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The map label for this gene is thuE

Identifier: 86360941

GI number: 86360941

Start: 220709

End: 221974

Strand: Reverse

Name: thuE

Synonym: RHE_PF00210

Alternate gene names: 86360941

Gene position: 221974-220709 (Counterclockwise)

Preceding gene: 86360943

Following gene: 86360940

Centisome position: 34.55

GC content: 59.24

Gene sequence:

>1266_bases
ATGAAAAAGTTTGCTTTAGGCTTGTCGGCGCTCGGCCTGGCCATCGCCATCGCTGGTTCGATCCATGCCGCCGAAATTTC
ATTTGCCGCCAACACCACCGGGAAAAACGTCGAATTCCTCAATAAGCAGCTGGCGATTTTCGGGAAGGAGACCGGCAATC
AGGTCAAACTCGTCACCATGCCCTCGTCGAGCAGCGAGCAGTTCTCCCAATATCGCCTGTGGCTAGCCGCCGGAAACAAG
GACATCGACGTCTATCAGACCGACGTTATCTGGGCGCCGCAGCTTGCAGACCAGTTCATCGATCTGAAGGAAGCTACCAA
AGATGTAGCAGGCCAGTTCTTCCCGTCGATCATTGCCTCGCAGACGGTCAACGGCCGACTGGTGGCGCTGCCGCTGTTTA
CAGATGCTCCCGCGCTGTTTTACCGGAAGGACCTGCTTGAAAAATACGGCAAGCTGCCGCCAAAGACCTGGGACGAGATG
GCCGCGACCGCCAAGGAAGTTCAGGATAAGGAACGCCAGGCCGGACAGAAGGACCTTTGGGGCTACGTCTTCCAGGGCAA
CTCCTATGAAGGGCTGACCTGCAACGCGCTGGAATGGGTAAAGTCGTCGGGCGGCGGCCAGATTGTCGAGCCAGACGGGA
CAATTTCGATCAACAACGAGAAGGCGGCGGCAGCGCTTGAACGCGCCAAGGGCTGGATCGGAACGATCTCACCGCCGGGC
GTGCTCGCATATCAGGAAGAAGAATCGCGCGGTGTTTGGCAGACAGGCAACGCCGTCTTCATGCGCAACTGGCCCTATGC
CTATGCCCTCGGCAATGGTGCCGACAGTGCCGTGAAGGGCAAGTTCGACGTGATGACGCTGCCGGTTGCGGCCGCCGGGG
ACAAGCCATCCTCGACACTCGGCGGTTGGAACCTGGCGGTGTCGAAATATTCGGAAAAACAGGAAGCAGCCATCGCGCTC
GTCAAATTCCTGGCGTCGAAGGACGTGCAAAAGGCGCGCGCCATCGAGCTGTCCAATCTGCCGACACTGACCGACCTTTA
TGACGACAAGGATATTGCCGCCGCGCAGCCCTTCATGCCCAACTGGAAGCCTATTTTCCAGAACGCGGTGCCGCGTCCTT
CGGCGACCGCCAAGGTCAAGTACAACGAAGTGTCCTCGAAGTTCTGGACGGCCGTGCACAACACGCTGTCCGGCAGCGGC
TCGGCCGCCGAGAACCTCGAGCTTCTCGAAGCCGACCTGACGACCCTCAAGGGCGACGCCTGGTAA

Upstream 100 bases:

>100_bases
TAACTCACAACTGTGAAGGGTTCATTTGGCGGCTTGACGCCCTTCAAGCCTTCTGCAATGTTCTGTTGTCCAAATCGATT
TCAATTTCTGGGAGGAGATC

Downstream 100 bases:

>100_bases
GAACCGGGCCGGCGGCTGTGAAGCCGCCGGCCCGGTTTCGGACGAGCAACCGGGGAGGCGTTTCATGACTGAAATCGTAG
TTTCGCAAGCAGTTGAACCA

Product: trehalosemaltose ABC transporter, sugar-binding protein

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MKKFALGLSALGLAIAIAGSIHAAEISFAANTTGKNVEFLNKQLAIFGKETGNQVKLVTMPSSSSEQFSQYRLWLAAGNK
DIDVYQTDVIWAPQLADQFIDLKEATKDVAGQFFPSIIASQTVNGRLVALPLFTDAPALFYRKDLLEKYGKLPPKTWDEM
AATAKEVQDKERQAGQKDLWGYVFQGNSYEGLTCNALEWVKSSGGGQIVEPDGTISINNEKAAAALERAKGWIGTISPPG
VLAYQEEESRGVWQTGNAVFMRNWPYAYALGNGADSAVKGKFDVMTLPVAAAGDKPSSTLGGWNLAVSKYSEKQEAAIAL
VKFLASKDVQKARAIELSNLPTLTDLYDDKDIAAAQPFMPNWKPIFQNAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSG
SAAENLELLEADLTTLKGDAW

Sequences:

>Translated_421_residues
MKKFALGLSALGLAIAIAGSIHAAEISFAANTTGKNVEFLNKQLAIFGKETGNQVKLVTMPSSSSEQFSQYRLWLAAGNK
DIDVYQTDVIWAPQLADQFIDLKEATKDVAGQFFPSIIASQTVNGRLVALPLFTDAPALFYRKDLLEKYGKLPPKTWDEM
AATAKEVQDKERQAGQKDLWGYVFQGNSYEGLTCNALEWVKSSGGGQIVEPDGTISINNEKAAAALERAKGWIGTISPPG
VLAYQEEESRGVWQTGNAVFMRNWPYAYALGNGADSAVKGKFDVMTLPVAAAGDKPSSTLGGWNLAVSKYSEKQEAAIAL
VKFLASKDVQKARAIELSNLPTLTDLYDDKDIAAAQPFMPNWKPIFQNAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSG
SAAENLELLEADLTTLKGDAW
>Mature_421_residues
MKKFALGLSALGLAIAIAGSIHAAEISFAANTTGKNVEFLNKQLAIFGKETGNQVKLVTMPSSSSEQFSQYRLWLAAGNK
DIDVYQTDVIWAPQLADQFIDLKEATKDVAGQFFPSIIASQTVNGRLVALPLFTDAPALFYRKDLLEKYGKLPPKTWDEM
AATAKEVQDKERQAGQKDLWGYVFQGNSYEGLTCNALEWVKSSGGGQIVEPDGTISINNEKAAAALERAKGWIGTISPPG
VLAYQEEESRGVWQTGNAVFMRNWPYAYALGNGADSAVKGKFDVMTLPVAAAGDKPSSTLGGWNLAVSKYSEKQEAAIAL
VKFLASKDVQKARAIELSNLPTLTDLYDDKDIAAAQPFMPNWKPIFQNAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSG
SAAENLELLEADLTTLKGDAW

Specific function: Probably part of a binding-protein-dependent transport system [H]

COG id: COG1653

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

None

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 720 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 45603; Mature: 45603

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFALGLSALGLAIAIAGSIHAAEISFAANTTGKNVEFLNKQLAIFGKETGNQVKLVTM
CCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCHHHHHHEEEEEECCCCCEEEEEEC
PSSSSEQFSQYRLWLAAGNKDIDVYQTDVIWAPQLADQFIDLKEATKDVAGQFFPSIIAS
CCCCHHHHHHEEEEEEECCCCCEEEEECEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTVNGRLVALPLFTDAPALFYRKDLLEKYGKLPPKTWDEMAATAKEVQDKERQAGQKDLW
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GYVFQGNSYEGLTCNALEWVKSSGGGQIVEPDGTISINNEKAAAALERAKGWIGTISPPG
EEEEECCCCCCEECHHHHHHHCCCCCEEECCCCEEEECCHHHHHHHHHHCCCEECCCCCC
VLAYQEEESRGVWQTGNAVFMRNWPYAYALGNGADSAVKGKFDVMTLPVAAAGDKPSSTL
EEEEECCCCCCCEECCCEEEEECCCEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCCC
GGWNLAVSKYSEKQEAAIALVKFLASKDVQKARAIELSNLPTLTDLYDDKDIAAAQPFMP
CCEEEEEHHHCHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCHHHHCCCCCCCCCCCCCC
NWKPIFQNAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSGSAAENLELLEADLTTLKGDA
CCHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
W
C
>Mature Secondary Structure
MKKFALGLSALGLAIAIAGSIHAAEISFAANTTGKNVEFLNKQLAIFGKETGNQVKLVTM
CCCHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCHHHHHHEEEEEECCCCCEEEEEEC
PSSSSEQFSQYRLWLAAGNKDIDVYQTDVIWAPQLADQFIDLKEATKDVAGQFFPSIIAS
CCCCHHHHHHEEEEEEECCCCCEEEEECEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTVNGRLVALPLFTDAPALFYRKDLLEKYGKLPPKTWDEMAATAKEVQDKERQAGQKDLW
CCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
GYVFQGNSYEGLTCNALEWVKSSGGGQIVEPDGTISINNEKAAAALERAKGWIGTISPPG
EEEEECCCCCCEECHHHHHHHCCCCCEEECCCCEEEECCHHHHHHHHHHCCCEECCCCCC
VLAYQEEESRGVWQTGNAVFMRNWPYAYALGNGADSAVKGKFDVMTLPVAAAGDKPSSTL
EEEEECCCCCCCEECCCEEEEECCCEEEEECCCCCCCCCCCEEEEEEEEEECCCCCCCCC
GGWNLAVSKYSEKQEAAIALVKFLASKDVQKARAIELSNLPTLTDLYDDKDIAAAQPFMP
CCEEEEEHHHCHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCHHHHCCCCCCCCCCCCCC
NWKPIFQNAVPRPSATAKVKYNEVSSKFWTAVHNTLSGSGSAAENLELLEADLTTLKGDA
CCHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
W
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]