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The map label for this gene is glnH

Identifier: 86360985

GI number: 86360985

Start: 273042

End: 273869

Strand: Reverse

Name: glnH

Synonym: RHE_PF00255

Alternate gene names: 86360985

Gene position: 273869-273042 (Counterclockwise)

Preceding gene: 86360987

Following gene: 86360984

Centisome position: 42.62

GC content: 59.78

Gene sequence:

>828_bases
ATGAAGATTTTTGAATCACTGCCGGCAGTCATTGCGCCTCTGCAAGCCGGCCTCGCGGCTTGCTTTCTGGCGATGGGAGC
GGGTTTCGCAGCGGCGGCCGACAATCCCTATAATCTGATCGAGCCCGACGTCATCAGTGTCGGCACGATGGGCGATGCCA
AGCCCTATACATTTGCGACCGCGGATGGCCAGTTCACCGGTTTCGACATCGAACTATTTCTCAACGTCGTGTCTCGTCTC
GGCTTTGCAAAGGACAAGGTGACGTTCACCGGCCAGGAATTTTCTGCGCTCCTGCCATCGGTTGCAAACGAGCGGTTCGA
CGTTGCCGTCGCTGCGATCGGAACCACCGAAGCCCGCGAGAAGACCGTCGATTTCTCCGACGGCTATCTCGCCGGTTATC
TGTCCGTGCTGACGCCGGACGCGGCCATCAAGGACGCGGCCGGGCTGAAGGGCAAGCGGCTCGGCGTCGTGCAGGGAACC
TTGCAGGAAGTCTACGCCGCCAAGAATTTTGGCGAGACCGATCTCGTGAAATTTCCCGACAACAATTCCGCCGTCGCCGC
ACTCAACAACGGCACGATCGACGCGCATTTCCTCGACTACGAAGCGGCCAAGCAATATGGCGAGCGCTATCCCGCGCTGA
AGGTCGCCGTCAACATTCCGTCCTTCGATGCGCCGGCGGGCTTCGTGGTCAGGCAGGGAAACGATGCCTTCCGCACGGCG
TTGAACGGCGCCCTTCATGACGCGATGCAGGACGGCACCTGGAAGACGCTCTATGAGAAGTGGTTCCCCGGCTCACCGAT
GCCGGATCAATATCTCCCCAAGAAATGA

Upstream 100 bases:

>100_bases
AGACCGGTATCATTGCGGGCCGACTTGCCGCGAAACGGCGGCAGGGGCGGATAAACAGCTAATGCCGACAAGAGCATTCC
TTCCGGAGCGGAGAACGAAC

Downstream 100 bases:

>100_bases
GCCTACGGCCGTCCGCCGACCGCGGACGGCCGAGTCTCGGCGGTTGGCGTACCCGGCAAGGGTTTCATGGGGATCCCGAT
GAACTGGATAGACAATCTGC

Product: glutamine ABC transporter, substrate binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKIFESLPAVIAPLQAGLAACFLAMGAGFAAAADNPYNLIEPDVISVGTMGDAKPYTFATADGQFTGFDIELFLNVVSRL
GFAKDKVTFTGQEFSALLPSVANERFDVAVAAIGTTEAREKTVDFSDGYLAGYLSVLTPDAAIKDAAGLKGKRLGVVQGT
LQEVYAAKNFGETDLVKFPDNNSAVAALNNGTIDAHFLDYEAAKQYGERYPALKVAVNIPSFDAPAGFVVRQGNDAFRTA
LNGALHDAMQDGTWKTLYEKWFPGSPMPDQYLPKK

Sequences:

>Translated_275_residues
MKIFESLPAVIAPLQAGLAACFLAMGAGFAAAADNPYNLIEPDVISVGTMGDAKPYTFATADGQFTGFDIELFLNVVSRL
GFAKDKVTFTGQEFSALLPSVANERFDVAVAAIGTTEAREKTVDFSDGYLAGYLSVLTPDAAIKDAAGLKGKRLGVVQGT
LQEVYAAKNFGETDLVKFPDNNSAVAALNNGTIDAHFLDYEAAKQYGERYPALKVAVNIPSFDAPAGFVVRQGNDAFRTA
LNGALHDAMQDGTWKTLYEKWFPGSPMPDQYLPKK
>Mature_275_residues
MKIFESLPAVIAPLQAGLAACFLAMGAGFAAAADNPYNLIEPDVISVGTMGDAKPYTFATADGQFTGFDIELFLNVVSRL
GFAKDKVTFTGQEFSALLPSVANERFDVAVAAIGTTEAREKTVDFSDGYLAGYLSVLTPDAAIKDAAGLKGKRLGVVQGT
LQEVYAAKNFGETDLVKFPDNNSAVAALNNGTIDAHFLDYEAAKQYGERYPALKVAVNIPSFDAPAGFVVRQGNDAFRTA
LNGALHDAMQDGTWKTLYEKWFPGSPMPDQYLPKK

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=223, Percent_Identity=30.0448430493274, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788228, Length=252, Percent_Identity=28.968253968254, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1787088, Length=243, Percent_Identity=28.3950617283951, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=28.936170212766, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788649, Length=253, Percent_Identity=28.0632411067194, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787031, Length=219, Percent_Identity=28.7671232876712, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29386; Mature: 29386

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFESLPAVIAPLQAGLAACFLAMGAGFAAAADNPYNLIEPDVISVGTMGDAKPYTFAT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCEEEECCCCCCCCCEEEE
ADGQFTGFDIELFLNVVSRLGFAKDKVTFTGQEFSALLPSVANERFDVAVAAIGTTEARE
CCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHCCCCEEEEEEEECCCHHHH
KTVDFSDGYLAGYLSVLTPDAAIKDAAGLKGKRLGVVQGTLQEVYAAKNFGETDLVKFPD
HCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCCCEEECCC
NNSAVAALNNGTIDAHFLDYEAAKQYGERYPALKVAVNIPSFDAPAGFVVRQGNDAFRTA
CCCEEEEECCCEEEEEECCHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEECCCHHHHHH
LNGALHDAMQDGTWKTLYEKWFPGSPMPDQYLPKK
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIFESLPAVIAPLQAGLAACFLAMGAGFAAAADNPYNLIEPDVISVGTMGDAKPYTFAT
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCEEEECCCCCCCCCEEEE
ADGQFTGFDIELFLNVVSRLGFAKDKVTFTGQEFSALLPSVANERFDVAVAAIGTTEARE
CCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHCCCCEEEEEEEECCCHHHH
KTVDFSDGYLAGYLSVLTPDAAIKDAAGLKGKRLGVVQGTLQEVYAAKNFGETDLVKFPD
HCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCEEHHHHHHHHHHHHHCCCCCCCEEECCC
NNSAVAALNNGTIDAHFLDYEAAKQYGERYPALKVAVNIPSFDAPAGFVVRQGNDAFRTA
CCCEEEEECCCEEEEEECCHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEECCCHHHHHH
LNGALHDAMQDGTWKTLYEKWFPGSPMPDQYLPKK
HHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]