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The map label for this gene is argF [H]

Identifier: 86608303

GI number: 86608303

Start: 850394

End: 851338

Strand: Reverse

Name: argF [H]

Synonym: CYB_0821

Alternate gene names: 86608303

Gene position: 851338-850394 (Counterclockwise)

Preceding gene: 86608304

Following gene: 86608300

Centisome position: 27.94

GC content: 62.86

Gene sequence:

>945_bases
ATGGCGGGGTCGAAGATCGCGGGATCCATAAACTTGGCCGGACGGGATCTGCTCAAGCTCAGCGACCTAACCCCGGCGGA
GCTGTCGGCCCTGCTAGCCCAGGCGGCTGAGTTAAAGCGGGATCCCTATCGGGTGGAGCTGCGGGGTCGGGTGTTGGGGC
TGTTGTTTCAGAAGGCTTCTACCCGCACCCGCGCCAGTTTTACGGCAGCCATGGCCCAATGCGGGGGCAGCACCCTCGAT
CTGGTGCCGACAGTGATGCAGGTGAGCCGCGGCGAGCCCCTGCAGGATACGGCCCGCGTCCTCTCCCGCTACCTGGATGT
GATCGCCATTCGCACCTACGACCAGAAGGAGCTGGAAACCTTTGCCGAGTATGCCGATGTTCCGGTGATCAACGCCCTCA
CCGATCAGTTTCACCCCTGTCAGGTGTTAGCCGATCTGCTCACCATTCAGGAGAACTTTGGCCAACTGGCGGGCCTGACC
CTGGCCTATTGCGGCGATGGCAATAACGTTGCCCACTCCCTGCTGCTGGGCTGTGCCATGAGCGGGATCCACATCCGCGT
CGCCACCCCCCCCGGTTACGAGCCGGATGCGGCAGTGGTGGAGCAAGCCCGCACTCTGGCCCAGGGATCCCAGGTGTTGG
TGCTGCAGGATCCCCAGGAAGCCGTCTCTGGATCCCAGGTGGTCTATACCGACGTGTGGGCCAGCATGGGTCAAGAAGCT
GAGGCCGAGGCCCGCAAGGCCACCTTTCTGCCCTACCAGGTCAATGCCGAGCTGCTGCAACGGGCGGATCCGCAAGCCAT
TGTGCTGCACTGCCTGCCGGCCCACCGGGAAGAGGAAATCACCACTGAAGTACTGGAGGGATCCCAGTCCCGCGTGTGGG
ATCAGGCGGAAAATCGCCTACATGTGCAAAAGGCACTCCTGATTCAGGTGCTGGGCGCAGGATGA

Upstream 100 bases:

>100_bases
TAAGACTATTAAGACTCTTCTCATTCGAGTTATTCGAGGAGTGTTGACCAGCGCCGGGCTGGGGATGCTTTTATTGTTTC
TGTAGGTGGCGCGTAGCACA

Downstream 100 bases:

>100_bases
CCCCTCACCCCCAGGCTAGAGAGCGGGTTAGCAAATGAGCTTACCGGAAAACTGGGTCTATGGTCATTTCGATTTAAACC
AGGACACTGCACCCACACCC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MAGSKIAGSINLAGRDLLKLSDLTPAELSALLAQAAELKRDPYRVELRGRVLGLLFQKASTRTRASFTAAMAQCGGSTLD
LVPTVMQVSRGEPLQDTARVLSRYLDVIAIRTYDQKELETFAEYADVPVINALTDQFHPCQVLADLLTIQENFGQLAGLT
LAYCGDGNNVAHSLLLGCAMSGIHIRVATPPGYEPDAAVVEQARTLAQGSQVLVLQDPQEAVSGSQVVYTDVWASMGQEA
EAEARKATFLPYQVNAELLQRADPQAIVLHCLPAHREEEITTEVLEGSQSRVWDQAENRLHVQKALLIQVLGAG

Sequences:

>Translated_314_residues
MAGSKIAGSINLAGRDLLKLSDLTPAELSALLAQAAELKRDPYRVELRGRVLGLLFQKASTRTRASFTAAMAQCGGSTLD
LVPTVMQVSRGEPLQDTARVLSRYLDVIAIRTYDQKELETFAEYADVPVINALTDQFHPCQVLADLLTIQENFGQLAGLT
LAYCGDGNNVAHSLLLGCAMSGIHIRVATPPGYEPDAAVVEQARTLAQGSQVLVLQDPQEAVSGSQVVYTDVWASMGQEA
EAEARKATFLPYQVNAELLQRADPQAIVLHCLPAHREEEITTEVLEGSQSRVWDQAENRLHVQKALLIQVLGAG
>Mature_313_residues
AGSKIAGSINLAGRDLLKLSDLTPAELSALLAQAAELKRDPYRVELRGRVLGLLFQKASTRTRASFTAAMAQCGGSTLDL
VPTVMQVSRGEPLQDTARVLSRYLDVIAIRTYDQKELETFAEYADVPVINALTDQFHPCQVLADLLTIQENFGQLAGLTL
AYCGDGNNVAHSLLLGCAMSGIHIRVATPPGYEPDAAVVEQARTLAQGSQVLVLQDPQEAVSGSQVVYTDVWASMGQEAE
AEARKATFLPYQVNAELLQRADPQAIVLHCLPAHREEEITTEVLEGSQSRVWDQAENRLHVQKALLIQVLGAG

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=316, Percent_Identity=41.1392405063291, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=30.1282051282051, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1790703, Length=326, Percent_Identity=41.4110429447853, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1786469, Length=331, Percent_Identity=38.9728096676737, Blast_Score=224, Evalue=8e-60,
Organism=Escherichia coli, GI2367364, Length=316, Percent_Identity=31.9620253164557, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI193204318, Length=337, Percent_Identity=29.3768545994065, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322373, Length=308, Percent_Identity=33.7662337662338, Blast_Score=193, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322331, Length=308, Percent_Identity=31.1688311688312, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24642586, Length=316, Percent_Identity=30.0632911392405, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 33959; Mature: 33828

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGSKIAGSINLAGRDLLKLSDLTPAELSALLAQAAELKRDPYRVELRGRVLGLLFQKAS
CCCCCCCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHC
TRTRASFTAAMAQCGGSTLDLVPTVMQVSRGEPLQDTARVLSRYLDVIAIRTYDQKELET
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
FAEYADVPVINALTDQFHPCQVLADLLTIQENFGQLAGLTLAYCGDGNNVAHSLLLGCAM
HHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHH
SGIHIRVATPPGYEPDAAVVEQARTLAQGSQVLVLQDPQEAVSGSQVVYTDVWASMGQEA
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHCCCEEEEHHHHHHCCCHH
EAEARKATFLPYQVNAELLQRADPQAIVLHCLPAHREEEITTEVLEGSQSRVWDQAENRL
HHHHHHHCCCCEECCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
HVQKALLIQVLGAG
HHHHHHHHHHHCCC
>Mature Secondary Structure 
AGSKIAGSINLAGRDLLKLSDLTPAELSALLAQAAELKRDPYRVELRGRVLGLLFQKAS
CCCCCCCEEECCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHC
TRTRASFTAAMAQCGGSTLDLVPTVMQVSRGEPLQDTARVLSRYLDVIAIRTYDQKELET
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
FAEYADVPVINALTDQFHPCQVLADLLTIQENFGQLAGLTLAYCGDGNNVAHSLLLGCAM
HHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHH
SGIHIRVATPPGYEPDAAVVEQARTLAQGSQVLVLQDPQEAVSGSQVVYTDVWASMGQEA
CCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHCCCEEEEHHHHHHCCCHH
EAEARKATFLPYQVNAELLQRADPQAIVLHCLPAHREEEITTEVLEGSQSRVWDQAENRL
HHHHHHHCCCCEECCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHH
HVQKALLIQVLGAG
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA