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The map label for this gene is murE [H]

Identifier: 86608711

GI number: 86608711

Start: 1277744

End: 1279276

Strand: Direct

Name: murE [H]

Synonym: CYB_1235

Alternate gene names: 86608711

Gene position: 1277744-1279276 (Clockwise)

Preceding gene: 86608710

Following gene: 86608722

Centisome position: 41.94

GC content: 64.71

Gene sequence:

>1533_bases
ATGGAAACTGCTGGGATCACTTTGTCCAAATTGCTCCACCAAGCTGGGATCCAGCCTCAATTGGCCCAGGGATCCCTGGA
GGGGGTGCAGGTCTGGGGCCTCTGTGCCGACTCTCGCCAGGTACAGCCGGGGGATCTCTTCTTGGGGATGCCGGGATCCC
GCGTGGATGGAGGTAGCTTCGTGGCCCAAGCCCTGGCGGCAGGGGCAGTGGCCGCCCTGATCTCCCAGGAGGCGTGGGAG
AGCGTCAGCGGAGAGGCTGGATGGGGAAGTCAGCCCGTCCTGGTTTTGCCTCGCGCCAGCCTGAACAGGGCCGTGGCCCA
GGTGGCGGCAGCTTTCTATGGGTTTCCCGCCCGCCAACTGACCTTGGTGGGGGTGACGGGCACCAATGGCAAAACCACCA
CCACCCACTTAATCGAACATTTGGTGCAAGCTGCCGGCCGCTCCGCGGCTTTGCTGGGCACTCTGTACAACCGCTGGCCT
GGCTATAGCGAAGTGGCCTCCCACACCACCCTTTTTCCGCCTGAACTGCAGCGCAGCCTCAGGGAAGCCCAGAGAGCCGG
GGCGGAGGTGGCGGTGATGGAGGTGAGCTCCCATGCTCTCGCTCAAGATCGGGTGTGGGGATGTTCCTTTGCCGCTGCCG
TCTGGACCAACCTTACCCAGGATCACCTGGATTTCCACGGCAGCATGGAGAACTACTGGCAGGCCAAGGCCACCCTCTTC
CGACAGGAGTATCTCCAAGGGCGGGCGGTGATCAACGCCGACGATGCTGGGGGACGGCGGCTCTTGCAAGCCATTCGGCC
CGATCTGCAGCCCTGGGCCTACTCGCTGCAGCCCCTGCAGGGGATCCCCGGCCTCTGGCCCAGCAACGTTCAATTGCACC
CCAGCGGCTTACAGGCGACGCTCCACACCCCCTCTGGCGCCTTTGCCGTGGCAGCCCCCCTGGTGGGATCCTTCAACCTG
GCCAACCTGCTGGCGGCGGTGGGGGTAGCCCTGCACTTGGGGATCCCTGTCGAGATCATCCAGTCGGCCCTGCCCCACTT
TCCGGGCGTACCGGGGCGGATGGAGCGGGTGACCCTGCCGGGGCAGGATATCGCGGTGATTGTGGACTATGCCCACACCC
CCGACGGGCTGGAAAACCTGCTCAAGGCCCTGCGCTCCCAGGTGCAAGGTCAGCTTATCTGTGTCTTTGGCTGTGGGGGG
GATCGGGATCGGGGCAAACGACCGCAGATGGGCCGCATTGCCGCCACCTGGGCCGATCAGGTGATTGTTACTTCCGACAA
CCCCCGCACCGAAGATCCCCAGCAGATCCTGCGGGATATTCTGGCCGGGATCCCTGGATCCCCCAGGGCGGTGGAGGTGG
ATCGGCGGCAGGCCATTCGCCTGGCCATCCTCGCAGCCCAGCCAGGGGATACGGTGGTCATTGCCGGCAAAGGCCATGAG
GACTACCAGATCCTGGGCACCGAGAAGATTCACTTCGACGACCGAGAAGAAGCCCGCGCTGCCCTGGCCCTGAGGGAAAA
AAGCCGGGGGTGA

Upstream 100 bases:

>100_bases
TCTGTGGGCCAAGCAAAAGCTGGAGGAAGCCCTGGCCTAATTTGACGGCGGCAATCCCTGGCCGCTACCCTAACCGGCAA
AGCTGTGGGGAGTGGCAACG

Downstream 100 bases:

>100_bases
GGCTAACGGGAGCGGTGCTGCAGCATCAGCTCCACGAACTCGCGGAAGAGGTGATCGGCGTCGTGGGGGCCGGGGCTGGC
TTCCGGGTGATACTGCACGG

Product: UDP-N-acetylmuramyl-tripeptide synthetase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
METAGITLSKLLHQAGIQPQLAQGSLEGVQVWGLCADSRQVQPGDLFLGMPGSRVDGGSFVAQALAAGAVAALISQEAWE
SVSGEAGWGSQPVLVLPRASLNRAVAQVAAAFYGFPARQLTLVGVTGTNGKTTTTHLIEHLVQAAGRSAALLGTLYNRWP
GYSEVASHTTLFPPELQRSLREAQRAGAEVAVMEVSSHALAQDRVWGCSFAAAVWTNLTQDHLDFHGSMENYWQAKATLF
RQEYLQGRAVINADDAGGRRLLQAIRPDLQPWAYSLQPLQGIPGLWPSNVQLHPSGLQATLHTPSGAFAVAAPLVGSFNL
ANLLAAVGVALHLGIPVEIIQSALPHFPGVPGRMERVTLPGQDIAVIVDYAHTPDGLENLLKALRSQVQGQLICVFGCGG
DRDRGKRPQMGRIAATWADQVIVTSDNPRTEDPQQILRDILAGIPGSPRAVEVDRRQAIRLAILAAQPGDTVVIAGKGHE
DYQILGTEKIHFDDREEARAALALREKSRG

Sequences:

>Translated_510_residues
METAGITLSKLLHQAGIQPQLAQGSLEGVQVWGLCADSRQVQPGDLFLGMPGSRVDGGSFVAQALAAGAVAALISQEAWE
SVSGEAGWGSQPVLVLPRASLNRAVAQVAAAFYGFPARQLTLVGVTGTNGKTTTTHLIEHLVQAAGRSAALLGTLYNRWP
GYSEVASHTTLFPPELQRSLREAQRAGAEVAVMEVSSHALAQDRVWGCSFAAAVWTNLTQDHLDFHGSMENYWQAKATLF
RQEYLQGRAVINADDAGGRRLLQAIRPDLQPWAYSLQPLQGIPGLWPSNVQLHPSGLQATLHTPSGAFAVAAPLVGSFNL
ANLLAAVGVALHLGIPVEIIQSALPHFPGVPGRMERVTLPGQDIAVIVDYAHTPDGLENLLKALRSQVQGQLICVFGCGG
DRDRGKRPQMGRIAATWADQVIVTSDNPRTEDPQQILRDILAGIPGSPRAVEVDRRQAIRLAILAAQPGDTVVIAGKGHE
DYQILGTEKIHFDDREEARAALALREKSRG
>Mature_510_residues
METAGITLSKLLHQAGIQPQLAQGSLEGVQVWGLCADSRQVQPGDLFLGMPGSRVDGGSFVAQALAAGAVAALISQEAWE
SVSGEAGWGSQPVLVLPRASLNRAVAQVAAAFYGFPARQLTLVGVTGTNGKTTTTHLIEHLVQAAGRSAALLGTLYNRWP
GYSEVASHTTLFPPELQRSLREAQRAGAEVAVMEVSSHALAQDRVWGCSFAAAVWTNLTQDHLDFHGSMENYWQAKATLF
RQEYLQGRAVINADDAGGRRLLQAIRPDLQPWAYSLQPLQGIPGLWPSNVQLHPSGLQATLHTPSGAFAVAAPLVGSFNL
ANLLAAVGVALHLGIPVEIIQSALPHFPGVPGRMERVTLPGQDIAVIVDYAHTPDGLENLLKALRSQVQGQLICVFGCGG
DRDRGKRPQMGRIAATWADQVIVTSDNPRTEDPQQILRDILAGIPGSPRAVEVDRRQAIRLAILAAQPGDTVVIAGKGHE
DYQILGTEKIHFDDREEARAALALREKSRG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=464, Percent_Identity=43.1034482758621, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1786274, Length=357, Percent_Identity=27.1708683473389, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54483; Mature: 54483

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METAGITLSKLLHQAGIQPQLAQGSLEGVQVWGLCADSRQVQPGDLFLGMPGSRVDGGSF
CCCCCHHHHHHHHHHCCCCCHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCHHH
VAQALAAGAVAALISQEAWESVSGEAGWGSQPVLVLPRASLNRAVAQVAAAFYGFPARQL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEE
TLVGVTGTNGKTTTTHLIEHLVQAAGRSAALLGTLYNRWPGYSEVASHTTLFPPELQRSL
EEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHH
REAQRAGAEVAVMEVSSHALAQDRVWGCSFAAAVWTNLTQDHLDFHGSMENYWQAKATLF
HHHHHCCCEEEEEECCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHH
RQEYLQGRAVINADDAGGRRLLQAIRPDLQPWAYSLQPLQGIPGLWPSNVQLHPSGLQAT
HHHHHCCCEEEECCCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCEECCCCCEEE
LHTPSGAFAVAAPLVGSFNLANLLAAVGVALHLGIPVEIIQSALPHFPGVPGRMERVTLP
EECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEEECC
GQDIAVIVDYAHTPDGLENLLKALRSQVQGQLICVFGCGGDRDRGKRPQMGRIAATWADQ
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCE
VIVTSDNPRTEDPQQILRDILAGIPGSPRAVEVDRRQAIRLAILAAQPGDTVVIAGKGHE
EEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHCEEEEEEEEECCCCEEEEECCCCC
DYQILGTEKIHFDDREEARAALALREKSRG
CEEEECCEEECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
METAGITLSKLLHQAGIQPQLAQGSLEGVQVWGLCADSRQVQPGDLFLGMPGSRVDGGSF
CCCCCHHHHHHHHHHCCCCCHHCCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCHHH
VAQALAAGAVAALISQEAWESVSGEAGWGSQPVLVLPRASLNRAVAQVAAAFYGFPARQL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEE
TLVGVTGTNGKTTTTHLIEHLVQAAGRSAALLGTLYNRWPGYSEVASHTTLFPPELQRSL
EEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHH
REAQRAGAEVAVMEVSSHALAQDRVWGCSFAAAVWTNLTQDHLDFHGSMENYWQAKATLF
HHHHHCCCEEEEEECCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHH
RQEYLQGRAVINADDAGGRRLLQAIRPDLQPWAYSLQPLQGIPGLWPSNVQLHPSGLQAT
HHHHHCCCEEEECCCCCHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCEECCCCCEEE
LHTPSGAFAVAAPLVGSFNLANLLAAVGVALHLGIPVEIIQSALPHFPGVPGRMERVTLP
EECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCEEEECC
GQDIAVIVDYAHTPDGLENLLKALRSQVQGQLICVFGCGGDRDRGKRPQMGRIAATWADQ
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCE
VIVTSDNPRTEDPQQILRDILAGIPGSPRAVEVDRRQAIRLAILAAQPGDTVVIAGKGHE
EEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHCEEEEEEEEECCCCEEEEECCCCC
DYQILGTEKIHFDDREEARAALALREKSRG
CEEEECCEEECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]