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The map label for this gene is flp [H]

Identifier: 85858308

GI number: 85858308

Start: 477136

End: 477825

Strand: Direct

Name: flp [H]

Synonym: SYN_00248

Alternate gene names: 85858308

Gene position: 477136-477825 (Clockwise)

Preceding gene: 85858303

Following gene: 85858313

Centisome position: 15.01

GC content: 56.23

Gene sequence:

>690_bases
GTGGACAAGGTTCTGGAAATTATCGGGTCAATTCCTCTTTTCAACGGGCTTTCCGAGCAACAACTCGGCGAGATCCGGGG
CATTGCGCGGGACCGCTTTTACGATAAGGGGAAAGAGATCTTTGCCGAGGGGGATGAGGGGGGCGGATTTTATATCGTGG
CCTCCGGGCAGGTGAAAGTCTTCAAGCTCTCCCCGGAGGGAAAGGAGCATATCCTTCATATCTACGGGCCGGGCCATTCC
TTCGGCGAGGTGCCCGTCTTTTCCGGAGAGCGATTCCCCGCCAGCGCGGAAACACTCCTGAAAAGCCACCTACTTTTCTT
CCCCCGGACGGACTTTGTGGCGCTGCTCTCCGGCAACCCCTCCCTTTGCATGAATCTGCTGGCCGATCTTTCCCTGCGCC
TGCGGCAGTTTACGATTCAGATCGAGAACCTGACCCTCAAGGAAGTTCCCGGACGACTGGCCTCCTACCTGCTCACCCTC
AGCGACGAAGAAGGACAGGGGAACTCTCTGTCCCTCAATATCTCCAAGGGGCAACTGGCCGGCCTCCTGGGGACGATCCC
GGAAACACTTTCGCGTATTTTCCTTAAAATGAACAACGCCGGTCTCATCGAGGTCAAGGGAAAGGAAATCACGATTCTGG
ACAGAAATGGCTTGCAGGCACTGGCGGAAACAGGAAAATTCGGCGGTTAG

Upstream 100 bases:

>100_bases
GTCCCCTTGATTTTCAAAGCCATTATCTCATAATGGCTTTATTGCACCTTGACCTGGATCAAGTGGAGGGCAAATTTTTT
TCCAATGAGTGGGGTGAATT

Downstream 100 bases:

>100_bases
TCCGCCATCCGGGAGAGTTGGCGGCTCCATTCTTCAAGCCGGGCTGAATCGACGGACACTTCCCGATAGATATGACGGAT
AAGATCAGGCGTGAGCCGCT

Product: cyclic nucleotide-binding domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MDKVLEIIGSIPLFNGLSEQQLGEIRGIARDRFYDKGKEIFAEGDEGGGFYIVASGQVKVFKLSPEGKEHILHIYGPGHS
FGEVPVFSGERFPASAETLLKSHLLFFPRTDFVALLSGNPSLCMNLLADLSLRLRQFTIQIENLTLKEVPGRLASYLLTL
SDEEGQGNSLSLNISKGQLAGLLGTIPETLSRIFLKMNNAGLIEVKGKEITILDRNGLQALAETGKFGG

Sequences:

>Translated_229_residues
MDKVLEIIGSIPLFNGLSEQQLGEIRGIARDRFYDKGKEIFAEGDEGGGFYIVASGQVKVFKLSPEGKEHILHIYGPGHS
FGEVPVFSGERFPASAETLLKSHLLFFPRTDFVALLSGNPSLCMNLLADLSLRLRQFTIQIENLTLKEVPGRLASYLLTL
SDEEGQGNSLSLNISKGQLAGLLGTIPETLSRIFLKMNNAGLIEVKGKEITILDRNGLQALAETGKFGG
>Mature_229_residues
MDKVLEIIGSIPLFNGLSEQQLGEIRGIARDRFYDKGKEIFAEGDEGGGFYIVASGQVKVFKLSPEGKEHILHIYGPGHS
FGEVPVFSGERFPASAETLLKSHLLFFPRTDFVALLSGNPSLCMNLLADLSLRLRQFTIQIENLTLKEVPGRLASYLLTL
SDEEGQGNSLSLNISKGQLAGLLGTIPETLSRIFLKMNNAGLIEVKGKEITILDRNGLQALAETGKFGG

Specific function: Global Transcription Factor That Controls The Expression Of Over 100 Target Genes In Response To Anoxia. It Facilitates The Adaptation To Anaerobic Growth Conditions By Regulating The Expression Of Gene Products That Are Involved In Anaerobic Energy Metab

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=211, Percent_Identity=27.9620853080569, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789756, Length=183, Percent_Identity=26.2295081967213, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24922; Mature: 24922

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKVLEIIGSIPLFNGLSEQQLGEIRGIARDRFYDKGKEIFAEGDEGGGFYIVASGQVKV
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCEEE
FKLSPEGKEHILHIYGPGHSFGEVPVFSGERFPASAETLLKSHLLFFPRTDFVALLSGNP
EEECCCCCEEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCH
SLCMNLLADLSLRLRQFTIQIENLTLKEVPGRLASYLLTLSDEEGQGNSLSLNISKGQLA
HHHHHHHHHHHHEEEEEEEEEECEEHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCHHH
GLLGTIPETLSRIFLKMNNAGLIEVKGKEITILDRNGLQALAETGKFGG
HHHHHHHHHHHHHHEEECCCCEEEECCCEEEEEECCCHHHHHHCCCCCC
>Mature Secondary Structure
MDKVLEIIGSIPLFNGLSEQQLGEIRGIARDRFYDKGKEIFAEGDEGGGFYIVASGQVKV
CCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEECCCEEE
FKLSPEGKEHILHIYGPGHSFGEVPVFSGERFPASAETLLKSHLLFFPRTDFVALLSGNP
EEECCCCCEEEEEEECCCCCCCCCEEECCCCCCCHHHHHHHHHHEEECCCCEEEEECCCH
SLCMNLLADLSLRLRQFTIQIENLTLKEVPGRLASYLLTLSDEEGQGNSLSLNISKGQLA
HHHHHHHHHHHHEEEEEEEEEECEEHHHHHHHHHHHEEEEECCCCCCCEEEEEECCCHHH
GLLGTIPETLSRIFLKMNNAGLIEVKGKEITILDRNGLQALAETGKFGG
HHHHHHHHHHHHHHEEECCCCEEEECCCEEEEEECCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113923; 8441692 [H]