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The map label for this gene is livK [H]

Identifier: 86608416

GI number: 86608416

Start: 974140

End: 975555

Strand: Direct

Name: livK [H]

Synonym: CYB_0936

Alternate gene names: 86608416

Gene position: 974140-975555 (Clockwise)

Preceding gene: 86608415

Following gene: 86608417

Centisome position: 31.97

GC content: 63.06

Gene sequence:

>1416_bases
ATGGTTGCCAAGTCTCCACCACCCATTGTCTACATTGCCTTGGGGCTATTGCTACTCTGGGGCGGTCATCGCGCTTGGCA
GGCGGGCTATGTTCGCTGGCCGGAAAGCATTTCTCTACCGCCAGTATCGCTGCCAGGGTTGGGGGGTGGAGCACAAGATC
TGCTCCTGACCAGCGCCGAGCGCACCTTGTTTGCCGATAGCAGTCCCGCTGCCGTTGCCGCTGCAGAGCTGTTGCGCCAA
GGTAACCTCAGCGCAGCCATCCCGGTGTTGGAGATGGGGCTGCAAAGCAACCGCAACGACCCGGAAGCTCGCATTTTCTT
GAACAATGCCCGTGCCCAGCAGCAGGGCAGTCCCTTGAGCCTGGCGGTGGTGGTGCCCATCTCCAGCAACCCCAACCGCG
CCAAAGAGACCCTGCGCGGGGTGGCGCAAGCTCAGGAGGAGCTTTTCCAACAGGGGGGAATCGGAGGACGGCTGCTGCGG
GTAGTGATTGCCGACGATGGCGACAACCCTGAGCGAGCAACGGCGGTGGCCAAGGCTCTGGTGAGCCAGCCCGAGATCTT
GGGGGTGATTGGCCATAACACCAGCGACGCCACCCTGGCTGCAGCCGAGGTTTATCAGCCTGCCGGCTTGGCCATGATCT
CCTCCACCTCCACCAGCACCCGCATCAGCATGCTGGGGGATGCCATTTTTCGCACCGTGCCCTCGGATCAAGTGGCCGGC
ACGCAACTGGCCCGTTATGCTCTCAGCACGTTGCGGCTGCAAAGGGTGGCCATCTTCTACAACCCCAACAGCGCTTACAG
TACCAGCCTGCGGGACGCCTTTGCCTCCACCCTGACCAGCGAAGGGGGCATCGTGCTGGCGGAGTTCGACTTATCGGCGT
CGGACTTCAACCCCCAGGCCAGCCTGCAAAGGGCCGTCGGCCAAGGAGCCCAGGTAGCTTTTTTAGCTCCCACGGCTTCC
GTAGCCGAAAAAGCGCTGGCCGTAGCCCGCGAGAACGCTGTAGCTGGTAAGCCCCTTGCTCTTCTGGGCGGCGATGTGCT
CTTCAGCCTCACCACATTGCAAGACGGGGGAGCAGCGGTAGAAGGCTTGGTGGTGGCGGTACCCTGGCATCCCCAGGTGC
CGCGCAACCGCGAGTTTGCCCAGAAGGCAGCCCAGCTCTGGGGAGGGCAGGTGAGCTGGCGCACCGCTCTGGCTTATGAT
GCCACCCGGGTTTTCTTGCAAGCTTTCAGCCAAGGAGCCACCAGCCGCAACGCTGTGCTGGCCCGTTTGCAGGATCCCAA
CTTCCTCCTCCAGGACGGCGCTTCCGGAGAAGTGCGCTTCTTGCCCTCCGGCGACCGCAATGCTCCCCATGTGCTGGTGC
GGGTTGAGCCCGGCAACCGCTCCGGTACCGGCTACGATTTTGTGCCTCTCCCCTAG

Upstream 100 bases:

>100_bases
TTAGAGATCCTTGGAAAGATGACTTTTGAGGATTCCTGAGGATACAAGCAGTGTCTTTGCTGGCCAAAAGCTAGCGTTTT
CTCTCTCTATAGTGTGATAT

Downstream 100 bases:

>100_bases
GGATCCCGGCAGACGCTACAGTGGGGGATGCTCTCAATCTCCTCAGAATCGAATGGATCCCATGCCCAGCCGGATCACAG
CCTGTTTCCAGGCGCTGCGC

Product: hydrophobic amino acid uptake ABC transporter periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: L-BP; LS-BP [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MVAKSPPPIVYIALGLLLLWGGHRAWQAGYVRWPESISLPPVSLPGLGGGAQDLLLTSAERTLFADSSPAAVAAAELLRQ
GNLSAAIPVLEMGLQSNRNDPEARIFLNNARAQQQGSPLSLAVVVPISSNPNRAKETLRGVAQAQEELFQQGGIGGRLLR
VVIADDGDNPERATAVAKALVSQPEILGVIGHNTSDATLAAAEVYQPAGLAMISSTSTSTRISMLGDAIFRTVPSDQVAG
TQLARYALSTLRLQRVAIFYNPNSAYSTSLRDAFASTLTSEGGIVLAEFDLSASDFNPQASLQRAVGQGAQVAFLAPTAS
VAEKALAVARENAVAGKPLALLGGDVLFSLTTLQDGGAAVEGLVVAVPWHPQVPRNREFAQKAAQLWGGQVSWRTALAYD
ATRVFLQAFSQGATSRNAVLARLQDPNFLLQDGASGEVRFLPSGDRNAPHVLVRVEPGNRSGTGYDFVPLP

Sequences:

>Translated_471_residues
MVAKSPPPIVYIALGLLLLWGGHRAWQAGYVRWPESISLPPVSLPGLGGGAQDLLLTSAERTLFADSSPAAVAAAELLRQ
GNLSAAIPVLEMGLQSNRNDPEARIFLNNARAQQQGSPLSLAVVVPISSNPNRAKETLRGVAQAQEELFQQGGIGGRLLR
VVIADDGDNPERATAVAKALVSQPEILGVIGHNTSDATLAAAEVYQPAGLAMISSTSTSTRISMLGDAIFRTVPSDQVAG
TQLARYALSTLRLQRVAIFYNPNSAYSTSLRDAFASTLTSEGGIVLAEFDLSASDFNPQASLQRAVGQGAQVAFLAPTAS
VAEKALAVARENAVAGKPLALLGGDVLFSLTTLQDGGAAVEGLVVAVPWHPQVPRNREFAQKAAQLWGGQVSWRTALAYD
ATRVFLQAFSQGATSRNAVLARLQDPNFLLQDGASGEVRFLPSGDRNAPHVLVRVEPGNRSGTGYDFVPLP
>Mature_471_residues
MVAKSPPPIVYIALGLLLLWGGHRAWQAGYVRWPESISLPPVSLPGLGGGAQDLLLTSAERTLFADSSPAAVAAAELLRQ
GNLSAAIPVLEMGLQSNRNDPEARIFLNNARAQQQGSPLSLAVVVPISSNPNRAKETLRGVAQAQEELFQQGGIGGRLLR
VVIADDGDNPERATAVAKALVSQPEILGVIGHNTSDATLAAAEVYQPAGLAMISSTSTSTRISMLGDAIFRTVPSDQVAG
TQLARYALSTLRLQRVAIFYNPNSAYSTSLRDAFASTLTSEGGIVLAEFDLSASDFNPQASLQRAVGQGAQVAFLAPTAS
VAEKALAVARENAVAGKPLALLGGDVLFSLTTLQDGGAAVEGLVVAVPWHPQVPRNREFAQKAAQLWGGQVSWRTALAYD
ATRVFLQAFSQGATSRNAVLARLQDPNFLLQDGASGEVRFLPSGDRNAPHVLVRVEPGNRSGTGYDFVPLP

Specific function: This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=245, Percent_Identity=27.7551020408163, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789867, Length=163, Percent_Identity=32.5153374233129, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 49509; Mature: 49509

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAKSPPPIVYIALGLLLLWGGHRAWQAGYVRWPESISLPPVSLPGLGGGAQDLLLTSAE
CCCCCCCCCHHHHHHHHHHHCCCCHHCCCCEECCCCCCCCCCCCCCCCCCHHHHHEECCC
RTLFADSSPAAVAAAELLRQGNLSAAIPVLEMGLQSNRNDPEARIFLNNARAQQQGSPLS
CEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCCEE
LAVVVPISSNPNRAKETLRGVAQAQEELFQQGGIGGRLLRVVIADDGDNPERATAVAKAL
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHH
VSQPEILGVIGHNTSDATLAAAEVYQPAGLAMISSTSTSTRISMLGDAIFRTVPSDQVAG
HCCCCEEEEECCCCCCCCEEHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHH
TQLARYALSTLRLQRVAIFYNPNSAYSTSLRDAFASTLTSEGGIVLAEFDLSASDFNPQA
HHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHH
SLQRAVGQGAQVAFLAPTASVAEKALAVARENAVAGKPLALLGGDVLFSLTTLQDGGAAV
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHEECCCEEEEEEEECCCCCEE
EGLVVAVPWHPQVPRNREFAQKAAQLWGGQVSWRTALAYDATRVFLQAFSQGATSRNAVL
CCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCEEE
ARLQDPNFLLQDGASGEVRFLPSGDRNAPHVLVRVEPGNRSGTGYDFVPLP
EEECCCCEEEECCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCEEECCCC
>Mature Secondary Structure
MVAKSPPPIVYIALGLLLLWGGHRAWQAGYVRWPESISLPPVSLPGLGGGAQDLLLTSAE
CCCCCCCCCHHHHHHHHHHHCCCCHHCCCCEECCCCCCCCCCCCCCCCCCHHHHHEECCC
RTLFADSSPAAVAAAELLRQGNLSAAIPVLEMGLQSNRNDPEARIFLNNARAQQQGSPLS
CEEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCCEE
LAVVVPISSNPNRAKETLRGVAQAQEELFQQGGIGGRLLRVVIADDGDNPERATAVAKAL
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHH
VSQPEILGVIGHNTSDATLAAAEVYQPAGLAMISSTSTSTRISMLGDAIFRTVPSDQVAG
HCCCCEEEEECCCCCCCCEEHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHH
TQLARYALSTLRLQRVAIFYNPNSAYSTSLRDAFASTLTSEGGIVLAEFDLSASDFNPQA
HHHHHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHH
SLQRAVGQGAQVAFLAPTASVAEKALAVARENAVAGKPLALLGGDVLFSLTTLQDGGAAV
HHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHEECCCEEEEEEEECCCCCEE
EGLVVAVPWHPQVPRNREFAQKAAQLWGGQVSWRTALAYDATRVFLQAFSQGATSRNAVL
CCEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCEEE
ARLQDPNFLLQDGASGEVRFLPSGDRNAPHVLVRVEPGNRSGTGYDFVPLP
EEECCCCEEEECCCCCCEEEECCCCCCCCEEEEEECCCCCCCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3891753; 4077929; 2195019; 8041620; 9278503; 6990419; 9298646; 2649683 [H]