRD
CCCCEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCHHHHCCCCCCCCCCCCCCCC
SSRPVYVITRQQIREQGARTVQEALRYLPGILSDGTAGTQLGALSSQFMRGGRTAQVLVL
CCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEE
LDGRPINDLGFFGGFDLSQITTDVVERIEISPGGGSTLYGSDAVGGVMNIVTLEPKPGSE
ECCCCCHHHCCCCCCCHHHHHHHHHHHEEECCCCCCEEECCCCCCCEEEEEEECCCCCHH
TVLEAAIGSYGLNEQGIQNRGQAQDLGWVVSYRRLQSNNDFPFEIRRLGKAGRRENSAVE
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEE
YHNLQAKLTWQLAEGQVLTGNALYLNKAFQVPGSTEFPSPDAVQNTNDLLLDLSWLWDQE
EECCEEEEEEEEECCEEEECCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEHHCCCC
RGSLQARVFFDDLNYRFSAPQSQGSRDEILRTGWGSQLQYTWKAAENLELTLGADYRSIS
CCCEEEEEEEECCCCEECCCCCCCCHHHHHHCCCCCCEEEEEECCCCEEEEECCCCEECC
ARNTTFSFARGSTNVNYDNTVNQGAFFARLDYRPQPNFYLNLGVRQDSSNLVNGSATSPA
CCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCC
AGIRWVLAEGTTLRANYAGSFRTPLISNLFFRLRGFFEVDGNPNLRPERGESFDIGLDQA
CCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCEECCCHHHH
LGDFGLLRLTFAHNRIRDGITFVSTGPRRGTYENIELVEYTGLEAALDLRLGENWTLFAN
HCCCCEEEEEEHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCCEEEEEEECCCEEEEEE
YTLNDPIIRSALDPKAVGKQLAFVGADSFNLGLSYRDAEDRYASVFLHHIGTFFTNNANT
EECCCHHHHHCCCHHHHCCEEEEEECCCEECCEEECCCHHHHHHHHHHHHHHHEECCCCC
QSLPGYTNLALRGGIPVAENLALEVGVDNLLDEQYEVFPGYPGVGRTVRLNLRGQF
CCCCCCCCEEEECCCCCHHCEEEEECCHHHHCCHHHCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MNPILGLSLALGGLALGIPAWAQPAASSPSAQEVLETGVSAEILNQPVTTPFRRPGLLRD
CCCCEEHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCHHHHCCCCCCCCCCCCCCCC
SSRPVYVITRQQIREQGARTVQEALRYLPGILSDGTAGTQLGALSSQFMRGGRTAQVLVL
CCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEE
LDGRPINDLGFFGGFDLSQITTDVVERIEISPGGGSTLYGSDAVGGVMNIVTLEPKPGSE
ECCCCCHHHCCCCCCCHHHHHHHHHHHEEECCCCCCEEECCCCCCCEEEEEEECCCCCHH
TVLEAAIGSYGLNEQGIQNRGQAQDLGWVVSYRRLQSNNDFPFEIRRLGKAGRRENSAVE
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCEE
YHNLQAKLTWQLAEGQVLTGNALYLNKAFQVPGSTEFPSPDAVQNTNDLLLDLSWLWDQE
EECCEEEEEEEEECCEEEECCEEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEEHHCCCC
RGSLQARVFFDDLNYRFSAPQSQGSRDEILRTGWGSQLQYTWKAAENLELTLGADYRSIS
CCCEEEEEEEECCCCEECCCCCCCCHHHHHHCCCCCCEEEEEECCCCEEEEECCCCEECC
ARNTTFSFARGSTNVNYDNTVNQGAFFARLDYRPQPNFYLNLGVRQDSSNLVNGSATSPA
CCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCC
AGIRWVLAEGTTLRANYAGSFRTPLISNLFFRLRGFFEVDGNPNLRPERGESFDIGLDQA
CCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCEECCCHHHH
LGDFGLLRLTFAHNRIRDGITFVSTGPRRGTYENIELVEYTGLEAALDLRLGENWTLFAN
HCCCCEEEEEEHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCCEEEEEEECCCEEEEEE
YTLNDPIIRSALDPKAVGKQLAFVGADSFNLGLSYRDAEDRYASVFLHHIGTFFTNNANT
EECCCHHHHHCCCHHHHCCEEEEEECCCEECCEEECCCHHHHHHHHHHHHHHHEECCCCC
QSLPGYTNLALRGGIPVAENLALEVGVDNLLDEQYEVFPGYPGVGRTVRLNLRGQF
CCCCCCCCEEEECCCCCHHCEEEEECCHHHHCCHHHCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA