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The map label for this gene is hisH

Identifier: 86608369

GI number: 86608369

Start: 921848

End: 922504

Strand: Reverse

Name: hisH

Synonym: CYB_0889

Alternate gene names: 86608369

Gene position: 922504-921848 (Counterclockwise)

Preceding gene: 86608371

Following gene: 86608368

Centisome position: 30.28

GC content: 60.12

Gene sequence:

>657_bases
ATGGGAGCTGTGCGCCTGGCGGTAATCGACTATGAGGCGGGAAATCTGCATTCGGCTTGCAAAGGGCTGGAGCGGGCCGG
AGCTGAGGTGCAGCTAGTAACAGAGCCAACTGGACTAGCGGCTTTTGACGGCATTGTCCTGCCCGGCGATGGGGCCTTTG
ACCCAGCCATGCAGCAGCTGCGGGCGCGGGGCTTTGGGAAAGCGATCCGGGAAGCTGTGGAGCGGCAACAGCCTTTTTTA
GGCATTTGCCTTGGGCTGCAAGTTTTGTTCGAAAGCAGCGAAGAGGGAACAGAGCCAGGGCTGAGCATTGTGCCTGGACG
GGTGCAGCGCTTTCGGCCAGAGGCGGGCCTGCGCATCCCTCACATGGGCTGGAATCAACTGCAACTCACCCAAGCCCACT
CTCCCCTCTGGGCAGGGATCCCCGATCAAACCTGGGCCTATTTTGTCCATTCCTATCACGGTGTGCCCAGCGATCCAGCC
TGGGTGGCTGCCACGACCCAACACGGCAGCCAAACCTGTGTAGCCGCCATTGCCCGCGGCGCTTTGTTTGCTACCCAGTT
TCACCCAGAAAAGTCCGGCCCCTACGGCTTGAAGATGCTGCGCAACTTTGTCGAGTTTGTTGCCCGTTGTTCCCCCTTAT
CTGCTCCCAGAGCCTAG

Upstream 100 bases:

>100_bases
GTGGGATCGTGCGAAGCAATTGCCACAGCCATGAGTCAGCTCAAACGGGTTAGAACCACTATACTGATAGACTCCGAAAC
TCCTCACGAGGAGGGATCCC

Downstream 100 bases:

>100_bases
TGCCCCATGAACTACTGGCTGATGAAATCTGAGCCCAGCATCTATAGCATCTGGGATTTGCAGCGGGAGGGCCAAACCCT
TTGGGATGGCGTGCGCAACT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MGAVRLAVIDYEAGNLHSACKGLERAGAEVQLVTEPTGLAAFDGIVLPGDGAFDPAMQQLRARGFGKAIREAVERQQPFL
GICLGLQVLFESSEEGTEPGLSIVPGRVQRFRPEAGLRIPHMGWNQLQLTQAHSPLWAGIPDQTWAYFVHSYHGVPSDPA
WVAATTQHGSQTCVAAIARGALFATQFHPEKSGPYGLKMLRNFVEFVARCSPLSAPRA

Sequences:

>Translated_218_residues
MGAVRLAVIDYEAGNLHSACKGLERAGAEVQLVTEPTGLAAFDGIVLPGDGAFDPAMQQLRARGFGKAIREAVERQQPFL
GICLGLQVLFESSEEGTEPGLSIVPGRVQRFRPEAGLRIPHMGWNQLQLTQAHSPLWAGIPDQTWAYFVHSYHGVPSDPA
WVAATTQHGSQTCVAAIARGALFATQFHPEKSGPYGLKMLRNFVEFVARCSPLSAPRA
>Mature_217_residues
GAVRLAVIDYEAGNLHSACKGLERAGAEVQLVTEPTGLAAFDGIVLPGDGAFDPAMQQLRARGFGKAIREAVERQQPFLG
ICLGLQVLFESSEEGTEPGLSIVPGRVQRFRPEAGLRIPHMGWNQLQLTQAHSPLWAGIPDQTWAYFVHSYHGVPSDPAW
VAATTQHGSQTCVAAIARGALFATQFHPEKSGPYGLKMLRNFVEFVARCSPLSAPRA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=204, Percent_Identity=36.7647058823529, Blast_Score=120, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=36.3636363636364, Blast_Score=134, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_SYNJB (Q2JN09)

Other databases:

- EMBL:   CP000240
- RefSeq:   YP_477131.1
- ProteinModelPortal:   Q2JN09
- SMR:   Q2JN09
- STRING:   Q2JN09
- GeneID:   3902726
- GenomeReviews:   CP000240_GR
- KEGG:   cyb:CYB_0889
- TIGR:   CYB_0889
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- PhylomeDB:   Q2JN09
- ProtClustDB:   PRK13141
- BioCyc:   SSP321332:CYB_0889-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23516; Mature: 23385

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 83-83 ACT_SITE 188-188 ACT_SITE 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAVRLAVIDYEAGNLHSACKGLERAGAEVQLVTEPTGLAAFDGIVLPGDGAFDPAMQQL
CCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHCCCEEECCCCCCCHHHHHH
RARGFGKAIREAVERQQPFLGICLGLQVLFESSEEGTEPGLSIVPGRVQRFRPEAGLRIP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHCCCCCCCCCC
HMGWNQLQLTQAHSPLWAGIPDQTWAYFVHSYHGVPSDPAWVAATTQHGSQTCVAAIARG
CCCCCCEEEECCCCCEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHH
ALFATQFHPEKSGPYGLKMLRNFVEFVARCSPLSAPRA
HHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GAVRLAVIDYEAGNLHSACKGLERAGAEVQLVTEPTGLAAFDGIVLPGDGAFDPAMQQL
CCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHCCCEEECCCCCCCHHHHHH
RARGFGKAIREAVERQQPFLGICLGLQVLFESSEEGTEPGLSIVPGRVQRFRPEAGLRIP
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCEECCCHHHHCCCCCCCCCC
HMGWNQLQLTQAHSPLWAGIPDQTWAYFVHSYHGVPSDPAWVAATTQHGSQTCVAAIARG
CCCCCCEEEECCCCCEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHH
ALFATQFHPEKSGPYGLKMLRNFVEFVARCSPLSAPRA
HHHEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA