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The map label for this gene is nolF [H]

Identifier: 86361015

GI number: 86361015

Start: 317572

End: 318657

Strand: Reverse

Name: nolF [H]

Synonym: RHE_PF00285

Alternate gene names: 86361015

Gene position: 318657-317572 (Counterclockwise)

Preceding gene: 86361016

Following gene: 86361014

Centisome position: 49.6

GC content: 65.75

Gene sequence:

>1086_bases
GTGAGTATTCGTCCCAAGATAATCGCACTGATGATGGGTACGGCCCTGATCTCCTCTTGCACGAAGCAGGAGGAAAGCAC
TGGTGAAGCGCCCCGTCCCGTGCTGTCGATGACAGTTAGGCAGACGCCTGCAACCAGCCTCGGCCTGACCGGCACAATCG
AGCCGACGATCGAGACCGAACTCGGCTTCCGGATCCTCGGGCGGATGATCGCCCGCAATGTCAATGTCGGCGACGTCGTC
CAGAAGGGCGATGTCGTTGCCGCCATCGATCCGCTGGCGCTGGAACTTGCCGTGCGCAGCGCCCAATCCGACGTCGAGAA
CAGCGACGCCCAGCTTCGAAACGCGGTGACGACGGAACAGCGCCAGCGCGCGCTGGTGGAATCGCGCTCGGGCACGGAGG
CCTCGCTCGAAGAGGCCGAACAGGCGAGACGGACGGCCGCCGCCGCCGTCGCCAAGGCGCAGGCCAATCTCGACAAGGCC
AAGGAGCAGCTCGGCTATGCGCAGCTTAAGGCGGAATTCGATGGCGTCGTAACGGCAACCTCGGCCGAGGTCGGCCAGGT
CGTGTCGGCCGGCCAGACGGTCGTCACGATAGCCCGGCCGGACAAGCGTGACGCCGTTGTCGACGTGCCCCAGGCCGCTG
CCCAGAAACTGAAGATCGGCGCCCCCTTCGAAGTGACGCTGCAGCTCGAGCCTTCGATCCGCACGACGGGCGTCGTGCGC
GAGATCGCGCCGGAGGCGGAGACCGCCACCCGCACGAGCAGGACCAAGATCGCGCTCGCCGATCCGCCTGAAGCCTTCAG
GCTCGGCGCGGTCATCACAGCCTCCGCAACAATCGCTGCCGATCCCGAGATCGTGCTGCCGTCCTCGGCGATCCTTGCCG
GCACCGACGGCGCCAGCGTCTGGATCGTGGACGTAGCAGGCAAGAAGGTCTCGCGCCGCCATGTGAGCATCGACGGCGAC
GTGTCCAATGGCGGCACCGTCCGCGTCACCGAAGGGCTCGCTCCTGGAGAGAGGGTCGTCGTGGCAGGCGTGCATAAACT
TGAGGATGACCAAGCCATCAGGATCGACCAGGAGATCAGCCAGTGA

Upstream 100 bases:

>100_bases
TCGTCAAGTCGGGCGTGTCGGCAGGCGACGTCGTGGTGACCGACGGCACCAAATTCCTCAGACCCGGTGAAATCGTGTCT
TACGTCAAGGAAGCATCCAA

Downstream 100 bases:

>100_bases
AGTCCTTCAATCTTTCCGACTGGGCGCTCGAGCATCGTTCGCTCGTCTGGTACTTCATGATCGTGTTCATTCTCGCCGGC
GCCTTCTCCTACCTGAACCT

Product: HlyD family protein secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSIRPKIIALMMGTALISSCTKQEESTGEAPRPVLSMTVRQTPATSLGLTGTIEPTIETELGFRILGRMIARNVNVGDVV
QKGDVVAAIDPLALELAVRSAQSDVENSDAQLRNAVTTEQRQRALVESRSGTEASLEEAEQARRTAAAAVAKAQANLDKA
KEQLGYAQLKAEFDGVVTATSAEVGQVVSAGQTVVTIARPDKRDAVVDVPQAAAQKLKIGAPFEVTLQLEPSIRTTGVVR
EIAPEAETATRTSRTKIALADPPEAFRLGAVITASATIAADPEIVLPSSAILAGTDGASVWIVDVAGKKVSRRHVSIDGD
VSNGGTVRVTEGLAPGERVVVAGVHKLEDDQAIRIDQEISQ

Sequences:

>Translated_361_residues
MSIRPKIIALMMGTALISSCTKQEESTGEAPRPVLSMTVRQTPATSLGLTGTIEPTIETELGFRILGRMIARNVNVGDVV
QKGDVVAAIDPLALELAVRSAQSDVENSDAQLRNAVTTEQRQRALVESRSGTEASLEEAEQARRTAAAAVAKAQANLDKA
KEQLGYAQLKAEFDGVVTATSAEVGQVVSAGQTVVTIARPDKRDAVVDVPQAAAQKLKIGAPFEVTLQLEPSIRTTGVVR
EIAPEAETATRTSRTKIALADPPEAFRLGAVITASATIAADPEIVLPSSAILAGTDGASVWIVDVAGKKVSRRHVSIDGD
VSNGGTVRVTEGLAPGERVVVAGVHKLEDDQAIRIDQEISQ
>Mature_360_residues
SIRPKIIALMMGTALISSCTKQEESTGEAPRPVLSMTVRQTPATSLGLTGTIEPTIETELGFRILGRMIARNVNVGDVVQ
KGDVVAAIDPLALELAVRSAQSDVENSDAQLRNAVTTEQRQRALVESRSGTEASLEEAEQARRTAAAAVAKAQANLDKAK
EQLGYAQLKAEFDGVVTATSAEVGQVVSAGQTVVTIARPDKRDAVVDVPQAAAQKLKIGAPFEVTLQLEPSIRTTGVVRE
IAPEAETATRTSRTKIALADPPEAFRLGAVITASATIAADPEIVLPSSAILAGTDGASVWIVDVAGKKVSRRHVSIDGDV
SNGGTVRVTEGLAPGERVVVAGVHKLEDDQAIRIDQEISQ

Specific function: Involved in the production of Medicago-specific nodulation signal molecule [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=319, Percent_Identity=26.6457680250784, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789929, Length=363, Percent_Identity=23.1404958677686, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37877; Mature: 37746

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRPKIIALMMGTALISSCTKQEESTGEAPRPVLSMTVRQTPATSLGLTGTIEPTIETE
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCCCCCCCEEECCCCCHH
LGFRILGRMIARNVNVGDVVQKGDVVAAIDPLALELAVRSAQSDVENSDAQLRNAVTTEQ
HHHHHHHHHHHCCCCHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RQRALVESRSGTEASLEEAEQARRTAAAAVAKAQANLDKAKEQLGYAQLKAEFDGVVTAT
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEEC
SAEVGQVVSAGQTVVTIARPDKRDAVVDVPQAAAQKLKIGAPFEVTLQLEPSIRTTGVVR
CHHHHHHHHCCCEEEEEECCCCCCCEEECCHHHHHHEECCCCEEEEEEECCCCCHHHHHH
EIAPEAETATRTSRTKIALADPPEAFRLGAVITASATIAADPEIVLPSSAILAGTDGASV
HHCCCCHHHHCCCCCEEEECCCCCHHEECEEEEECEEEECCCCEECCCCEEEECCCCCEE
WIVDVAGKKVSRRHVSIDGDVSNGGTVRVTEGLAPGERVVVAGVHKLEDDQAIRIDQEIS
EEEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCEEECCHHHC
Q
C
>Mature Secondary Structure 
SIRPKIIALMMGTALISSCTKQEESTGEAPRPVLSMTVRQTPATSLGLTGTIEPTIETE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHEEEECCCCCCCCCCCEEECCCCCHH
LGFRILGRMIARNVNVGDVVQKGDVVAAIDPLALELAVRSAQSDVENSDAQLRNAVTTEQ
HHHHHHHHHHHCCCCHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
RQRALVESRSGTEASLEEAEQARRTAAAAVAKAQANLDKAKEQLGYAQLKAEFDGVVTAT
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEEEC
SAEVGQVVSAGQTVVTIARPDKRDAVVDVPQAAAQKLKIGAPFEVTLQLEPSIRTTGVVR
CHHHHHHHHCCCEEEEEECCCCCCCEEECCHHHHHHEECCCCEEEEEEECCCCCHHHHHH
EIAPEAETATRTSRTKIALADPPEAFRLGAVITASATIAADPEIVLPSSAILAGTDGASV
HHCCCCHHHHCCCCCEEEECCCCCHHEECEEEEECEEEECCCCEECCCCEEEECCCCCEE
WIVDVAGKKVSRRHVSIDGDVSNGGTVRVTEGLAPGERVVVAGVHKLEDDQAIRIDQEIS
EEEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCEEECCHHHC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1909418; 11481432 [H]