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The map label for this gene is nadB [H]

Identifier: 85859993

GI number: 85859993

Start: 2213430

End: 2215142

Strand: Direct

Name: nadB [H]

Synonym: SYN_00729

Alternate gene names: 85859993

Gene position: 2213430-2215142 (Clockwise)

Preceding gene: 85859990

Following gene: 85859996

Centisome position: 69.62

GC content: 44.95

Gene sequence:

>1713_bases
GTGTCGATGCTGCCGCAATTGATCAACATGGATACACAGCATACAGAGATCGTTGTTACAGATATCCTGATCATTGGGAG
TGGCATCGCGGGACTCAGAGCGGCTCTCGAAGCAAGTAAATGCGGGAAAAAAGTAATTATACTGACTAAAAGCAGAATCA
CCGATTCGAATACCTATTACGCCCAAGGCGGAATCACAGGTTTAGACCCTGAACGTGTAGCCAGAGGGAAAGACAGTTAT
GAATCGCTCATACAGAATACACTGGCAGCAGGTGATGGCCTGTGCAACAAGCAAGTTGTGGATTTTTTTGCACATCATTC
TTACGAACCAATAAAGTTTTTGATTCAGCAGAATGTTCCTTTTTCAAAGGATGCTGACGGCTGGATACTGCACCAGGAAG
GCGGCCATGAGCATGAAAGAATATACTGTGTCGCGGATTATACGGGCAAAGCGATTGAAGAAACACTGGCAGGCAAGGTG
CTGCAGGACAAAAACATAAAAGTATATGAACATTTTCTTACCTTCACATTAATAACCAGGCGTAAACTCAGGAAAAATCC
AAACCTTGACGACAGGTGTCTTGGTGTTTACGCGATAGACCGCCTGAATGAAACAGTGGCAGCATTCAAGGCAAACGCCG
TAATACTGGCAACGGGTGGAGCTGGAAGAGTATTTCAGTACACAAGCAACCCGGACAATGCTACCGGCGACGGGATTGCA
ATGGCATATTACGTTGGGGCAAAAATCGCTAATATGGAATTCTTTCAGTTTCATCCGACGGTATTATACGAACCCTTTGG
AATGCAGCCTAATGAACGGAGATTTCTGCTGACCGAAGCATTACGTGGAGTCAGCGTGGGCGGAATACTGACCACCAGCC
CGGATTCTACGGAAGATTTTGTATTAGCGTATCATCCTGACGGATCGCACGCGACTCGTGATAAAGTATCGCAGGCTGTC
GATGCTGAAATGAAAAGATCAGGGTTAAAACATGTTTATTTGAATGTCACCACGAGAATTTCAGGAAAAACTCCCGAATA
TATCAAGGAACATTTTCCCCAGATATACGCTCACTGCCTCAACAAAGGCATAGATATAACCGTGCAGCCAATCCCTGTCG
TTCCGGCTGCACATTATACTTGTGGAGGAGTACTGGTTGATATCCGCGGGAGAACAAGCATTCCCGGATTGTTTGCAATA
GGCGAAGTATCATGTACCGGGCTGATGGGAGCGAATCGTCTGGCGTCTAATTCGTTGGCTGAAGCGGCATTATTTGGAAA
AGTTGCAGCAGATGAAGCGATAAAATTTTCTGAAAACAAAAATACAGAGGATGTGCCTGCCTGGTATACCACCGGAATCA
TCAGCCATGCAGACGTCGCGACAGTAAACCAGATATGGGATGTGGCCAGAACGACGATGATGAGTTTATGCGGAATCGAC
AGAACTGAAGAGAGATTAAGAGCAGCCGTCGGCATATTGGATAGTTTATATGACACAATAAACAAAATATACTGGGGGTA
TTATCCCAACGATCAAATATTTGAAACCAGAAACCTGGTGCAGGCAGCAAAATTAATCGCCAAAGCGGCATTATTCAGAA
AGGAAAGTCGTGGAGGGCATTACAGGTCAGATTATCCACAGAAAAAACCTGAATACGAATCCATGACATTAATACAGAAC
GGAACAGAGATGAAACTAGTGCCCGTAGAATAA

Upstream 100 bases:

>100_bases
ATGACAGTGCCGTTAGAATAAGCGACCCGGAAAATAAACATCAATTTATCTACAATATCGGAGGAAAACGGTCGCATCGA
CACAGGATGCTGGCACATGC

Downstream 100 bases:

>100_bases
TCAATCAAATTTTAATGTCCTGAACGGTCCTGAACAAGACTTGAGTTGACAGTAGCCCGATTCTTCCCGCAATGTTTATT
TATACTTTTCATCCCTGTTC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MSMLPQLINMDTQHTEIVVTDILIIGSGIAGLRAALEASKCGKKVIILTKSRITDSNTYYAQGGITGLDPERVARGKDSY
ESLIQNTLAAGDGLCNKQVVDFFAHHSYEPIKFLIQQNVPFSKDADGWILHQEGGHEHERIYCVADYTGKAIEETLAGKV
LQDKNIKVYEHFLTFTLITRRKLRKNPNLDDRCLGVYAIDRLNETVAAFKANAVILATGGAGRVFQYTSNPDNATGDGIA
MAYYVGAKIANMEFFQFHPTVLYEPFGMQPNERRFLLTEALRGVSVGGILTTSPDSTEDFVLAYHPDGSHATRDKVSQAV
DAEMKRSGLKHVYLNVTTRISGKTPEYIKEHFPQIYAHCLNKGIDITVQPIPVVPAAHYTCGGVLVDIRGRTSIPGLFAI
GEVSCTGLMGANRLASNSLAEAALFGKVAADEAIKFSENKNTEDVPAWYTTGIISHADVATVNQIWDVARTTMMSLCGID
RTEERLRAAVGILDSLYDTINKIYWGYYPNDQIFETRNLVQAAKLIAKAALFRKESRGGHYRSDYPQKKPEYESMTLIQN
GTEMKLVPVE

Sequences:

>Translated_570_residues
MSMLPQLINMDTQHTEIVVTDILIIGSGIAGLRAALEASKCGKKVIILTKSRITDSNTYYAQGGITGLDPERVARGKDSY
ESLIQNTLAAGDGLCNKQVVDFFAHHSYEPIKFLIQQNVPFSKDADGWILHQEGGHEHERIYCVADYTGKAIEETLAGKV
LQDKNIKVYEHFLTFTLITRRKLRKNPNLDDRCLGVYAIDRLNETVAAFKANAVILATGGAGRVFQYTSNPDNATGDGIA
MAYYVGAKIANMEFFQFHPTVLYEPFGMQPNERRFLLTEALRGVSVGGILTTSPDSTEDFVLAYHPDGSHATRDKVSQAV
DAEMKRSGLKHVYLNVTTRISGKTPEYIKEHFPQIYAHCLNKGIDITVQPIPVVPAAHYTCGGVLVDIRGRTSIPGLFAI
GEVSCTGLMGANRLASNSLAEAALFGKVAADEAIKFSENKNTEDVPAWYTTGIISHADVATVNQIWDVARTTMMSLCGID
RTEERLRAAVGILDSLYDTINKIYWGYYPNDQIFETRNLVQAAKLIAKAALFRKESRGGHYRSDYPQKKPEYESMTLIQN
GTEMKLVPVE
>Mature_569_residues
SMLPQLINMDTQHTEIVVTDILIIGSGIAGLRAALEASKCGKKVIILTKSRITDSNTYYAQGGITGLDPERVARGKDSYE
SLIQNTLAAGDGLCNKQVVDFFAHHSYEPIKFLIQQNVPFSKDADGWILHQEGGHEHERIYCVADYTGKAIEETLAGKVL
QDKNIKVYEHFLTFTLITRRKLRKNPNLDDRCLGVYAIDRLNETVAAFKANAVILATGGAGRVFQYTSNPDNATGDGIAM
AYYVGAKIANMEFFQFHPTVLYEPFGMQPNERRFLLTEALRGVSVGGILTTSPDSTEDFVLAYHPDGSHATRDKVSQAVD
AEMKRSGLKHVYLNVTTRISGKTPEYIKEHFPQIYAHCLNKGIDITVQPIPVVPAAHYTCGGVLVDIRGRTSIPGLFAIG
EVSCTGLMGANRLASNSLAEAALFGKVAADEAIKFSENKNTEDVPAWYTTGIISHADVATVNQIWDVARTTMMSLCGIDR
TEERLRAAVGILDSLYDTINKIYWGYYPNDQIFETRNLVQAAKLIAKAALFRKESRGGHYRSDYPQKKPEYESMTLIQNG
TEMKLVPVE

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=570, Percent_Identity=28.4210526315789, Blast_Score=176, Evalue=5e-44,
Organism=Escherichia coli, GI1788928, Length=533, Percent_Identity=42.5891181988743, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1790597, Length=537, Percent_Identity=32.9608938547486, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1786942, Length=611, Percent_Identity=29.6235679214403, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17550100, Length=570, Percent_Identity=28.7719298245614, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17505833, Length=569, Percent_Identity=28.9982425307557, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI71986328, Length=268, Percent_Identity=28.7313432835821, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322701, Length=580, Percent_Identity=30.8620689655172, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6322416, Length=559, Percent_Identity=27.906976744186, Blast_Score=183, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6322511, Length=477, Percent_Identity=27.0440251572327, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17137288, Length=564, Percent_Identity=28.5460992907801, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24655642, Length=564, Percent_Identity=28.5460992907801, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24655647, Length=564, Percent_Identity=28.5460992907801, Blast_Score=174, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24663005, Length=555, Percent_Identity=26.6666666666667, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 62994; Mature: 62862

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMLPQLINMDTQHTEIVVTDILIIGSGIAGLRAALEASKCGKKVIILTKSRITDSNTYY
CCCCCHHHCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEE
AQGGITGLDPERVARGKDSYESLIQNTLAAGDGLCNKQVVDFFAHHSYEPIKFLIQQNVP
ECCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCC
FSKDADGWILHQEGGHEHERIYCVADYTGKAIEETLAGKVLQDKNIKVYEHFLTFTLITR
CCCCCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHH
RKLRKNPNLDDRCLGVYAIDRLNETVAAFKANAVILATGGAGRVFQYTSNPDNATGDGIA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCEE
MAYYVGAKIANMEFFQFHPTVLYEPFGMQPNERRFLLTEALRGVSVGGILTTSPDSTEDF
EEEEECCEEECEEEEEECCEEEECCCCCCCCCCEEHHHHHHCCCEECCEEECCCCCCCCE
VLAYHPDGSHATRDKVSQAVDAEMKRSGLKHVYLNVTTRISGKTPEYIKEHFPQIYAHCL
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHH
NKGIDITVQPIPVVPAAHYTCGGVLVDIRGRTSIPGLFAIGEVSCTGLMGANRLASNSLA
CCCCEEEEECCCCCCCCCEECCEEEEEECCCCCCCCEEEECCEEEECCCCCCHHHHHHHH
EAALFGKVAADEAIKFSENKNTEDVPAWYTTGIISHADVATVNQIWDVARTTMMSLCGID
HHHHHHHHHHHHHEEECCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
RTEERLRAAVGILDSLYDTINKIYWGYYPNDQIFETRNLVQAAKLIAKAALFRKESRGGH
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YRSDYPQKKPEYESMTLIQNGTEMKLVPVE
CCCCCCCCCCCCCCEEEEECCCEEEEEECC
>Mature Secondary Structure 
SMLPQLINMDTQHTEIVVTDILIIGSGIAGLRAALEASKCGKKVIILTKSRITDSNTYY
CCCCHHHCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEE
AQGGITGLDPERVARGKDSYESLIQNTLAAGDGLCNKQVVDFFAHHSYEPIKFLIQQNVP
ECCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCC
FSKDADGWILHQEGGHEHERIYCVADYTGKAIEETLAGKVLQDKNIKVYEHFLTFTLITR
CCCCCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHH
RKLRKNPNLDDRCLGVYAIDRLNETVAAFKANAVILATGGAGRVFQYTSNPDNATGDGIA
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCEEEEECCCCCCCCCCEE
MAYYVGAKIANMEFFQFHPTVLYEPFGMQPNERRFLLTEALRGVSVGGILTTSPDSTEDF
EEEEECCEEECEEEEEECCEEEECCCCCCCCCCEEHHHHHHCCCEECCEEECCCCCCCCE
VLAYHPDGSHATRDKVSQAVDAEMKRSGLKHVYLNVTTRISGKTPEYIKEHFPQIYAHCL
EEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHH
NKGIDITVQPIPVVPAAHYTCGGVLVDIRGRTSIPGLFAIGEVSCTGLMGANRLASNSLA
CCCCEEEEECCCCCCCCCEECCEEEEEECCCCCCCCEEEECCEEEECCCCCCHHHHHHHH
EAALFGKVAADEAIKFSENKNTEDVPAWYTTGIISHADVATVNQIWDVARTTMMSLCGID
HHHHHHHHHHHHHEEECCCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
RTEERLRAAVGILDSLYDTINKIYWGYYPNDQIFETRNLVQAAKLIAKAALFRKESRGGH
HHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
YRSDYPQKKPEYESMTLIQNGTEMKLVPVE
CCCCCCCCCCCCCCEEEEECCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]