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The map label for this gene is lysC [H]

Identifier: 85375736

GI number: 85375736

Start: 2927792

End: 2929057

Strand: Direct

Name: lysC [H]

Synonym: ELI_14545

Alternate gene names: 85375736

Gene position: 2927792-2929057 (Clockwise)

Preceding gene: 85375733

Following gene: 85375737

Centisome position: 95.92

GC content: 65.01

Gene sequence:

>1266_bases
ATGAAATTCGGCGGCACCTCGATGGCGGGGACCGAGCGGATTCGCCGCGTGGCCAATATCGTGCGGAAGCAGGCGGCAGG
CGGCGACGAGGTCGCGGTGGTGGTCTCCGCCATGGCGGGCGAGACCGACCGTCTCGTCAATTTCTGCCGCGAAGCCAACG
CGCTGTACGATCCGGCGGAATACGATGTCGTGGTGGCCAGCGGGGAGCAGGTCACGTCCGGCCTGCTTGCGCTCACGCTG
CAATCACTCGGCTGCAAGGCGCGCAGCTGGCTCGGCTGGCAATTGCCGGTCCACACCGTCGAAGCGCATGCCAAGGCGCG
GATCGAGGATATCGAGAACGACGCGCTGCTGGAGAGCATGAGGGGCGGGACCATCGCCGTCATACCCGGCTTCCAGGGCC
TTTCGGACGATGGCCGGATCACCACGCTCGGCCGGGGCGGATCGGATACCTCGGCGGTAGCCGTAGCGGCCGCTATAGAT
GCGGATCGCTGCGACATCTACACCGATGTCGACGGGGTCTACACCACCGACCCGCGCATCGTCGCCCGCGCGCGCAAGCA
GAAAGCGGTGACCTACGAGGAAATGCTCGAACTGGCCTCGGTCGGGGCCAAGGTGCTGCAGACCCGCTCGGTCGGTCTGG
CGATGAAGGAGGGCGTGCGCGTGCAGGTGCTGTCCAGCTTCGTCGACGACGATGCGACACCCGCCGACGACCTGCCCGGC
ACGATGATCGTGTCGGAAGAGGAAATGGATAGGATTCTGGAGGAGGGCGATATGGAACGCCAGCTCGTGACCGGCATTGC
GCACGACAAGAACGAAGCGAAGATCATCCTGACCCGCGTGCCGGACAAGCCGGGGGCAGTCGCACACATCTTCGAGCCGC
TCGCCGCCGCCAGCATCAATGTCGACATGATCATCCAGAATGTCGGCCGCGACAAGGGCGAGACCGATGTGACCTTCACG
GTTCCGCAGGCGGACCTGGCGCGCGCGCAGGCGCTGCTGGAAGACAGGCGCGAGGAAATCGGCTTCAACCGCATCATCAC
CGACAGCCAGATCGCCAAGATCAGCGTCGTCGGCGTCGGCATGAAAAGCCACGCGGGCGTCGCCAGCACCATGTTCCGCG
CGCTATCCGACCGCGGCATCAATATCCAGGCAATCTCGACTTCCGAGATCAAGGTCAGCGTCATGATCGACGAGGACGAG
ACCGAGCTCGCGGTTCGCGTACTTCACACGGCCTATGGGCTGGATGCGGAGGACGCGGCTGCGTGA

Upstream 100 bases:

>100_bases
GTAAACCGCAAGCTTGCTTGTGTTTGGCGCGCACGACCCTTAGTCCCCTGCGCTCTCGCTTGTTCAGCATCTGGGGGCCT
GCACCTTGGCACGCATCGTG

Downstream 100 bases:

>100_bases
GCGGCTCTTGCGGAGATACAATCGCCGGAGTGGCCCTCGTGTGAGCGATCCGCTCGTCCTGACGCTGTCCTGCGCCGACC
GGCCCGGCATCACCGCCAAT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MKFGGTSMAGTERIRRVANIVRKQAAGGDEVAVVVSAMAGETDRLVNFCREANALYDPAEYDVVVASGEQVTSGLLALTL
QSLGCKARSWLGWQLPVHTVEAHAKARIEDIENDALLESMRGGTIAVIPGFQGLSDDGRITTLGRGGSDTSAVAVAAAID
ADRCDIYTDVDGVYTTDPRIVARARKQKAVTYEEMLELASVGAKVLQTRSVGLAMKEGVRVQVLSSFVDDDATPADDLPG
TMIVSEEEMDRILEEGDMERQLVTGIAHDKNEAKIILTRVPDKPGAVAHIFEPLAAASINVDMIIQNVGRDKGETDVTFT
VPQADLARAQALLEDRREEIGFNRIITDSQIAKISVVGVGMKSHAGVASTMFRALSDRGINIQAISTSEIKVSVMIDEDE
TELAVRVLHTAYGLDAEDAAA

Sequences:

>Translated_421_residues
MKFGGTSMAGTERIRRVANIVRKQAAGGDEVAVVVSAMAGETDRLVNFCREANALYDPAEYDVVVASGEQVTSGLLALTL
QSLGCKARSWLGWQLPVHTVEAHAKARIEDIENDALLESMRGGTIAVIPGFQGLSDDGRITTLGRGGSDTSAVAVAAAID
ADRCDIYTDVDGVYTTDPRIVARARKQKAVTYEEMLELASVGAKVLQTRSVGLAMKEGVRVQVLSSFVDDDATPADDLPG
TMIVSEEEMDRILEEGDMERQLVTGIAHDKNEAKIILTRVPDKPGAVAHIFEPLAAASINVDMIIQNVGRDKGETDVTFT
VPQADLARAQALLEDRREEIGFNRIITDSQIAKISVVGVGMKSHAGVASTMFRALSDRGINIQAISTSEIKVSVMIDEDE
TELAVRVLHTAYGLDAEDAAA
>Mature_421_residues
MKFGGTSMAGTERIRRVANIVRKQAAGGDEVAVVVSAMAGETDRLVNFCREANALYDPAEYDVVVASGEQVTSGLLALTL
QSLGCKARSWLGWQLPVHTVEAHAKARIEDIENDALLESMRGGTIAVIPGFQGLSDDGRITTLGRGGSDTSAVAVAAAID
ADRCDIYTDVDGVYTTDPRIVARARKQKAVTYEEMLELASVGAKVLQTRSVGLAMKEGVRVQVLSSFVDDDATPADDLPG
TMIVSEEEMDRILEEGDMERQLVTGIAHDKNEAKIILTRVPDKPGAVAHIFEPLAAASINVDMIIQNVGRDKGETDVTFT
VPQADLARAQALLEDRREEIGFNRIITDSQIAKISVVGVGMKSHAGVASTMFRALSDRGINIQAISTSEIKVSVMIDEDE
TELAVRVLHTAYGLDAEDAAA

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=294, Percent_Identity=34.3537414965986, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1790455, Length=464, Percent_Identity=26.5086206896552, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790376, Length=355, Percent_Identity=24.5070422535211, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320893, Length=374, Percent_Identity=25.668449197861, Blast_Score=106, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 45041; Mature: 45041

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFGGTSMAGTERIRRVANIVRKQAAGGDEVAVVVSAMAGETDRLVNFCREANALYDPAE
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCHHHHHHHHHHCCCCCCCCC
YDVVVASGEQVTSGLLALTLQSLGCKARSWLGWQLPVHTVEAHAKARIEDIENDALLESM
CEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
RGGTIAVIPGFQGLSDDGRITTLGRGGSDTSAVAVAAAIDADRCDIYTDVDGVYTTDPRI
CCCEEEEECCCCCCCCCCCEEEECCCCCCCHHEEEEEEECCCCCEEEECCCCEEECCHHH
VARARKQKAVTYEEMLELASVGAKVLQTRSVGLAMKEGVRVQVLSSFVDDDATPADDLPG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCCCCCCCC
TMIVSEEEMDRILEEGDMERQLVTGIAHDKNEAKIILTRVPDKPGAVAHIFEPLAAASIN
EEEECHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCC
VDMIIQNVGRDKGETDVTFTVPQADLARAQALLEDRREEIGFNRIITDSQIAKISVVGVG
HHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEEEEC
MKSHAGVASTMFRALSDRGINIQAISTSEIKVSVMIDEDETELAVRVLHTAYGLDAEDAA
CCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHCC
A
C
>Mature Secondary Structure
MKFGGTSMAGTERIRRVANIVRKQAAGGDEVAVVVSAMAGETDRLVNFCREANALYDPAE
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHCCCHHHHHHHHHHCCCCCCCCC
YDVVVASGEQVTSGLLALTLQSLGCKARSWLGWQLPVHTVEAHAKARIEDIENDALLESM
CEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHH
RGGTIAVIPGFQGLSDDGRITTLGRGGSDTSAVAVAAAIDADRCDIYTDVDGVYTTDPRI
CCCEEEEECCCCCCCCCCCEEEECCCCCCCHHEEEEEEECCCCCEEEECCCCEEECCHHH
VARARKQKAVTYEEMLELASVGAKVLQTRSVGLAMKEGVRVQVLSSFVDDDATPADDLPG
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCCCCCCCCC
TMIVSEEEMDRILEEGDMERQLVTGIAHDKNEAKIILTRVPDKPGAVAHIFEPLAAASIN
EEEECHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCC
VDMIIQNVGRDKGETDVTFTVPQADLARAQALLEDRREEIGFNRIITDSQIAKISVVGVG
HHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEEEEEC
MKSHAGVASTMFRALSDRGINIQAISTSEIKVSVMIDEDETELAVRVLHTAYGLDAEDAA
CCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHCCCCHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]