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The map label for this gene is tig

Identifier: 85859379

GI number: 85859379

Start: 1578705

End: 1580036

Strand: Direct

Name: tig

Synonym: SYN_02784

Alternate gene names: 85859379

Gene position: 1578705-1580036 (Clockwise)

Preceding gene: 85859377

Following gene: 85859380

Centisome position: 49.66

GC content: 41.82

Gene sequence:

>1332_bases
GTGGATGAAAATACTGTGGCGGTAAAGGTCGAAGAAATCAGCCCCGTAAAGAAGAAGCTTTTGTTCGATGTACCATGGGA
GGATGTAAAAAGGGAAAGAGACACCATATACCGTAATTTCAGCAGGACCGCCAAGATCAAGGGTTTTCGGAAGGGAAAAA
TTCCCCGGAAAATTCTGGAATTACATTATAAAAAGCATGCCGAGGAAGAAACGGTTTCGAGCCTGGTAAGCAGGCTATAT
TGGGATGCCCTGAAAGAACATGACATAAGAGCAATAAGTCAACCGGAGATTGAACAGCAGGGAATTGAAGAGGAAAAAGA
CTTTTCCTTTTCAGCAACTGTTGAAGTTGAACCGCAGATTGATCCGCAGGGCTATCTCGACATGACGCTCAGTAAAGAAG
AACAGACCGTAACAGAAGATGAGGTTGACGGTCGTCTCGAAGATATCCGTAAAATGTTCGGTACTCTGGAAGAAGTAACG
GAGGATCGAGAAATCAGAAAGGGAGATTTTGTCACCATCGATTTTGAAGGTTTTATTGATGGTCAGAATCCCGAAGAAAT
GAAGCGCAACGATTATTTTCTGGAAATCGGGTCAAATACCATGGTTCCCGGATTCGAAGACCAGCTTCTCGGAGAGAAAG
TCGGAACGATAAAGGAAATTAAAATCATATTTCCTGAAAATTACCAGATCAACAGCAATGTGGCTGGAAAGGACGCAACC
TTCAAGGTCACGATTAAAAACCTTAAGGAAAAGAAACTCCCTGAGCTGGATGAAAATTTTGTTAAAAATTTCGATAAGTA
CGAATCACTGGATGACTTGAAGGAAGATGTCCGCAAGTCCCTGTTTGAAGACCACAAGAGAAAAACCGAAGCGGACATGT
CCAAAAAGATTATGGATAAATTACTGGAGATGAATGACTTCGATGCGCCGTCGTCATACGTCGAACGACAGATCTTTTTT
ATGATGATGGAAGCACGGAAAAGGCTTATCACCACGGGAATGGATCCAGCGATGATCGATCAGGTCAGCGCTTCATGGCA
TGACAAATATAAAGATGAAGCTACACGGATCGTGAAATCGTCCCTTCTTCTAAAGAGTATTGCGCAGAAGGAATCGATTG
ATGTGACTGACGAGGAATTTGAGGAACGTTTGAGAGAAATTGCGCGACAATACTCGCAAGATTACGAAAAGATAAAGGAT
TCCTTTAATGAAGATATGAAAGAGAATGTAAGAAACGACATTCTGAATAAAAAGGTCTTTGACTTTATTGAATCAAAAGC
ATCCGTTTCAATGATCGAAAAAGAAAAGACCTCACCTGAGGAGGCAAACTGA

Upstream 100 bases:

>100_bases
GCCCGCTGTGGCGGATAGGGGTTCAAGTCCCCTCTCTCGCACCATACTTTGTGAATCATGAGCTTTAATTTCCAAATATC
ATTGCTAAGGAGTTAACATT

Downstream 100 bases:

>100_bases
TGAATCTCATTCCGATGGTGGTTCAGCAGGATGGGCGCGGTGAACGGGCATATGATATTTATTCGCGATTACTCAAGGAT
CGGATTATCTTTCTTGGTAC

Product: trigger factor, PPIase

Products: peptidylproline (omega=0)

Alternate protein names: TF

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MDENTVAVKVEEISPVKKKLLFDVPWEDVKRERDTIYRNFSRTAKIKGFRKGKIPRKILELHYKKHAEEETVSSLVSRLY
WDALKEHDIRAISQPEIEQQGIEEEKDFSFSATVEVEPQIDPQGYLDMTLSKEEQTVTEDEVDGRLEDIRKMFGTLEEVT
EDREIRKGDFVTIDFEGFIDGQNPEEMKRNDYFLEIGSNTMVPGFEDQLLGEKVGTIKEIKIIFPENYQINSNVAGKDAT
FKVTIKNLKEKKLPELDENFVKNFDKYESLDDLKEDVRKSLFEDHKRKTEADMSKKIMDKLLEMNDFDAPSSYVERQIFF
MMMEARKRLITTGMDPAMIDQVSASWHDKYKDEATRIVKSSLLLKSIAQKESIDVTDEEFEERLREIARQYSQDYEKIKD
SFNEDMKENVRNDILNKKVFDFIESKASVSMIEKEKTSPEEAN

Sequences:

>Translated_443_residues
MDENTVAVKVEEISPVKKKLLFDVPWEDVKRERDTIYRNFSRTAKIKGFRKGKIPRKILELHYKKHAEEETVSSLVSRLY
WDALKEHDIRAISQPEIEQQGIEEEKDFSFSATVEVEPQIDPQGYLDMTLSKEEQTVTEDEVDGRLEDIRKMFGTLEEVT
EDREIRKGDFVTIDFEGFIDGQNPEEMKRNDYFLEIGSNTMVPGFEDQLLGEKVGTIKEIKIIFPENYQINSNVAGKDAT
FKVTIKNLKEKKLPELDENFVKNFDKYESLDDLKEDVRKSLFEDHKRKTEADMSKKIMDKLLEMNDFDAPSSYVERQIFF
MMMEARKRLITTGMDPAMIDQVSASWHDKYKDEATRIVKSSLLLKSIAQKESIDVTDEEFEERLREIARQYSQDYEKIKD
SFNEDMKENVRNDILNKKVFDFIESKASVSMIEKEKTSPEEAN
>Mature_443_residues
MDENTVAVKVEEISPVKKKLLFDVPWEDVKRERDTIYRNFSRTAKIKGFRKGKIPRKILELHYKKHAEEETVSSLVSRLY
WDALKEHDIRAISQPEIEQQGIEEEKDFSFSATVEVEPQIDPQGYLDMTLSKEEQTVTEDEVDGRLEDIRKMFGTLEEVT
EDREIRKGDFVTIDFEGFIDGQNPEEMKRNDYFLEIGSNTMVPGFEDQLLGEKVGTIKEIKIIFPENYQINSNVAGKDAT
FKVTIKNLKEKKLPELDENFVKNFDKYESLDDLKEDVRKSLFEDHKRKTEADMSKKIMDKLLEMNDFDAPSSYVERQIFF
MMMEARKRLITTGMDPAMIDQVSASWHDKYKDEATRIVKSSLLLKSIAQKESIDVTDEEFEERLREIARQYSQDYEKIKD
SFNEDMKENVRNDILNKKVFDFIESKASVSMIEKEKTSPEEAN

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=438, Percent_Identity=28.7671232876712, Blast_Score=175, Evalue=6e-45,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_SYNAS (Q2LTK6)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461581.1
- ProteinModelPortal:   Q2LTK6
- STRING:   Q2LTK6
- GeneID:   3882833
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_02784
- NMPDR:   fig|56780.10.peg.1526
- eggNOG:   COG0544
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- PhylomeDB:   Q2LTK6
- BioCyc:   SACI56780:SYN_02784-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: 5.2.1.8

Molecular weight: Translated: 51905; Mature: 51905

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDENTVAVKVEEISPVKKKLLFDVPWEDVKRERDTIYRNFSRTAKIKGFRKGKIPRKILE
CCCCEEEEEEHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LHYKKHAEEETVSSLVSRLYWDALKEHDIRAISQPEIEQQGIEEEKDFSFSATVEVEPQI
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHCCCHHHCCCEEEEEEECCCC
DPQGYLDMTLSKEEQTVTEDEVDGRLEDIRKMFGTLEEVTEDREIRKGDFVTIDFEGFID
CCCCCEEEEECCCHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
GQNPEEMKRNDYFLEIGSNTMVPGFEDQLLGEKVGTIKEIKIIFPENYQINSNVAGKDAT
CCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCE
FKVTIKNLKEKKLPELDENFVKNFDKYESLDDLKEDVRKSLFEDHKRKTEADMSKKIMDK
EEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEMNDFDAPSSYVERQIFFMMMEARKRLITTGMDPAMIDQVSASWHDKYKDEATRIVKS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLLLKSIAQKESIDVTDEEFEERLREIARQYSQDYEKIKDSFNEDMKENVRNDILNKKVF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFIESKASVSMIEKEKTSPEEAN
HHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDENTVAVKVEEISPVKKKLLFDVPWEDVKRERDTIYRNFSRTAKIKGFRKGKIPRKILE
CCCCEEEEEEHHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LHYKKHAEEETVSSLVSRLYWDALKEHDIRAISQPEIEQQGIEEEKDFSFSATVEVEPQI
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCHHHHCCCHHHCCCEEEEEEECCCC
DPQGYLDMTLSKEEQTVTEDEVDGRLEDIRKMFGTLEEVTEDREIRKGDFVTIDFEGFID
CCCCCEEEEECCCHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
GQNPEEMKRNDYFLEIGSNTMVPGFEDQLLGEKVGTIKEIKIIFPENYQINSNVAGKDAT
CCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEEECCCCEEECCCCCCCCCE
FKVTIKNLKEKKLPELDENFVKNFDKYESLDDLKEDVRKSLFEDHKRKTEADMSKKIMDK
EEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEMNDFDAPSSYVERQIFFMMMEARKRLITTGMDPAMIDQVSASWHDKYKDEATRIVKS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SLLLKSIAQKESIDVTDEEFEERLREIARQYSQDYEKIKDSFNEDMKENVRNDILNKKVF
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFIESKASVSMIEKEKTSPEEAN
HHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (omega=180)

Specific reaction: peptidylproline (omega=180) = peptidylproline (omega=0)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA