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The map label for this gene is argF [H]

Identifier: 85375611

GI number: 85375611

Start: 2805819

End: 2806763

Strand: Direct

Name: argF [H]

Synonym: ELI_13920

Alternate gene names: 85375611

Gene position: 2805819-2806763 (Clockwise)

Preceding gene: 85375610

Following gene: 85375612

Centisome position: 91.92

GC content: 64.34

Gene sequence:

>945_bases
ATGCCCGCACCGGCGACAGTACGGCATTTTCTCGACCTGTCGGATGCCGGCGGCGATGCGATCGCCGCGATGATTAATGA
CGCGCAGGACCGCAAGGCCGCGCGTACAGCCTGGCCGAAGGGGCGCCCCGATCCGGATGCGCCGCTGAAGGACCACGTCC
TCGCCATGATCTTCGAGAAAAGCTCGACCCGCACCCGCGTCAGTTTCGACATGGCGATGCGGCAACTCGGCGGCAGCGCG
ATGGTGATGGAATCGGGTTCCATGCAGCTCGGGCGGGGCGAAAGTATTGCCGATACCGCACGGGTGTTGAGCCGGATGGT
GGATGCGATCATGATCCGCACCGACGATCACGGCAAGATCGAGGAGCTGGCGCACCACGCCACGGTACCGGTGATCAACG
GCCTGACCGATCGTTCGCATCCGTGCCAGATCGTCGCCGACCTGCTGACGGTCATAGAAAATGGCAAGGCACTGCCCGGT
CTGGAGATCGCCTGGCTCGGCGATGGCAACAATGTGCTGCACTCGATCCTCGAAGCAGCGGGCCTGATGAAGTTCAACGT
CCGGGTCGGGACGCCCAAGGGCTACGAGCCCGATGGCGAATTCGTCGAATTGGCGCGTGCGGGCGGGGCGCAGGTCTCGC
TGGCTGCCGATGCGGCCGAAGCGGCGCGCGGGGCGGATGTCGTAATTACCGATACCTGGGTGTCGATGGGGCAGGAGCAT
GCTGCAACCAAGCTGGAGGCAATGGCGCCGTTCCAGGTCGACGACCGCCTGATGGACCAAGCCAAGCCGGACGCCATCTT
CCTGCACTGCCTGCCCGCGCATGTCGGGGAGGAAGTGGCCGAAAGCGTGATCGAAGGGCCGCAATCGCGGGTTTTCGACG
AGGCAGAGAACCGTATCCATGCGCAGAAATCGGTGCTGCTCTGGGCGCTCGGCAAGCTCGGCTAA

Upstream 100 bases:

>100_bases
GGGTTCTCCCGCCGCTGGTCATCGGCGATGCGGAGATCGATGAATTTTTCGACAAACTTTCCGCGGGTGCGGCAAGCTAC
AAGGTGCCCGAGGCGGCGTG

Downstream 100 bases:

>100_bases
AGGGCCCGCCTTCCAAACAAGGCAGTGCGCCCCATATAGGCGCCATGCAACAACCCGACGAAACTTACGCTGACCGCCTG
CTCAGCTTCACGATCCCTTC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MPAPATVRHFLDLSDAGGDAIAAMINDAQDRKAARTAWPKGRPDPDAPLKDHVLAMIFEKSSTRTRVSFDMAMRQLGGSA
MVMESGSMQLGRGESIADTARVLSRMVDAIMIRTDDHGKIEELAHHATVPVINGLTDRSHPCQIVADLLTVIENGKALPG
LEIAWLGDGNNVLHSILEAAGLMKFNVRVGTPKGYEPDGEFVELARAGGAQVSLAADAAEAARGADVVITDTWVSMGQEH
AATKLEAMAPFQVDDRLMDQAKPDAIFLHCLPAHVGEEVAESVIEGPQSRVFDEAENRIHAQKSVLLWALGKLG

Sequences:

>Translated_314_residues
MPAPATVRHFLDLSDAGGDAIAAMINDAQDRKAARTAWPKGRPDPDAPLKDHVLAMIFEKSSTRTRVSFDMAMRQLGGSA
MVMESGSMQLGRGESIADTARVLSRMVDAIMIRTDDHGKIEELAHHATVPVINGLTDRSHPCQIVADLLTVIENGKALPG
LEIAWLGDGNNVLHSILEAAGLMKFNVRVGTPKGYEPDGEFVELARAGGAQVSLAADAAEAARGADVVITDTWVSMGQEH
AATKLEAMAPFQVDDRLMDQAKPDAIFLHCLPAHVGEEVAESVIEGPQSRVFDEAENRIHAQKSVLLWALGKLG
>Mature_313_residues
PAPATVRHFLDLSDAGGDAIAAMINDAQDRKAARTAWPKGRPDPDAPLKDHVLAMIFEKSSTRTRVSFDMAMRQLGGSAM
VMESGSMQLGRGESIADTARVLSRMVDAIMIRTDDHGKIEELAHHATVPVINGLTDRSHPCQIVADLLTVIENGKALPGL
EIAWLGDGNNVLHSILEAAGLMKFNVRVGTPKGYEPDGEFVELARAGGAQVSLAADAAEAARGADVVITDTWVSMGQEHA
ATKLEAMAPFQVDDRLMDQAKPDAIFLHCLPAHVGEEVAESVIEGPQSRVFDEAENRIHAQKSVLLWALGKLG

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=296, Percent_Identity=43.2432432432432, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI18105007, Length=272, Percent_Identity=26.4705882352941, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1790703, Length=333, Percent_Identity=37.8378378378378, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1786469, Length=333, Percent_Identity=37.5375375375375, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI2367364, Length=321, Percent_Identity=28.6604361370716, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI48994908, Length=296, Percent_Identity=23.9864864864865, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI193204318, Length=139, Percent_Identity=35.2517985611511, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322373, Length=319, Percent_Identity=35.7366771159875, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6322331, Length=273, Percent_Identity=28.2051282051282, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24642586, Length=307, Percent_Identity=26.0586319218241, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 33636; Mature: 33504

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPATVRHFLDLSDAGGDAIAAMINDAQDRKAARTAWPKGRPDPDAPLKDHVLAMIFEK
CCCCHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEECC
SSTRTRVSFDMAMRQLGGSAMVMESGSMQLGRGESIADTARVLSRMVDAIMIRTDDHGKI
CCCCEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCHH
EELAHHATVPVINGLTDRSHPCQIVADLLTVIENGKALPGLEIAWLGDGNNVLHSILEAA
HHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
GLMKFNVRVGTPKGYEPDGEFVELARAGGAQVSLAADAAEAARGADVVITDTWVSMGQEH
CCEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCCEEEEHHHHHCCHHH
AATKLEAMAPFQVDDRLMDQAKPDAIFLHCLPAHVGEEVAESVIEGPQSRVFDEAENRIH
HHHHHHHCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AQKSVLLWALGKLG
HHHHHHHHHHHCCC
>Mature Secondary Structure 
PAPATVRHFLDLSDAGGDAIAAMINDAQDRKAARTAWPKGRPDPDAPLKDHVLAMIFEK
CCCHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHEECC
SSTRTRVSFDMAMRQLGGSAMVMESGSMQLGRGESIADTARVLSRMVDAIMIRTDDHGKI
CCCCEEHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCHH
EELAHHATVPVINGLTDRSHPCQIVADLLTVIENGKALPGLEIAWLGDGNNVLHSILEAA
HHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHH
GLMKFNVRVGTPKGYEPDGEFVELARAGGAQVSLAADAAEAARGADVVITDTWVSMGQEH
CCEEEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHCCCCEEEEHHHHHCCHHH
AATKLEAMAPFQVDDRLMDQAKPDAIFLHCLPAHVGEEVAESVIEGPQSRVFDEAENRIH
HHHHHHHCCCCCHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
AQKSVLLWALGKLG
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA