Definition Rhizobium etli CFN 42 plasmid p42f, complete sequence.
Accession NC_007766
Length 642,517

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The map label for this gene is repAf2

Identifier: 86360945

GI number: 86360945

Start: 225829

End: 227022

Strand: Reverse

Name: repAf2

Synonym: RHE_PF00214

Alternate gene names: 86360945

Gene position: 227022-225829 (Counterclockwise)

Preceding gene: 86360946

Following gene: 86360944

Centisome position: 35.33

GC content: 61.06

Gene sequence:

>1194_bases
ATGCAGCCGAGTCTTGCTCTTCAAGATGATCAAGAGCACCTTCCGTCGCTTCTGGCAACGGATGCGAAGGAGTTGTCCTA
TCAACTTCAGCAGCATCAGGCCAAAATCTTTCCTCCGCTATCGCAAAAGACGATCAGGACATTTTCTCCGGCAGAGGCCG
CGGCCTTCATCGGCATCGGCGAAGGTTATCTCCGGCAGGTGGCGGCCGACGGCCATGGTCCGGATCCGCTGGCAAACGGA
CGACGGCTCTATAGCGCAACCGACATGGATCGGATCCGCCGCGTCCTCGACGAGCGGAACGGAACGCCGAAATATGTGCC
GGCCCGCAGGCCGGGCGAAAAGCTCCAGATCGTCTCCGTGATGAATTTCAAGGGTGGGTCGGGCAAGACCACGACCGCGG
CCCATTTTGCGCAGTTCATGGCGCTCCGGGGCTACCGCGTGCTTGCCGTCGACCTCGACCCGCAGGCCTCCTTGTCCGCG
CTGTTCGGCCATCAGCCGGAATTCGACGTCGGCGAGGGCGAAACGATCTACGGCGCAATCCGCTATGACGATCCGCGCCC
GATCGCCGATATCGTGCGCGCCACCTATACGCCGAACCTGCATCTCATTCCCGGCAATCTGGAATTGATGGAATTCGAGC
ACGAGACGCCGAAGGCCATGGCATCGGGCACGGCGGAGACGATGTTCTTTGCCCGTATCGGCGAGGTGCTGACCGATATC
GAAAGCCTCTACGACATCGTCGTCGTCGACTGCCCGCCGCAGCTCGGCTTCCTGACGATGTCGGCGCTCTGCGCGGCGAC
TTCGGTGTTGATCACCGTTCACCCGCAGATGCTCGACGTCATGTCGATGTCGCAGTTCCTGACGATGACGAGCGAACTGA
TGTCGGTCGTCGAGAGGGCCGGCGGGCGCACCAGCTATGACTGGATGCGCTATCTCGTGACCCGGTTCGAGCCGAATGAC
GGGCCGCAGAGCCAGATGACCGGCTTCATGCGGGCGATCTTCGGCAATCGCATGCTCCACAATGCCATGGTGAAATCGAC
GGCCGTTTCCGATGCCGGCGTCACCAAGCAGACACTCTACGAAGTCGAGCGGTCGCAGTTCACGCGCGGAACTTATGATC
GGGCGCTGGAGTCGCTGAACCTCGTGAATGGCGAGATCGAAGCGCATATTCGCTCCACCTGGGGAAGGAGATAG

Upstream 100 bases:

>100_bases
TGGTTAAGCATTTGTAATTCGGTCACGAATCGCGGCATATAGTAACTGCGCAACTGAAGCGCTTTCGTTCAAAATTGCGC
AGTTACGCAGGAAAGAGATC

Downstream 100 bases:

>100_bases
AAATGGCGCGCAAGAACCTCATCGAAATCTCCGCTCCGAGCGCGGCAAGGGTGGAAGCGATCGCCCCCCGCGACAATCGC
CCGATCGCCGGGTTCGTGCC

Product: plasmid partitioning protein RepAf2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MQPSLALQDDQEHLPSLLATDAKELSYQLQQHQAKIFPPLSQKTIRTFSPAEAAAFIGIGEGYLRQVAADGHGPDPLANG
RRLYSATDMDRIRRVLDERNGTPKYVPARRPGEKLQIVSVMNFKGGSGKTTTAAHFAQFMALRGYRVLAVDLDPQASLSA
LFGHQPEFDVGEGETIYGAIRYDDPRPIADIVRATYTPNLHLIPGNLELMEFEHETPKAMASGTAETMFFARIGEVLTDI
ESLYDIVVVDCPPQLGFLTMSALCAATSVLITVHPQMLDVMSMSQFLTMTSELMSVVERAGGRTSYDWMRYLVTRFEPND
GPQSQMTGFMRAIFGNRMLHNAMVKSTAVSDAGVTKQTLYEVERSQFTRGTYDRALESLNLVNGEIEAHIRSTWGRR

Sequences:

>Translated_397_residues
MQPSLALQDDQEHLPSLLATDAKELSYQLQQHQAKIFPPLSQKTIRTFSPAEAAAFIGIGEGYLRQVAADGHGPDPLANG
RRLYSATDMDRIRRVLDERNGTPKYVPARRPGEKLQIVSVMNFKGGSGKTTTAAHFAQFMALRGYRVLAVDLDPQASLSA
LFGHQPEFDVGEGETIYGAIRYDDPRPIADIVRATYTPNLHLIPGNLELMEFEHETPKAMASGTAETMFFARIGEVLTDI
ESLYDIVVVDCPPQLGFLTMSALCAATSVLITVHPQMLDVMSMSQFLTMTSELMSVVERAGGRTSYDWMRYLVTRFEPND
GPQSQMTGFMRAIFGNRMLHNAMVKSTAVSDAGVTKQTLYEVERSQFTRGTYDRALESLNLVNGEIEAHIRSTWGRR
>Mature_397_residues
MQPSLALQDDQEHLPSLLATDAKELSYQLQQHQAKIFPPLSQKTIRTFSPAEAAAFIGIGEGYLRQVAADGHGPDPLANG
RRLYSATDMDRIRRVLDERNGTPKYVPARRPGEKLQIVSVMNFKGGSGKTTTAAHFAQFMALRGYRVLAVDLDPQASLSA
LFGHQPEFDVGEGETIYGAIRYDDPRPIADIVRATYTPNLHLIPGNLELMEFEHETPKAMASGTAETMFFARIGEVLTDI
ESLYDIVVVDCPPQLGFLTMSALCAATSVLITVHPQMLDVMSMSQFLTMTSELMSVVERAGGRTSYDWMRYLVTRFEPND
GPQSQMTGFMRAIFGNRMLHNAMVKSTAVSDAGVTKQTLYEVERSQFTRGTYDRALESLNLVNGEIEAHIRSTWGRR

Specific function: This protein is coded by a hairy root Ri plasmid. It is possibly involved in regulating the plasmid copy-number [H]

COG id: COG1192

COG function: function code D; ATPases involved in chromosome partitioning

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017818 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: NA

Molecular weight: Translated: 44076; Mature: 44076

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPSLALQDDQEHLPSLLATDAKELSYQLQQHQAKIFPPLSQKTIRTFSPAEAAAFIGIG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHEEEECC
EGYLRQVAADGHGPDPLANGRRLYSATDMDRIRRVLDERNGTPKYVPARRPGEKLQIVSV
HHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
MNFKGGSGKTTTAAHFAQFMALRGYRVLAVDLDPQASLSALFGHQPEFDVGEGETIYGAI
EECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCEEEEEE
RYDDPRPIADIVRATYTPNLHLIPGNLELMEFEHETPKAMASGTAETMFFARIGEVLTDI
EECCCCCHHHHHHHHCCCCEEEECCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHH
ESLYDIVVVDCPPQLGFLTMSALCAATSVLITVHPQMLDVMSMSQFLTMTSELMSVVERA
HHHHCEEEEECCCCCHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHC
GGRTSYDWMRYLVTRFEPNDGPQSQMTGFMRAIFGNRMLHNAMVKSTAVSDAGVTKQTLY
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EVERSQFTRGTYDRALESLNLVNGEIEAHIRSTWGRR
HHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MQPSLALQDDQEHLPSLLATDAKELSYQLQQHQAKIFPPLSQKTIRTFSPAEAAAFIGIG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCHHHHEEEECC
EGYLRQVAADGHGPDPLANGRRLYSATDMDRIRRVLDERNGTPKYVPARRPGEKLQIVSV
HHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE
MNFKGGSGKTTTAAHFAQFMALRGYRVLAVDLDPQASLSALFGHQPEFDVGEGETIYGAI
EECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCCCCCCCCEEEEEE
RYDDPRPIADIVRATYTPNLHLIPGNLELMEFEHETPKAMASGTAETMFFARIGEVLTDI
EECCCCCHHHHHHHHCCCCEEEECCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHH
ESLYDIVVVDCPPQLGFLTMSALCAATSVLITVHPQMLDVMSMSQFLTMTSELMSVVERA
HHHHCEEEEECCCCCHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHC
GGRTSYDWMRYLVTRFEPNDGPQSQMTGFMRAIFGNRMLHNAMVKSTAVSDAGVTKQTLY
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
EVERSQFTRGTYDRALESLNLVNGEIEAHIRSTWGRR
HHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA