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The map label for this gene is ygcM [C]

Identifier: 86608161

GI number: 86608161

Start: 687275

End: 688180

Strand: Direct

Name: ygcM [C]

Synonym: CYB_0675

Alternate gene names: 86608161

Gene position: 687275-688180 (Clockwise)

Preceding gene: 86608160

Following gene: 86608162

Centisome position: 22.56

GC content: 57.84

Gene sequence:

>906_bases
ATGTCCAGAATCTGTACAATCTACCGCCGTGCGCAATTTGCCGCCAGCCATCGGTACTGGTTGCCCGAACTCTCAGAAGC
CGAGAACTTAGCCCGCTTTGGCCCAAATGCCCGTTTTCCCGGCCATGGCCACAACTATGTGCTGGAGGTGGGCATGAGTG
GTGAGGTGGATGACTATGGGATGGTGCTCAACCTCTCGGAGGTGAAGCAGGTGATCCGGCAGCGGGTGATTCAAGATCTC
AACTTTGCTTATCTAAATCAAGCTTGGCCGGAGTTTGCCCAAACCTTGCCCACTACCGAGTTTATCGCTTACGTGATTTG
GCAACGCCTGGCCGATGCCTTGCCCCTAACCAGCGTCCGTCTGTACGAAGATCCTGAGCTTTGGGCCGAATACCGAGGAG
AAGCCATGCAAGCCTACTTGACTGTTGCCACCCATTTCAGCGCCGCCCACCGCTTGGCTCTGGATCACCTGAGTTTTGAA
GAAAACAGCGCCATCTACGGCCTCTGTGCCCGTCCCCACGGCCACGGCCACAACTACGGCCTGGAGATCACCGTCAAGGG
ATCCATCGACCCGCGCACGGGGATGATCGTGGACTTGGCGGCCCTGCAGCGGCTGATCGAGGAGCAGGTGGTGAAGCCGC
TGGATCACACCTTTTTGAACAAAGACATTCCCTACTTTGCCCAGGTGGTGCCTACTGCCGAAAACATCGCCCTCTACATC
CAGCGGCTGCTGACTCTGCCCCTGCGGGAGCTGGGGGTGCAGTTGCATCGGGTTCGCCTGCAGGAGAGTCCCAACAACAG
CAGCGAGGTCTACGGGGAGTTTCCGCTGGAAGAGCTGCTCAACTCCTCAGAAGGACAAGATGTGGCTTCTTCCGCTTCTG
GCAGCCGTATTGCCATGCAGGGCTGA

Upstream 100 bases:

>100_bases
GGAGGGTAGGCTGAGGGCCGCTGAGAATTTCCACCCTCTGGGCCGGGGAGTGTGTCAAGCTAAAGGGTGGATCCCCCGGC
AGATGCTCTCCTCTCCACTC

Downstream 100 bases:

>100_bases
TGCGGCCATCATGTTGGATCTGGTTAAGCTGAGCGGGCAAATTCCTGCGTTCACCCGGCACCTGCAACAGGAGTCGAAAG
CCAGCCAAGAACGCCTCCGC

Product: 6-pyruvoyl-tetrahydropterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl-Tetrahydropterin Synthase-Like Protein; 6-Pyruvoyl Tetrahydrobiopterin Synthase; 6-Pyruvoyl Tetrahydropterin Synthase Family; 6-Pyruvoyl Tetrahydropterin Synthase Family Protein; Tetrahydrobiopterin Biosynthesis-Like Protein; 6-Pyruvoyltetrahydrobiopterin Synthase; Queuosine Biosynthesis Protein QueD; 6-Pyruvoyltetrahydropterin Synthase Synthase

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSRICTIYRRAQFAASHRYWLPELSEAENLARFGPNARFPGHGHNYVLEVGMSGEVDDYGMVLNLSEVKQVIRQRVIQDL
NFAYLNQAWPEFAQTLPTTEFIAYVIWQRLADALPLTSVRLYEDPELWAEYRGEAMQAYLTVATHFSAAHRLALDHLSFE
ENSAIYGLCARPHGHGHNYGLEITVKGSIDPRTGMIVDLAALQRLIEEQVVKPLDHTFLNKDIPYFAQVVPTAENIALYI
QRLLTLPLRELGVQLHRVRLQESPNNSSEVYGEFPLEELLNSSEGQDVASSASGSRIAMQG

Sequences:

>Translated_301_residues
MSRICTIYRRAQFAASHRYWLPELSEAENLARFGPNARFPGHGHNYVLEVGMSGEVDDYGMVLNLSEVKQVIRQRVIQDL
NFAYLNQAWPEFAQTLPTTEFIAYVIWQRLADALPLTSVRLYEDPELWAEYRGEAMQAYLTVATHFSAAHRLALDHLSFE
ENSAIYGLCARPHGHGHNYGLEITVKGSIDPRTGMIVDLAALQRLIEEQVVKPLDHTFLNKDIPYFAQVVPTAENIALYI
QRLLTLPLRELGVQLHRVRLQESPNNSSEVYGEFPLEELLNSSEGQDVASSASGSRIAMQG
>Mature_300_residues
SRICTIYRRAQFAASHRYWLPELSEAENLARFGPNARFPGHGHNYVLEVGMSGEVDDYGMVLNLSEVKQVIRQRVIQDLN
FAYLNQAWPEFAQTLPTTEFIAYVIWQRLADALPLTSVRLYEDPELWAEYRGEAMQAYLTVATHFSAAHRLALDHLSFEE
NSAIYGLCARPHGHGHNYGLEITVKGSIDPRTGMIVDLAALQRLIEEQVVKPLDHTFLNKDIPYFAQVVPTAENIALYIQ
RLLTLPLRELGVQLHRVRLQESPNNSSEVYGEFPLEELLNSSEGQDVASSASGSRIAMQG

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506331, Length=147, Percent_Identity=45.578231292517, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532226, Length=121, Percent_Identity=39.6694214876033, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI115532224, Length=121, Percent_Identity=39.6694214876033, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19550074, Length=144, Percent_Identity=43.75, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24585378, Length=144, Percent_Identity=43.75, Blast_Score=120, Evalue=8e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 33999; Mature: 33868

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRICTIYRRAQFAASHRYWLPELSEAENLARFGPNARFPGHGHNYVLEVGMSGEVDDYG
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCC
MVLNLSEVKQVIRQRVIQDLNFAYLNQAWPEFAQTLPTTEFIAYVIWQRLADALPLTSVR
EEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEE
LYEDPELWAEYRGEAMQAYLTVATHFSAAHRLALDHLSFEENSAIYGLCARPHGHGHNYG
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCC
LEITVKGSIDPRTGMIVDLAALQRLIEEQVVKPLDHTFLNKDIPYFAQVVPTAENIALYI
EEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHH
QRLLTLPLRELGVQLHRVRLQESPNNSSEVYGEFPLEELLNSSEGQDVASSASGSRIAMQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHCCCCCCCCHHHCCCCCEEEEC
G
C
>Mature Secondary Structure 
SRICTIYRRAQFAASHRYWLPELSEAENLARFGPNARFPGHGHNYVLEVGMSGEVDDYG
CHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCC
MVLNLSEVKQVIRQRVIQDLNFAYLNQAWPEFAQTLPTTEFIAYVIWQRLADALPLTSVR
EEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEE
LYEDPELWAEYRGEAMQAYLTVATHFSAAHRLALDHLSFEENSAIYGLCARPHGHGHNYG
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCC
LEITVKGSIDPRTGMIVDLAALQRLIEEQVVKPLDHTFLNKDIPYFAQVVPTAENIALYI
EEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHH
QRLLTLPLRELGVQLHRVRLQESPNNSSEVYGEFPLEELLNSSEGQDVASSASGSRIAMQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCHHHHCCCCCCCCHHHCCCCCEEEEC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA