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The map label for this gene is ntaB [H]

Identifier: 86360928

GI number: 86360928

Start: 206683

End: 207675

Strand: Reverse

Name: ntaB [H]

Synonym: RHE_PF00197

Alternate gene names: 86360928

Gene position: 207675-206683 (Counterclockwise)

Preceding gene: 86360929

Following gene: 86360927

Centisome position: 32.32

GC content: 65.36

Gene sequence:

>993_bases
ATGGCGGAGACAGCGGCAAGGACGGATGCACCGATCGACCCGATCGCACTGCGCCGGGCCTTCGGAACCTTCGTGACAGG
TGTGACCGTCATCACCACGCGCGACGAGGACGGCAGCCCGCGCGGAATGACCGCGAATTCGTTTACCTCAGTGTCGCTCG
ATCCGCCGTTGCTTCTCGTCTGTGTCGCGAAGGCGGCCTCCAGCCATCAGGCCTTCACCAATGCCGGCTGCTTTGCGGTC
AATATCCTTCATGAAGGCCAAGTCGATATCTCTGGCACCTTCGCCTCGAAGTCTCCCGACAAGTTCCAGTCGGTCACCCA
CGATCACATCCATACCGGCGCGCCGGTTCTGACCGACAGTCTCACATGGTTCGACTGCACCACCCACAGCGCCGTCGATG
CTGGCGACCATACGGTTCTCATCGGCCAGGTCCGCGCCTTCGGCACCAGCCCGACCGCACCGCTCGGCTTCTGCCGCGGC
CGTTACGCCAGCGTCAAGGATCCGCTGCCGGCGGGCTGGCTTGCCTCGCACGGGGTGATCATCGGCTACTTGATCGAGGC
CGGCGACGGCCTGCTGCTGCGCTCGGACGGGAGGGGCGGCTGGGTGCTGCCATCGGCACGCCGCCGCAAGGCCGACAGCC
AGCTGGTGGTCGAGGGCGGCGATGCGCTGCGCCTCGTACCGGAAGAGACCTTCCTCTATTCCGTCTTCGACGTTGCCGAT
AGCGATCCCGGCTATCTCGTCTATCGCGCCCGGTTGGCAGGCGAGGGGGAAGAGGTCAACCTGCCTGCCGATCTCCGCTT
CTTCGGCATCGACGATCTGCCCTACGAGGCGATCGCCACCCATGAACTGCGCTCCATGTTGCGCCGCTATGTGCGCGAGC
GGCAGGCGGGCCGCTTCGGCATCTACATGGATTCCGACGACGGCGGCCGCGTCGCGATGATCGACGGCGAAGCCCGTGCC
TGGATCCAAGCCTCCCAAGACCAAGGACAGTGA

Upstream 100 bases:

>100_bases
ATCGAGGCAGAAGTTTTCCACCCCATCCTACGATGCCAGGGAAGAACGGCCGAAGCGGTAGCGATAGCCTCCTTTCAACA
AGCAGAGGAGGCTCGAAGGC

Downstream 100 bases:

>100_bases
CATGAAGACGACAGTCAGCTCCATCGAAGGTTCGCGACAGGGCCTGTTCATCAACGGCGAATTCGTGGCGCCGAAGGCCG
GCAACTACATTGCCAGCTAC

Product: nitrilotriacetate monooxygenase component B protein

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MAETAARTDAPIDPIALRRAFGTFVTGVTVITTRDEDGSPRGMTANSFTSVSLDPPLLLVCVAKAASSHQAFTNAGCFAV
NILHEGQVDISGTFASKSPDKFQSVTHDHIHTGAPVLTDSLTWFDCTTHSAVDAGDHTVLIGQVRAFGTSPTAPLGFCRG
RYASVKDPLPAGWLASHGVIIGYLIEAGDGLLLRSDGRGGWVLPSARRRKADSQLVVEGGDALRLVPEETFLYSVFDVAD
SDPGYLVYRARLAGEGEEVNLPADLRFFGIDDLPYEAIATHELRSMLRRYVRERQAGRFGIYMDSDDGGRVAMIDGEARA
WIQASQDQGQ

Sequences:

>Translated_330_residues
MAETAARTDAPIDPIALRRAFGTFVTGVTVITTRDEDGSPRGMTANSFTSVSLDPPLLLVCVAKAASSHQAFTNAGCFAV
NILHEGQVDISGTFASKSPDKFQSVTHDHIHTGAPVLTDSLTWFDCTTHSAVDAGDHTVLIGQVRAFGTSPTAPLGFCRG
RYASVKDPLPAGWLASHGVIIGYLIEAGDGLLLRSDGRGGWVLPSARRRKADSQLVVEGGDALRLVPEETFLYSVFDVAD
SDPGYLVYRARLAGEGEEVNLPADLRFFGIDDLPYEAIATHELRSMLRRYVRERQAGRFGIYMDSDDGGRVAMIDGEARA
WIQASQDQGQ
>Mature_329_residues
AETAARTDAPIDPIALRRAFGTFVTGVTVITTRDEDGSPRGMTANSFTSVSLDPPLLLVCVAKAASSHQAFTNAGCFAVN
ILHEGQVDISGTFASKSPDKFQSVTHDHIHTGAPVLTDSLTWFDCTTHSAVDAGDHTVLIGQVRAFGTSPTAPLGFCRGR
YASVKDPLPAGWLASHGVIIGYLIEAGDGLLLRSDGRGGWVLPSARRRKADSQLVVEGGDALRLVPEETFLYSVFDVADS
DPGYLVYRARLAGEGEEVNLPADLRFFGIDDLPYEAIATHELRSMLRRYVRERQAGRFGIYMDSDDGGRVAMIDGEARAW
IQASQDQGQ

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=159, Percent_Identity=32.0754716981132, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 35530; Mature: 35399

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETAARTDAPIDPIALRRAFGTFVTGVTVITTRDEDGSPRGMTANSFTSVSLDPPLLLV
CCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEECCCCCEEEE
CVAKAASSHQAFTNAGCFAVNILHEGQVDISGTFASKSPDKFQSVTHDHIHTGAPVLTDS
EEHHHCCCCCHHCCCCEEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEECC
LTWFDCTTHSAVDAGDHTVLIGQVRAFGTSPTAPLGFCRGRYASVKDPLPAGWLASHGVI
CEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCHHHHCCEE
IGYLIEAGDGLLLRSDGRGGWVLPSARRRKADSQLVVEGGDALRLVPEETFLYSVFDVAD
EEEEEECCCEEEEEECCCCCEECCCHHHCCCCCEEEEECCCEEEEECCHHEEEEEEECCC
SDPGYLVYRARLAGEGEEVNLPADLRFFGIDDLPYEAIATHELRSMLRRYVRERQAGRFG
CCCCEEEEEEEECCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
IYMDSDDGGRVAMIDGEARAWIQASQDQGQ
EEEECCCCCEEEEEECCCCEEEEECCCCCC
>Mature Secondary Structure 
AETAARTDAPIDPIALRRAFGTFVTGVTVITTRDEDGSPRGMTANSFTSVSLDPPLLLV
CCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCEEEECCCCCEEEE
CVAKAASSHQAFTNAGCFAVNILHEGQVDISGTFASKSPDKFQSVTHDHIHTGAPVLTDS
EEHHHCCCCCHHCCCCEEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEEECC
LTWFDCTTHSAVDAGDHTVLIGQVRAFGTSPTAPLGFCRGRYASVKDPLPAGWLASHGVI
CEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHCCCCCCCCCCCCCCHHHHCCEE
IGYLIEAGDGLLLRSDGRGGWVLPSARRRKADSQLVVEGGDALRLVPEETFLYSVFDVAD
EEEEEECCCEEEEEECCCCCEECCCHHHCCCCCEEEEECCCEEEEECCHHEEEEEEECCC
SDPGYLVYRARLAGEGEEVNLPADLRFFGIDDLPYEAIATHELRSMLRRYVRERQAGRFG
CCCCEEEEEEEECCCCCEECCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEE
IYMDSDDGGRVAMIDGEARAWIQASQDQGQ
EEEECCCCCEEEEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]