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The map label for this gene is cspA [H]

Identifier: 85375609

GI number: 85375609

Start: 2803742

End: 2804503

Strand: Direct

Name: cspA [H]

Synonym: ELI_13910

Alternate gene names: 85375609

Gene position: 2803742-2804503 (Clockwise)

Preceding gene: 85375606

Following gene: 85375610

Centisome position: 91.85

GC content: 64.44

Gene sequence:

>762_bases
ATGGGTTACGATAAAGGGCGCCGTCGCGGCAGGGACAAGCGAGACGGCTTCGGCGAAGACGGTTTCGATCCTTTCGGTGG
CGGCGGCGATTTCGGCGGTCCTCCGCCTGATCGTTTCGGAGGCGGAGGCGCCGGCTACGGAGATCGTGGCGGCGGCGGTT
TTGGCGATCGCGATGGCGGCGGTCGTGGCGGTTTCGGGGGCGGACCCCGTGGCGGCGGCGGCGGTCCTCGCGGCGGCGGC
GGCGGTGGTGGTGGTTTCGACCGCATGCCTCCCCAGGTTGTCGGAACCGGCAAGGGCACGGTGAAATTTTTCAACGGCCA
GAAAGGTTTCGGCTTCATCCAGCAGGAAGGTGGCGGAGAAGATGTGTTCGTGCACATCAGTGCTGTCGAACGCGCCGGAC
TGGATGGCCTTGCCGAAGGCCAAGAGCTCGAATTCAACCTCGTCGATCGCGGCGGAAAGGTCTCCGCTCAGGATCTGCAG
GTCGTCGGCGACGTTATCGCGGTCGAATCGCGCGACTCGGGCCCGCCCAAGCGCGAACTGACCGGCGAGAAAGCGACCGG
CACGGTCAAGTTCTTCAACTCGATGAAGGGTTTCGGCTTCCTCGTTCGCGACGACGGCCAGCCCGATGCATTCGTGCACA
TCAGCGCGGTCGAACGCTCGGGCCTGTCCGGCATCGACGAAGGCGAGCGTTACGAGTTCGACCTCGAAGTCGATCGACGC
GGCAAGTATTCGGCGGTCAATCTCGTGCCTGTTCAAGAGTGA

Upstream 100 bases:

>100_bases
GATGCCCGGCATCTCGCTTCCAAAGGACGAAGGAGCATTGCCGTCCAGCTCCTCCGACCAGAGTGTGATGGGGCGTGCGT
GGTAGGGTACTGTTTGAATT

Downstream 100 bases:

>100_bases
CCCGGCTTTGCGCTCATCCCATTTGACCGGGGTTGAGCGACAATCTAAACAGCGCGAAATGGCGGCCTCGTTTGGGGCCG
CCATTTCCCTTTGAGCCGAA

Product: cold shock protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MGYDKGRRRGRDKRDGFGEDGFDPFGGGGDFGGPPPDRFGGGGAGYGDRGGGGFGDRDGGGRGGFGGGPRGGGGGPRGGG
GGGGGFDRMPPQVVGTGKGTVKFFNGQKGFGFIQQEGGGEDVFVHISAVERAGLDGLAEGQELEFNLVDRGGKVSAQDLQ
VVGDVIAVESRDSGPPKRELTGEKATGTVKFFNSMKGFGFLVRDDGQPDAFVHISAVERSGLSGIDEGERYEFDLEVDRR
GKYSAVNLVPVQE

Sequences:

>Translated_253_residues
MGYDKGRRRGRDKRDGFGEDGFDPFGGGGDFGGPPPDRFGGGGAGYGDRGGGGFGDRDGGGRGGFGGGPRGGGGGPRGGG
GGGGGFDRMPPQVVGTGKGTVKFFNGQKGFGFIQQEGGGEDVFVHISAVERAGLDGLAEGQELEFNLVDRGGKVSAQDLQ
VVGDVIAVESRDSGPPKRELTGEKATGTVKFFNSMKGFGFLVRDDGQPDAFVHISAVERSGLSGIDEGERYEFDLEVDRR
GKYSAVNLVPVQE
>Mature_252_residues
GYDKGRRRGRDKRDGFGEDGFDPFGGGGDFGGPPPDRFGGGGAGYGDRGGGGFGDRDGGGRGGFGGGPRGGGGGPRGGGG
GGGGFDRMPPQVVGTGKGTVKFFNGQKGFGFIQQEGGGEDVFVHISAVERAGLDGLAEGQELEFNLVDRGGKVSAQDLQV
VGDVIAVESRDSGPPKRELTGEKATGTVKFFNSMKGFGFLVRDDGQPDAFVHISAVERSGLSGIDEGERYEFDLEVDRRG
KYSAVNLVPVQE

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=53, Percent_Identity=50.9433962264151, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1786841, Length=52, Percent_Identity=55.7692307692308, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI2367114, Length=70, Percent_Identity=41.4285714285714, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 26077; Mature: 25946

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYDKGRRRGRDKRDGFGEDGFDPFGGGGDFGGPPPDRFGGGGAGYGDRGGGGFGDRDGG
CCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GRGGFGGGPRGGGGGPRGGGGGGGGFDRMPPQVVGTGKGTVKFFNGQKGFGFIQQEGGGE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCEEEEECCCCC
DVFVHISAVERAGLDGLAEGQELEFNLVDRGGKVSAQDLQVVGDVIAVESRDSGPPKREL
EEEEEEEEHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEECCCCCCCHHHC
TGEKATGTVKFFNSMKGFGFLVRDDGQPDAFVHISAVERSGLSGIDEGERYEFDLEVDRR
CCCCCCCEEEEHHCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCC
GKYSAVNLVPVQE
CCEEEEEEEECCC
>Mature Secondary Structure 
GYDKGRRRGRDKRDGFGEDGFDPFGGGGDFGGPPPDRFGGGGAGYGDRGGGGFGDRDGG
CCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GRGGFGGGPRGGGGGPRGGGGGGGGFDRMPPQVVGTGKGTVKFFNGQKGFGFIQQEGGGE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCCEEEEECCCCC
DVFVHISAVERAGLDGLAEGQELEFNLVDRGGKVSAQDLQVVGDVIAVESRDSGPPKREL
EEEEEEEEHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHEEEEECCCCCCCHHHC
TGEKATGTVKFFNSMKGFGFLVRDDGQPDAFVHISAVERSGLSGIDEGERYEFDLEVDRR
CCCCCCCEEEEHHCCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCEEEEEEEECCC
GKYSAVNLVPVQE
CCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA