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The map label for this gene is gntR [H]

Identifier: 86360820

GI number: 86360820

Start: 87772

End: 88719

Strand: Reverse

Name: gntR [H]

Synonym: RHE_PF00087

Alternate gene names: 86360820

Gene position: 88719-87772 (Counterclockwise)

Preceding gene: 86360837

Following gene: 86360818

Centisome position: 13.81

GC content: 60.65

Gene sequence:

>948_bases
ATGACCGCATCGCGCGCCCTTCGCGGGGCCGCCGATGTTTCCAAGGAGACGCGCGAAAAAGTGCTCCAGCAGGCCGAGCG
GCTAAACTATCTCGGCAACCGGTTGGCGCTCTCGCTGTCGTCGCAGCGCACCAACCTCGTCGCTGTCGTGGTCCCCAGCA
TGTCCAACATCGTCTTTCCCGAAATGTTGGCGGGGATTTCTGCCGGGCTGCGCGGATCCGGAATGCAGGCAGTCTTCGGC
ATCTCCGACTATGATATGGCGAAGGAACGCGAGATCATTCGCGACATGCTCTCCTGGAGGCCTGCCGCCATTATCGTCAC
AGGTCTCGATCAGCCCGCAGAAACTGTCAGAATGCTGCAGAATGCAGCCATCCCCGTCATTCAGCTCATGGACCTAGACG
GCACGCCGATCGATTTCAACGTCGGCCTTTCGCATGGCAAGGCTGGAGAAGAGATGGCAAAAGCGCTGTTGGCCGCCGGC
CGACGGCGGTTCGGCTATATCGGCAGCGCGATCGACAGGGATTTGCGCGCCGGCAAGCGAAAAGCCGGCTTCGAGAAGGT
GCTGCGCGAGAGCGGCCTCTCTTTCGTGGATGAGCGGTTCAACGCCGCCTATTCCTCGGTGGCGCACGGCAAGCGGCTAT
CGCTGTCGATGCTTGCGGCGACCAGGGACCTCGACTGCATCTATTATTCCAACGATGACATGGCGACGGGCGGCGCCTTT
GCCTGTCTGGAGCTCGGCATTTCCAGCCCCGCGGAGATCCTGATCGCCGGATTCAATGGGCTCGATGTCGCCAGCGCCCT
CCCCTTCAGGATCGCAACCTCGATTTCACCACGGCGTCAGATGGGCGAAGTCGCCGCCGCGCTTCTGCTCGCTGCGAGTA
GCGGTGAAGGAAACGAAATATCCGAAAAAATTATTGCCTTCACTCCGGAGATAACCGGCGTGGATTGA

Upstream 100 bases:

>100_bases
GAGTCTGACGTGCCCGCTGGCGAGGCAGTTCGACGATGGGGTCGGTGCGATGCGTAAATCCACTTTAGAAGAGGTTGCCG
CTGCGGCAGGCGTCAGCAAG

Downstream 100 bases:

>100_bases
GGTCTGAATGCCTCGTGCTCGGCTCCAGCCCGCTGGCCGCGTCGGGTTGGCGATTCGCAGCCTCCGTGTGCGCAAAAGCT
CCAGGCGGTTGAGCGCTCCT

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTASRALRGAADVSKETREKVLQQAERLNYLGNRLALSLSSQRTNLVAVVVPSMSNIVFPEMLAGISAGLRGSGMQAVFG
ISDYDMAKEREIIRDMLSWRPAAIIVTGLDQPAETVRMLQNAAIPVIQLMDLDGTPIDFNVGLSHGKAGEEMAKALLAAG
RRRFGYIGSAIDRDLRAGKRKAGFEKVLRESGLSFVDERFNAAYSSVAHGKRLSLSMLAATRDLDCIYYSNDDMATGGAF
ACLELGISSPAEILIAGFNGLDVASALPFRIATSISPRRQMGEVAAALLLAASSGEGNEISEKIIAFTPEITGVD

Sequences:

>Translated_315_residues
MTASRALRGAADVSKETREKVLQQAERLNYLGNRLALSLSSQRTNLVAVVVPSMSNIVFPEMLAGISAGLRGSGMQAVFG
ISDYDMAKEREIIRDMLSWRPAAIIVTGLDQPAETVRMLQNAAIPVIQLMDLDGTPIDFNVGLSHGKAGEEMAKALLAAG
RRRFGYIGSAIDRDLRAGKRKAGFEKVLRESGLSFVDERFNAAYSSVAHGKRLSLSMLAATRDLDCIYYSNDDMATGGAF
ACLELGISSPAEILIAGFNGLDVASALPFRIATSISPRRQMGEVAAALLLAASSGEGNEISEKIIAFTPEITGVD
>Mature_314_residues
TASRALRGAADVSKETREKVLQQAERLNYLGNRLALSLSSQRTNLVAVVVPSMSNIVFPEMLAGISAGLRGSGMQAVFGI
SDYDMAKEREIIRDMLSWRPAAIIVTGLDQPAETVRMLQNAAIPVIQLMDLDGTPIDFNVGLSHGKAGEEMAKALLAAGR
RRFGYIGSAIDRDLRAGKRKAGFEKVLRESGLSFVDERFNAAYSSVAHGKRLSLSMLAATRDLDCIYYSNDDMATGGAFA
CLELGISSPAEILIAGFNGLDVASALPFRIATSISPRRQMGEVAAALLLAASSGEGNEISEKIIAFTPEITGVD

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994940, Length=299, Percent_Identity=33.7792642140468, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1790715, Length=293, Percent_Identity=32.7645051194539, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788474, Length=299, Percent_Identity=26.0869565217391, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1787948, Length=292, Percent_Identity=23.6301369863014, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789068, Length=299, Percent_Identity=23.4113712374582, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790369, Length=296, Percent_Identity=21.9594594594595, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786540, Length=304, Percent_Identity=24.3421052631579, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789202, Length=279, Percent_Identity=25.089605734767, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33647; Mature: 33516

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASRALRGAADVSKETREKVLQQAERLNYLGNRLALSLSSQRTNLVAVVVPSMSNIVFP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCCHHHH
EMLAGISAGLRGSGMQAVFGISDYDMAKEREIIRDMLSWRPAAIIVTGLDQPAETVRMLQ
HHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
NAAIPVIQLMDLDGTPIDFNVGLSHGKAGEEMAKALLAAGRRRFGYIGSAIDRDLRAGKR
HCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHH
KAGFEKVLRESGLSFVDERFNAAYSSVAHGKRLSLSMLAATRDLDCIYYSNDDMATGGAF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCCCCHH
ACLELGISSPAEILIAGFNGLDVASALPFRIATSISPRRQMGEVAAALLLAASSGEGNEI
HHHHHCCCCCCEEEEECCCCCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHH
SEKIIAFTPEITGVD
HHHHEEECCCCCCCC
>Mature Secondary Structure 
TASRALRGAADVSKETREKVLQQAERLNYLGNRLALSLSSQRTNLVAVVVPSMSNIVFP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCCHHHH
EMLAGISAGLRGSGMQAVFGISDYDMAKEREIIRDMLSWRPAAIIVTGLDQPAETVRMLQ
HHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
NAAIPVIQLMDLDGTPIDFNVGLSHGKAGEEMAKALLAAGRRRFGYIGSAIDRDLRAGKR
HCCCCEEEEEECCCCCEEEECCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCHH
KAGFEKVLRESGLSFVDERFNAAYSSVAHGKRLSLSMLAATRDLDCIYYSNDDMATGGAF
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCCCCHH
ACLELGISSPAEILIAGFNGLDVASALPFRIATSISPRRQMGEVAAALLLAASSGEGNEI
HHHHHCCCCCCEEEEECCCCCCHHHHCCHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHH
SEKIIAFTPEITGVD
HHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]