Click here to switch to the map view.

The map label for this gene is tme [H]

Identifier: 85375668

GI number: 85375668

Start: 2851190

End: 2853451

Strand: Direct

Name: tme [H]

Synonym: ELI_14205

Alternate gene names: 85375668

Gene position: 2851190-2853451 (Clockwise)

Preceding gene: 85375664

Following gene: 85375669

Centisome position: 93.41

GC content: 63.31

Gene sequence:

>2262_bases
TTGGCCGAAGAAAAACCGACCGGGTTCACCGCGCGCGAAGCGCTGTTCTACCACGAGACGATCCGTCCCGGTAAAATCGA
GATCATCGCCAGTAAACCGATGGCGACCCAGCGCGACCTGAGCCTGGCCTACTCGCCGGGTGTGGCGGTACCGGTCGAAG
CGATCGCGGAAGATCCGGCCAACGCGGCCAAATACACCGCGCGCTCCAACCTTGTAGCGGTAATCTCCAACGGCACCGCG
ATCCTCGGCCTCGGCAATCTCGGCGCGCTGGCATCCAAGCCGGTGATGGAAGGCAAGGCGGTGCTGTTCAAGCGCTTCGC
CGATGTCGACTCGATCGATATCGAGCTCGACACCGAAGACCCGGAAGCCTTCATCAATGCGGTAGCGCTGATGGAGCCGA
CGTTCGGCGGCATCAATCTCGAGGACATCGCCGCGCCCGAATGCTTCATCATCGAGCAAGCCCTGCGCGAGCGCATGAAC
ATCCCCGTCATGCATGACGACCAGCACGGCACCGCGATCATCGCCGCCGCCGGACTCATCAATGCCTGCAAGCTAACCGG
CCGCGAGCTGAGAGATTGCCGCATGGTGGTGAACGGGGCGGGGGCTTCGGCGCTCGCCTGCACGGCACTGATCAAGGCGA
TCGGCATCCCGCACGAAAACGTCATCGTGTGCGACCGTGCGGGGCCGATCTATCCGGGACGCGAGAATGTCGACCAGTGG
AAAAGCGCGCATGCCGTGCCTACCGAAGCGCGCAGCCTCGAAGAGGCGCTGGTGGGGGCAGACATTTTCCTCGGCCTGTC
GGCGGCGGGCGCATTGAAGCCCGAATGGGTAACGAAAATGGCCGATCAGCCGATCATTTTCGCCATGGCCAATCCCGTGC
CGGAGATCATGCCGGACGAAGCCAAGAAGGTCCGCCCCGATGCGATCATCGCCACCGGCCGCAGCGACTTTCCCAATCAG
GTCAACAATGTTCTCGGCTTCCCGTTCATCTTTCGCGGCGCGCTCGACGTGCGGGCGACAACGATCAACGAGGAAATGAA
GGTCGCCGCTGCCGAGGCCATCGCCGATCTCGCGCGCGAGCGCGTGCCTGAGGAAGTCGCCGCCGCCTATGGCATCAATC
ACAAATTCGGCACCGACTACATCATCCCGGCGCCTTTCGATCCGCGCCTGATGGAGGTGGTGTCCTCTGCCGTCGCCAAG
GCGGCGATGGACACCGGCGTCGCGCAGGACAGCATCGCCGATTTCGACGAATACCGCACCACGCTGAAGGCGCGGCTCAA
CCCGACCACGTCGGCGCTCACGCGGGTATACGAAGAATGCAAGGCCAACCCGGCGCGCATGGTCTTCGCAGAGGCGGAAG
AGGACGTCGTGCTGCGCGCGGCGATCCAGTACCGCGATTTCGGTTATGGCACGCCGATCCTCGTCGGGCGGACCAAGAAT
GTCGTGGACCGACTGCACATGCTCGGGGTGTCCGATCCCGGCAGTTTCGAGATCCAGAACTCGAACGATTCCGATTTCGT
CCCGGCGATGGTCGATTTTCTCTACAAGCGCCTGCAGCGCAAGGGCTATACCGAACGTGACGTGCGCCGGATGGTCAATC
AGGAACGCAACGTCTTTTCCGCGCTGCTGGTCGCGCTCGGCCATGGAGATGCAATGATTTCCGGCCTCACGCGTACCTTC
TCGCAGACTGCGCGCGAAGTGAACCGTGTGCTCGATCCCAAACCCGGCTCGGTACCGTTCGGCATCCACATGATGATCGG
CAAGAACCACACGACCTTCCTCGCCGATACCACCATCAACGAGCGGCCGAGCGCCGAGCTGCTGGCGCATATCGCCAAGG
AAACCGCGCAGGTCGCCCGCCGCATGGGGCACGAGCCGCGGGTCGCGTTCCTCAGCTATTCGACTTTCGGCAATCCCAGC
GGGCAGTGGCTCGGCAACATCCGCGACGCCGTTGCCATCCTCGACCGCGAGGAAGTGAATTTCGAATATGAAGGCGAAAT
GGCGCCCGACGCCGCGCTCAATCCCAAGGTGATGGAACTTTATCCGTTCAGCCGCCTCTCCGGCCCGGCCAATGTTCTGG
TCATGCCCGGCCTGCAATCGGCCAATCTCTCGGCAAAGCTGCTGCGCGAGCTGGCTGGCAACGCCACGATCGGCCCGATG
CTGATCGGGATGGAAAAACCGGTCCAGATCGCCCCGATGACCGCGATTGCGCCCGATGTCCTGACGCTGGCGGTGCTCGC
GGCGGCCGGGGTGGTGGGCTGA

Upstream 100 bases:

>100_bases
CGGCGGGCGGAGCGATTCGGGAAGCGGCCCGGGCAGCTTTCCCCCTATCGCAGCGCGGAGGCATTGGGTATCGCGCGGGC
AAACATGAGGAGAGCCAGCT

Downstream 100 bases:

>100_bases
GCGGCAACCCATGTCGCACATCCCGAACCAGCGGCACCGTTTCGCGATTCCCGAGGAAGTGCATTACCTCAACAACGCCT
ACATGGCGCCGCTCTCCTTC

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 753; Mature: 752

Protein sequence:

>753_residues
MAEEKPTGFTAREALFYHETIRPGKIEIIASKPMATQRDLSLAYSPGVAVPVEAIAEDPANAAKYTARSNLVAVISNGTA
ILGLGNLGALASKPVMEGKAVLFKRFADVDSIDIELDTEDPEAFINAVALMEPTFGGINLEDIAAPECFIIEQALRERMN
IPVMHDDQHGTAIIAAAGLINACKLTGRELRDCRMVVNGAGASALACTALIKAIGIPHENVIVCDRAGPIYPGRENVDQW
KSAHAVPTEARSLEEALVGADIFLGLSAAGALKPEWVTKMADQPIIFAMANPVPEIMPDEAKKVRPDAIIATGRSDFPNQ
VNNVLGFPFIFRGALDVRATTINEEMKVAAAEAIADLARERVPEEVAAAYGINHKFGTDYIIPAPFDPRLMEVVSSAVAK
AAMDTGVAQDSIADFDEYRTTLKARLNPTTSALTRVYEECKANPARMVFAEAEEDVVLRAAIQYRDFGYGTPILVGRTKN
VVDRLHMLGVSDPGSFEIQNSNDSDFVPAMVDFLYKRLQRKGYTERDVRRMVNQERNVFSALLVALGHGDAMISGLTRTF
SQTAREVNRVLDPKPGSVPFGIHMMIGKNHTTFLADTTINERPSAELLAHIAKETAQVARRMGHEPRVAFLSYSTFGNPS
GQWLGNIRDAVAILDREEVNFEYEGEMAPDAALNPKVMELYPFSRLSGPANVLVMPGLQSANLSAKLLRELAGNATIGPM
LIGMEKPVQIAPMTAIAPDVLTLAVLAAAGVVG

Sequences:

>Translated_753_residues
MAEEKPTGFTAREALFYHETIRPGKIEIIASKPMATQRDLSLAYSPGVAVPVEAIAEDPANAAKYTARSNLVAVISNGTA
ILGLGNLGALASKPVMEGKAVLFKRFADVDSIDIELDTEDPEAFINAVALMEPTFGGINLEDIAAPECFIIEQALRERMN
IPVMHDDQHGTAIIAAAGLINACKLTGRELRDCRMVVNGAGASALACTALIKAIGIPHENVIVCDRAGPIYPGRENVDQW
KSAHAVPTEARSLEEALVGADIFLGLSAAGALKPEWVTKMADQPIIFAMANPVPEIMPDEAKKVRPDAIIATGRSDFPNQ
VNNVLGFPFIFRGALDVRATTINEEMKVAAAEAIADLARERVPEEVAAAYGINHKFGTDYIIPAPFDPRLMEVVSSAVAK
AAMDTGVAQDSIADFDEYRTTLKARLNPTTSALTRVYEECKANPARMVFAEAEEDVVLRAAIQYRDFGYGTPILVGRTKN
VVDRLHMLGVSDPGSFEIQNSNDSDFVPAMVDFLYKRLQRKGYTERDVRRMVNQERNVFSALLVALGHGDAMISGLTRTF
SQTAREVNRVLDPKPGSVPFGIHMMIGKNHTTFLADTTINERPSAELLAHIAKETAQVARRMGHEPRVAFLSYSTFGNPS
GQWLGNIRDAVAILDREEVNFEYEGEMAPDAALNPKVMELYPFSRLSGPANVLVMPGLQSANLSAKLLRELAGNATIGPM
LIGMEKPVQIAPMTAIAPDVLTLAVLAAAGVVG
>Mature_752_residues
AEEKPTGFTAREALFYHETIRPGKIEIIASKPMATQRDLSLAYSPGVAVPVEAIAEDPANAAKYTARSNLVAVISNGTAI
LGLGNLGALASKPVMEGKAVLFKRFADVDSIDIELDTEDPEAFINAVALMEPTFGGINLEDIAAPECFIIEQALRERMNI
PVMHDDQHGTAIIAAAGLINACKLTGRELRDCRMVVNGAGASALACTALIKAIGIPHENVIVCDRAGPIYPGRENVDQWK
SAHAVPTEARSLEEALVGADIFLGLSAAGALKPEWVTKMADQPIIFAMANPVPEIMPDEAKKVRPDAIIATGRSDFPNQV
NNVLGFPFIFRGALDVRATTINEEMKVAAAEAIADLARERVPEEVAAAYGINHKFGTDYIIPAPFDPRLMEVVSSAVAKA
AMDTGVAQDSIADFDEYRTTLKARLNPTTSALTRVYEECKANPARMVFAEAEEDVVLRAAIQYRDFGYGTPILVGRTKNV
VDRLHMLGVSDPGSFEIQNSNDSDFVPAMVDFLYKRLQRKGYTERDVRRMVNQERNVFSALLVALGHGDAMISGLTRTFS
QTAREVNRVLDPKPGSVPFGIHMMIGKNHTTFLADTTINERPSAELLAHIAKETAQVARRMGHEPRVAFLSYSTFGNPSG
QWLGNIRDAVAILDREEVNFEYEGEMAPDAALNPKVMELYPFSRLSGPANVLVMPGLQSANLSAKLLRELAGNATIGPML
IGMEKPVQIAPMTAIAPDVLTLAVLAAAGVVG

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=352, Percent_Identity=26.9886363636364, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI62420882, Length=348, Percent_Identity=26.4367816091954, Blast_Score=74, Evalue=7e-13,
Organism=Homo sapiens, GI62420880, Length=348, Percent_Identity=26.4367816091954, Blast_Score=74, Evalue=7e-13,
Organism=Homo sapiens, GI239049447, Length=348, Percent_Identity=26.4367816091954, Blast_Score=74, Evalue=7e-13,
Organism=Escherichia coli, GI1788806, Length=745, Percent_Identity=48.4563758389262, Blast_Score=650, Evalue=0.0,
Organism=Escherichia coli, GI1788800, Length=323, Percent_Identity=29.1021671826625, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1788635, Length=306, Percent_Identity=29.4117647058824, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6322823, Length=268, Percent_Identity=30.5970149253731, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI78707242, Length=448, Percent_Identity=25.2232142857143, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI78707238, Length=448, Percent_Identity=25.2232142857143, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI78707240, Length=351, Percent_Identity=26.4957264957265, Blast_Score=73, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 81271; Mature: 81140

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEEKPTGFTAREALFYHETIRPGKIEIIASKPMATQRDLSLAYSPGVAVPVEAIAEDPA
CCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHCCCC
NAAKYTARSNLVAVISNGTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDIELDTED
HHHHHHHCCCEEEEEECCEEEEEECCCCCHHCCCCCCHHHHHHHHHCCCCCEEEEECCCC
PEAFINAVALMEPTFGGINLEDIAAPECFIIEQALRERMNIPVMHDDQHGTAIIAAAGLI
HHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHH
NACKLTGRELRDCRMVVNGAGASALACTALIKAIGIPHENVIVCDRAGPIYPGRENVDQW
HHHHHCCHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHH
KSAHAVPTEARSLEEALVGADIFLGLSAAGALKPEWVTKMADQPIIFAMANPVPEIMPDE
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCH
AKKVRPDAIIATGRSDFPNQVNNVLGFPFIFRGALDVRATTINEEMKVAAAEAIADLARE
HHHCCCCEEEECCCCCCCHHHHHHCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHH
RVPEEVAAAYGINHKFGTDYIIPAPFDPRLMEVVSSAVAKAAMDTGVAQDSIADFDEYRT
HCCHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TLKARLNPTTSALTRVYEECKANPARMVFAEAEEDVVLRAAIQYRDFGYGTPILVGRTKN
HHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEHHHHHHHCCCCCCCEEEECCHH
VVDRLHMLGVSDPGSFEIQNSNDSDFVPAMVDFLYKRLQRKGYTERDVRRMVNQERNVFS
HHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ALLVALGHGDAMISGLTRTFSQTAREVNRVLDPKPGSVPFGIHMMIGKNHTTFLADTTIN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCC
ERPSAELLAHIAKETAQVARRMGHEPRVAFLSYSTFGNPSGQWLGNIRDAVAILDREEVN
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
FEYEGEMAPDAALNPKVMELYPFSRLSGPANVLVMPGLQSANLSAKLLRELAGNATIGPM
EEECCCCCCCCCCCCCEEEEECHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCE
LIGMEKPVQIAPMTAIAPDVLTLAVLAAAGVVG
EECCCCCCEECCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AEEKPTGFTAREALFYHETIRPGKIEIIASKPMATQRDLSLAYSPGVAVPVEAIAEDPA
CCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCEEEECCCCCCCHHHHHCCCC
NAAKYTARSNLVAVISNGTAILGLGNLGALASKPVMEGKAVLFKRFADVDSIDIELDTED
HHHHHHHCCCEEEEEECCEEEEEECCCCCHHCCCCCCHHHHHHHHHCCCCCEEEEECCCC
PEAFINAVALMEPTFGGINLEDIAAPECFIIEQALRERMNIPVMHDDQHGTAIIAAAGLI
HHHHHHHHHHCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEHHHHH
NACKLTGRELRDCRMVVNGAGASALACTALIKAIGIPHENVIVCDRAGPIYPGRENVDQW
HHHHHCCHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHH
KSAHAVPTEARSLEEALVGADIFLGLSAAGALKPEWVTKMADQPIIFAMANPVPEIMPDE
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCH
AKKVRPDAIIATGRSDFPNQVNNVLGFPFIFRGALDVRATTINEEMKVAAAEAIADLARE
HHHCCCCEEEECCCCCCCHHHHHHCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHH
RVPEEVAAAYGINHKFGTDYIIPAPFDPRLMEVVSSAVAKAAMDTGVAQDSIADFDEYRT
HCCHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TLKARLNPTTSALTRVYEECKANPARMVFAEAEEDVVLRAAIQYRDFGYGTPILVGRTKN
HHHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEHHHHHHHCCCCCCCEEEECCHH
VVDRLHMLGVSDPGSFEIQNSNDSDFVPAMVDFLYKRLQRKGYTERDVRRMVNQERNVFS
HHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ALLVALGHGDAMISGLTRTFSQTAREVNRVLDPKPGSVPFGIHMMIGKNHTTFLADTTIN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCC
ERPSAELLAHIAKETAQVARRMGHEPRVAFLSYSTFGNPSGQWLGNIRDAVAILDREEVN
CCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
FEYEGEMAPDAALNPKVMELYPFSRLSGPANVLVMPGLQSANLSAKLLRELAGNATIGPM
EEECCCCCCCCCCCCCEEEEECHHHCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCE
LIGMEKPVQIAPMTAIAPDVLTLAVLAAAGVVG
EECCCCCCEECCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]