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The map label for this gene is spoT [H]

Identifier: 86608535

GI number: 86608535

Start: 1095279

End: 1095896

Strand: Direct

Name: spoT [H]

Synonym: CYB_1056

Alternate gene names: 86608535

Gene position: 1095279-1095896 (Clockwise)

Preceding gene: 86608534

Following gene: 86608536

Centisome position: 35.95

GC content: 58.41

Gene sequence:

>618_bases
ATGTCAACGCAACTCACACAGCGGTTTGTGCAGGCAATGCACTATGCCCATCGCTGGCATGCTTCTCAGACCCGCAAGGG
AACCGGGATCCCTTACCTCAGCCATCTGTTGGCCGTAGCAGCCATCGCCCTGGAGCATGGGGCGGATGAAGATGAAGCGA
TTGCCGCTCTGCTCCACGACAGCCTAGAGGACGGCCCCCACAACACAGGCGAGCCCCGGGCCCAGATTGAGCAACAGATC
CGGGAGCACTTTGGAGAACGGGTGCTGCAGATCATCCTGGGATGTACGGATACCGAAAGCGATGGCTCCCGAGGCGAGCC
CAAAGAAAACTGGAAAGAGCGCAAAACCGCCTACCTGAAGCACCTGGAAACGGCAAATGCCTCGGTTTTGTTGGTGGCCG
CCTCTGACAAGCTGCACAACGCCCGCTGCATCTTGCGGGATTTTCGCCAACTGGGGGATGAACTCTGGCAGCGGTTTTCC
GCCGGAAAAGAGGGATCCCTGTGGTATTATCGATCGTTGGCAGAGATTCTGAGGCGACGGCTGCCCGGCAATCTATCGGC
GGAGCTTCAAGAGCTGGTGGCTGAGTTGCAATGCCAAGCTGGAGCGGTAATGCCTTGA

Upstream 100 bases:

>100_bases
AGCACGAAGCCGCCCTTGCCGCGTCAGCGGGCTGTAGGCCTTAGGGCAGGGCTTGCAACCCAGTTTTCTTGGTCAACTTA
ACCCCAATTGGAGAGTCCAG

Downstream 100 bases:

>100_bases
CAGAGACAAGCAGGGCTGAAGAAAATGCTGAGGGCAGCCCTATTCATCACACTTTTTGGGTCACGCACCTATGGTTCTCG
CTGTTGGCACCCCTGCTCCT

Product: HD domain-containing protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MSTQLTQRFVQAMHYAHRWHASQTRKGTGIPYLSHLLAVAAIALEHGADEDEAIAALLHDSLEDGPHNTGEPRAQIEQQI
REHFGERVLQIILGCTDTESDGSRGEPKENWKERKTAYLKHLETANASVLLVAASDKLHNARCILRDFRQLGDELWQRFS
AGKEGSLWYYRSLAEILRRRLPGNLSAELQELVAELQCQAGAVMP

Sequences:

>Translated_205_residues
MSTQLTQRFVQAMHYAHRWHASQTRKGTGIPYLSHLLAVAAIALEHGADEDEAIAALLHDSLEDGPHNTGEPRAQIEQQI
REHFGERVLQIILGCTDTESDGSRGEPKENWKERKTAYLKHLETANASVLLVAASDKLHNARCILRDFRQLGDELWQRFS
AGKEGSLWYYRSLAEILRRRLPGNLSAELQELVAELQCQAGAVMP
>Mature_204_residues
STQLTQRFVQAMHYAHRWHASQTRKGTGIPYLSHLLAVAAIALEHGADEDEAIAALLHDSLEDGPHNTGEPRAQIEQQIR
EHFGERVLQIILGCTDTESDGSRGEPKENWKERKTAYLKHLETANASVLLVAASDKLHNARCILRDFRQLGDELWQRFSA
GKEGSLWYYRSLAEILRRRLPGNLSAELQELVAELQCQAGAVMP

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 23060; Mature: 22929

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQLTQRFVQAMHYAHRWHASQTRKGTGIPYLSHLLAVAAIALEHGADEDEAIAALLHD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SLEDGPHNTGEPRAQIEQQIREHFGERVLQIILGCTDTESDGSRGEPKENWKERKTAYLK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
HLETANASVLLVAASDKLHNARCILRDFRQLGDELWQRFSAGKEGSLWYYRSLAEILRRR
HHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LPGNLSAELQELVAELQCQAGAVMP
CCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
STQLTQRFVQAMHYAHRWHASQTRKGTGIPYLSHLLAVAAIALEHGADEDEAIAALLHD
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SLEDGPHNTGEPRAQIEQQIREHFGERVLQIILGCTDTESDGSRGEPKENWKERKTAYLK
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
HLETANASVLLVAASDKLHNARCILRDFRQLGDELWQRFSAGKEGSLWYYRSLAEILRRR
HHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LPGNLSAELQELVAELQCQAGAVMP
CCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]