Click here to switch to the map view.

The map label for this gene is aspA [H]

Identifier: 86608019

GI number: 86608019

Start: 540591

End: 541976

Strand: Direct

Name: aspA [H]

Synonym: CYB_0526

Alternate gene names: 86608019

Gene position: 540591-541976 (Clockwise)

Preceding gene: 86608018

Following gene: 86608020

Centisome position: 17.74

GC content: 62.34

Gene sequence:

>1386_bases
GTGCGCAGCGAACGAGACTCAATGGGGGAGCGGCAACTACCCCAGTCGGTTTACTACGGCATCCAGACCGCCCGTGCCCT
GGAGAACTTCCCCATCAGCGGCCTCAAGCCCCTGCCAGAGTATGTGGAGGCCTGCGTGCTGATTAAAAAGGCGGCGGCGG
AGGTCAACGGCGAGCTGGGGTGCATTCCGGCAGAGGTGGCCAAGGCGATTGTCCAAGCGGCGGATGAGATCCTCCAGGGA
TCCCTGCGGGATCAGTTTGTGGTGGATGTGTACCAGGCGGGGGCGGGCACCTCCCACCACATGAACGTCAACGAAGTGCT
GGCCAACCGCGCTCTGGAGATCCTGGGGGAGCCCAAGGGCCGCTACGAGCGGGTCAGCCCCAACGACCATGTCAACTACG
GCCAGTCCACCAACGATGTCATCCCCACCGCCATTCGTCTGGCTGCCCTGCTGCGGGTGCGACGGGATTTTCTGGGCACG
CTGGACAATTTGGTAGCGGCGCTGGAGCAGAAGGCCCAAGAGTTTCGCGGCATCCTCAAGGCCGGTCGCACCCATTTGCA
GGATGCGGTGCCGGTGCGGCTGGGGGATAGCTTCGGCGCCTACGCGGCCATCTTTGCCCAACACCGTCGCTGGATTGAGG
AGGCCAGCCAGGCCCTGTGCAGCTTGGGGCTGGGGGGAAGCGCCGTCGGCACCGGCCTCAACACTCATCCCCAGTACCGG
CAAAAGGTGGTGCAACGGCTGGCAGAGTATACCCAGTTGCCCCTCACCCCTGCTCCCCACCCCATGGCGGCGATGCAGAG
CATGGCCCCCTTTGTGCATCTGTCGGGATCCCTGCGCAACCTGGCCCAAGATGGCATCAAGCTGGCCAACGACCTGCGGC
TGATGGATTCTGGCCCCAAGACCGGCCTGAAGGAGATCCAACTGCCGCCGGTGCAGCCCGGCTCTTCCATCATGCCTGGC
AAGTACAACCCGGTGATAGCCGAGATGTTGAACATGGTCTGTTTCCAGGTGATTGGGCTGGATACCGCCATCACCCTCGC
AGCCCAAGCGGGGCAATTTGAGCTCAACGTGATGATGCCCCTGATTGCCTATGACCTCCTGCACAGCCTGCAGATTCTGA
ACAGCAGCCTGCGGGTCTTTGCCGAGCGCTGTATTCAAGGCATCACCCCCAAACCGGAGCGCTGCCGCGGCTACGCGGAA
GGGACGCTGGCTTTGGTGACCGCCCTCAACCCCTACATCGGCTATTTGCAAGCTGCAGAAGTGGCCAAGGCTTCTCTGGA
AACCGGCCAGCCCATTCGACAGATTGTTTTGGAGCGCAACCTCATGACCCCAGAGCAGGTGGCGGAAGTGCTGGATCTGG
AAGCCATGAGCCAAATGCAGTCCTAA

Upstream 100 bases:

>100_bases
CTTTACAAAACTTATCTGCAGCCGTCGAGACACCCTCAGAGAAGGCTGCCGGAAGCTAGCCCAAGGATCCGCGAACGGGG
GAGGAAAAGCCTTGGCTGAG

Downstream 100 bases:

>100_bases
CCCATTCGCTACAGTGGAGGCATACTGCTCCTGCCTGGCCATGACCATCGCCCCCTATCGCTGGCCCACCCAGCACGACT
TGCCCGATACAGACGACACC

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRSERDSMGERQLPQSVYYGIQTARALENFPISGLKPLPEYVEACVLIKKAAAEVNGELGCIPAEVAKAIVQAADEILQG
SLRDQFVVDVYQAGAGTSHHMNVNEVLANRALEILGEPKGRYERVSPNDHVNYGQSTNDVIPTAIRLAALLRVRRDFLGT
LDNLVAALEQKAQEFRGILKAGRTHLQDAVPVRLGDSFGAYAAIFAQHRRWIEEASQALCSLGLGGSAVGTGLNTHPQYR
QKVVQRLAEYTQLPLTPAPHPMAAMQSMAPFVHLSGSLRNLAQDGIKLANDLRLMDSGPKTGLKEIQLPPVQPGSSIMPG
KYNPVIAEMLNMVCFQVIGLDTAITLAAQAGQFELNVMMPLIAYDLLHSLQILNSSLRVFAERCIQGITPKPERCRGYAE
GTLALVTALNPYIGYLQAAEVAKASLETGQPIRQIVLERNLMTPEQVAEVLDLEAMSQMQS

Sequences:

>Translated_461_residues
MRSERDSMGERQLPQSVYYGIQTARALENFPISGLKPLPEYVEACVLIKKAAAEVNGELGCIPAEVAKAIVQAADEILQG
SLRDQFVVDVYQAGAGTSHHMNVNEVLANRALEILGEPKGRYERVSPNDHVNYGQSTNDVIPTAIRLAALLRVRRDFLGT
LDNLVAALEQKAQEFRGILKAGRTHLQDAVPVRLGDSFGAYAAIFAQHRRWIEEASQALCSLGLGGSAVGTGLNTHPQYR
QKVVQRLAEYTQLPLTPAPHPMAAMQSMAPFVHLSGSLRNLAQDGIKLANDLRLMDSGPKTGLKEIQLPPVQPGSSIMPG
KYNPVIAEMLNMVCFQVIGLDTAITLAAQAGQFELNVMMPLIAYDLLHSLQILNSSLRVFAERCIQGITPKPERCRGYAE
GTLALVTALNPYIGYLQAAEVAKASLETGQPIRQIVLERNLMTPEQVAEVLDLEAMSQMQS
>Mature_461_residues
MRSERDSMGERQLPQSVYYGIQTARALENFPISGLKPLPEYVEACVLIKKAAAEVNGELGCIPAEVAKAIVQAADEILQG
SLRDQFVVDVYQAGAGTSHHMNVNEVLANRALEILGEPKGRYERVSPNDHVNYGQSTNDVIPTAIRLAALLRVRRDFLGT
LDNLVAALEQKAQEFRGILKAGRTHLQDAVPVRLGDSFGAYAAIFAQHRRWIEEASQALCSLGLGGSAVGTGLNTHPQYR
QKVVQRLAEYTQLPLTPAPHPMAAMQSMAPFVHLSGSLRNLAQDGIKLANDLRLMDSGPKTGLKEIQLPPVQPGSSIMPG
KYNPVIAEMLNMVCFQVIGLDTAITLAAQAGQFELNVMMPLIAYDLLHSLQILNSSLRVFAERCIQGITPKPERCRGYAE
GTLALVTALNPYIGYLQAAEVAKASLETGQPIRQIVLERNLMTPEQVAEVLDLEAMSQMQS

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=459, Percent_Identity=42.7015250544662, Blast_Score=354, Evalue=9e-98,
Organism=Escherichia coli, GI87082375, Length=459, Percent_Identity=45.9694989106754, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1787896, Length=429, Percent_Identity=44.988344988345, Blast_Score=377, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17553882, Length=459, Percent_Identity=42.7015250544662, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32565146, Length=322, Percent_Identity=40.3726708074534, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324993, Length=459, Percent_Identity=41.8300653594771, Blast_Score=356, Evalue=5e-99,
Organism=Drosophila melanogaster, GI24640179, Length=460, Percent_Identity=41.7391304347826, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI24640177, Length=460, Percent_Identity=41.7391304347826, Blast_Score=358, Evalue=5e-99,
Organism=Drosophila melanogaster, GI24662684, Length=436, Percent_Identity=41.5137614678899, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI78710009, Length=461, Percent_Identity=39.0455531453362, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI24583245, Length=438, Percent_Identity=38.1278538812785, Blast_Score=330, Evalue=1e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR005677
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 50226; Mature: 50226

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSERDSMGERQLPQSVYYGIQTARALENFPISGLKPLPEYVEACVLIKKAAAEVNGELG
CCCCHHCCCHHHCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
CIPAEVAKAIVQAADEILQGSLRDQFVVDVYQAGAGTSHHMNVNEVLANRALEILGEPKG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
RYERVSPNDHVNYGQSTNDVIPTAIRLAALLRVRRDFLGTLDNLVAALEQKAQEFRGILK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRTHLQDAVPVRLGDSFGAYAAIFAQHRRWIEEASQALCSLGLGGSAVGTGLNTHPQYR
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHH
QKVVQRLAEYTQLPLTPAPHPMAAMQSMAPFVHLSGSLRNLAQDGIKLANDLRLMDSGPK
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCC
TGLKEIQLPPVQPGSSIMPGKYNPVIAEMLNMVCFQVIGLDTAITLAAQAGQFELNVMMP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEHHHHHH
LIAYDLLHSLQILNSSLRVFAERCIQGITPKPERCRGYAEGTLALVTALNPYIGYLQAAE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VAKASLETGQPIRQIVLERNLMTPEQVAEVLDLEAMSQMQS
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRSERDSMGERQLPQSVYYGIQTARALENFPISGLKPLPEYVEACVLIKKAAAEVNGELG
CCCCHHCCCHHHCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
CIPAEVAKAIVQAADEILQGSLRDQFVVDVYQAGAGTSHHMNVNEVLANRALEILGEPKG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCC
RYERVSPNDHVNYGQSTNDVIPTAIRLAALLRVRRDFLGTLDNLVAALEQKAQEFRGILK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGRTHLQDAVPVRLGDSFGAYAAIFAQHRRWIEEASQALCSLGLGGSAVGTGLNTHPQYR
HHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHH
QKVVQRLAEYTQLPLTPAPHPMAAMQSMAPFVHLSGSLRNLAQDGIKLANDLRLMDSGPK
HHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHCHHHHHHHHHHHHHHHHHHHHCCCCC
TGLKEIQLPPVQPGSSIMPGKYNPVIAEMLNMVCFQVIGLDTAITLAAQAGQFELNVMMP
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHCCCEEHHHHHH
LIAYDLLHSLQILNSSLRVFAERCIQGITPKPERCRGYAEGTLALVTALNPYIGYLQAAE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
VAKASLETGQPIRQIVLERNLMTPEQVAEVLDLEAMSQMQS
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1711029; 8969508; 9384377 [H]