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The map label for this gene is nudF [H]

Identifier: 86608028

GI number: 86608028

Start: 551297

End: 551845

Strand: Direct

Name: nudF [H]

Synonym: CYB_0536

Alternate gene names: 86608028

Gene position: 551297-551845 (Clockwise)

Preceding gene: 86608023

Following gene: 86608029

Centisome position: 18.09

GC content: 53.55

Gene sequence:

>549_bases
ATGCAAAACCCATCTCAGGTGATTCGTGAGCGCCTGCGCTATCAAGGCCATAAATACACCTTCGTCAGCCAGCGTTTGCG
TTTTCCCAACGGCAAAGAGGGAGAGCGGGAGTACCTCATCCATCCCGGGGGGGTGGTGGCCGTGCCTGTAACTGCAGCAG
GCAAGTTCGTCTGCATTCGTCAATATCGCTTTGCGGTCGCTTCCTATCTTTACGAATTCCCGGCAGGCACAGTCGAGCCA
GGGGAGCATCCCGATGACACCATTCGCCGCGAGCTGGAAGAAGAAACGGGCCTGCGTGCCCATCGCTGGGATCCCCTTGG
CCAATTTTATTTGTGCCCCGGCTATTCCAGCGAGATTATGTATGCCTATTTGGCGCGGGAGCTGGAAGTGCTGGATTCCC
CTCCCGATAAAGATGAGGATGAAGATATTACCGTAGTGGAGTTTTCTGCTTCTGAGCTTACAGAAATGGCCCGTTTGGGA
ATGGATTTGGACAGCAAGTCCATTGCCTGCTTTTGGCGGGCCCAATTGTTTTTGGCTGCTGAAAAATAA

Upstream 100 bases:

>100_bases
ACGGGATCCCAAGGGCGTGTGGATCCCTCTGGATTGCCCGGTTGCAACAGCGCCGGAAACCATTAAGCTGAGAGCGGCTC
TACTCTCGGGATCCCTTGCC

Downstream 100 bases:

>100_bases
AGCTCTCCGTCGGGGGGCTGCCCCTTGCTCCTGTAGGATCCTTTCCAATTGGCGGGGCGTCTCTCCAAGCCGACTTTGGC
GGTTACAGGCGAATCTTGGG

Product: NUDIX family hydrolase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MQNPSQVIRERLRYQGHKYTFVSQRLRFPNGKEGEREYLIHPGGVVAVPVTAAGKFVCIRQYRFAVASYLYEFPAGTVEP
GEHPDDTIRRELEEETGLRAHRWDPLGQFYLCPGYSSEIMYAYLARELEVLDSPPDKDEDEDITVVEFSASELTEMARLG
MDLDSKSIACFWRAQLFLAAEK

Sequences:

>Translated_182_residues
MQNPSQVIRERLRYQGHKYTFVSQRLRFPNGKEGEREYLIHPGGVVAVPVTAAGKFVCIRQYRFAVASYLYEFPAGTVEP
GEHPDDTIRRELEEETGLRAHRWDPLGQFYLCPGYSSEIMYAYLARELEVLDSPPDKDEDEDITVVEFSASELTEMARLG
MDLDSKSIACFWRAQLFLAAEK
>Mature_182_residues
MQNPSQVIRERLRYQGHKYTFVSQRLRFPNGKEGEREYLIHPGGVVAVPVTAAGKFVCIRQYRFAVASYLYEFPAGTVEP
GEHPDDTIRRELEEETGLRAHRWDPLGQFYLCPGYSSEIMYAYLARELEVLDSPPDKDEDEDITVVEFSASELTEMARLG
MDLDSKSIACFWRAQLFLAAEK

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 20890; Mature: 20890

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNPSQVIRERLRYQGHKYTFVSQRLRFPNGKEGEREYLIHPGGVVAVPVTAAGKFVCIR
CCCHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCCEEHHH
QYRFAVASYLYEFPAGTVEPGEHPDDTIRRELEEETGLRAHRWDPLGQFYLCPGYSSEIM
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHH
YAYLARELEVLDSPPDKDEDEDITVVEFSASELTEMARLGMDLDSKSIACFWRAQLFLAA
HHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEE
EK
CC
>Mature Secondary Structure
MQNPSQVIRERLRYQGHKYTFVSQRLRFPNGKEGEREYLIHPGGVVAVPVTAAGKFVCIR
CCCHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCCEEHHH
QYRFAVASYLYEFPAGTVEPGEHPDDTIRRELEEETGLRAHRWDPLGQFYLCPGYSSEIM
HHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHH
YAYLARELEVLDSPPDKDEDEDITVVEFSASELTEMARLGMDLDSKSIACFWRAQLFLAA
HHHHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEE
EK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]