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The map label for this gene is mrdA [H]

Identifier: 86608722

GI number: 86608722

Start: 1293729

End: 1294613

Strand: Direct

Name: mrdA [H]

Synonym: CYB_1246

Alternate gene names: 86608722

Gene position: 1293729-1294613 (Clockwise)

Preceding gene: 86608711

Following gene: 86608723

Centisome position: 42.46

GC content: 62.82

Gene sequence:

>885_bases
ATGAACCGTCGCACCTGTGGAGCCTGGCTATTGGGTTCTGCTTTGACCTGGGGCTTGCAGGGGAAATCTGCAGGGCTGCC
CCATCCGAGCGATTTTGCCGCCGTCCTCAACCTGAACACCGGTCGTTTAACAAGGGTTTGGCAGCCGGAGCAGGCTTCCA
GAATCAGGGCCAGCCCTGGCTCAACCGTCAAATGCGCCGTTGCCGCCGCTGCTCTGCAGACTGGGATCAGCTCTGGGGAG
CGCTTGATCCACTGTCCGGGTTGGTGGGATCCGCCGCCGCAACTGGGGTTGGGCCGCCTACCCTGCTGGGATCCCCGCGG
ACATGGAACCTTGAACTTGCGCCAAGCCCTGGCCCACTCTTGCAACGTGCATTTTTACCAGTTGGGCTGGGAGCTGGGGG
CCAATACCCTGGTGGAGTGGCTGCGGCGCTTCGGCCTAGAGAGTGCGCAGAATACGGCTCCGGCTCCGCTCTTGGCGACC
GGTCTGGCTGGGCCGGTGGATCCCTGGGAGCTGTTGGTTTATGGGGGCGTGATTGCCCGCCGAGGTGTACCCCTTTCTGG
CGAGGTCTCTCTGCCCGCTTTCTCTTTACCGGAGTGGATTTGGGAGGCTCTCCATGCTGGCATGAGCCTGGCGGCAACAG
CGGGCACCTGTCGCGGCATTGCCCCAGAAGGGATCCCAGTGGCAGCCAAGTCTGGCACCATCCTCAATCCTCAACGACGA
GGACGACCAATTCTCTGGGCAGAAGATTTTCAAGCCTGGTTGCTGGCCTTTTGGCCCCTGCCGGATCCCAACTGGACGCT
GGTGCTGCACCTGCATCAGGGCAAAGCCTATGAGGCGGCCATTCCCCTGGCGCGGCAGATCATCGCCGCCATCGGCAGCG
GGTGA

Upstream 100 bases:

>100_bases
CTGAACTCAGATTACCCTAGTCCGTGAGAAGGCTGCGATTTCCGAATTTTTGTAACAGGGATCCGCTTTTGTCTGGCTGG
GTTTTCGGGGCCGACAGCAG

Downstream 100 bases:

>100_bases
CCACCTTGCTTGCGATCATGAAGAAATGCAAACTATACTGAAGGTCAAGTGCCGTTCTCGTTTTCGTTCTTTACGGATCC
CACTCTCTGTCGTTCCCATT

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MNRRTCGAWLLGSALTWGLQGKSAGLPHPSDFAAVLNLNTGRLTRVWQPEQASRIRASPGSTVKCAVAAAALQTGISSGE
RLIHCPGWWDPPPQLGLGRLPCWDPRGHGTLNLRQALAHSCNVHFYQLGWELGANTLVEWLRRFGLESAQNTAPAPLLAT
GLAGPVDPWELLVYGGVIARRGVPLSGEVSLPAFSLPEWIWEALHAGMSLAATAGTCRGIAPEGIPVAAKSGTILNPQRR
GRPILWAEDFQAWLLAFWPLPDPNWTLVLHLHQGKAYEAAIPLARQIIAAIGSG

Sequences:

>Translated_294_residues
MNRRTCGAWLLGSALTWGLQGKSAGLPHPSDFAAVLNLNTGRLTRVWQPEQASRIRASPGSTVKCAVAAAALQTGISSGE
RLIHCPGWWDPPPQLGLGRLPCWDPRGHGTLNLRQALAHSCNVHFYQLGWELGANTLVEWLRRFGLESAQNTAPAPLLAT
GLAGPVDPWELLVYGGVIARRGVPLSGEVSLPAFSLPEWIWEALHAGMSLAATAGTCRGIAPEGIPVAAKSGTILNPQRR
GRPILWAEDFQAWLLAFWPLPDPNWTLVLHLHQGKAYEAAIPLARQIIAAIGSG
>Mature_294_residues
MNRRTCGAWLLGSALTWGLQGKSAGLPHPSDFAAVLNLNTGRLTRVWQPEQASRIRASPGSTVKCAVAAAALQTGISSGE
RLIHCPGWWDPPPQLGLGRLPCWDPRGHGTLNLRQALAHSCNVHFYQLGWELGANTLVEWLRRFGLESAQNTAPAPLLAT
GLAGPVDPWELLVYGGVIARRGVPLSGEVSLPAFSLPEWIWEALHAGMSLAATAGTCRGIAPEGIPVAAKSGTILNPQRR
GRPILWAEDFQAWLLAFWPLPDPNWTLVLHLHQGKAYEAAIPLARQIIAAIGSG

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=117, Percent_Identity=35.042735042735, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 31568; Mature: 31568

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRRTCGAWLLGSALTWGLQGKSAGLPHPSDFAAVLNLNTGRLTRVWQPEQASRIRASPG
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCHHHHEECCCC
STVKCAVAAAALQTGISSGERLIHCPGWWDPPPQLGLGRLPCWDPRGHGTLNLRQALAHS
CCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHH
CNVHFYQLGWELGANTLVEWLRRFGLESAQNTAPAPLLATGLAGPVDPWELLVYGGVIAR
CCEEEEEECHHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHCCHHHH
RGVPLSGEVSLPAFSLPEWIWEALHAGMSLAATAGTCRGIAPEGIPVAAKSGTILNPQRR
CCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEECCCCEECHHHC
GRPILWAEDFQAWLLAFWPLPDPNWTLVLHLHQGKAYEAAIPLARQIIAAIGSG
CCEEEEHHHHHEEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNRRTCGAWLLGSALTWGLQGKSAGLPHPSDFAAVLNLNTGRLTRVWQPEQASRIRASPG
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCHHHHEECCCC
STVKCAVAAAALQTGISSGERLIHCPGWWDPPPQLGLGRLPCWDPRGHGTLNLRQALAHS
CCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHH
CNVHFYQLGWELGANTLVEWLRRFGLESAQNTAPAPLLATGLAGPVDPWELLVYGGVIAR
CCEEEEEECHHHCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHCCHHHH
RGVPLSGEVSLPAFSLPEWIWEALHAGMSLAATAGTCRGIAPEGIPVAAKSGTILNPQRR
CCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEECCCCEECHHHC
GRPILWAEDFQAWLLAFWPLPDPNWTLVLHLHQGKAYEAAIPLARQIIAAIGSG
CCEEEEHHHHHEEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]