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The map label for this gene is miaA

Identifier: 85859365

GI number: 85859365

Start: 1562883

End: 1563800

Strand: Direct

Name: miaA

Synonym: SYN_01969

Alternate gene names: 85859365

Gene position: 1562883-1563800 (Clockwise)

Preceding gene: 85859357

Following gene: 85859366

Centisome position: 49.16

GC content: 52.51

Gene sequence:

>918_bases
ATGATGAAGAACCTCGTTGTTATCCTCGGACCGACTGCATCGGGTAAGACCAGGCTGGCTGTCCGGCTGGCCCGGGATTT
GAAGTCGGAAATAGTTTCCGCGGATTCGAGGCAGGTCTATCGCGGAATGGATATCGGAACGGGAAAGGATCTTGATGAAT
ACCGCATCGATGGAGAGGAAATTCCCTGCCATCTGATCGACATTGTTGATCCGGATTACGATTTCAACGTTTTCGAATAT
CAGTCCCGTTTCTACCGGTGCTTCGAGGAAATTCTTTCCCGAGGGATTGTGCCCATCCTGGTGGGCGGAACCGGTTTGTA
CCTTTCCGCCGTCCTTGAAAACTACCGCATGGTGCAAGTCCCGGAAAACCTGGACTTGCGGGAATCCCTGAAGGCAGAGC
CGCTGGAGCGGTTGCAGCAGATACTTCTGGAGATAACCCCCCGCATCCACAATACAACGGATCTTCTGGATCGCGGCCGT
CTTCTCCGTGCCATTGAAATCGCACAGCATTCCGGAAGGCGGGGTTTGCGGGAAAGCCCTGAACATCCCCGCATTGAGCC
ACTGGTTTTTGGAGTGCGGTGGAATCGTGATCTTCTCCGGAAACGGATTGCCCTGCGTCTTAAGGAGCGGCTGTCCGCAG
GGCTGATCGATGAGGTAAAGGAACTGCATCAATCCGGCATTTCATGGGACAGGCTCGAATTCTTTGGTTTGGAATACCGC
TATGTCGGTCTATATCTCCAAAGCAGGATGTCCTACCGGGAAATGGCTGAAAAACTGACGATCCACATCTGCCGGTTCGC
AAAGAGGCAGGAAACATGGTTTCGACGCATGGAACGCCATGGCATCGAAATTATCTGGATCGAGGGGGATGATTACGAAG
CATTGAAAGAACAGTTAAAGGGGAATCTGCGATCATGA

Upstream 100 bases:

>100_bases
CGGCGGCCAGGCTTCAAAGGAGCTTTGCCTTCCTTCTGTTACCGTTAGAACAGTTCCTGCTAACATTGATTGCCTTGAAA
GTTCAAGAGTATTTAAAGAA

Downstream 100 bases:

>100_bases
CCCTGGCACGGAACGTTTCTGATTATTTGCGCAAATGCCGTTTGGAGGTCGCGCCTTCCCTCATTTCCGGTTCCGATTCG
TTCCGGAATCTATCACAGGT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase 1; DMAPP:tRNA dimethylallyltransferase 1; DMATase 1; Isopentenyl-diphosphate:tRNA isopentenyltransferase 1; IPP transferase 1; IPPT 1; IPTase 1

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MMKNLVVILGPTASGKTRLAVRLARDLKSEIVSADSRQVYRGMDIGTGKDLDEYRIDGEEIPCHLIDIVDPDYDFNVFEY
QSRFYRCFEEILSRGIVPILVGGTGLYLSAVLENYRMVQVPENLDLRESLKAEPLERLQQILLEITPRIHNTTDLLDRGR
LLRAIEIAQHSGRRGLRESPEHPRIEPLVFGVRWNRDLLRKRIALRLKERLSAGLIDEVKELHQSGISWDRLEFFGLEYR
YVGLYLQSRMSYREMAEKLTIHICRFAKRQETWFRRMERHGIEIIWIEGDDYEALKEQLKGNLRS

Sequences:

>Translated_305_residues
MMKNLVVILGPTASGKTRLAVRLARDLKSEIVSADSRQVYRGMDIGTGKDLDEYRIDGEEIPCHLIDIVDPDYDFNVFEY
QSRFYRCFEEILSRGIVPILVGGTGLYLSAVLENYRMVQVPENLDLRESLKAEPLERLQQILLEITPRIHNTTDLLDRGR
LLRAIEIAQHSGRRGLRESPEHPRIEPLVFGVRWNRDLLRKRIALRLKERLSAGLIDEVKELHQSGISWDRLEFFGLEYR
YVGLYLQSRMSYREMAEKLTIHICRFAKRQETWFRRMERHGIEIIWIEGDDYEALKEQLKGNLRS
>Mature_305_residues
MMKNLVVILGPTASGKTRLAVRLARDLKSEIVSADSRQVYRGMDIGTGKDLDEYRIDGEEIPCHLIDIVDPDYDFNVFEY
QSRFYRCFEEILSRGIVPILVGGTGLYLSAVLENYRMVQVPENLDLRESLKAEPLERLQQILLEITPRIHNTTDLLDRGR
LLRAIEIAQHSGRRGLRESPEHPRIEPLVFGVRWNRDLLRKRIALRLKERLSAGLIDEVKELHQSGISWDRLEFFGLEYR
YVGLYLQSRMSYREMAEKLTIHICRFAKRQETWFRRMERHGIEIIWIEGDDYEALKEQLKGNLRS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=312, Percent_Identity=26.9230769230769, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1790613, Length=313, Percent_Identity=31.9488817891374, Blast_Score=114, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI25144712, Length=257, Percent_Identity=24.5136186770428, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324848, Length=299, Percent_Identity=25.0836120401338, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24649830, Length=243, Percent_Identity=29.6296296296296, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA1_SYNAS (Q2LTI8)

Other databases:

- EMBL:   CP000252
- RefSeq:   YP_461567.1
- ProteinModelPortal:   Q2LTI8
- SMR:   Q2LTI8
- STRING:   Q2LTI8
- GeneID:   3884324
- GenomeReviews:   CP000252_GR
- KEGG:   sat:SYN_01969
- NMPDR:   fig|56780.10.peg.1511
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   IYYGLEY
- ProtClustDB:   PRK00091
- BioCyc:   SACI56780:SYN_01969-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35758; Mature: 35758

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKNLVVILGPTASGKTRLAVRLARDLKSEIVSADSRQVYRGMDIGTGKDLDEYRIDGEE
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHCCCCCC
IPCHLIDIVDPDYDFNVFEYQSRFYRCFEEILSRGIVPILVGGTGLYLSAVLENYRMVQV
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCEEEEC
PENLDLRESLKAEPLERLQQILLEITPRIHNTTDLLDRGRLLRAIEIAQHSGRRGLRESP
CCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EHPRIEPLVFGVRWNRDLLRKRIALRLKERLSAGLIDEVKELHQSGISWDRLEFFGLEYR
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHCCHHH
YVGLYLQSRMSYREMAEKLTIHICRFAKRQETWFRRMERHGIEIIWIEGDDYEALKEQLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHC
GNLRS
CCCCC
>Mature Secondary Structure
MMKNLVVILGPTASGKTRLAVRLARDLKSEIVSADSRQVYRGMDIGTGKDLDEYRIDGEE
CCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCHHHCCCCCC
IPCHLIDIVDPDYDFNVFEYQSRFYRCFEEILSRGIVPILVGGTGLYLSAVLENYRMVQV
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCEEEEC
PENLDLRESLKAEPLERLQQILLEITPRIHNTTDLLDRGRLLRAIEIAQHSGRRGLRESP
CCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EHPRIEPLVFGVRWNRDLLRKRIALRLKERLSAGLIDEVKELHQSGISWDRLEFFGLEYR
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHCCHHH
YVGLYLQSRMSYREMAEKLTIHICRFAKRQETWFRRMERHGIEIIWIEGDDYEALKEQLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHC
GNLRS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA