Click here to switch to the map view.

The map label for this gene is ruvC

Identifier: 85375731

GI number: 85375731

Start: 2923893

End: 2924384

Strand: Direct

Name: ruvC

Synonym: ELI_14520

Alternate gene names: 85375731

Gene position: 2923893-2924384 (Clockwise)

Preceding gene: 85375730

Following gene: 85375732

Centisome position: 95.79

GC content: 68.29

Gene sequence:

>492_bases
GTGACCATAATCCTCGGCCTCGATCCCTCGCTCAGCTGTACCGGCTGGGGCGTCATCCGGGCCGAGGGCTCGCGTATCGC
GCATGTCGCCAACGGGCAAGTCAAGACGGATGCAAAGGCCCCGATCGCGGATCGCCTGCATCACCTGCACGCTGCCATCG
CAGCGGTGATTGCCGCACACGACCCGGACCGGGCGGCGGCGGAGGAGATCTTCATCAACAAGAACCCGCAATCGACTCTC
AAGCTGGCGCAGGCGCGGGGCTGCGTGCTGGCGGCCTGCGCGGCGGGCGGCCTGGCCGTCAACGAACACGCCGCGCGGCT
GGTCAAGAAATCCGTCGTCGGTACGGGCGGGGCGGACAAGGCCCAAGTGCAGGCCATGCTCAAGGTGCTAATGCCGGGGG
CGCAAGTCGCCGGAGCCGATGCCGCTGACGCGCTGGCCGTCGCGATCGCCGATGCGAACTTGCGGAGCATCGCGAACCTC
CGTTCGCCCTGA

Upstream 100 bases:

>100_bases
AGCACCCTCTCCCCTTCAGGGGAGAGGGCCGGGGAGTGGGGGCCATCGTCCGCATCCCCGATCCCCTCTCCCAACCCTCT
CCCCTGAAGGGAAGAGGGCA

Downstream 100 bases:

>100_bases
GCCTGTCGAAGGGCAGGCGAAACACGGCGGCTGGGCTTCGACAGGCTCAGCCCGAACGGGTGTGGGGTCTATCCCCCGGC
CCAAGCACTCGCTAGATCGC

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MTIILGLDPSLSCTGWGVIRAEGSRIAHVANGQVKTDAKAPIADRLHHLHAAIAAVIAAHDPDRAAAEEIFINKNPQSTL
KLAQARGCVLAACAAGGLAVNEHAARLVKKSVVGTGGADKAQVQAMLKVLMPGAQVAGADAADALAVAIADANLRSIANL
RSP

Sequences:

>Translated_163_residues
MTIILGLDPSLSCTGWGVIRAEGSRIAHVANGQVKTDAKAPIADRLHHLHAAIAAVIAAHDPDRAAAEEIFINKNPQSTL
KLAQARGCVLAACAAGGLAVNEHAARLVKKSVVGTGGADKAQVQAMLKVLMPGAQVAGADAADALAVAIADANLRSIANL
RSP
>Mature_162_residues
TIILGLDPSLSCTGWGVIRAEGSRIAHVANGQVKTDAKAPIADRLHHLHAAIAAVIAAHDPDRAAAEEIFINKNPQSTLK
LAQARGCVLAACAAGGLAVNEHAARLVKKSVVGTGGADKAQVQAMLKVLMPGAQVAGADAADALAVAIADANLRSIANLR
SP

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=131, Percent_Identity=41.9847328244275, Blast_Score=109, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_ERYLH (Q2N5P5)

Other databases:

- EMBL:   CP000157
- RefSeq:   YP_459793.1
- ProteinModelPortal:   Q2N5P5
- SMR:   Q2N5P5
- STRING:   Q2N5P5
- GeneID:   3871204
- GenomeReviews:   CP000157_GR
- KEGG:   eli:ELI_14520
- NMPDR:   fig|314225.3.peg.114
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   PMQIKLA
- PhylomeDB:   Q2N5P5
- ProtClustDB:   PRK00039
- BioCyc:   ELIT314225:ELI_14520-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 16529; Mature: 16398

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIILGLDPSLSCTGWGVIRAEGSRIAHVANGQVKTDAKAPIADRLHHLHAAIAAVIAAH
CEEEEECCCCCCCCCCEEEEECCCEEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHCC
DPDRAAAEEIFINKNPQSTLKLAQARGCVLAACAAGGLAVNEHAARLVKKSVVGTGGADK
CCCHHHHHHEEECCCCHHHHHHHHHCCCEEEEHHCCCEEHHHHHHHHHHHHHCCCCCCCH
AQVQAMLKVLMPGAQVAGADAADALAVAIADANLRSIANLRSP
HHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCHHHHHHHCCCC
>Mature Secondary Structure 
TIILGLDPSLSCTGWGVIRAEGSRIAHVANGQVKTDAKAPIADRLHHLHAAIAAVIAAH
EEEEECCCCCCCCCCEEEEECCCEEEEECCCCEECCCCCCHHHHHHHHHHHHHHHHHCC
DPDRAAAEEIFINKNPQSTLKLAQARGCVLAACAAGGLAVNEHAARLVKKSVVGTGGADK
CCCHHHHHHEEECCCCHHHHHHHHHCCCEEEEHHCCCEEHHHHHHHHHHHHHCCCCCCCH
AQVQAMLKVLMPGAQVAGADAADALAVAIADANLRSIANLRSP
HHHHHHHHHHCCCCCCCCCCCHHHEEEEEECCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA