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The map label for this gene is peb1A [H]

Identifier: 86360898

GI number: 86360898

Start: 175037

End: 175876

Strand: Reverse

Name: peb1A [H]

Synonym: RHE_PF00167

Alternate gene names: 86360898

Gene position: 175876-175037 (Counterclockwise)

Preceding gene: 86360899

Following gene: 86360897

Centisome position: 27.37

GC content: 60.6

Gene sequence:

>840_bases
ATGACCATTTCCTTTCGCACCTGCGCGATGTCGCTGGCCGCCGCCGCGCTCATGTCCACGCCTGCACTGGCTGACGGCAG
CAAGCTCGACGAAGTGCTGGCCCGCGGCCATCTCGTGCTCGGCACGGGCAGCACCAATGCGCCCTGGCACTTCAAGAGCG
CTGACGACAAGCTTCAGGGCTTCGATGTCGACATGGGCCACATCGTCGCCAAGGCCCTGTTCGGCGACCCCGATAAGATC
GAATATGTCAACCAGTCCTCGGACGCTCGCATCCCGAACATCACCACTGACAAGGTCGACATCACCTGCCAGTTCATGAC
GGTTACGGGCGAACGCGCCCAGCAGGTGGCCTTTACTATTCCATATTATCGCGAGGGTGTCGGCCTGATGCTCAAGGGGG
ACGGAAAATATGCCGATTATGCCGCCCTGAAGGCTGCCGGCTCCTCGGTCACCATCTCGGTTCTCCAGAACGTCTATGCC
GAATCTATGGTGCACGCGGCATTGCCGGAGGCGACCGTCGACCAGTACGAATCCGTCGACCTGATCTATCAGGCGCTGGA
GTCGGGGCGTGCCGATGCCGCTGCCACGGATCAGTCGTCGCTCGCCTGGTACATGACGCAGAACCCGGGCCGCTACAAGG
ATGCCGGCTACGGCTGGAATCCACAGACCTATGCCTGCGCCGTCAAGCGCGGTGATCAGGACTGGCTGAACTTCGTCAAT
ACCGCTCTTCACGAAGCGATGACCGGTGTCGAGTTCGACTTCTATGCCAAATCCTTCAAGACCTGGTTCGGCAAGGATCT
GGCGCCGCCGCAGATCGGCTTCCCAGTCGAATACAAGTGA

Upstream 100 bases:

>100_bases
CATTGACAATTCACCGCCAATGGCACTTGATGCGTTATTCCAGTAAAACAGACATGCGTCCATTATATTGGAATAAAGGA
ACCATCCATAGGGGAACGCC

Downstream 100 bases:

>100_bases
CCGCGATGCGGGGCGGGAGCTTGCTTCCCGCTCCGCTGGATTAGGATGATCTTGGTCCAGGTCGGGCTAAGATCTAAGTC
CACTTCTGACTCGAATGGTT

Product: amino acid ABC transporter substrate-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: CBF1; PEB1 [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTISFRTCAMSLAAAALMSTPALADGSKLDEVLARGHLVLGTGSTNAPWHFKSADDKLQGFDVDMGHIVAKALFGDPDKI
EYVNQSSDARIPNITTDKVDITCQFMTVTGERAQQVAFTIPYYREGVGLMLKGDGKYADYAALKAAGSSVTISVLQNVYA
ESMVHAALPEATVDQYESVDLIYQALESGRADAAATDQSSLAWYMTQNPGRYKDAGYGWNPQTYACAVKRGDQDWLNFVN
TALHEAMTGVEFDFYAKSFKTWFGKDLAPPQIGFPVEYK

Sequences:

>Translated_279_residues
MTISFRTCAMSLAAAALMSTPALADGSKLDEVLARGHLVLGTGSTNAPWHFKSADDKLQGFDVDMGHIVAKALFGDPDKI
EYVNQSSDARIPNITTDKVDITCQFMTVTGERAQQVAFTIPYYREGVGLMLKGDGKYADYAALKAAGSSVTISVLQNVYA
ESMVHAALPEATVDQYESVDLIYQALESGRADAAATDQSSLAWYMTQNPGRYKDAGYGWNPQTYACAVKRGDQDWLNFVN
TALHEAMTGVEFDFYAKSFKTWFGKDLAPPQIGFPVEYK
>Mature_278_residues
TISFRTCAMSLAAAALMSTPALADGSKLDEVLARGHLVLGTGSTNAPWHFKSADDKLQGFDVDMGHIVAKALFGDPDKIE
YVNQSSDARIPNITTDKVDITCQFMTVTGERAQQVAFTIPYYREGVGLMLKGDGKYADYAALKAAGSSVTISVLQNVYAE
SMVHAALPEATVDQYESVDLIYQALESGRADAAATDQSSLAWYMTQNPGRYKDAGYGWNPQTYACAVKRGDQDWLNFVNT
ALHEAMTGVEFDFYAKSFKTWFGKDLAPPQIGFPVEYK

Specific function: Common antigen and a major cell adherence molecule. Most probably involved, with PEB1C, in a binding-protein-dependent transport system for an amino acid. May be involved in binding to intestinal cells [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell surface [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=234, Percent_Identity=29.0598290598291, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788228, Length=260, Percent_Identity=26.1538461538462, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1786876, Length=262, Percent_Identity=24.8091603053435, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787085, Length=208, Percent_Identity=25, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787088, Length=232, Percent_Identity=25.8620689655172, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788648, Length=238, Percent_Identity=25.6302521008403, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788649, Length=239, Percent_Identity=25.9414225941423, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 30454; Mature: 30323

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISFRTCAMSLAAAALMSTPALADGSKLDEVLARGHLVLGTGSTNAPWHFKSADDKLQG
CEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCC
FDVDMGHIVAKALFGDPDKIEYVNQSSDARIPNITTDKVDITCQFMTVTGERAQQVAFTI
CCCCHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEC
PYYREGVGLMLKGDGKYADYAALKAAGSSVTISVLQNVYAESMVHAALPEATVDQYESVD
CHHHCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LIYQALESGRADAAATDQSSLAWYMTQNPGRYKDAGYGWNPQTYACAVKRGDQDWLNFVN
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHH
TALHEAMTGVEFDFYAKSFKTWFGKDLAPPQIGFPVEYK
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TISFRTCAMSLAAAALMSTPALADGSKLDEVLARGHLVLGTGSTNAPWHFKSADDKLQG
EEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCCCCEEECCCCCCCCC
FDVDMGHIVAKALFGDPDKIEYVNQSSDARIPNITTDKVDITCQFMTVTGERAQQVAFTI
CCCCHHHHHHHHHCCCCHHEEEECCCCCCCCCCCCCCCEEEEEEEEEECCCCCCEEEEEC
PYYREGVGLMLKGDGKYADYAALKAAGSSVTISVLQNVYAESMVHAALPEATVDQYESVD
CHHHCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LIYQALESGRADAAATDQSSLAWYMTQNPGRYKDAGYGWNPQTYACAVKRGDQDWLNFVN
HHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHH
TALHEAMTGVEFDFYAKSFKTWFGKDLAPPQIGFPVEYK
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10688204; 1885571 [H]