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The map label for this gene is fhlA [H]

Identifier: 85858251

GI number: 85858251

Start: 410684

End: 412621

Strand: Direct

Name: fhlA [H]

Synonym: SYN_01122

Alternate gene names: 85858251

Gene position: 410684-412621 (Clockwise)

Preceding gene: 85858249

Following gene: 85858256

Centisome position: 12.92

GC content: 56.14

Gene sequence:

>1938_bases
TTGAAGGGCGTGGCGAAGTGCAGAAAACACGGCAACAGAAAAGAAGGAAAGGTGGATAGAGCTTTGGCATCTCCGGTTTC
ACACAAAAAATCCGGCATGAAGAAATGGGCGGACAATGTCGTCCCGATCCGGGATTCCAGACCGTTTTCCGCCGGTTATC
ACGATCTTGTTGACAATGCCCGAATCGGCCTTTTCAGAACCAATCTCAAAGGCGATATTCTTTATGTCAACAAATGCGGT
CTGGCGATGCTGGGTTTAGAAAATCTCGATGAGGCCAAGCCGGGAGACGCTCTCGCCTGGTATCGGAATCCGGAGGACCG
GAAAACCTTCCTGGAAAGACTGGGGAAAACCGGCCGGATCGATCAGTTTGAGGCGGAATGTGTCACGGCAAAAAGGGAAT
CCATTGTTCTGCTGGTCAATGCCGTCCTTGAAGGGGACGTTATAACGGGGGCGATGATCGATATTTCCGCTTTGAAGCAC
AAGCACGAGGCCCTCATCAGGAGGGAGAAAAGGCTGGCCGACGCCCAGCGGATCGCCCATCTGGGCTGCTGGGACTGGGA
CATCGTCACGGGGGAACTGTTCTGGTCCGATGAGGTCTATCGGATCTTCGGGCTGCGTCCCCAGGAATTCGGCGCGACCT
ATGAGGATTTTCTCGCCCGGATCCATCCCGACGACCGCCGGACCGTCGAAGAGCGTGTGAATTTGTCCCTTGCCGATCCC
GGTATCCCTTACTCGGTGAGTCACCGGATACTCCTTCCTGATGGAGCAGAATGCAAAGTATTCGAAAAGGGTGAGGTGAC
CTTTGACGGATTCGGCAAGCCGGTGCGCATGATCGGGACTGTCCACCCGCTTATTATGCGAAAACAGACCGAGGAGGAAC
TGATTCGTGCCTATGAGGAGATCCAGGCGCTGAAGGAAAAACTGGAGGCCGAAAACACCTATCTTCGTGAAGAGGTGGGT
CTGCAGCATGGCGCCGGGGAAATCATCGGTGACAGCGAGGCGATCAAAAACACTATGCACAGGGCGCTTCAGGTGGCGCG
CACTTCGGTGACGGTGCTTCTGACGGGTGAGACGGGTACGGGAAAGGGGATTTTCGCCCATTTCCTTCACCAGAACAGCG
GCCGTGCGGACAAACCCTTCGTCCACGTCAACTGCGCCGGATTGCCCCACAACCTGATCGAGAGCGAACTTTTCGGCAGG
GAAAAGGGGGCGTTCACCGGTTCGACGGCACGGCAGATCGGCCGCTTTGAACTGGCCCACGGGGGAACCATTTTTCTCGA
TGAGATTGGTGAGCTTCCCCTGGAGCTGCAGGCGAAGCTTCTCAGAGTCATCGAGGACGGGAAATTCGAGCGCCTCGGCA
GCCCGCATTCCATCAAGGTGGACGCGCGGATCATCACCTCGACCAACCGCAATCTCGAGGACGAGATCAGCGAAGGGCGG
TTTCGGGAGGATCTTTTTTTCCGGCTCAGTGTTTTTCCGATCATCATCCCGCCCCTGCGCGAGAGAAAAGCGGACATTCC
TCTTCTGGTAGAATTTTTTACTCATAAATGCAGCAAGGTTCACAGAAAAAACATTCGCCACATCCCCGCGCAGACCATGA
AGTCCCTCGAAAATTACGGATGGCCGGGAAACGTCCGGGAGCTGATGAACGTGATCGAGCGGGCGGTGATCGTCACCCAG
GGGACCGAACTCCAGCTTGCCGGACAACTCAGCGCCCACTCTATCAGATCCAGGAAAGAGAGGGGGATCGAGGGTTCGGA
AAAGCCGGCCTTCAAAGGGCTCAACGGAGTGGAACGCCGGCATATCCTCGAAACGCTTCAGGAAGCGGGATGGAAGATCG
AGGGTCCGGGAGGCGCGGCGCAGCGCCTGGAAATGAACCCGAGCACCCTGAGGACCCGGATGAAAAAACTGCAGATCAAA
CGGCCGGAAACATTATAG

Upstream 100 bases:

>100_bases
TGGCATTTTCCCCGTGATTCATGTAAAAAAAACAAGGGAATAACTGCAAGATACCATTGTCCACTATTATTCAATTCAAT
CTTTCACCTGCTCCCTGTTA

Downstream 100 bases:

>100_bases
AACCTGCCTGAAATCGCCTTCCTCTTCAATTATATTAATGCGGGGATTTCCTTCGGAGAATTCAGGGCGAGACGCTGATT
CTTCCATTCGAATCCAGCGC

Product: transcriptional regulator, sigma-54-dependent

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 645; Mature: 645

Protein sequence:

>645_residues
MKGVAKCRKHGNRKEGKVDRALASPVSHKKSGMKKWADNVVPIRDSRPFSAGYHDLVDNARIGLFRTNLKGDILYVNKCG
LAMLGLENLDEAKPGDALAWYRNPEDRKTFLERLGKTGRIDQFEAECVTAKRESIVLLVNAVLEGDVITGAMIDISALKH
KHEALIRREKRLADAQRIAHLGCWDWDIVTGELFWSDEVYRIFGLRPQEFGATYEDFLARIHPDDRRTVEERVNLSLADP
GIPYSVSHRILLPDGAECKVFEKGEVTFDGFGKPVRMIGTVHPLIMRKQTEEELIRAYEEIQALKEKLEAENTYLREEVG
LQHGAGEIIGDSEAIKNTMHRALQVARTSVTVLLTGETGTGKGIFAHFLHQNSGRADKPFVHVNCAGLPHNLIESELFGR
EKGAFTGSTARQIGRFELAHGGTIFLDEIGELPLELQAKLLRVIEDGKFERLGSPHSIKVDARIITSTNRNLEDEISEGR
FREDLFFRLSVFPIIIPPLRERKADIPLLVEFFTHKCSKVHRKNIRHIPAQTMKSLENYGWPGNVRELMNVIERAVIVTQ
GTELQLAGQLSAHSIRSRKERGIEGSEKPAFKGLNGVERRHILETLQEAGWKIEGPGGAAQRLEMNPSTLRTRMKKLQIK
RPETL

Sequences:

>Translated_645_residues
MKGVAKCRKHGNRKEGKVDRALASPVSHKKSGMKKWADNVVPIRDSRPFSAGYHDLVDNARIGLFRTNLKGDILYVNKCG
LAMLGLENLDEAKPGDALAWYRNPEDRKTFLERLGKTGRIDQFEAECVTAKRESIVLLVNAVLEGDVITGAMIDISALKH
KHEALIRREKRLADAQRIAHLGCWDWDIVTGELFWSDEVYRIFGLRPQEFGATYEDFLARIHPDDRRTVEERVNLSLADP
GIPYSVSHRILLPDGAECKVFEKGEVTFDGFGKPVRMIGTVHPLIMRKQTEEELIRAYEEIQALKEKLEAENTYLREEVG
LQHGAGEIIGDSEAIKNTMHRALQVARTSVTVLLTGETGTGKGIFAHFLHQNSGRADKPFVHVNCAGLPHNLIESELFGR
EKGAFTGSTARQIGRFELAHGGTIFLDEIGELPLELQAKLLRVIEDGKFERLGSPHSIKVDARIITSTNRNLEDEISEGR
FREDLFFRLSVFPIIIPPLRERKADIPLLVEFFTHKCSKVHRKNIRHIPAQTMKSLENYGWPGNVRELMNVIERAVIVTQ
GTELQLAGQLSAHSIRSRKERGIEGSEKPAFKGLNGVERRHILETLQEAGWKIEGPGGAAQRLEMNPSTLRTRMKKLQIK
RPETL
>Mature_645_residues
MKGVAKCRKHGNRKEGKVDRALASPVSHKKSGMKKWADNVVPIRDSRPFSAGYHDLVDNARIGLFRTNLKGDILYVNKCG
LAMLGLENLDEAKPGDALAWYRNPEDRKTFLERLGKTGRIDQFEAECVTAKRESIVLLVNAVLEGDVITGAMIDISALKH
KHEALIRREKRLADAQRIAHLGCWDWDIVTGELFWSDEVYRIFGLRPQEFGATYEDFLARIHPDDRRTVEERVNLSLADP
GIPYSVSHRILLPDGAECKVFEKGEVTFDGFGKPVRMIGTVHPLIMRKQTEEELIRAYEEIQALKEKLEAENTYLREEVG
LQHGAGEIIGDSEAIKNTMHRALQVARTSVTVLLTGETGTGKGIFAHFLHQNSGRADKPFVHVNCAGLPHNLIESELFGR
EKGAFTGSTARQIGRFELAHGGTIFLDEIGELPLELQAKLLRVIEDGKFERLGSPHSIKVDARIITSTNRNLEDEISEGR
FREDLFFRLSVFPIIIPPLRERKADIPLLVEFFTHKCSKVHRKNIRHIPAQTMKSLENYGWPGNVRELMNVIERAVIVTQ
GTELQLAGQLSAHSIRSRKERGIEGSEKPAFKGLNGVERRHILETLQEAGWKIEGPGGAAQRLEMNPSTLRTRMKKLQIK
RPETL

Specific function: Required for induction of expression of the formate dehydrogenase H and hydrogenase-3 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789087, Length=346, Percent_Identity=47.9768786127168, Blast_Score=310, Evalue=2e-85,
Organism=Escherichia coli, GI87082117, Length=357, Percent_Identity=44.5378151260504, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI1788550, Length=312, Percent_Identity=42.3076923076923, Blast_Score=251, Evalue=1e-67,
Organism=Escherichia coli, GI1790299, Length=246, Percent_Identity=45.9349593495935, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1790437, Length=265, Percent_Identity=43.0188679245283, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87082152, Length=236, Percent_Identity=47.0338983050847, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788905, Length=234, Percent_Identity=45.2991452991453, Blast_Score=221, Evalue=2e-58,
Organism=Escherichia coli, GI1789233, Length=239, Percent_Identity=45.1882845188285, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI1786524, Length=247, Percent_Identity=44.5344129554656, Blast_Score=204, Evalue=2e-53,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=43.4782608695652, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1787583, Length=240, Percent_Identity=41.25, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI87081858, Length=351, Percent_Identity=28.4900284900285, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI1789828, Length=228, Percent_Identity=35.0877192982456, Blast_Score=126, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 72667; Mature: 72667

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGVAKCRKHGNRKEGKVDRALASPVSHKKSGMKKWADNVVPIRDSRPFSAGYHDLVDNA
CCCHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHCCC
RIGLFRTNLKGDILYVNKCGLAMLGLENLDEAKPGDALAWYRNPEDRKTFLERLGKTGRI
CEEEEEECCCCCEEEEECCCEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCC
DQFEAECVTAKRESIVLLVNAVLEGDVITGAMIDISALKHKHEALIRREKRLADAQRIAH
CCHHHHHHHHCCCCEEEEEEHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGCWDWDIVTGELFWSDEVYRIFGLRPQEFGATYEDFLARIHPDDRRTVEERVNLSLADP
CCCCCCEEEEEEEEECCCEEHEECCCHHHHCCCHHHHHHHCCCCHHHHHHHHCCEEECCC
GIPYSVSHRILLPDGAECKVFEKGEVTFDGFGKPVRMIGTVHPLIMRKQTEEELIRAYEE
CCCCCCCCEEECCCCCCEEEEECCCEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
IQALKEKLEAENTYLREEVGLQHGAGEIIGDSEAIKNTMHRALQVARTSVTVLLTGETGT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCC
GKGIFAHFLHQNSGRADKPFVHVNCAGLPHNLIESELFGREKGAFTGSTARQIGRFELAH
CCHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHCCEEECC
GGTIFLDEIGELPLELQAKLLRVIEDGKFERLGSPHSIKVDARIITSTNRNLEDEISEGR
CCEEEEECCCCCCHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEEEECCCCCCHHHHHCCC
FREDLFFRLSVFPIIIPPLRERKADIPLLVEFFTHKCSKVHRKNIRHIPAQTMKSLENYG
HHHHHHHHEEHHEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
WPGNVRELMNVIERAVIVTQGTELQLAGQLSAHSIRSRKERGIEGSEKPAFKGLNGVERR
CCCCHHHHHHHHHHHEEEECCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
HILETLQEAGWKIEGPGGAAQRLEMNPSTLRTRMKKLQIKRPETL
HHHHHHHHCCCEEECCCCCCCEEECCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKGVAKCRKHGNRKEGKVDRALASPVSHKKSGMKKWADNVVPIRDSRPFSAGYHDLVDNA
CCCHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHCCC
RIGLFRTNLKGDILYVNKCGLAMLGLENLDEAKPGDALAWYRNPEDRKTFLERLGKTGRI
CEEEEEECCCCCEEEEECCCEEECCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCC
DQFEAECVTAKRESIVLLVNAVLEGDVITGAMIDISALKHKHEALIRREKRLADAQRIAH
CCHHHHHHHHCCCCEEEEEEHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGCWDWDIVTGELFWSDEVYRIFGLRPQEFGATYEDFLARIHPDDRRTVEERVNLSLADP
CCCCCCEEEEEEEEECCCEEHEECCCHHHHCCCHHHHHHHCCCCHHHHHHHHCCEEECCC
GIPYSVSHRILLPDGAECKVFEKGEVTFDGFGKPVRMIGTVHPLIMRKQTEEELIRAYEE
CCCCCCCCEEECCCCCCEEEEECCCEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
IQALKEKLEAENTYLREEVGLQHGAGEIIGDSEAIKNTMHRALQVARTSVTVLLTGETGT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEECCCCC
GKGIFAHFLHQNSGRADKPFVHVNCAGLPHNLIESELFGREKGAFTGSTARQIGRFELAH
CCHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCHHHHHHCCEEECC
GGTIFLDEIGELPLELQAKLLRVIEDGKFERLGSPHSIKVDARIITSTNRNLEDEISEGR
CCEEEEECCCCCCHHHHHHHHHHHHCCCHHHCCCCCEEEEEEEEEECCCCCCHHHHHCCC
FREDLFFRLSVFPIIIPPLRERKADIPLLVEFFTHKCSKVHRKNIRHIPAQTMKSLENYG
HHHHHHHHEEHHEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
WPGNVRELMNVIERAVIVTQGTELQLAGQLSAHSIRSRKERGIEGSEKPAFKGLNGVERR
CCCCHHHHHHHHHHHEEEECCCCEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
HILETLQEAGWKIEGPGGAAQRLEMNPSTLRTRMKKLQIKRPETL
HHHHHHHHCCCEEECCCCCCCEEECCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2280686; 2118503; 9278503 [H]