Click here to switch to the map view.

The map label for this gene is hybA [C]

Identifier: 85859984

GI number: 85859984

Start: 2207036

End: 2208046

Strand: Direct

Name: hybA [C]

Synonym: SYN_00720

Alternate gene names: 85859984

Gene position: 2207036-2208046 (Clockwise)

Preceding gene: 85859983

Following gene: 85859985

Centisome position: 69.42

GC content: 53.02

Gene sequence:

>1011_bases
ATGGAAGCGGAAAAAATATCAAAGATCAGTCGTCGTTCCCTCCTGAAGGCATCTACTGGGGCATTGGCCATTCCGGGCTT
AGCATTCCCAGGAACGGTCCAGGCAGATGTAAAAGAACAACTGGCAACTCTGATTGATCTCAGTCGATGCAATGGTTGCG
TGGACCTTGCGAAACCGTCCTGCGTCACCGTTTGTAAAGAGATCAACAAAAACACCATTCCGGCGATTGCTGATCCCATT
CCCGTGCCATGGCCACGCAAGACAGTCGAGGACTGGTCCAGGAAAAGGGAGGTCAGCAACCGCTTGACACCCTATAATTT
CATTTATGTTCACAAGGCCGACATCGTCTGGAAAGGAAAGCCGCAAACCATTTATGTTCCAAGGAGGTGCATGCACTGCG
ACAACCCGGCATGCGCAACAATCTGTCCCTTTTCGGCAAATCATAAGCAAAGGAACGGAGCGGTGGTGATCGATCAGGAT
TTCTGTTTCGGTGGGGCCAAATGCAGGACGGTCTGTCCCTGGGAGATCCCCCAGCGGCAGTCCGGTGTAGGCATTTATCT
GCACGTTCTGCCTGCCCTGATGGGAAACGGTGTCATGTACAAATGCGACCTCTGCAATCAGCGATTAAAGGAGGGAAAGC
CACCCGGCTGTGTGGAGGCCTGTCCAAGGGAAGCCATGATCATCGGGCCCAGAAAAGAGATTTATGCAGTAGCGGAAGAA
CGGATTAAGGCCATGGATGGTTACATATACGGAAAGGAAGAAAACGGCGGGACGGCAACCCTGTATGTATCCCCGGTTCC
CTTTGAACTGATCAATGAAACCATGAAGAAAAAACCGGGCCAGCCCGACATGAAGCCAGGTGTCGAACGGCGAATGGCAG
GAACGGATCCCGCAGGGAAACTGGCGTTGATTGCGCCGGTACTGGGAATTGCTGCCAGCGCTGCAGCGGGCTTTAACTGG
CTGTCCCGGCGGAAGCAGAAGGTGCAGGAAGAGGAAAAAGACAATGGCTGA

Upstream 100 bases:

>100_bases
AATGTATTTCCGAAACCTCTCAGGGAAACGATGAGGCACTGGAAAATTTTAAGAAAGCTTTAGCCCGATATATCCGCATG
GCATAAAGAAGGAGTAAAAG

Downstream 100 bases:

>100_bases
AAAGATTTTGCAAGAAAAGGGTCTCGTTGTCCGTCATGGAAGCGTTGAATTAATCGAGCACTGGGGTATTGCCCTTTCCG
GACTGGTTCTACTGCTATCA

Product: exported Fe-S-cluster-containing protein

Products: NA

Alternate protein names: HMC operon ORF 2 [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MEAEKISKISRRSLLKASTGALAIPGLAFPGTVQADVKEQLATLIDLSRCNGCVDLAKPSCVTVCKEINKNTIPAIADPI
PVPWPRKTVEDWSRKREVSNRLTPYNFIYVHKADIVWKGKPQTIYVPRRCMHCDNPACATICPFSANHKQRNGAVVIDQD
FCFGGAKCRTVCPWEIPQRQSGVGIYLHVLPALMGNGVMYKCDLCNQRLKEGKPPGCVEACPREAMIIGPRKEIYAVAEE
RIKAMDGYIYGKEENGGTATLYVSPVPFELINETMKKKPGQPDMKPGVERRMAGTDPAGKLALIAPVLGIAASAAAGFNW
LSRRKQKVQEEEKDNG

Sequences:

>Translated_336_residues
MEAEKISKISRRSLLKASTGALAIPGLAFPGTVQADVKEQLATLIDLSRCNGCVDLAKPSCVTVCKEINKNTIPAIADPI
PVPWPRKTVEDWSRKREVSNRLTPYNFIYVHKADIVWKGKPQTIYVPRRCMHCDNPACATICPFSANHKQRNGAVVIDQD
FCFGGAKCRTVCPWEIPQRQSGVGIYLHVLPALMGNGVMYKCDLCNQRLKEGKPPGCVEACPREAMIIGPRKEIYAVAEE
RIKAMDGYIYGKEENGGTATLYVSPVPFELINETMKKKPGQPDMKPGVERRMAGTDPAGKLALIAPVLGIAASAAAGFNW
LSRRKQKVQEEEKDNG
>Mature_336_residues
MEAEKISKISRRSLLKASTGALAIPGLAFPGTVQADVKEQLATLIDLSRCNGCVDLAKPSCVTVCKEINKNTIPAIADPI
PVPWPRKTVEDWSRKREVSNRLTPYNFIYVHKADIVWKGKPQTIYVPRRCMHCDNPACATICPFSANHKQRNGAVVIDQD
FCFGGAKCRTVCPWEIPQRQSGVGIYLHVLPALMGNGVMYKCDLCNQRLKEGKPPGCVEACPREAMIIGPRKEIYAVAEE
RIKAMDGYIYGKEENGGTATLYVSPVPFELINETMKKKPGQPDMKPGVERRMAGTDPAGKLALIAPVLGIAASAAAGFNW
LSRRKQKVQEEEKDNG

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789370, Length=305, Percent_Identity=30.8196721311475, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787872, Length=199, Percent_Identity=32.1608040201005, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787122, Length=195, Percent_Identity=31.2820512820513, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1787749, Length=308, Percent_Identity=25.6493506493506, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790326, Length=235, Percent_Identity=26.8085106382979, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787478, Length=117, Percent_Identity=35.8974358974359, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI226510944, Length=226, Percent_Identity=25.2212389380531, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787740, Length=99, Percent_Identity=36.3636363636364, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI2367345, Length=270, Percent_Identity=24.0740740740741, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 36939; Mature: 36939

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAEKISKISRRSLLKASTGALAIPGLAFPGTVQADVKEQLATLIDLSRCNGCVDLAKPS
CCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCH
CVTVCKEINKNTIPAIADPIPVPWPRKTVEDWSRKREVSNRLTPYNFIYVHKADIVWKGK
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCC
PQTIYVPRRCMHCDNPACATICPFSANHKQRNGAVVIDQDFCFGGAKCRTVCPWEIPQRQ
CCEEECCHHHHCCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCCCEECCCCCCCCC
SGVGIYLHVLPALMGNGVMYKCDLCNQRLKEGKPPGCVEACPREAMIIGPRKEIYAVAEE
CCCEEHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCHHHHCCCCCEEECCHHHHHHHHHH
RIKAMDGYIYGKEENGGTATLYVSPVPFELINETMKKKPGQPDMKPGVERRMAGTDPAGK
HHHHHCCEEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCC
LALIAPVLGIAASAAAGFNWLSRRKQKVQEEEKDNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEAEKISKISRRSLLKASTGALAIPGLAFPGTVQADVKEQLATLIDLSRCNGCVDLAKPS
CCHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCH
CVTVCKEINKNTIPAIADPIPVPWPRKTVEDWSRKREVSNRLTPYNFIYVHKADIVWKGK
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEEEEECCC
PQTIYVPRRCMHCDNPACATICPFSANHKQRNGAVVIDQDFCFGGAKCRTVCPWEIPQRQ
CCEEECCHHHHCCCCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCCCEECCCCCCCCC
SGVGIYLHVLPALMGNGVMYKCDLCNQRLKEGKPPGCVEACPREAMIIGPRKEIYAVAEE
CCCEEHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCHHHHCCCCCEEECCHHHHHHHHHH
RIKAMDGYIYGKEENGGTATLYVSPVPFELINETMKKKPGQPDMKPGVERRMAGTDPAGK
HHHHHCCEEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCC
LALIAPVLGIAASAAAGFNWLSRRKQKVQEEEKDNG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]