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The map label for this gene is hemK [H]

Identifier: 85375681

GI number: 85375681

Start: 2865912

End: 2866763

Strand: Direct

Name: hemK [H]

Synonym: ELI_14270

Alternate gene names: 85375681

Gene position: 2865912-2866763 (Clockwise)

Preceding gene: 85375680

Following gene: 85375682

Centisome position: 93.89

GC content: 64.79

Gene sequence:

>852_bases
ATGAAAATCGCCTCGGCCCTGCGCGGGGCGACGCAGCGGTTGGGCGAAGTCTCCGACACCGCGCGGCTCGATGCCGAATA
TCTCATGGCGCATGCGGTGGGAGTAACGCGCTCCGACCTCCTGCTGAACCACCAGGGCGACGCAGTGCCCGGTGAGTTCG
CGGACTTGGTCGCGCGCCGCCTGCACCACGAACCGCTGGCCTACATCACGGGCGGGCAGGAGTTCTTCGGGCGCGGTTTC
ATCGTCACACCCGATGTCCTGATCCCGCGCAGCGACAGCGAACAGGTGGTCGAAGCCGCTTTGGCCGAAATGGGCGAGGG
TGCCCGGGTGCTGGACCTCGGCACCGGCTCGGGCGCGCTTTTGGTGACGTTGCTGGCGGAACACCCGGATGCAACCGGGA
CCGGCATCGATGCGTCGCTTGCTGCGCTGCCGGTCGCAGCGGCAAATGCTGCCCGGAACGGCGTTGCCGACCGCGCGTGC
CTGCTGAAAGCCGATTGGCGCGAGCCGGGTTGGACCGACGATCTCGGCCAGTTCGACCTCGTGATCGCCAATCCGCCCTA
TGTCGAGGACAATGCGGCGCTCGATGCTTCGGTGCGCAAGTTTGAACCTCGGGCGGCTCTGTATTCCGGCCCGGAAGGGC
TTGACGACTACCGCGCCATCATCCCGCAGCTGTCCAGTCTTTTGTCGTCCGATGGCGTTGCGGTGCTGGAAATCGGTTAT
CGACAGGCGGACGCAGTGGGTACGATAGCCGAGGAAAACGGTTTCGAATCAAGGCTCAGACATGACCTTGCAGACCGCCC
CCGGGCACTGATTCTGCGCAAAGGGGTTGGCAAAGGGAGAATCAGGGGCTAA

Upstream 100 bases:

>100_bases
CGCTTCGACGGGCTCGGGGCGAACGCAGGGAGCGCCGGCGGCTCCTCTCTACGTCCGTTCGTGCTGAGCCTGTCGAAGCA
CAGCCACAGCGTTCGAGCCC

Downstream 100 bases:

>100_bases
CTCTACTCACAAGACCTGCGGATTGCGACTTTGCTCTCCCCGGTCTTGATCCAACATTTGCGAAGATGCCGGGCGCCAGC
GCGCTTGCCAGCAATGCAGA

Product: protein chain release factor methylase subunit

Products: NA

Alternate protein names: HemK protein homolog; M.RcoHemKP; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKIASALRGATQRLGEVSDTARLDAEYLMAHAVGVTRSDLLLNHQGDAVPGEFADLVARRLHHEPLAYITGGQEFFGRGF
IVTPDVLIPRSDSEQVVEAALAEMGEGARVLDLGTGSGALLVTLLAEHPDATGTGIDASLAALPVAAANAARNGVADRAC
LLKADWREPGWTDDLGQFDLVIANPPYVEDNAALDASVRKFEPRAALYSGPEGLDDYRAIIPQLSSLLSSDGVAVLEIGY
RQADAVGTIAEENGFESRLRHDLADRPRALILRKGVGKGRIRG

Sequences:

>Translated_283_residues
MKIASALRGATQRLGEVSDTARLDAEYLMAHAVGVTRSDLLLNHQGDAVPGEFADLVARRLHHEPLAYITGGQEFFGRGF
IVTPDVLIPRSDSEQVVEAALAEMGEGARVLDLGTGSGALLVTLLAEHPDATGTGIDASLAALPVAAANAARNGVADRAC
LLKADWREPGWTDDLGQFDLVIANPPYVEDNAALDASVRKFEPRAALYSGPEGLDDYRAIIPQLSSLLSSDGVAVLEIGY
RQADAVGTIAEENGFESRLRHDLADRPRALILRKGVGKGRIRG
>Mature_283_residues
MKIASALRGATQRLGEVSDTARLDAEYLMAHAVGVTRSDLLLNHQGDAVPGEFADLVARRLHHEPLAYITGGQEFFGRGF
IVTPDVLIPRSDSEQVVEAALAEMGEGARVLDLGTGSGALLVTLLAEHPDATGTGIDASLAALPVAAANAARNGVADRAC
LLKADWREPGWTDDLGQFDLVIANPPYVEDNAALDASVRKFEPRAALYSGPEGLDDYRAIIPQLSSLLSSDGVAVLEIGY
RQADAVGTIAEENGFESRLRHDLADRPRALILRKGVGKGRIRG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=282, Percent_Identity=30.4964539007092, Blast_Score=102, Evalue=3e-22,
Organism=Escherichia coli, GI1787463, Length=252, Percent_Identity=35.3174603174603, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI87082085, Length=190, Percent_Identity=33.6842105263158, Blast_Score=88, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324266, Length=241, Percent_Identity=26.9709543568465, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24582226, Length=221, Percent_Identity=29.8642533936652, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 30040; Mature: 30040

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIASALRGATQRLGEVSDTARLDAEYLMAHAVGVTRSDLLLNHQGDAVPGEFADLVARR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCHHHHHHHHHH
LHHEPLAYITGGQEFFGRGFIVTPDVLIPRSDSEQVVEAALAEMGEGARVLDLGTGSGAL
HCCCCCEEEECCHHHHCCCEEECCCEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCE
LVTLLAEHPDATGTGIDASLAALPVAAANAARNGVADRACLLKADWREPGWTDDLGQFDL
EEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCCEEE
VIANPPYVEDNAALDASVRKFEPRAALYSGPEGLDDYRAIIPQLSSLLSSDGVAVLEIGY
EECCCCCCCCCCCHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
RQADAVGTIAEENGFESRLRHDLADRPRALILRKGVGKGRIRG
HHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCC
>Mature Secondary Structure
MKIASALRGATQRLGEVSDTARLDAEYLMAHAVGVTRSDLLLNHQGDAVPGEFADLVARR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCHHHHHHHHHH
LHHEPLAYITGGQEFFGRGFIVTPDVLIPRSDSEQVVEAALAEMGEGARVLDLGTGSGAL
HCCCCCEEEECCHHHHCCCEEECCCEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCE
LVTLLAEHPDATGTGIDASLAALPVAAANAARNGVADRACLLKADWREPGWTDDLGQFDL
EEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCCCCCCCCEEE
VIANPPYVEDNAALDASVRKFEPRAALYSGPEGLDDYRAIIPQLSSLLSSDGVAVLEIGY
EECCCCCCCCCCCHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECC
RQADAVGTIAEENGFESRLRHDLADRPRALILRKGVGKGRIRG
HHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]