Definition Rhizobium etli CFN 42 plasmid p42f, complete sequence.
Accession NC_007766
Length 642,517

Click here to switch to the map view.

The map label for this gene is rbsB [H]

Identifier: 86360948

GI number: 86360948

Start: 230207

End: 231169

Strand: Reverse

Name: rbsB [H]

Synonym: RHE_PF00217

Alternate gene names: 86360948

Gene position: 231169-230207 (Counterclockwise)

Preceding gene: 86360950

Following gene: 86360947

Centisome position: 35.98

GC content: 57.74

Gene sequence:

>963_bases
ATGAAACAGCTGAAACGGAATGCAGCCTTGTTCCTTGCCGGGCTGATACTGAGCGCGGCGCCGATTGCAGTATCCGATGC
CGCCGACAAACCGACTCTTGCTTTCGTCGTCAACGGAGCGTCTGACTTCTGGAAAGCTGCGGAAGCGGGCGTGAAGAAGG
CCCAGGCCGAATTGCCGGATTACCAACTGGAGCTGAAGTACCCTGAGCAGGCATCGGTAGCCATCCAGCAGCGCCTGATG
GAAGACCTAGTCAGTGCTGGCGTAAAGGGGATCATGGTTTCCGCAGTCGACCCCAAAACTCAGACCGAGGGCTTGAACAA
GATCGGCTCACAAACAGCTCTCTTCACGACCGACAGTGACGCGCCGCAGACCAACCGCGTCGCTTATATCGGCTCATCCA
ATATCGACGCCGGTCTGCAGGCGGCCGAAATCGCGAAGAAGGCAATGCCGGATGGCGGCAAGTGCATCGGTTTTGTCGGG
CTCCTCGGAGCCGATAATGCCAAGGAACGCATTCAGGGCATGAAGGATGGTCTTAAGGGCACGAAGATCGAGCTGACAGA
TGTGCGCGGCGACGATATCGACCAGACGCGCGCAAAAAAGAATGTCGAGGATGCACTGGTGGCCAGTCCGGACGTGACCT
GCATGGTCGGCTTCTACTCTTATAACACGCCACGCATCTATGAGGCGCTGCGCGACGCCGGAAAGCTCGGCCAGATCACT
GTCGTCGGCTTTGACGACGATCCGATCACGCTCGGCGGCGTCAAAGAAGGCACGGTCGCGGCAACCGTCGTGCAGCAGCC
ATTCGAATGGGCTTATCAGGGGATGAAGCTGATGGCCGCCTACCTCAAGGGCGACAAGTCCGGTGTTCCCAGCAACGGCT
TGATCATCATTCCGACAGTGATCATCGGCAAGGATGACGTCGACAAATATGCCGCTAACCTGAAGGCGATGGCTGGAAAG
TAA

Upstream 100 bases:

>100_bases
CACTCGTCGCTTTTGCGGCGGCCCTAGGAGGGGGCGCGCATTCGGCCGGCCGCAATTCGCAAACGGGGCCATGCGACTGC
GGCCCCTCGGGAGGTATATT

Downstream 100 bases:

>100_bases
TTCTCGTTCGCGGCGGGGGTACTCCCCGCCGCGAAAGACGTTTCATGCCGCAGCACCCGCAGTCATGGACAGGCAATGAA
TCATAGCTCAGACATCCCGT

Product: sugar ABC transporter substrate-binding protein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKQLKRNAALFLAGLILSAAPIAVSDAADKPTLAFVVNGASDFWKAAEAGVKKAQAELPDYQLELKYPEQASVAIQQRLM
EDLVSAGVKGIMVSAVDPKTQTEGLNKIGSQTALFTTDSDAPQTNRVAYIGSSNIDAGLQAAEIAKKAMPDGGKCIGFVG
LLGADNAKERIQGMKDGLKGTKIELTDVRGDDIDQTRAKKNVEDALVASPDVTCMVGFYSYNTPRIYEALRDAGKLGQIT
VVGFDDDPITLGGVKEGTVAATVVQQPFEWAYQGMKLMAAYLKGDKSGVPSNGLIIIPTVIIGKDDVDKYAANLKAMAGK

Sequences:

>Translated_320_residues
MKQLKRNAALFLAGLILSAAPIAVSDAADKPTLAFVVNGASDFWKAAEAGVKKAQAELPDYQLELKYPEQASVAIQQRLM
EDLVSAGVKGIMVSAVDPKTQTEGLNKIGSQTALFTTDSDAPQTNRVAYIGSSNIDAGLQAAEIAKKAMPDGGKCIGFVG
LLGADNAKERIQGMKDGLKGTKIELTDVRGDDIDQTRAKKNVEDALVASPDVTCMVGFYSYNTPRIYEALRDAGKLGQIT
VVGFDDDPITLGGVKEGTVAATVVQQPFEWAYQGMKLMAAYLKGDKSGVPSNGLIIIPTVIIGKDDVDKYAANLKAMAGK
>Mature_320_residues
MKQLKRNAALFLAGLILSAAPIAVSDAADKPTLAFVVNGASDFWKAAEAGVKKAQAELPDYQLELKYPEQASVAIQQRLM
EDLVSAGVKGIMVSAVDPKTQTEGLNKIGSQTALFTTDSDAPQTNRVAYIGSSNIDAGLQAAEIAKKAMPDGGKCIGFVG
LLGADNAKERIQGMKDGLKGTKIELTDVRGDDIDQTRAKKNVEDALVASPDVTCMVGFYSYNTPRIYEALRDAGKLGQIT
VVGFDDDPITLGGVKEGTVAATVVQQPFEWAYQGMKLMAAYLKGDKSGVPSNGLIIIPTVIIGKDDVDKYAANLKAMAGK

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=290, Percent_Identity=28.6206896551724, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33833; Mature: 33833

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLKRNAALFLAGLILSAAPIAVSDAADKPTLAFVVNGASDFWKAAEAGVKKAQAELPD
CCCHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCC
YQLELKYPEQASVAIQQRLMEDLVSAGVKGIMVSAVDPKTQTEGLNKIGSQTALFTTDSD
EEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCC
APQTNRVAYIGSSNIDAGLQAAEIAKKAMPDGGKCIGFVGLLGADNAKERIQGMKDGLKG
CCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCC
TKIELTDVRGDDIDQTRAKKNVEDALVASPDVTCMVGFYSYNTPRIYEALRDAGKLGQIT
CEEEEEECCCCCCHHHHHHCCHHHHHHCCCCCEEEEEEEECCCHHHHHHHHCCCCCCEEE
VVGFDDDPITLGGVKEGTVAATVVQQPFEWAYQGMKLMAAYLKGDKSGVPSNGLIIIPTV
EEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
IIGKDDVDKYAANLKAMAGK
EECCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKQLKRNAALFLAGLILSAAPIAVSDAADKPTLAFVVNGASDFWKAAEAGVKKAQAELPD
CCCHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCC
YQLELKYPEQASVAIQQRLMEDLVSAGVKGIMVSAVDPKTQTEGLNKIGSQTALFTTDSD
EEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCC
APQTNRVAYIGSSNIDAGLQAAEIAKKAMPDGGKCIGFVGLLGADNAKERIQGMKDGLKG
CCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHCCCCC
TKIELTDVRGDDIDQTRAKKNVEDALVASPDVTCMVGFYSYNTPRIYEALRDAGKLGQIT
CEEEEEECCCCCCHHHHHHCCHHHHHHCCCCCEEEEEEEECCCHHHHHHHHCCCCCCEEE
VVGFDDDPITLGGVKEGTVAATVVQQPFEWAYQGMKLMAAYLKGDKSGVPSNGLIIIPTV
EEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEE
IIGKDDVDKYAANLKAMAGK
EECCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]