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The map label for this gene is purU

Identifier: 85375737

GI number: 85375737

Start: 2929098

End: 2929952

Strand: Direct

Name: purU

Synonym: ELI_14550

Alternate gene names: 85375737

Gene position: 2929098-2929952 (Clockwise)

Preceding gene: 85375736

Following gene: 85375738

Centisome position: 95.96

GC content: 62.57

Gene sequence:

>855_bases
GTGAGCGATCCGCTCGTCCTGACGCTGTCCTGCGCCGACCGGCCCGGCATCACCGCCAATGTCACCGGGTTCCTGTTCGA
GCATGGCTGCAACATTCTCGAAGCGCGCCAGTTCAACGATCGCGAAAGCGACCGCTTTTTCATGCGGATCGCCTTCGATC
CGCTGGAGGCCGACCCCGATGCCCTCAAGCGCGACTTTGCCGCCTATGCCGACCGCCTCGGCATGGACTGGAAGCTGGCG
AAACGCGATCGCAGACGGCGGGTGCTGATCATGGTCAGCAGGTTCGACCATTGCCTCGCCGACCTGCTCTACCGCTGGCG
TATCGGCGAATTACCGATCGAGCCCGTCGCGATAGTCTCCAACCATCCGCGCGAGGCGATCAGCCACACGCATATCGGCG
AAGTGCCGTTCCATCACCTGCCGGTCACGCACGAGACCAAGCTCGATCAGGAGGCGCAGGTCCGCGCCATCGCCGAAGAG
ACCGACACAGAACTCGTCGTGCTTGCCCGCTACATGCAGATTCTCTCGGACGAGCAGGCAGCGCATTTTGCCGCGCGTTG
CATCAACATCCACCACTCCTTCCTGCCGGGCTTCAAGGGGGCAAAACCCTATCACCAGGCGCATGCGCGGGGAGTGAAGA
TGATCGGTGCTACGGCACACTACGTGACCACCGATCTCGACGAAGGCCCCATCATCCACCAGGATGTCGAGCCGATCAGC
CATGCCGACAGTCCCGAAGACCTGGTCCGCAAGGGCCGCGATATCGAAAGCCGGGTGCTGGCCGAAGCCGTGCGGATGCA
TGTCGAAGAGCGGGTGTTGATCAACGGTCAGCGCACGATCGTCTTTCGCAGCTAG

Upstream 100 bases:

>100_bases
CTCGCGGTTCGCGTACTTCACACGGCCTATGGGCTGGATGCGGAGGACGCGGCTGCGTGAGCGGCTCTTGCGGAGATACA
ATCGCCGGAGTGGCCCTCGT

Downstream 100 bases:

>100_bases
TGCTCCCCAACGAGAGGCAGATCATGTGCAACGATCGGCTTCCCCGCATCTGTAACAAGCGCTGGTCGCGAGCGATTTCG
CTGATATGGTAGCGAAATGA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSDPLVLTLSCADRPGITANVTGFLFEHGCNILEARQFNDRESDRFFMRIAFDPLEADPDALKRDFAAYADRLGMDWKLA
KRDRRRRVLIMVSRFDHCLADLLYRWRIGELPIEPVAIVSNHPREAISHTHIGEVPFHHLPVTHETKLDQEAQVRAIAEE
TDTELVVLARYMQILSDEQAAHFAARCINIHHSFLPGFKGAKPYHQAHARGVKMIGATAHYVTTDLDEGPIIHQDVEPIS
HADSPEDLVRKGRDIESRVLAEAVRMHVEERVLINGQRTIVFRS

Sequences:

>Translated_284_residues
MSDPLVLTLSCADRPGITANVTGFLFEHGCNILEARQFNDRESDRFFMRIAFDPLEADPDALKRDFAAYADRLGMDWKLA
KRDRRRRVLIMVSRFDHCLADLLYRWRIGELPIEPVAIVSNHPREAISHTHIGEVPFHHLPVTHETKLDQEAQVRAIAEE
TDTELVVLARYMQILSDEQAAHFAARCINIHHSFLPGFKGAKPYHQAHARGVKMIGATAHYVTTDLDEGPIIHQDVEPIS
HADSPEDLVRKGRDIESRVLAEAVRMHVEERVLINGQRTIVFRS
>Mature_283_residues
SDPLVLTLSCADRPGITANVTGFLFEHGCNILEARQFNDRESDRFFMRIAFDPLEADPDALKRDFAAYADRLGMDWKLAK
RDRRRRVLIMVSRFDHCLADLLYRWRIGELPIEPVAIVSNHPREAISHTHIGEVPFHHLPVTHETKLDQEAQVRAIAEET
DTELVVLARYMQILSDEQAAHFAARCINIHHSFLPGFKGAKPYHQAHARGVKMIGATAHYVTTDLDEGPIIHQDVEPISH
ADSPEDLVRKGRDIESRVLAEAVRMHVEERVLINGQRTIVFRS

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=123, Percent_Identity=30.0813008130081, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI209869995, Length=123, Percent_Identity=30.0813008130081, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI209869993, Length=123, Percent_Identity=30.0813008130081, Blast_Score=78, Evalue=8e-15,
Organism=Escherichia coli, GI1787483, Length=281, Percent_Identity=39.5017793594306, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1788846, Length=140, Percent_Identity=33.5714285714286, Blast_Score=72, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17567511, Length=177, Percent_Identity=31.638418079096, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32360; Mature: 32229

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPLVLTLSCADRPGITANVTGFLFEHGCNILEARQFNDRESDRFFMRIAFDPLEADPD
CCCCEEEEEECCCCCCCEEHHHHHHHHHCCCEEEHHCCCCCCCCEEEEEEEECCCCCCHH
ALKRDFAAYADRLGMDWKLAKRDRRRRVLIMVSRFDHCLADLLYRWRIGELPIEPVAIVS
HHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEC
NHPREAISHTHIGEVPFHHLPVTHETKLDQEAQVRAIAEETDTELVVLARYMQILSDEQA
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHH
AHFAARCINIHHSFLPGFKGAKPYHQAHARGVKMIGATAHYVTTDLDEGPIIHQDVEPIS
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECEEHEEEECCCCCCEECCCCCHHC
HADSPEDLVRKGRDIESRVLAEAVRMHVEERVLINGQRTIVFRS
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEEC
>Mature Secondary Structure 
SDPLVLTLSCADRPGITANVTGFLFEHGCNILEARQFNDRESDRFFMRIAFDPLEADPD
CCCEEEEEECCCCCCCEEHHHHHHHHHCCCEEEHHCCCCCCCCEEEEEEEECCCCCCHH
ALKRDFAAYADRLGMDWKLAKRDRRRRVLIMVSRFDHCLADLLYRWRIGELPIEPVAIVS
HHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCEEEEC
NHPREAISHTHIGEVPFHHLPVTHETKLDQEAQVRAIAEETDTELVVLARYMQILSDEQA
CCCHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHH
AHFAARCINIHHSFLPGFKGAKPYHQAHARGVKMIGATAHYVTTDLDEGPIIHQDVEPIS
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCEEEEECEEHEEEECCCCCCEECCCCCHHC
HADSPEDLVRKGRDIESRVLAEAVRMHVEERVLINGQRTIVFRS
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]