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The map label for this gene is gmd [H]

Identifier: 86608573

GI number: 86608573

Start: 1129404

End: 1130540

Strand: Reverse

Name: gmd [H]

Synonym: CYB_1095

Alternate gene names: 86608573

Gene position: 1130540-1129404 (Counterclockwise)

Preceding gene: 86608574

Following gene: 86608572

Centisome position: 37.11

GC content: 60.16

Gene sequence:

>1137_bases
ATGACCCAACGTGCCCTAATTACCGGCATCACCGGACAGGATGGCTCCTATCTGGCCGAGCTGTTGTTGAAGAAAAACTA
CGAAGTTCACGGCATCATCCGCCGCGCCAGCACCTTCAACACCGACCGCATCGACCACATCTACGTGGATGCCCATGACC
CCCAGGCTAGGCTATTCCTTCACTACGGGGATCTCACGGATGGCACCACCCTGCGCAGGATCCTGGAACAGGTGCAGCCG
ACGGAAGTGTACAACCTGGGGGCCCAATCCCACGTGCGGGTGAGCTTTGATGCGCCGGAATACACTGTTGATGCAGTGGC
CATGGGTACCCTGCGTCTCCTAGAGGCCATCCGCGACTACCAGCAGCGCACCGGGATCCGGGTGAAGTTTTACCAGGCAG
GCTCCTCGGAGATGTTCGGCCTGGTGCAGGAGATCCCGCAAAAGGAAACCACCCCCTTCTACCCGCGCAGCCCCTATGCC
TGTGCCAAGGTGTACGGCTACTGGCAGACGGTCAACTACCGCGAAGCCTACAACCTCTTCGCCTGCAACGGTATCTTGTT
CAACCACGAAAGCCCACGGCGAGGGGAAACCTTTGTAACCCGCAAGATCACCCGCGCCGTCAGCCGCATTGTGGCCGGCA
AACAGGACAAGCTCTACCTGGGCAACCTGGATGCCAAGCGGGACTGGGGCTACGCCAAAGACTACGTCGAGGGGATGTGG
ATGATGCTCCAACAACCCCAGCCGGATGACTATGTGCTGGCTACCGGGGAAACCCACACTGTGCGCGAGTTTGTGGAACG
GGCCTTCGCGCTGGTGGGCCTGCCGATTACCTGGAAAGGCTCCGGCCAAGCCGAACAGGGGCTGTACAAAGACCAAGTGT
TGATCGAGATCGATCCCCGCTACTACCGCCCAACCGAGGTGGATCTGCTGTTGGGGGATGCTTCAAAAGCCAAGCGAGTC
CTAGGATGGGAGCCCAAGGTGAGCTTCCCGCAGTTGGTGGAGCTGATGGTGCTGGCCGACTTGGAAGCCATCGGCCTGGA
TCCGGATCCCATCTTGGGGGAACGGCGCACCCTCAACGGCCTGCTCAGCGAAGATCGCGCTTTCATCCGCACCCAGCTGG
GGGTTCACTACGACTGA

Upstream 100 bases:

>100_bases
AGCTTCAAGGTCTCCGCTTTGAGGTTTGAGTTTTGCCATTGGACTTGAGCCTCAGGCCATTTTGATTTTTGGTGTTTTCC
TAGTCTTTGCCCCCAGCTCT

Downstream 100 bases:

>100_bases
CAGGGTGAATACAACGGTGGAGACGGGATCCCAAAAGACAGCCTTGACCCCTTGTCCAAAGCAGGAACTAAACGCTTAAT
ATAGTAGATCGCCGATTCTT

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTQRALITGITGQDGSYLAELLLKKNYEVHGIIRRASTFNTDRIDHIYVDAHDPQARLFLHYGDLTDGTTLRRILEQVQP
TEVYNLGAQSHVRVSFDAPEYTVDAVAMGTLRLLEAIRDYQQRTGIRVKFYQAGSSEMFGLVQEIPQKETTPFYPRSPYA
CAKVYGYWQTVNYREAYNLFACNGILFNHESPRRGETFVTRKITRAVSRIVAGKQDKLYLGNLDAKRDWGYAKDYVEGMW
MMLQQPQPDDYVLATGETHTVREFVERAFALVGLPITWKGSGQAEQGLYKDQVLIEIDPRYYRPTEVDLLLGDASKAKRV
LGWEPKVSFPQLVELMVLADLEAIGLDPDPILGERRTLNGLLSEDRAFIRTQLGVHYD

Sequences:

>Translated_378_residues
MTQRALITGITGQDGSYLAELLLKKNYEVHGIIRRASTFNTDRIDHIYVDAHDPQARLFLHYGDLTDGTTLRRILEQVQP
TEVYNLGAQSHVRVSFDAPEYTVDAVAMGTLRLLEAIRDYQQRTGIRVKFYQAGSSEMFGLVQEIPQKETTPFYPRSPYA
CAKVYGYWQTVNYREAYNLFACNGILFNHESPRRGETFVTRKITRAVSRIVAGKQDKLYLGNLDAKRDWGYAKDYVEGMW
MMLQQPQPDDYVLATGETHTVREFVERAFALVGLPITWKGSGQAEQGLYKDQVLIEIDPRYYRPTEVDLLLGDASKAKRV
LGWEPKVSFPQLVELMVLADLEAIGLDPDPILGERRTLNGLLSEDRAFIRTQLGVHYD
>Mature_377_residues
TQRALITGITGQDGSYLAELLLKKNYEVHGIIRRASTFNTDRIDHIYVDAHDPQARLFLHYGDLTDGTTLRRILEQVQPT
EVYNLGAQSHVRVSFDAPEYTVDAVAMGTLRLLEAIRDYQQRTGIRVKFYQAGSSEMFGLVQEIPQKETTPFYPRSPYAC
AKVYGYWQTVNYREAYNLFACNGILFNHESPRRGETFVTRKITRAVSRIVAGKQDKLYLGNLDAKRDWGYAKDYVEGMWM
MLQQPQPDDYVLATGETHTVREFVERAFALVGLPITWKGSGQAEQGLYKDQVLIEIDPRYYRPTEVDLLLGDASKAKRVL
GWEPKVSFPQLVELMVLADLEAIGLDPDPILGERRTLNGLLSEDRAFIRTQLGVHYD

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=353, Percent_Identity=60.9065155807365, Blast_Score=448, Evalue=1e-126,
Organism=Escherichia coli, GI1788366, Length=357, Percent_Identity=61.0644257703081, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1788353, Length=348, Percent_Identity=25, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994969, Length=336, Percent_Identity=25.5952380952381, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17539424, Length=347, Percent_Identity=60.5187319884726, Blast_Score=449, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI133901790, Length=347, Percent_Identity=60.5187319884726, Blast_Score=449, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17539422, Length=347, Percent_Identity=60.5187319884726, Blast_Score=448, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI133901788, Length=347, Percent_Identity=60.5187319884726, Blast_Score=448, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI133901786, Length=347, Percent_Identity=60.5187319884726, Blast_Score=448, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17507723, Length=347, Percent_Identity=60.2305475504323, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24158427, Length=348, Percent_Identity=63.5057471264368, Blast_Score=461, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 43129; Mature: 42997

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQRALITGITGQDGSYLAELLLKKNYEVHGIIRRASTFNTDRIDHIYVDAHDPQARLFL
CCCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCEEEEEECCCCCEEEEE
HYGDLTDGTTLRRILEQVQPTEVYNLGAQSHVRVSFDAPEYTVDAVAMGTLRLLEAIRDY
EECCCCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHH
QQRTGIRVKFYQAGSSEMFGLVQEIPQKETTPFYPRSPYACAKVYGYWQTVNYREAYNLF
HHHCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHEECCHHHHCCEE
ACNGILFNHESPRRGETFVTRKITRAVSRIVAGKQDKLYLGNLDAKRDWGYAKDYVEGMW
EECEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
MMLQQPQPDDYVLATGETHTVREFVERAFALVGLPITWKGSGQAEQGLYKDQVLIEIDPR
HHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCHHCCCCCCEEEEEECCC
YYRPTEVDLLLGDASKAKRVLGWEPKVSFPQLVELMVLADLEAIGLDPDPILGERRTLNG
CCCCCEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LLSEDRAFIRTQLGVHYD
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TQRALITGITGQDGSYLAELLLKKNYEVHGIIRRASTFNTDRIDHIYVDAHDPQARLFL
CCCEEEEEECCCCHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCEEEEEECCCCCEEEEE
HYGDLTDGTTLRRILEQVQPTEVYNLGAQSHVRVSFDAPEYTVDAVAMGTLRLLEAIRDY
EECCCCCCHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHH
QQRTGIRVKFYQAGSSEMFGLVQEIPQKETTPFYPRSPYACAKVYGYWQTVNYREAYNLF
HHHCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHEECCHHHHCCEE
ACNGILFNHESPRRGETFVTRKITRAVSRIVAGKQDKLYLGNLDAKRDWGYAKDYVEGMW
EECEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHH
MMLQQPQPDDYVLATGETHTVREFVERAFALVGLPITWKGSGQAEQGLYKDQVLIEIDPR
HHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCHHCCCCCCEEEEEECCC
YYRPTEVDLLLGDASKAKRVLGWEPKVSFPQLVELMVLADLEAIGLDPDPILGERRTLNG
CCCCCEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LLSEDRAFIRTQLGVHYD
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]